LOCUS       AP031281                6296 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE32 plasmid pNUITMV32_5 DNA, complete
            sequence.
ACCESSION   AP031281
VERSION     AP031281.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639561
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE32
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 99x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE32"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV32_5"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(339..509)
                     /locus_tag="NUITMVRE32_31590"
     CDS             complement(339..509)
                     /locus_tag="NUITMVRE32_31590"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10082.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(525..1058)
                     /locus_tag="NUITMVRE32_31600"
     CDS             complement(525..1058)
                     /locus_tag="NUITMVRE32_31600"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFH10083.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGTQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(1079..2029)
                     /locus_tag="NUITMVRE32_31610"
     CDS             complement(1079..2029)
                     /locus_tag="NUITMVRE32_31610"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFH10084.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            complement(2912..3601)
                     /locus_tag="NUITMVRE32_31620"
     CDS             complement(2912..3601)
                     /locus_tag="NUITMVRE32_31620"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10085.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3608..3898)
                     /locus_tag="NUITMVRE32_31630"
     CDS             complement(3608..3898)
                     /locus_tag="NUITMVRE32_31630"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10086.1"
                     /translation="MNNTLNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTT
                     MDSQVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(3846..4760)
                     /locus_tag="NUITMVRE32_31640"
     CDS             complement(3846..4760)
                     /locus_tag="NUITMVRE32_31640"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="BFH10087.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4742..5131)
                     /gene="mobC_2"
                     /locus_tag="NUITMVRE32_31650"
     CDS             complement(4742..5131)
                     /gene="mobC_2"
                     /locus_tag="NUITMVRE32_31650"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002498952.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="BFH10088.1"
                     /translation="MGLMSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     DDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_2"
     gene            complement(5444..5641)
                     /locus_tag="NUITMVRE32_31660"
     CDS             complement(5444..5641)
                     /locus_tag="NUITMVRE32_31660"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10089.1"
                     /translation="MISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQLYK
                     SGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(5659..6192)
                     /locus_tag="NUITMVRE32_31670"
     CDS             complement(5659..6192)
                     /locus_tag="NUITMVRE32_31670"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10090.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     Typing          complement(1080..2028)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3850..4760)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttgataatg tggtatagtt agaatgcatt aggaaaatat gttatattaa attcttgttg
       61 acataatgtc gactatgcca agtagccctt gtatcaaccg atacagaggc tcactttgtg
