LOCUS       AP031282                6161 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE32 plasmid pNUITMV32_6 DNA, complete
            sequence.
ACCESSION   AP031282
VERSION     AP031282.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639561
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE32
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 99x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE32"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV32_6"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(1098..1787)
                     /locus_tag="NUITMVRE32_31680"
     CDS             complement(1098..1787)
                     /locus_tag="NUITMVRE32_31680"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10091.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1794..2084)
                     /locus_tag="NUITMVRE32_31690"
     CDS             complement(1794..2084)
                     /locus_tag="NUITMVRE32_31690"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10092.1"
                     /translation="MTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTT
                     IDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(2032..2946)
                     /locus_tag="NUITMVRE32_31700"
     CDS             complement(2032..2946)
                     /locus_tag="NUITMVRE32_31700"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="BFH10093.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2928..3317)
                     /gene="mobC_3"
                     /locus_tag="NUITMVRE32_31710"
     CDS             complement(2928..3317)
                     /gene="mobC_3"
                     /locus_tag="NUITMVRE32_31710"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002498952.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="BFH10094.1"
                     /translation="MGLMSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_3"
     gene            complement(3758..4045)
                     /locus_tag="NUITMVRE32_31720"
     CDS             complement(3758..4045)
                     /locus_tag="NUITMVRE32_31720"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10095.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            complement(4065..4289)
                     /locus_tag="NUITMVRE32_31730"
     CDS             complement(4065..4289)
                     /locus_tag="NUITMVRE32_31730"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10096.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     gene            complement(4744..4914)
                     /locus_tag="NUITMVRE32_31740"
     CDS             complement(4744..4914)
                     /locus_tag="NUITMVRE32_31740"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10097.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            complement(4929..5462)
                     /locus_tag="NUITMVRE32_31750"
     CDS             complement(4929..5462)
                     /locus_tag="NUITMVRE32_31750"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFH10098.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(5483..>6161)
                     /locus_tag="NUITMVRE32_31760"
     misc_feature    complement(5483..>6161)
                     /locus_tag="NUITMVRE32_31760"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="partial CDS similar to WP_000145511.1:replication
                     initiation protein"
     BGC             1..6161
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5484..6161)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2036..2946)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcacttctac gaccaaagcg taaagataag attttttcat aagttgcttt taaatcttct
       61 acaaatcgta catttgcagt tgctttgtag ttacttaaat cttttagttg gtcaaacgta
