LOCUS       AP031272                6292 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE30 plasmid pNUITMV30_5 DNA, complete
            sequence.
ACCESSION   AP031272
VERSION     AP031272.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639559
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 346x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE30"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV30_5"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(42..575)
                     /locus_tag="NUITMVRE30_29760"
     CDS             complement(42..575)
                     /locus_tag="NUITMVRE30_29760"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFH06904.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(596..1546)
                     /locus_tag="NUITMVRE30_29770"
     CDS             complement(596..1546)
                     /locus_tag="NUITMVRE30_29770"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFH06905.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            complement(2429..3118)
                     /locus_tag="NUITMVRE30_29780"
     CDS             complement(2429..3118)
                     /locus_tag="NUITMVRE30_29780"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06906.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3363..4277)
                     /locus_tag="NUITMVRE30_29790"
     CDS             complement(3363..4277)
                     /locus_tag="NUITMVRE30_29790"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="BFH06907.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4259..4648)
                     /gene="mobC"
                     /locus_tag="NUITMVRE30_29800"
     CDS             complement(4259..4648)
                     /gene="mobC"
                     /locus_tag="NUITMVRE30_29800"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002498952.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="BFH06908.1"
                     /translation="MGLMSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     DDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC"
     gene            complement(4961..5158)
                     /locus_tag="NUITMVRE30_29810"
     CDS             complement(4961..5158)
                     /locus_tag="NUITMVRE30_29810"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06909.1"
                     /translation="MISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQLYK
                     SGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(5176..5709)
                     /locus_tag="NUITMVRE30_29820"
     CDS             complement(5176..5709)
                     /locus_tag="NUITMVRE30_29820"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06910.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     Typing          complement(597..1545)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3367..4277)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgaatagaa gattttttct tgtcatgaat gttccctcca gttatttcca aagttgccac
       61 catttttttt cagtatcaat ttgtttgtct attgtttctg ttgcttcaga aagagtcgtt
      121 gagtcattct ctggagcttc ttctgtataa gtcaactgta gtttttcttg cagtttttcg
      181 atttgtttgt tggcttgtag ggtgagttgt tgttgctggt cgagtagttt ttgtatatga
      241 gaattatttt gctctctgag ggcaagttgt tctcgtaaaa attcattttc ttcacgcaac
      301 tgatctagca acttgtttgt aaccacgttt ttgcttggta tattaacatt tttatcgttg
      361 tcttgttcgt actcgtgcaa cctatctagc aacatttggt tgcctaagtt gcgagcaagt
      421 tgcaatactc cagaacgcaa tacaacctta taaccacgct catttttagc tatcatggaa
      481 gaatctaatt tttggatatg tttcgatatt gcttgcctac tcatcttaaa aagttctgac
