LOCUS       AP031271               12317 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE30 plasmid pNUITMV30_4 DNA, complete
            sequence.
ACCESSION   AP031271
VERSION     AP031271.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639559
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12317)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 346x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..12317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE30"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV30_4"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            399..1814
                     /locus_tag="NUITMVRE30_29620"
     CDS             399..1814
                     /locus_tag="NUITMVRE30_29620"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06890.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGTL
                     DDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDEVSP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENEREK
                     ALVASERAKIEEDKKSVFDLKDELDAVFQKDPVPLTSFEKLPDGSYRLSSDDFIDLYQK
                     ASNATKSKKYADMADEYLNTSVEENMALFDRNLALEEENVFLKKQQKVDVSAIQAENKK
                     LKEENRSLRQRLEKLKNAFQKSITRLSLRFGLLEKEMDIPDELEILGREKELSSQENAP
                     QRFLGPSEDEIEW"
                     /name="hypothetical protein"
     gene            2285..3259
                     /locus_tag="NUITMVRE30_29630"
     CDS             2285..3259
                     /locus_tag="NUITMVRE30_29630"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06891.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNVHNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDRYEENIAYIEILKFHD
                     GKGRIDFNPSKINQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEGAE"
                     /name="hypothetical protein"
     gene            3256..3675
                     /locus_tag="NUITMVRE30_29640"
     CDS             3256..3675
                     /locus_tag="NUITMVRE30_29640"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06892.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGIIM
                     LDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            3684..3992
                     /locus_tag="NUITMVRE30_29650"
     CDS             3684..3992
                     /locus_tag="NUITMVRE30_29650"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06893.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHRHLAQTTDEAEKEFWLKLMSHVMQLNQISVINSKEKGM"
                     /name="hypothetical protein"
     gene            3996..4358
                     /locus_tag="NUITMVRE30_29660"
     CDS             3996..4358
                     /locus_tag="NUITMVRE30_29660"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06894.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFDDFERWLSSISDDQQHEKSVDNEWLSDEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            4579..5994
                     /locus_tag="NUITMVRE30_29670"
     CDS             4579..5994
                     /locus_tag="NUITMVRE30_29670"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06895.1"
                     /translation="MGFSISFKKGTKNTSISHNNRVLTDSQKINDWHKHIDFSKSDENI
                     YLEQTDIREKYEELFGESVEEYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQVGTF
                     DDFQTTRADGSSTGISEQQAEQNRVIANKILVQYYKEFQERNPNLAIYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLQVDKENGRVLWNNFRGQEIESVERLMA
                     DFGWNRQLVGTNKIKDIHEYKEIMSEISELRQTALKERESVAEEMKYISKQREENEREK
                     ALVASERAKIEEDKKSVFDLKDELDAVFQKDPVPLTSFEKLPDGSYRLSSDDFIDLYQK
                     ASNATKSKKYADMADEYLNTSVEENMALFDRNLALEEENVFLKKQQKVDVSAIQAENKK
                     LKEENRSLRQRLEKLKNAFQKSITRLSLRFGLLEKEMDIPDELEILGREKELSSQENAP
                     QRFLGPSEDEIEW"
                     /name="hypothetical protein"
     gene            6465..7439
                     /locus_tag="NUITMVRE30_29680"
     CDS             6465..7439
                     /locus_tag="NUITMVRE30_29680"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06896.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNVHNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDRYEENIAYIEILKFHD
                     GKGRIDFNPSKINQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="hypothetical protein"
     gene            7436..7855
                     /locus_tag="NUITMVRE30_29690"
     CDS             7436..7855
                     /locus_tag="NUITMVRE30_29690"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06897.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGIIM
                     LDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            7864..8172
                     /locus_tag="NUITMVRE30_29700"
     CDS             7864..8172
                     /locus_tag="NUITMVRE30_29700"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06898.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            8176..8538
                     /locus_tag="NUITMVRE30_29710"
     CDS             8176..8538
                     /locus_tag="NUITMVRE30_29710"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06899.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            8759..10174
                     /locus_tag="NUITMVRE30_29720"
     CDS             8759..10174
                     /locus_tag="NUITMVRE30_29720"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06900.1"
                     /translation="MGFSISFKKGTKNTSISHNNRVLTDSQKINDWHKHIDFSKSDENI
                     YLEQTDIREKYEELFGESVEEYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQVGTF
                     DDFQTTRADGSSTGISEQQAEQNRVIANKILVQYYKEFQERNPNLAIYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLQVDKENGRVLWNNFRGQEIESVERLMA
                     DFGWNRQLVGTNKIKDIHEYKEIMSEISELRQTALKERESVAEEMKYISKQREENEREK
                     ALVASERAKIEEDKKSVFDLKDELDAVFQKDPVPLTSFEKLPDGSYRLSSDDFIDLYQK
                     ASNATKSKKYADMADEYLNTSVEENMALFDRNLALEEENVFLKKQQKVDVSAIQAENKK
                     LKEENRSLRQRLEKLKNAFQKSITRLSLRFGLLEKEMDIPDELEILGREKELSSQENAP
                     QRFLGPSEDEIEW"
                     /name="hypothetical protein"
     gene            10571..11482
                     /locus_tag="NUITMVRE30_29730"
     CDS             10571..11482
                     /locus_tag="NUITMVRE30_29730"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /note="frameshifted, insertion/deletion at around 11437"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="BFH06901.1"
                     /translation="MKILSRSLFFFEEEQNLSKRGTIVNEWEKLNRISNRRLKYQSLTL
                     PLKMQWSIDRITIVGKLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLD
                     QHEENIAYIEILKFHEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIACDIV
                     DIPDDFVSQYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVP
                     PEITTWWRLELQLRRGKATDWHEMVHESLDSFASPHFLPLDVKTNDKVMIFGLIANPEM
                     WGFLERRMKVQVERYFEKRKSK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            11448..11594
                     /locus_tag="NUITMVRE30_29740"
     CDS             11448..11594
                     /locus_tag="NUITMVRE30_29740"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06902.1"
                     /translation="MRDILKRESQNDELTNHLRESFAESADDLKRELDTWLQGLDVTEE
                     GAE"
                     /name="hypothetical protein"
     gene            11591..12010
                     /locus_tag="NUITMVRE30_29750"
     CDS             11591..12010
                     /locus_tag="NUITMVRE30_29750"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH06903.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGIIM
                     LDKELIGVVRSIYRSKSDQLTGHLSVNNTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     Typing          2369..3251
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.191"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          6549..7431
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.981"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          10641..11591
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.743"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          382..1811
