LOCUS       AP031283                5938 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE32 plasmid pNUITMV32_7 DNA, complete
            sequence.
ACCESSION   AP031283
VERSION     AP031283.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639561
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE32
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 99x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE32"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV32_7"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            417..596
                     /locus_tag="NUITMVRE32_31770"
     CDS             417..596
                     /locus_tag="NUITMVRE32_31770"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10099.1"
                     /translation="MFPKQYFMNYNKNLLSHIVYFNYIIIGVYHLNLTQRRDYFETQKE
                     NLSRSPLFLDDWNF"
                     /name="hypothetical protein"
     gene            580..1680
                     /gene="scpA"
                     /locus_tag="NUITMVRE32_31780"
     CDS             580..1680
                     /gene="scpA"
                     /locus_tag="NUITMVRE32_31780"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000827746.1"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="BFH10100.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="scpA"
     gene            1653..2096
                     /locus_tag="NUITMVRE32_31790"
     CDS             1653..2096
                     /locus_tag="NUITMVRE32_31790"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10101.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            3266..4234
                     /locus_tag="NUITMVRE32_31800"
     CDS             3266..4234
                     /locus_tag="NUITMVRE32_31800"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFH10102.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            4251..4775
                     /locus_tag="NUITMVRE32_31810"
     CDS             4251..4775
                     /locus_tag="NUITMVRE32_31810"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFH10103.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            5151..5432
                     /locus_tag="NUITMVRE32_31820"
     CDS             5151..5432
                     /locus_tag="NUITMVRE32_31820"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390111.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10104.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            5434..5850
                     /locus_tag="NUITMVRE32_31830"
     CDS             5434..5850
                     /locus_tag="NUITMVRE32_31830"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390085.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10105.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     Typing          3267..4230
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgcttatgca acttcttctt tttgtatttt gaggcgctac cccaatcccc aagtatcggt
       61 tatttaatag cgcggaaatc caaagaatag gacgtaccct gaaacaaaaa aagactgctc
      121 tcacgagcag cccaaaatga gattatccta ttcatcaaca taaaaatatt acaaagtgaa
      181 gaactagaga aagacaaaaa aagcatatca aatcctctca aaaataacaa gtctttaaaa
      241 aatgacagat aaaaatgcac aaagaactaa aaaagagaaa tcgcgtaaaa tcaagcatta
      301 gaagctagta actagctaga ccaattaaaa atcacaaagt gagcgtctct atccattggt
      361 agaggggcta cttggcatag tacacattat gttaactagc tcgataaata acgattatgt
      421 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
      481 attatataat tataggtgta taccacttaa atttaacaca aagacgtgat tattttgaaa
      541 cccaaaaaga aaatttatct aggtctcctc ttttccttga tgattggaat ttttagtata
      601 ggaattacaa atacctatgc ttcagaaaat tctaatttca atatcaactc ccaaccaatt
      661 gaagaacaag caattaattt tgcgaaaact gattttgaac actccataac ttcaattctt
      721 ttaatggaaa ataacaccga aactgatatt aactatactc ttggttcgcc atttaaaatg
      781 ctcgatggag aaaatacggc aaatagttct attttctttc cagtattttc agacgctgat
      841 aatacgatta aatatattta taccgtcaaa aaaaatacaa atggagaata ttcaggaagt
      901 atctcacaat ttttagcaaa agaacttcaa aatctagtag atagtaatat aacagataca
      961 accttatttt ttcaagataa tagcatttat tattccgata atggacaaat taaactactt
     1021 tggaaatcac ctttaagtac agaaaaaaat ggtgtttctt cctttacaga aaaaaatgat
     1081 cttatatccg taaatataaa aaataaaatt aaaaatttct ctaaaaaaca aaattttcct
     1141 gcggttaaac gtgtaaaaag tgtctcctcc ggagataact ttattatgat taattggcgt
     1201 atagcagaaa cccaaacaag ttttccgtgg tgtgctgctt acgtagaagc tgctatctta
     1261 tgtaacaaaa cagatgtacg tgctacttca gctagagcca ttatgcatta tacctatcct
     1321 aatttaaaca acagtcaaat tgaatcaaaa agtcttaatc aagatcaaat aataaaatat
     1381 gcaaattact gtggtgttta tcctaaaaaa gtagcacgaa tactaaacct ttcagaggtc
     1441 caaacacaac taagaaaagg aaatgccatt tatatgggaa ctgctggtta tggaaattat
     1501 agtaacagcc gtcatgcact tgcattattt gggtgggtca atcaaggtgg ttatcaaact
     1561 tactatgtgt ggaatccttg gtataactac ccaactgtaa taagtgcaaa tgcaggacca
     1621 gttacaattc ctgtacctgg aggtggtttt acttgggaca ataccattac aaattggtaa
     1681 ataaacccat tgctttagtt atgggtctag tgttagttct tttcttaact agttgttcaa
     1741 aaaaacaaga aatacaaaag actgatctga ttccttttct aggaacttgg caaactaaag