      121 attttcgatt ggtctaggtc aaaagctctt tttaagcttg attttctgcg gttgcttctt
      181 tccgtttctt tgtgaatttt taccagtcat tttttactgg gttgttattt ttaataaaat
      241 atgataattt tttggtagcg cttcataagc gcgttgcttt ttcaagaata aacactcatt
      301 attttttaac tggctgctcg aaagagcagt cttttttatt atttatctgt ttcttttaca
      361 aacaattctt cgttaggact gttgtaataa tttggattca atttcaattc accatgttta
      421 ttccctttat tgatcactga caataacatc ggactgaatc ctagaattcc tagtaaaata
      481 gaaccgaata gaagattttt tcttgtcatg aatgttccct ccagttattt ccaaagttgc
      541 caccattttt tttcagtatc aatttgtttg tctattgttt ctgttgcttc agaaagagtc
      601 gttgagtcat tctctggagc ttcttctgta taagtcaact gtagtttttc ttgcagtttt
      661 tcgatttgtt tgttggcttg tagggtgagt tgttgttgct ggtcgagtag tttttgtata
      721 tgagaattat tttgctctct gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
      781 aactgatcta gcaacttgtt tgtaaccacg tttttgcttg gtatattaac atttttatcg
      841 ttgtcttgtt cgtactcgtg caacctatct agcaacattt gggtgcctaa gttgcgagca
      901 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
      961 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagttct
     1021 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1081 actggaataa attatctaaa tcagataata aacttttttt ctcttttttc gaaagtgcgg
     1141 actctgtttg tttttcaaga gcttctttct ctttttctag ttgtttgatt tgttggagga
     1201 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagca ggttcttcgt
     1261 attctttttg tttatcagct aaagccaatg ctttattttg ttcttctttg gttggtgcat
     1321 gtttattaaa atcatctgct tttttgattt ctggtttaaa cgtaaattga tagccaacga
     1381 caggttttcc tcgccctttt ccgtatttct ttttgattga taatcctttg aagattgcgg
     1441 ttaattcctg tcgtataggc tttataacac gattatcaac gtttgcttgg ttgttccagt
     1501 aactttttgg aatatctaat agttcatgaa aatcctcttt agaaaaataa gcaaaacctt
     1561 tagttcgata ttgttttagt aatcgaaaca tagtctttgc atagctactt tgtaattcgt
     1621 taaattgttg gagtgaatag cgaacccact catctaaatt gtttagtaaa ggtaaagctt
     1681 tttcgtaaac ttgtatgtct acatatggat ctttagcagt tccacgtatt ttaaactcac
     1741 taaacataac gaatctatct atatcgagcc cattagcact tcttttacca aaacgtaaat
     1801 ccattaattt atcataagtc atttctaaat cattaatgaa acgattatta gcagttgctt
     1861 tataattact taaatctttt agttggtcaa aagtaagacg gactgtttta tcgccttttt
     1921 cgcgcattct agacacaata gagaaaaata gattcatttc aactggtgta aatttccgca
     1981 acggaatggt atttaattca tgatggtatt tcacgatttc atttgccaaa gatattcctc
     2041 cttactcctt caatggatac agacgttata aacaacgttt tgtctgtgat aatgcatatt
     2101 ataacatacg tgagtgcaaa aaaagagttt aaaagcagac aaaatgtagt ttaaaagcag
     2161 acaaaatgta gtttaaaagc agacaaaatg tagtttaaaa gcagacaaaa tgtagtttat
     2221 aaaacgctgt atccattgaa ggagtaagga cacagccgac ggtaaagagg tttaaaaaaa
     2281 ggtttaaaaa gagattataa aagagggtga caaaatttcc cgcctaaaaa ccacttcttt
     2341 tttctaccaa caggttcgcg catttttcaa aactgccaat tcaaaacctt gctccctacg
     2401 gtcgctaaaa aagtgtgatc gctatgctct cacattgtac tgcgcagtcg cctaaaggct
     2461 cctttgctaa aaacaagtca gtaacggagg tgggggaagc ctctgatagc tcgtctaagt
     2521 cgttttaaga cagtcttggg taaaaaacct tatcttgttc ttaaagcgcc ttacaggcgg
     2581 tcagggcaag ccctaacaac ccgaaaataa gggatactta aaaacaccac acagatttta
     2641 ttttgtaagg tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
     2701 tctaatgttt gaacggttca tttgccccct tcttgtagaa gtttgtgtct ctttattcag
     2761 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
     2821 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
     2881 aggttcagtt taacatgatt gctaatcaaa ttcaattttg ttgttgctga tccaaccatt
     2941 ccttgacact ttctagataa tttaatgccg tttctttttt cgttggattt tgtatgtttg
     3001 ttgcttgctg aagcagtgtt tcataacgtt cgtatgtgac cgattgttgc cgttcttttc
     3061 gttcttgttc actggttgaa aaagggattg tatcgctaaa taagttacaa caagcttctt
     3121 ctgtctgcgt atctttaaaa tattgcacgt ctttttttac ttgctcatat ttttcttgta
