LOCUS       AP031274                5262 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE30 plasmid pNUITMV30_7 DNA, complete
            sequence.
ACCESSION   AP031274
VERSION     AP031274.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639559
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 346x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE30"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV30_7"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            773..922
                     /locus_tag="NUITMVRE30_29900"
     CDS             773..922
                     /locus_tag="NUITMVRE30_29900"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06918.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGEQ
                     KNDG"
                     /name="hypothetical protein"
     gene            912..1073
                     /locus_tag="NUITMVRE30_29910"
     CDS             912..1073
                     /locus_tag="NUITMVRE30_29910"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06919.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLTE
                     IIELDSKE"
                     /name="hypothetical protein"
     gene            1082..1447
                     /locus_tag="NUITMVRE30_29920"
     CDS             1082..1447
                     /locus_tag="NUITMVRE30_29920"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06920.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFMR
                     NASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLDYK
                     TFYPELLEFFQKLNSLE"
                     /name="hypothetical protein"
     gene            2003..2359
                     /locus_tag="NUITMVRE30_29930"
     CDS             2003..2359
                     /locus_tag="NUITMVRE30_29930"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06921.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLCK
                     SVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQQL
                     SQIEKGVIDLWEQL"
                     /name="hypothetical protein"
     gene            2344..3330
                     /locus_tag="NUITMVRE30_29940"
     CDS             2344..3330
                     /locus_tag="NUITMVRE30_29940"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06922.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLAQ
                     TQLEATRRIFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPNHE
                     IYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQPKE
                     KFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNKGKRI
                     GFKWVDDEGKDFTIGGRKLGSDFERPAIERTLEFSQQKQEKEVEKPLETKPKVTNDDFS
                     ISGLKNLANDIRQQEERKKKEELQRQKEIERQQRSRPFYHDRGGMER"
                     /name="hypothetical protein"
     gene            3338..3793
                     /locus_tag="NUITMVRE30_29950"
     CDS             3338..3793
                     /locus_tag="NUITMVRE30_29950"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06923.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGYI
                     KVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQVQ
                     KFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
                     /name="hypothetical protein"
     gene            3821..4318
                     /locus_tag="NUITMVRE30_29960"
     CDS             3821..4318