      121 aaacgaactg tcttatcccc tttttcacgc attctagaca cgatggaaaa aaacaaattc
      181 atttcaaccg gtgtaaattt tcttaaagga attgtattta attcatggtg gtattttaca
      241 atctcattag ccatataatc acctcaatcc aattatgaat gacatttttt aaatgtcaag
      301 acattcatat ttgacgttca aacagacaaa atgtccttca taaacagaca aaatgtcctt
      361 cataaacaga caaaatgtcc ttcataaaca gacaaaatgt ccttcataac attctgtatc
      421 cattggtaga gtaaggacac agcttacggt aaagaggttt aaaaagaggt ttaaaaagag
      481 atataaaaag aggggggcta attttcccgc ctaaaaacca cttctttttt ctttaaacag
      541 gttcgcgcat ttttcaaaac tgccaatcca aaaccttgct tcctacggtc gccaaaaagg
      601 tgtgatcgct acgctctcac attgtactgc gcagtcgcct aaaggctcct ttgctaaaaa
      661 catgttggta aagaggcctt tgagcggttc tgaatgctta tctaagccgt tttaagacag
      721 tcttggataa aaatccttat ctagctttaa agcgccttac aggcggtcag ggcaagccct
      781 aacaacccta aaataagcga tacttaaaaa caccacacaa gtttaatttt ttaagatata
      841 ataacttgtc gcctatccga cagtcttgcg actggaagga ggtgtatcta atgtttgaat
      901 tgttcatttg cccccttctt atagaagttt gtgtctcttt attcagttat tggttgaata
      961 accgaaacaa gaagtaaaat caataaaggc gacatcaacc tgaaaaaaag ccccagagaa
     1021 tccgacctct ctggggcttt cgtgtatcta atgtttgatc atttgcatgt ttagtttaac
     1081 atgattgccg atcaaattca attttgctgt tgctgatcta accattcctt gacacttttt
     1141 agataattta atgccgtttc ttttttcgtt ggattttgta tgttcgttgc ttgctgaagc
     1201 agtgctttat aacgttcgta ggtaactgat tgttgccgtt cttttcgttc ctgttcacta
     1261 gttgaaaaag ggattgtatc actgaataaa ttataacaag cttcttctgt ctgcgtatct
     1321 ttaaaatatt gcacgtcttt tttggttcgc tcatagttgt cttgaacctc tttttgctgt
     1381 ttttctggca agttttgata aagacgagtg agtttttcca tgtttttatc cgtcaaacgt
     1441 ttttcttgat tcacaaaaat atcgttcatt tcctcataac aagtggtaaa ctcttccatt
     1501 ttttgtgtta acacttgctc ctttttcttt ttatcacatg ctgcttcata cttttgagtg
     1561 atatccccta ataaaatact tcttgaaata cgtttatttc cctctaaaac gccgaataaa
     1621 ccacttaaaa cgattgataa tgtaaatata acccctggca ataaaaacct cttaaatcga
     1681 attttagagc cttttaatga atatcttgtt tgataagcct tatagctaag ccaaataatt
     1741 cctaaaatac tggcaaaaat gagtagatag ggtaaataga atgccatttc tatttattct
     1801 ccccatacca gccaaaaggc taacagtgtc atgattaagc aacataaggg ggcaagaata
     1861 tccattgatc gctgcataat tacttttttc tgttctttat ttcgctggtt taatagcgca
     1921 tggtggaatt ttttgacttg tgaatcaatt gtagtttgta ttctttcttt ggtataaggt
     1981 gcaagaaaag cacgttgttt ctttatttca ttttctatgt cttgtaacgt tttagaacga
     2041 taattcttgt tggtagtctg ctcctctttc aacgtgttgg tcatggtttc ttcgagtcgg
     2101 tctaatttcc ggttgttcag tcgtaagagt tcgttgttct cttcgtactt cttttgcagt
     2161 ttcgcgaatg ctagctcgag ctcttcgtag ctcgttggtt cgttgttgat tgtaggctcg
     2221 ttctcgtctg tccagctcat gtaaaatcgt ctccttttcg taatcctttc ctagtttgtt
     2281 ggatcgtact tttttctttt tgtggatcaa ttcgtaagta aaatttttcc cacgattcaa
     2341 gcaatgaatg ccgaatgttt taagagaatc acaaaattgg ttatacgttc gagttttttt
     2401 gtttttcatt gtttgatcaa tcgcaaaccg aatttcgtct ttccaaacag gttcattttt
     2461 ttcttttaat ttgatttcag acatggttcg tttttcgggt ttcttccctc gttcagtgat
     2521 tgttaagtca tgttcaagac aaatctcgtc atttaggtca taacatttgt ttaaaaatgc
     2581 ttgaccatgg gcttgaaatt tgttcccagt ttccagattg acggagttaa tgacaatgtg
     2641 gttatgaata tggtctttgt ctgtatgtgt gtaaatcgcg atttggtggt caggggctat
     2701 ttttttcgca tactctgccc ctagttgatt ggctttttca gcggtaattt ctcctggtcg
     2761 aaaagactgg atcaataagt gtgcttgtac acgatcgtct tttccataca gcatgcgaat
     2821 ttgtttcatt tcgcttttgg cataatcaat gtcacagttt atccctgttt tgacggttgc
     2881 tcgtggttcg gcgtaattga tcgctcggct acaagaagtg gttcggctta ctttaactgt
     2941 tgccatatct tttgtagctc cttttgcatg atttttaggt tttcggataa gcgttgggcg
     3001 tcgccaggat caacgtcgtg ttggtgtgtg ttgcaccact tggcaatttg attgatattc
     3061 ccgccagctt tggctaaatc gcgcgcgatt gcctgagcaa tgtctggcgc cactttcggt
     3121 gtcaccagac gcgcgttttg tgccttggct ttcacgaaag ccggcacact catttgcaaa