     3181 cctctttttg ctgtttttct ggtaagtttt gataaaaacg agtgagtttt ttcatgtttt
     3241 tatctgttaa acgtttctct tgatccacaa aaatattgtt catgtcctca taacaagcgg
     3301 taaattcctc cattttttgt gctaatgctt gctctttttt ctttttatca catgctgctt
     3361 catacttttg agtgatatcc cctaataaaa tgcttcttga aacacgttta ttaccctcta
     3421 aaacgccgaa taaaccactt aaaacgattg ataaggcaaa tataaccccg accaataaaa
     3481 atcgcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     3541 taagccaaat aattcctaaa atactggcag aaatgagtag atagggtaaa tagaatgcca
     3601 tgtctgttta ttctccccat accaaccaaa aggctaataa tgttagtact aagcaacata
     3661 agggggcaag aatatccatc gatcgttgaa ttaaagcctt tttttgttct tggtttcgct
     3721 ggtctaatag cgcttggtgg agttttttga cttgtgaatc catcgtagtt tgtatccttt
     3781 cttgggtata aggcgcaaga aaggcacgtt gtctctttat ttcgttttcg atgtctttta
     3841 acgttttaga gcgataattc ttgttggtgg tctgttcctc gttcaacgtg ttgttcatgg
     3901 tttcttcgag tcggtctaat ttccgattgt tcaggcgtaa aagttcgttg ttctcttcgt
     3961 acttcttctg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     4021 tgattgtagg ttcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     4081 tttcctagtt tgttggatcg tacttttttc tttttgtgga ccaattcgta agtaaaattt
     4141 tttccgcgat tcaagcaatg aatgccgaat gttttaagtg aatcacaaaa ttgggtatac
     4201 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     4261 accggttcat ttttttcttt taatttaatt tcagacatgg ttcgtttttc ggttttcttt
     4321 cctcgttccg tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     4381 ttgtttaaaa atgcttgccc atgggcttga aattttttcc cagtttcaag attaacggag
     4441 ttaatgacaa tgtgattatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     4501 tggttagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     4561 atttctcctg gtcgaaagga ctgaatcaat aagtgtgctt gtacacgatc gtcttttcca
     4621 taaagcgtgc gtatttgttt catttcactt ttggcataat caatgtcaca gtttattcct
     4681 gttttgacgg ttgctcgtgg ttctgcgtaa ttgattgctc ggctacatga agtggttcga
     4741 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     4801 ataaacgttg agcatcatca ggagcaacgt cgtgttggtg tgtattgcac cacttggcaa
     4861 tttgattgat attccctcca gctttggcta aatcgcgcgc gattgcctgg gcaatgtctg
     4921 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     4981 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     5041 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga tcgctagcca
     5101 aattttgatg ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     5161 tcgcaatacc attgtaccta aaaaggtaca atgagtaaag ctcactaaaa gggtttggca
     5221 agctctatat ttgctagcca aatacctccg tatttggtgc aaataaccgc ttgtcaaagg
     5281 agcgcaagct cccccttgaa tccccctcaa aatccacgct ccacaataca aaaaaaggct
     5341 agcatttgct agccactttt tgtaaagttt cgcttatttc accgaaggtg aaattgttgc
     5401 tttgtaatat tcaaacaacc ctattcttct aaaatgtatc gatttatttt tttttagact
     5461 ttattatgtc taagacaact aagattaaca ctaaaaataa accagatttg tataattgcc
     5521 ctccgcttag catattaaca attatagcaa cagctatagc aaatataaaa atacgaatag
     5581 tagctatttg cggattcttt ttagtaaatg tttttaatga ttttaatgat ttagatatca
     5641 aaaacatcac tccttaactt ataacgttga atatctagta gctacaactc ttccagctgc
     5701 atcatatccc ctttggtaat aaaatccaat ccagccatta ccatttttgt cccattcttt
     5761 tgcaactgca ttccctggag aagcaccttt tttagtccaa cttgcacctg ttaacaaagc
     5821 accaattgcc caacctggga taccaaacat agaagtgcta gctttccctc ctgcccaaaa
     5881 ggcagcatta atagcattag cagctactcc agtggttaca gcaatatttg tatattcctt
     5941 tttgccccat cttgcaactg cagctcttgt catgatatct gaagaatttg caaaatcttc
     6001 cgcactataa ccatcctcat atttagttac gaccatacca tcttcagtgt actcaataac
     6061 tacattttca gtagcattgt tttcgtttaa tgataccact tgggaatttt ctgaagcata
     6121 aactatagta gttgacggta aaacaactgc cccaatagtt aacaaagcta cccctaaagt
     6181 aaattttttc atagaatttc ctcctgatac agaaaaatat atagaaaacg ttttctttgt
     6241 gaaaatcata aaacaaaagg tataggcttg tcaatatttt atttagtaaa gataat
//
