LOCUS       AP031273                5945 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE30 plasmid pNUITMV30_6 DNA, complete
            sequence.
ACCESSION   AP031273
VERSION     AP031273.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639559
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 346x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE30"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV30_6"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(1543..1986)
                     /locus_tag="NUITMVRE30_29830"
     CDS             complement(1543..1986)
                     /locus_tag="NUITMVRE30_29830"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06911.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(1959..3059)
                     /gene="scpA"
                     /locus_tag="NUITMVRE30_29840"
     CDS             complement(1959..3059)
                     /gene="scpA"
                     /locus_tag="NUITMVRE30_29840"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000827746.1"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="BFH06912.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="scpA"
     gene            complement(3043..3222)
                     /locus_tag="NUITMVRE30_29850"
     CDS             complement(3043..3222)
                     /locus_tag="NUITMVRE30_29850"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06913.1"
                     /translation="MFPKQYFMNYNKNLLSHIVYFNYIIIGVYHLNLTQRRDYFETQKE
                     NLSRSPLFLDDWNF"
                     /name="hypothetical protein"
     gene            complement(3743..4159)
                     /locus_tag="NUITMVRE30_29860"
     CDS             complement(3743..4159)
                     /locus_tag="NUITMVRE30_29860"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390085.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06914.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(4161..4442)
                     /locus_tag="NUITMVRE30_29870"
     CDS             complement(4161..4442)
                     /locus_tag="NUITMVRE30_29870"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390111.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06915.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(4818..5342)
                     /locus_tag="NUITMVRE30_29880"
     CDS             complement(4818..5342)
                     /locus_tag="NUITMVRE30_29880"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFH06916.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(5359..5943)
                     /locus_tag="NUITMVRE30_29890"
     CDS             complement(5359..5943)
                     /locus_tag="NUITMVRE30_29890"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06917.1"
                     /translation="MRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFSKEDFHELLDI
                     PKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKSDDF
                     NKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEKLEREQNTQNK
                     ENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="hypothetical protein"
     Typing          complement(2..373)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.464"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa ggaatatcag
       61 attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc aaaccgtcag
      121 cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct aaatcatcta
      181 tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc tcgaatgtaa
      241 attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag aacaaattca