     3181 gcttcagctg actgcttcag ctttgcaaat tcggattcgc tcactcgaaa actgatttgt
     3241 tttggctcgg atcgataggt atatttttta gaatgattgc tagccaaata ttgattttgt
     3301 tcgctcataa gtcccaccct ttcagtacgt cctacggacg tatcgttcgc aataccattg
     3361 tacctaaaaa ggtacaataa gtaaagctca ctaaagctca ctaaagggct tggcaagctc
     3421 tatatttgct agccaaatac ctacgtattt ggtgcaaata accgcttgtc aaaggggcgc
     3481 aagctccccc ttgaatcccc ctccccccca ctctccacaa tacaaaaaag actagcaaat
     3541 gctagccact ttttgtaaag tttcgcttgt ttcaccttcg gtgaaattgt tgcaaatcaa
     3601 taattaggta gagctacatt tttttcgttg ttcaaagatt cttatgccat ttactattaa
     3661 taaatctata aatatgataa tttttcggct ctataattta tgggactgat gaatcagaat
     3721 tgattcttca gtcccatttt cattttattc caaagaatta actccaaata ccaatagaag
     3781 cccatcctct atatacttga gattccttac taacaatttc ttgtaattct attaaagatt
     3841 taggtaatcc aaatttatgt tttaaaatat atcttccaag aaaactactt aatttcatac
     3901 taacttcacc aacacttttt ccctcactga tttctttaga ataaagaaag agcttttctt
     3961 ttaagtcagg atagtcatct atagttgctt cattgtatac tttactaatt gcatttaaaa
     4021 gtttttcttc tttagtaaaa tccattataa taacctccct accactacct tctaggtgcc
     4081 catggaccat gattaaccca accattaaca ataatttttc caatttctcc tttagcttga
     4141 ttccaatcta cccaacattt ttctttgtta caataaagac catttccata gtaagtagct
     4201 gcatcaactt ttattcctgt accgatgcca gctagacaag ttcctaatat tcctagaata
     4261 acacaatgtt ttaatacttt ctttttcatg attgtttttc cttttcgttt ttttaaaatt
     4321 gcttgttata ttaaataatc ttagtaattt tattatataa cattttatta tataacaatg
     4381 attaacgtca taaaaagtag ttttgaattc ataatcgttt aaataaaatt ataagatcag
     4441 ttatttaaac agctagtaga cataatgtgt atactaccaa gtaacgcctg taccaaggga
     4501 tccaggcgct gattttgtga atttttattg gtctaggtta aaagctcttt ttaaggcttg
     4561 gttttctgcg gttgtttctt tctaattctt tgtgaatatt gatcggtcat tttttaaata
     4621 cttgttattt ttgtttgtgt ttgttatgct ttttacatat ctcatattca tttattattc
     4681 ttttctaagg ttaataatag gataatctct ttcagaaaga ctgctcgtac gagcagtctt
     4741 ttcttattta tcaagttttt ctacaaatac ttcctcattt tgactgttgt agtaattagg
     4801 attgagtttt agttcccctt ttttatttcc tttgttggcc actgataata acattgggct
     4861 aaagcttaat attcctagta ggatagaacc gaatagcaag tgtttttttg tcataagtat
     4921 ttccttcttc atttccatag ctgccaccat tttttccaat tctttgtttg ttcgtccatt
     4981 gtttctgttg cttcagaaag agtcgttgaa ctattctctg aagactcttc cgtataagtc
     5041 aactgcagtt gctcctgaag tttttcgatt tgttggttgg cttgcagggt gagttgttgt
     5101 tgctggtcga gtagtttttg tatatgagaa ttattttgct ctttgagggc aagttgttct
     5161 cgtaaaaatt cattttcttc acgcaactga tctagcaact tgtttgcaac cacgtttttg
     5221 tttggtatat aagcattttt atcgttgttt tgttcatgtt tatgcaactt atctagcaac
     5281 ctttgattgt ctaagttgcg agcaagttgc aatactccag aacgcaatac aaccttataa
     5341 ccacgctcat ttttagctat catggaagaa tctaattttt ggatatgttt cgatattgct
     5401 tgcctactca tcttaaaaag atctgacaat tcctttattg taaaggtttc ttgtggttgc
     5461 atactggttg cctcctaggt tgctactgga ataaattatc taaatcagat aataaacttt
     5521 ttttctcttt ttccgaaagt gcggactctg tttgtttttc aagagctttt ttctcttttt
     5581 ctagttgttt gatttgttgg aggagttcaa gttctcgttt cttctgtttg attttttcgt
     5641 taattgttgt agcgggttct tcgtattctt tttgtttatc agctaaagcc aatgctttat
     5701 tttggtcttc tttggttggt gcatgtttgt taaagtcgtc tgcttttttg atctcaggtt
     5761 tgaacgtaaa ttgatagcca ataacaggtt ttccccgtcc ttttccgtat ttctttttga
     5821 ttgttagtcc tttgaagatt gcagttagtt cttgacgtat aggttttata actcgtttat
     5881 ctatatcagg ttgcttataa cttttaggaa tatctaataa ttcaagaaag tcttctttgg
     5941 agaaatatgc gaaacctttg gttctgtatt gctttaacag acgaaacata gtctttgcat
     6001 agccactttg tagctcgttg aattgttgta aagaatagcg tacccactcg tctaaattat
     6061 ttaacaaagg cagtgctttt tcgtaaattc gtatatctac gtaaggattt tctacgtccc
     6121 cgttgatttt aaattctgta aacattacaa acatatcgta a
//