                     /locus_tag="NUITMVRE30_29960"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06924.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRVV
                     FALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQAL
                     KIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYFELN
                     SQ"
                     /name="hypothetical protein"
ORIGIN
        1 attttcagtt tgggacaacc aaataaaaaa gctgatattg cattaaaaga cttactaagc
       61 cgtcaagatt atttagtagt gcaaggaaac gacttagcta aggcatttgg agggcttaaa
      121 tcgtttgagc aacgagtttt agactactgt tttagctttg taactaaaga ttcaaaacta
      181 gatgacgaat acgaggtaac cgcattagaa attatcaaac attttgggtt gaacacctct
      241 ggagattctt accgtcgaat agctgaagct ttcaaaaagc ttaatgaaaa tactgcgctt
      301 tatcttcgtg tcgagcgccc cgatggtact caaggtctca gaatgacgca attattttct
      361 tggattgatt tttatgctga cgggttagta agattcaaat tttctgctga ttcagctcca
      421 tacgttcttg atctcaaaga aaagttttat agctttcgtt taggtgaatt atctcggatc
      481 aatgggaaat atgctctcat aatgttaaaa ctctgggaat cctaccgtag aggaaaagaa
      541 aaacacacaa cgattacagg tgatttggaa gaatgggaat cttggtttct tggtaaagac
      601 aaacgccttc ctgcaggtca attttttcaa aaagtaatta ctcgtgctat tgaagaatta
      661 tctgataaga tgtcagtttc atttttcgtc acaaccttaa agcgtgggcg aaaagtcgtt
      721 ggatatgaaa tcacgatcaa tgacaccagc aaagaataga ggagtgattt agttgaaaaa
      781 aagactgcgt aaaaagatgg caaagaaaaa ttcttcgcta gatcaagcta tgaaagaact
      841 aatgaactat gcaaaaggcg aacgtgacgg ctttttgatt catagttatg acgaaaaagg
      901 agaacaaaaa aatgacggct aagacagtta cgattgacgt tgaacagctt gaaaagctgt
      961 tagaaaggca tagtgacgct attattgaat acaccggtgg actttcagaa gaaacgatca
     1021 accaatttga tcttcttcta acggaaatta tcgaactaga ttctaaggag tgagtttacg
     1081 catggcaaaa atggatatcg aagatttgga aaaaaaatta gctgaagctc ggcatgatct
     1141 ttcagtcaaa atcggtacaa atatggttga aaaacaaggg ttcaagagct attccgccta
     1201 caatactttc atgcggaatg cgtcaaaggc attgaagtcg tccgccggat ctgcacctgc
     1261 gccagcagct gcaaagccgg tggccacttc tgccgaaaaa ttgcctagcg agctgaaagc
     1321 cgaagcggtg caatttcaaa agcaactcga aaaatggatg gaagacaact ggggaaacaa
     1381 gttggattac aaaacatttt atcctgagtt gctagagttc ttccaaaaat tgaacagctt
     1441 ggaatagcgg aacgagctag tccatgcgat caatttttga agaacgagca agccgatttt
     1501 tggcttgctt ttttgtttgc ctgcgaacag cgggcaaaca aaaaaataag gtcaacttgc
     1561 gtgcaagttt gaccgcatga gccagtagaa aaatagtcga acagactact ttttctgagg
     1621 cgaaatcacg taataaaaat agccgaacag gctacttttt gttgcgtgat tgggggagtt
     1681 cgtagggggc aacgccacct cgacaagcga tgtatttggg taccaaatgc ggtgcttgcg
     1741 tattatatga tttattaacc ttgttatgcg accgaatagg gagtatgatg aggttaataa
     1801 atcattcggt tatagagcgg aaaaagggaa gtgaccgagt agggaacttt cattttgtag
     1861 tggcataacc gagggagatt tcattgcgga aaaaaggcga ctgaataggg agcgtttttg
     1921 tagctttgaa tatcgaccac gaagaatgac gagaagaaaa aagcgaccga atagggagca
     1981 tttttatttg aggagtgatg agatgagcga gcagaaattg aatgccgaaa atcgaaaaga
     2041 tagtcggcaa attaaatttc gagtgagcga agacgagtac cagtatttga ccgccattgc
     2101 ggatcaaact ggtatgagtg tgccacagct ctgcaagagc gtggcacttg gcatgaagta
     2161 tcgagcgccg aacgttgatc gggagggggc gatacaaatt gccaaagagt tgcgcaaata
     2221 tggcggaaat ctcaatcaga tggctcgctt tattaacatg aataaggggc aaatgccttc
     2281 aagttcagat ttagccttga tgaaccagca actttcgcaa atcgaaaagg gggtgattga
     2341 cttatgggag caactttagc tttaaaaggc ggtaaacgtg gggcttctgc gctgattggc
     2401 tatgtaaatc ggcatggaaa gcttgatcgt gaccgcattt atacgcacaa actgtcagct
     2461 gagaataaat tggctcaaac tcagctagaa gcgactagac ggattttcgc caaaacaggc