      301 tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga tactttacga
      361 tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata cacgttatga
      421 aatacaaaat gcctgtttat ctacatctta tattaacatc gtcgatgtac taattcaatg
      481 tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa cagacaaaac
      541 gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg gagagtaagg
      601 atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata aaagaggggg
      661 acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg cgcatttttc
      721 tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc tgctctcaca
      781 ttatactgcg cagtcgcttc cggctccttt gctaaaaatg ttcataagga actctttgag
      841 aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc ccttatcttg
      901 ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag ttgtctgaat
      961 gccctctaaa attcgattta agcactctta aacctactct tatatattta tatacttaga
     1021 cgtttgaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct cgtgtgccga
     1081 cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct tcagctttgt
     1141 catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag catatatttt
     1201 ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca ccctttcagt
     1261 acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact ttctacaaaa
     1321 ctcactaaag tgtttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca
     1381 aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat ccccctcaaa acccacgctc
     1441 cacaatacaa aaaaggctag caaatgctag ccactttttg taaagtttcg cttgtttcac
     1501 cttcggtgaa attattgttt tgtaatatca aacaactatt catcaatcag ctttaaaaaa
     1561 ttcaattggt ttagctgtcc cttctgtccc atcattcgtt gtcaaaataa atacgagctt
     1621 ttttggagaa tccaaactaa aagtgtaatc taattgtttt ttattagagc gcagtgttag
     1681 taaattattt gttgctttgt aaatgaaaac ttgttcattt atatttaaat ttttatcaat
     1741 gatttgaatt ttatgattgt tctcttttac taagatggta tattgttcta tattttttga
     1801 agtatttaaa ttagtgatta ctagacgttc tgaatgatct ttagtttgcc aagttcctag
     1861 aaaaggaatc agatcagtct tttgtatttc ttgttttttt gaacaactag ttaagaaaag
     1921 aactaacact agacccataa ctaaagcaat gggtttattt accaatttgt aatggtattg
     1981 tcccaagtaa aaccacctcc aggtacagga attgtaactg gtcctgcatt tgcacttatt
     2041 acagttgggt agttatacca aggattccac acatagtaag tttgataacc accttgattg
     2101 acccacccaa ataatgcaag tgcatgacgg ctgttactat aatttccata accagcagtt
     2161 cccatataaa tggcatttcc ttttcttagt tgtgtttgga cctctgaaag gtttagtatt
     2221 cgtgctactt ttttaggata aacaccacag taatttgcat attttattat ttgatcttga
     2281 ttaagacttt ttgattcaat ttgactgttg tttaaattag gataggtata atgcataatg
     2341 gctctagctg aagtagcacg tacatctgtt ttgttacata agatagcagc ttctacgtaa
     2401 gcagcacacc acggaaaact tgtttgggtt tctgctatac gccaattaat cataataaag
     2461 ttatctccgg aggagacact ttttacacgt ttaaccgcag gaaaattttg ttttttagag
     2521 aaatttttaa ttttattttt tatatttacg gatataagat catttttttc tgtaaaggaa
     2581 gaaacaccat ttttttctgt acttaaaggt gatttccaaa gtagtttaat ttgtccatta
     2641 tcggaataat aaatgctatt atcttgaaaa aataaggttg tatctgttat attactatct
     2701 actagatttt gaagttcttt tgctaaaaat tgtgagatac ttcctgaata ttctccattt
     2761 gtattttttt tgacggtata aatatattta atcgtattat cagcgtctga aaatactgga
     2821 aagaaaatag aactatttgc cgtattttct ccatcgagca ttttaaatgg cgaaccaaga
     2881 gtatagttaa tatcagtttc ggtgttattt tccattaaaa gaattgaagt tatggagtgt
     2941 tcaaaatcag ttttcgcaaa attaattgct tgttcttcaa ttggttggga gttgatattg
     3001 aaattagaat tttctgaagc ataggtattt gtaattccta tactaaaaat tccaatcatc
     3061 aaggaaaaga ggagacctag ataaattttc tttttgggtt tcaaaataat cacgtctttg