      541 aattccttta ttgtaaaggt ttcttgtggt tgcatactgg ttgcctccta ggttgctact
      601 ggaataaatt atctaaatca gataataaac tttttttctc ttttttcgaa agtgcggact
      661 ctgtttgttt ttcaagagct tctttctctt tttctagttg tttgatttgt tggaggagtt
      721 caagttctcg tttcttctgt ttgatttttt cgttaattgt tgtagcaggt tcttcgtatt
      781 ctttttgttt atcagctaaa gccaatgctt tattttgttc ttctttggtt ggtgcatgtt
      841 tattaaaatc atctgctttt ttgatttctg gtttaaacgt aaattgatag ccaacgacag
      901 gttttcctcg cccttttccg tatttctttt tgattgataa tcctttgaag attgcggtta
      961 attcctgtcg tataggcttt ataacacgat tatcaacgtt tgcttggttg ttccagtaac
     1021 tttttggaat atctaatagt tcatgaaaat cctctttaga aaaataagca aaacctttag
     1081 ttcgatattg ttttagtaat cgaaacatag tctttgcata gctactttgt aattcgttaa
     1141 attgttggag tgaatagcga acccactcat ctaaattgtt tagtaaaggt aaagcttttt
     1201 cgtaaacttg tatgtctaca tatggatctt tagcagttcc acgtatttta aactcactaa
     1261 acataacgaa tctatctata tcgagcccat tagcacttct tttaccaaaa cgtaaatcca
     1321 ttaatttatc ataagtcatt tctaaatcat taatgaaacg attattagca gttgctttat
     1381 aattacttaa atcttttagt tggtcaaaag taagacggac tgttttatcg cctttttcgc
     1441 gcattctaga cacaatagag aaaaatagat tcatttcaac tggtgtaaat ttccgcaacg
     1501 gaatggtatt taattcatga tggtatttca cgatttcatt tgccaaagat attcctcctt
     1561 actccttcaa tggatacaga cgttataaac aacgttttgt ctgtgataat gcatattata
     1621 acatacgtga gtgcaaaaaa agagtttaaa agcagacaaa atgtagttta aaagcagaca
     1681 aaatgtagtt taaaagcaga caaaatgtag tttaaaagca gacaaaatgt agtttataaa
     1741 acgctgtatc cattgaagga gtaaggacac agccgacggt aaagaggttt aaaaaaaggt
     1801 ttaaaaagag attataaaag agggtgacaa aatttcccgc ctaaaaacca cttctttttt
     1861 ctaccaacag gttcgcgcat ttttcaaaac tgccaattca aaaccttgct ccctacggtc
     1921 gctaaaaaag tgtgatcgct atgctctcac attgtactgc gcagtcgcct aaaggctcct
     1981 ttgctaaaaa caagtcagta acggaggtgg gggaagcctc tgatagctcg tctaagtcgt
     2041 tttaagacag tcttgggtaa aaaaccttat cttgttctta aagcgcctta caggcggtca
     2101 gggcaagccc taacaacccg aaaataaggg atacttaaaa acaccacaca gattttattt
     2161 tgtaaggtat aataacttgt cgcctatccg acagtcttgc gactggaagg aggtgtatct
     2221 aatgtttgaa cggttcattt gcccccttct tgtagaagtt tgtgtctctt tattcagtta
     2281 ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg cgacatcaac ctgaaaaaaa
     2341 gccccagaga atccgacctc tctggggctt tcgtgtatct aatgtttgat catttgcagg
     2401 ttcagtttaa catgattgct aatcaaattc aattttgttg ttgctgatcc aaccattcct
     2461 tgacactttc tagataattt aatgccgttt cttttttcgt tggattttgt atgtttgttg
     2521 cttgctgaag cagtgtttca taacgttcgt atgtgaccga ttgttgccgt tcttttcgtt
     2581 cttgttcact ggttgaaaaa gggattgtat cgctaaataa gttacaacaa gcttcttctg
     2641 tctgcgtatc tttaaaatat tgcacgtctt tttttacttg ctcatatttt tcttgtacct
     2701 ctttttgctg tttttctggt aagttttgat aaaaacgagt gagttttttc atgtttttat
     2761 ctgttaaacg tttctcttga tccacaaaaa tattgttcat gtcctcataa caagcggtaa
     2821 attcctccat tttttgtgct aatgcttgct cttttttctt tttatcacat gctgcttcat
     2881 acttttgagt gatatcccct aataaaatgc ttcttgaaac acgtttatta ccctctaaaa
     2941 cgccgaataa accacttaaa acgattgata aggcaaatat aaccccgacc aataaaaatc
     3001 gcttaaatcg aattttagag ccttttaatg aatatcttgt ttgataagcc ttatagctaa
     3061 gccaaataat tcctaaaata ctggcagaaa tgagtagata gggtaaatag aatgccatgt
     3121 ctgtttattc tccccatacc aaccaaaagg ctaataatgt tagtactaag caacataagg
     3181 gggcaagaat atccatcgat cgttgaatta aagccttttt ttgttcttgg tttcgctggt
     3241 ctaatagcgc ttggtggagt tttttgactt gtgaatccat cgtagtttgt atcctttctt
     3301 gggtataagg cgcaagaaag gcacgttgtc tctttatttc gttttcgatg tcttttaacg
     3361 ttttagagcg ataattcttg ttggtggtct gttcctcgtt caacgtgttg ttcatggttt