     2521 ggtattcaag cctttcattg ggttcaatcg tttgataatt cgtttgacaa aaacaatccg
     2581 gcggacgtgc aaaaagcgca tgagatcggt gctaaactcg ttgatgaaat cgcaaaaagc
     2641 tatcccaatc atgaaatcta catgggcact cataccgatt cagaatccgg ctatattcat
     2701 aatcacattg ttttcgccag tatcgacact gaaaccggta aaaaatttca ctctgatacc
     2761 gaggtcggct accacctaca agacctaaac gataaggttt tattgagttt tggcattgaa
     2821 cagccgaaag aaaaattcaa acaagttgat tttgaaatgc acgccgaggg caaaatgagt
     2881 aatcgagaaa aaatgaagca tatgatcctg ggcgcacaag atatgagtac cgattttgat
     2941 tctttcaaaa aaaatcttca agaagcctat gctgtcgagg tttatgagta caataaaggc
     3001 aaaaggattg gcttcaaatg ggtcgatgat gagggcaaag atttcacaat cggcggacga
     3061 aaattaggtt ctgattttga gcgccctgcg atcgaacgaa cacttgaatt ttctcaacaa
     3121 aaacaagaaa aagaagtcga aaaaccgctt gaaaccaagc ctaaagtcac caatgatgac
     3181 ttttctatca gcggtttgaa aaatcttgct aatgatattc gtcagcaaga agaacgcaag
     3241 aaaaaagagg agctgcaacg acaaaaagaa atcgagcgtc aacaacgttc tcgcccgttc
     3301 tatcatgatc gtggtggcat ggaaaggtag tgacgatatg ccgttagatg attttgcaag
     3361 taagcaggag cttcaaaaag tgaagcagga actagcggaa atcaagcaac aaaatcaaga
     3421 agtcattgaa acgctgaaaa gccttgataa cagcgtaaaa caaggctata tcaaggtgcg
     3481 aggtggcgaa gatattccca aaatgagcga gctgaaagct gaaatgaacc gctacgccac
     3541 ctacgttcaa aaaagaaccg ctgaaatcga ccaaaagctt gatagctacg gtaatatcag
     3601 caaatctgta tctgacgaat tgagccggca agtgcgccca caagtccaaa aatttttagc
     3661 caacgatgat cgagcgactg ccgaagagct gaaacaaaat cgaatagcga atttaatgtc
     3721 cgtattagcc tctgtggggc tttttatcgg tggcactgct attcttattg tttcagtcct
     3781 aaagctcctg taatcgaacg agagggggct taaaacgcaa atgaaactgt tctttgaaaa
     3841 aatggggtgg catattcttc ctgtcgtgat tattttggtc ggttcgtata gtttattctg
     3901 catttttcgc tcgatcttag aaaaatttga ggaaccaaag ctgaaacgtg tcgtttttgc
     3961 cctctgtttt gcgtttctag cgctttttgt gtcggtcggc atttcttcat accgtccgga
     4021 cgttgcgctg tctgtacgcc ctaaaatcag caaaatagag caactagaag cgaaagccga
     4081 agcaaaacag aaaaaaattg ataaaatttc ggaccaactg gagcaggcat tgaaaattgc
     4141 cagtcaagaa aagctgaaaa cctttaaccc aaaagactat ccaagcgaag ttattacggc
     4201 ggattgggta caaaaaaatc gtgacgtgat cgctactttg cccgataaag agctgaaaga
     4261 ccgctacaca aagcgatttg acgatgtaga atcctatttt gagctgaata gtcagtgaaa
     4321 ttgagatttg ccaacgtcca tgctataata accatgcgaa aacgttagaa ttttttcgag
     4381 tgcggaatgc acaaaagcca gacaccatgc gacggtgtct ggcttttttt tcgccctgcg
     4441 actaggcgtg tgagttggaa ggcactaagc cttagttgtc gtctttctcg tccaaccaat
     4501 gagaaactag ctcaatgact agcccgacaa atagcggtgc gacaaccaac gttagaaatt
     4561 tttcgtacat acggaataca cctcccaacc tgtgtaagcg ttagttggct ttagtgccaa
     4621 gattaatata gcattcactc cctgcaaaaa aaagatggtc taccagatgg ggcttttggg
     4681 tcgttagggc ttgccctgac cgcctgtaaa ggcgctgtat aacaagacta acgatttaat
     4741 ctaaatcagt ctaaaaactt gctacaacaa gcttttacgc ctatcccaac cgctccttga
     4801 acaacggttt tgaccttaac gaaggagcgc aggcgactgc tgtaagcaaa atgtgagagc
     4861 gtagcgatca ctcctttatg ccttgtggct gtaagattga acgaggtaaa ggggataaat
     4921 tgtcgatgat gtgagaagca gtttatttca tgcgcgaacc tgttggaaaa aaatagacaa
     4981 ctggtttggg cgggattttt ttttttttga atttaaattt ttttctcacc cgcgcgctta
     5041 aagagaatac tttatagatt acttttaaat actttaaaat actttagggg aaaaggaatg
     5101 gctttatacc agcgtttgta gcgcctataa atactatttt ccacgttgaa aatactattt
     5161 tccacgtcaa aaatactatt ttccacgtca aaaatactat tttccacgtt gaaaattcaa
     5221 atattgcaaa aaaactgaat aaatggtatt attttaaaaa aa
//