     3121 tgttaaattt aagtggtata cacctataat tatataatta aaatatacaa tatgcgataa
     3181 caaatttttg ttatagttca taaaatattg ttttgggaac ataatcgtta tttatcgagc
     3241 tagttaacat aatgtgtact atgccaagta gcccctctac caatggatag agacgctcac
     3301 tttgtgattt ttaattggtc tagctagtta ctagcttcta atgcttgatt ttacgcgatt
     3361 tctctttttt agttctttgt gcatttttat ctgtcatttt ttaaagactt gttatttttg
     3421 agaggatttg atatgctttt tttgtctttc tctagttctt cactttgtaa tatttttatg
     3481 ttgatgaata ggataatctc attttgggct gctcgtgaga gcagtctttt tttgtttcag
     3541 ggtacgtcct attctttgga tttccgcgct attaaataac cgatacttgg ggattggggt
     3601 agcgcctcaa aatacaaaaa gaagaagttg cataagcgga tttttctttt ttttcgcaag
     3661 ttggtaccaa cttgcaaatc ttgctggaag actgatccct aaaaagaaat cgaggaattt
     3721 ttttagggat cggtttcttt ttttatcttt ttagaaagtt tgttgggtca aaatttccag
     3781 aagtaaaggt ttgattggtt tctttttgac gatttttggg ttgatttcgt cgggaaggat
     3841 tgagttgatt ttcaattttt tctttcactt cttgatacac tttagaattt gtaccgataa
     3901 gaacaccagt atcttttaga tataaatcag caaattctgc catgatttct gtcatgatag
     3961 aaatttcttt tcccatggca ttgagttttg cttggttact ggttgtggtt gtattggtat
     4021 caggttcata actcatacat aaaaaacgaa tggcttctga ataattgttg acgtctggat
     4081 ttttttcgat gatggaatct aaaacgtcaa cgtgactggg ataaagggtt acgtgttttt
     4141 gaacagaagt ttttctcatg ttatattcct ccgataagtg tttcaatgag ttggttgttt
     4201 gtgtgaacat agtcattcag tatgttcgta ttttctgttt gaatggcaat aaatttttcg
     4261 agtaataaat cgaattgatc ttttttgtaa ttcataagtt gacggtcaaa atcactttcg
     4321 attagaactt ttagatattt atttcgactc cattttttgc ccgttttggt tgtgagttcc
     4381 tgacaacgtt tatctattct tgcaataatt ttgggattga tatttcgaac taaaatgtcc
     4441 atggaaattt tcctttcgta tcagcgtata atttatgtat atcatacaaa tatagtattt
     4501 gtatagctat ttttcgccaa aacactatac atagcctagc cacaatggca aaaaagtgat
     4561 agcaaatttt tttctgagaa acgtgatagg cacagcctat ctagcacctt tcgtaagaaa
     4621 gtgataccac gtttccttat gctctttcaa aattcagaaa aatcagctgt aaaacgagtg
     4681 cagtccgagt aagattcaac gaaaaaacgg ggattggggc gccaccaaaa tatcgcaaca
     4741 tgggtccatg ttgctctgct cgcaaaagca aaaagaagaa taaaaccagc aatttttatt
     4801 aatttaaatt gctggtttta ttgccagaat tgccaccatt ttttctccgc ctttgattgt
     4861 ttttctatcg attctgtttc ttcagaaaga gtcgttgatt tattttccga agattcttcc
     4921 gtataagtca actgtagttg ttcctgcagt ttctcgattt gttggttggc ttgcagggtg
     4981 agttgttgtt ggtggtcgag taatttttgc atttgtttta tttgctcgtc tttttcttgt
     5041 agttgcatat ctttttgatt taactgttgt tctaggagag aaaggacttc taattcggga
     5101 acttggttgg cgtttttgtg gttttcggtt ttcgtttttt cgccaactag ttgttgattt
     5161 gagttttcgt tttcgttaaa aaacattgat tttatagcct tttgtccggt ttcattaatc
     5221 acaaacttgt ttccgatttt cgaaaaccaa agttttcgat tttcgtcaga aacttttttg
     5281 ttaattgctg ttttactaac tcctaattct tcagcaagtt ctttgattgt tttttctgtc
     5341 ataaaatcta aaaacccctc aatctttgaa taagttttga agattgtcta ataagtcttt
     5401 ttttagttct gaaggaatat cggaatttat ctctgatttt tgtgcattat tttctttatt
     5461 ttgggtattt tgttctcttt ctaacttttc taactgttgt aaaagttcca attttcgctt
     5521 ttgttctttg attttttctt gtaatgaagt aggtgctttt tcagattgaa cttgttggtt
     5581 agctagttct aatgctttgt tttgttcttc ttttgttggg gcatgtttgt taaagtcgtc
     5641 tgattttttt atctcaggtt taaatgtaaa ttgatagcca atgacaggct ttccccgtcc
     5701 ttttccgtat ttctttttga ttgttaaacc tttaaagatt gcggttagtt cttgtcgtat
     5761 tggattgaac acacgttgat caatcgtacc ttggttgtaa cttttaggaa tatcaagtaa
     5821 ttcatgaaag tcttcttttg aaaaataagc gaaacctttg gttctgtatt gttttagtaa
     5881 tcgaaacatt gtttttgaat aactgctttc taattcattg aattgttgga gagaatagcg
     5941 caccc
//