     3421 cttcgagtcg gtctaatttc cgattgttca ggcgtaaaag ttcgttgttc tcttcgtact
     3481 tcttctgcag tttcgcgaat gctagctcga gctcttcgta gctcgttggt tcgttgttga
     3541 ttgtaggttc gttctcgtct gtccagctca tgtaaaatcg tctccttttc gtaatccttt
     3601 cctagtttgt tggatcgtac ttttttcttt ttgtggacca attcgtaagt aaaatttttt
     3661 ccgcgattca agcaatgaat gccgaatgtt ttaagtgaat cacaaaattg ggtatacgtt
     3721 cgagtttttt tgtttttcat tgtttgatca atcgcaaacc gaatttcgtc tttccaaacc
     3781 ggttcatttt tttcttttaa tttaatttca gacatggttc gtttttcggt tttctttcct
     3841 cgttccgtga ttgttaagtc atgttcaaga caaatctcgt catttaggtc ataacatttg
     3901 tttaaaaatg cttgcccatg ggcttgaaat tttttcccag tttcaagatt aacggagtta
     3961 atgacaatgt gattatgaat atggtctttg tctgtatgtg tgtaaatcgc gatttggtgg
     4021 ttaggggcta tttttttcgc atactctgcc cctagttgat tggctttttc agcggtaatt
     4081 tctcctggtc gaaaggactg aatcaataag tgtgcttgta cacgatcgtc ttttccataa
     4141 agcgtgcgta tttgtttcat ttcacttttg gcataatcaa tgtcacagtt tattcctgtt
     4201 ttgacggttg ctcgtggttc tgcgtaattg attgctcggc tacatgaagt ggttcgactt
     4261 actttaactg ttgccatatc ttttgtagct ccttttgcat gatttttagg ttttcggata
     4321 aacgttgagc atcatcagga gcaacgtcgt gttggtgtgt attgcaccac ttggcaattt
     4381 gattgatatt ccctccagct ttggctaaat cgcgcgcgat tgcctgggca atgtctggcg
     4441 ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc tttcacgaaa gccggcacac
     4501 tcatttgcaa agcttcagct gactgcttca gctttgcaaa ttcggattcg ctcactcgaa
     4561 aactgatttg ttttggctcg gatcgatagg tatatttttt agaatgatcg ctagccaaat
     4621 tttgatgttg ttcgctcata agtcccaccc tttcagtacg tcctacggac gtatcgttcg
     4681 caataccatt gtacctaaaa aggtacaatg agtaaagctc actaaaaggg tttggcaagc
     4741 tctatatttg ctagccaaat acctccgtat ttggtgcaaa taaccgcttg tcaaaggagc
     4801 gcaagctccc ccttgaatcc ccctcaaaat ccacgctcca caatacaaaa aaaggctagc
     4861 atttgctagc cactttttgt aaagtttcgc ttatttcacc gaaggtgaaa ttgttgcttt
     4921 gtaatattca aacaacccta ttcttctaaa atgtatcgat ttattttttt ttagacttta
     4981 ttatgtctaa gacaactaag attaacacta aaaataaacc agatttgtat aattgccctc
     5041 cgcttagcat attaacaatt atagcaacag ctatagcaaa tataaaaata cgaatagtag
     5101 ctatttgcgg attcttttta gtaaatgttt ttaatgattt taatgattta gatatcaaaa
     5161 acatcactcc ttaacttata acgttgaata tctagtagct acaactcttc cagctgcatc
     5221 atatcccctt tggtaataaa atccaatcca gccattacca tttttgtccc attcttttgc
     5281 aactgcattc cctggagaag cacctttttt agtccaactt gcacctgtta acaaagcacc
     5341 aattgcccaa cctgggatac caaacataga agtgctagct ttccctcctg cccaaaaggc
     5401 agcattaata gcattagcag ctactccagt ggttacagca atatttgtat attccttttt
     5461 gccccatctt gcaactgcag ctcttgtcat gatatctgaa gaatttgcaa aatcttccgc
     5521 actataacca tcctcatatt tagttacgac cataccatct tcagtgtact caataactac
     5581 attttcagta gcattgtttt cgtttaatga taccacttgg gaattttctg aagcataaac
     5641 tatagtagtt gacggtaaaa caactgcccc aatagttaac aaagctaccc ctaaagtaaa
     5701 ttttttcata gaatttcctc ctgatacaga aaaatatata gaaaacgttt tctttgtgaa
     5761 aatcataaaa caaaaggtat aggcttgtca atattttatt tagtaaagat aatttatatt
     5821 tttgataatg tggtatagtt agaatgcatt aggaaaatat gttatattaa attcttgttg
     5881 acataatgtc gagtatgcca agtagccctt gtatcaaccg atacaggggc tcactttgtg
     5941 atttttcatt ggtctaggta aaaagctctt tttaaggctt gattttctgc agttgcttct
     6001 ttccgtttct ttgtgaattt ttaccagtca ttttttactg ggttgttatt tttaataaaa
     6061 tatgataaat ttttggtagc gcttcataag cgcgttgctt tttcaagaat aaacactcat
     6121 tattttttaa ctggctgctc gaaagagcag tcttttttat tatttatctg tttcttttac
     6181 aaacaattct tcgttaggac tgttgtaata atttggattc aatttcaatt caccatgttt
     6241 attcccttta ttgatcactg acaataacat cggactgaat cctagaattc ct
//