     1801 atcattcaga acgtctagta atcactaatt taaatacttc aaaaaatata gaacaatata
     1861 ccatcttagt aaaagagaac aatcataaaa ttcaaatcat tgataaaaat ttaaatataa
     1921 atgaacaagt tttcatttac aaagcaacaa ataatttact aacactgcgc tctaataaaa
     1981 aacaattaga ttacactttt agtttggatt ctccaaaaaa gctcgtattt attttgacaa
     2041 cgaatgatgg gacagaaggg acagctaaac caattgaatt ttttaaagct gattgatgaa
     2101 tagttgtttg atattacaaa acaataattt caccgaaggt gaaacaagcg aaactttaca
     2161 aaaagtggct agcatttgct agcctttttt gtattgtgga gcgtgggttt tgagggggat
     2221 tcaaggggga gcttgcgccc ctttgacaag cggttatttg caccaaatac gtaggtattt
     2281 ggctagcaaa tatagagctt gccaaacact ttagtgagtt ttgtagaaag tacctttaaa
     2341 ggtactttca cattacgaac gatacgtccg taggacgtac tgaaagggtg ggatttatga
     2401 gcgaactaat gcaaaatttg gctagcaaaa aaagttaaaa aatatatgct aataatcgag
     2461 cgaaatgaac actttttcaa gtgagcgaat ccgccatgac aaagctgaag cagtttattt
     2521 cagctttgtc atggcgtgtg cctagacttt taaaaaagtc ggcacacgag caaatatctt
     2581 tatcaagctc taatttgcgt tttaagacgt ttcaaacgtc taagtatata aatatataag
     2641 agtaggttta agagtgctta aatcgaattt tagagggcat tcagacaact ttagggttgt
     2701 tagggcttgc cctgaccgcc tgtaaggcgc tttaagaaca agataaggga tttatactaa
     2761 gactgtctta aaacgtctca aacgaaggct catagcttct caaagagttc cttatgaaca
     2821 tttttagcaa aggagccgga agcgactgcg cagtataatg tgagagcaga gcgatcacac
     2881 ctttttaagc gactgtaggg agtaagattc ccagttgaga aaaatgcgcg aatctgtttg
     2941 gagaaaaaag aagtggtttt ttaggcggga aattttgtcc ccctctttta taatctcttt
     3001 ttaaacctct ttttaaacct ctttaccgtc ggctatatcc ttactctccc aagggaatga
     3061 cgcttttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tatctacatc
     3121 gttttgtctg tttatctaca tcgttttgtc tgtttgtaca ttgaattagt acatcgacga
     3181 tgttaatata agatgtagat aaacaggcat tttgtatttc ataacgtgta tctcttgtgc
     3241 gagtaagaaa ggagctattt taaatatgtc taatgaaatc gtaaagtatc atcatgaatt
     3301 aaatacaatt ccgttgcgaa aatttacacc agtcgaaatg aatttgttct tttctatcgt
     3361 atcaagaatg cgtgatgtag gtgataaaaa agtacaattt acattcgagc aattaaaaga
     3421 tttaagcaat tataaagcaa ctgcaaatgt tcgattcata gatgatttag aaacaacgta
     3481 tgacaaatta atggatttac gttttggtag aagaagtgct gacggtttgc aacgtgaacg
     3541 ttttgtttta tttaatcaat ttaaaatcga cggaaaatct gatattcctt ttgctgaaat
     3601 acaagttcat gaaaaagcct tacccttact taataatctt gaagaatggg tgcgctattc
     3661 tctccaacaa ttcaatgaat tagaaagcag ttattcaaaa acaatgtttc gattactaaa
     3721 acaatacaga accaaaggtt tcgcttattt ttcaaaagaa gactttcatg aattacttga
     3781 tattcctaaa agttacaacc aaggtacgat tgatcaacgt gtgttcaatc caatacgaca
     3841 agaactaacc gcaatcttta aaggtttaac aatcaaaaag aaatacggaa aaggacgggg
     3901 aaagcctgtc attggctatc aatttacatt taaacctgag ataaaaaaat cagacgactt
     3961 taacaaacat gccccaacaa aagaagaaca aaacaaagca ttagaactag ctaaccaaca
     4021 agttcaatct gaaaaagcac ctacttcatt acaagaaaaa atcaaagaac aaaagcgaaa
     4081 attggaactt ttacaacagt tagaaaagtt agaaagagaa caaaataccc aaaataaaga
     4141 aaataatgca caaaaatcag agataaattc cgatattcct tcagaactaa aaaaagactt
     4201 attagacaat cttcaaaact tattcaaaga ttgaggggtt tttagatttt atgacagaaa
     4261 aaacaatcaa agaacttgct gaagaattag gagttagtaa aacagcaatt aacaaaaaag
     4321 tttctgacga aaatcgaaaa ctttggtttt cgaaaatcgg aaacaagttt gtgattaatg
     4381 aaaccggaca aaaggctata aaatcaatgt tttttaacga aaacgaaaac tcaaatcaac
     4441 aactagttgg cgaaaaaacg aaaaccgaaa accacaaaaa cgccaaccaa gttcccgaat
     4501 tagaagtcct ttctctccta gaacaacagt taaatcaaaa agatatgcaa ctacaagaaa
     4561 aagacgagca aataaaacaa atgcaaaaat tactcgacca ccaacaacaa ctcaccctgc
     4621 aagccaacca acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat
     4681 cttcggaaaa taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa
     4741 aggcggagaa aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa
     4801 ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc catgttgcga
     4861 tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt
     4921 ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac
     4981 gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt
     5041 tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta
     5101 tatttgtatg atatacataa attatacgct gatacgaaag gaaaatttcc atggacattt
     5161 tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt caggaactca
     5221 caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta atcgaaagtg
     5281 attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta ctcgaaaaat
     5341 ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac acaaacaacc
     5401 aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac
     5461 gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac
     5521 gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc tgataccaat
     5581 acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg
     5641 acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt
     5701 acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc
     5761 cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat
     5821 tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa aaaaattcct
     5881 cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc aacttgcg
//