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.648"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4565..5991
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.353"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8745..10171
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.353"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgatcagcaa cacgaaaaat cagtggataa tgagtggctt tctgatgaaa aatcagcgcc
       61 aagcgaacga gattatcccg atgaatctga cggcttattc ggagtgatac cgaataagaa
      121 aagtcagatg agtgagagta aatttacgtc aatgtttaat gacgtgaatt tacgataagc
      181 gattattttg cgtgaagcaa aataaagcgt aaaaggagtt gagagcgtga tacgctttcg
      241 gctccttttt tcgttgtcac ttgtgacagc gaaaaccctc ggagagcgtt tggaattgtg
      301 acatgaatgt cacaattagg aaaacgttac tctttgacaa agagtaacag aaaggtttac
      361 actcttttta ttagcgacag ccgaaggagg gttttaactt ggggttcagt atttcgttca
      421 aaaagggaac aaagaataca agcatttctc ataataatcg tgagttaact gatagccaaa
      481 aaatcaatga ctggcataag catattgatt tcacaaaatc ggatgaaaat atctatttag
      541 aacaaacaaa tattcgtgaa aaatatgaag aattattcgg tgaagccgta gaaaaatata
      601 atgcaaagca gaaaagagcc gatagaaaaa tcgaggacta tttagcaaag gttcgcaagg
      661 ataaaaaatt ggaaccacag cgagaattta tcgtgcaaat tggtactctt gatgattttc
      721 gtactactcg tgatgatggt tcttctactg gcatatcgga acaacaagca gaacaaaatc
      781 gagtgattgc caacaaaatt ttagttgagt atttcaaaga atttaaagaa cggaatccta
      841 atttatccgt ctacaatgct gttattcata atgacgaagt aagtccgcac ctgcatttga
      901 atattgttcc tgttgctgaa gggtataaac ggggtgttca aaagcaacca agttttaaca
      961 aagccttgca acagcagggt ttacgagcag ataaagagaa tggtcgagtg atttggaata
     1021 attttaggaa tcaggaagtt gagtcaattg aacgtttgat ggcagatttt ggttgggagc
     1081 gtgagttagt cggtactaac aaaatcaaag atgttcacga atataaagaa atcatgagcg
     1141 agatttcaga attgcgtcaa acggccgtaa aagagcgtga gagcgtttct gatgagttga
     1201 ggtatatttc taggcaaaga gaagaaaatg agcgagaaaa ggctctggtg gcttctgaga
     1261 gagctaaaat tgaagaggat aagaaatcag tttttgattt gaaagatgag ctagatgctg
     1321 ttttccaaaa agaccctgtt cctttaacga gttttgaaaa attaccggat ggctcgtatc
     1381 gtttgtctag tgatgatttt attgatctct atcaaaaagc atctaatgcc actaaaagca
     1441 aaaaatacgc tgatatggct gacgaatatc ttaacacctc ggtggaggaa aacatggcac
     1501 tgtttgatag aaatctagcg ttggaagaag aaaatgtttt tctcaaaaaa cagcaaaagg
     1561 ttgatgtttc agcaattcaa gcggaaaaca aaaagctaaa agaagagaat cgttctttgc
     1621 gccagcgatt agaaaaattg aaaaacgcct ttcaaaaatc tatcactcga ttgtctttac
     1681 gctttgggct gttagaaaaa gaaatggata ttccggatga gttagaaatt ttaggcagag
     1741 agaaagagct atcaagccag gagaatgctc cacaacgctt cttaggacct tctgaagatg
     1801 aaattgagtg gtagttatac ggtgcagtca acgataccgg aggcggtctg tgccgaggat
     1861 aaggagttga cggctaaacc actaatccct gaagctgaca tttgctaaaa aatggcaagt
     1921 ccattttttg gccaacctag cgttagcgaa agtttggggg cgcccttttc aaagggcgcg
     1981 gtcttatgtt tttgtctatc gaataaaaat attcctgtgt atttgcatag taaaaaagaa
     2041 gtgctattat tgagttatca aacaaatgaa ctgaaaaaag agcaaaaaaa tagtcgagca
     2101 acgctaccaa cgttctcgac tcttacggaa actatctcta ctagtcccgt aactcaaatt
     2161 cattatacga atatttttag gtttcttcaa gactagagat tgttggttat gaatttttta
     2221 gagttacgtg aactctgaaa aattctttcc accaatctct tttttcttca aggaggaaca
     2281 aaatttgagt aaacgtggca cgatcataaa tgagtgggaa aaacttaacc gcatatctaa
     2341 caggcggtta aatgtacata atatgtacac ccccttaaaa atgcagtggt caattgaccg
     2401 aattaccatt gttggaaaac tgaaggaaaa tatttattat catactcaaa atgatgtgtt
     2461 gattttagat tttgaacagc ttatgcgcct aaacgaaggt aacggatatt taaaagcggt
     2521 tgggaataat ggctggcaat tattagaccg atatgaagaa aatattgctt atattgagat
     2581 tcttaagttt catgatggaa aagggcggat tgactttaac cccagtaaaa tcaatcaatt
     2641 tttagctggt tcaatgaaaa atttcattca tgatttattt ttagaaccac atttctcacg
     2701 tgcagatatt gcttgcgata ttatcgatgt gcctgatgag tttattacac aatatcgagt
     2761 agttgaccct gtttctttca agccaattta tgggcggtct gggaaactgg aaacggctta
     2821 ttggggaagt cgtgccagcg agcgacaaat aaggatgtac aacaaaaaat tggaacagga
     2881 aaccaagcgg aaaattgtac caccagaaat aaaaacttgg tggcgactgg aattgcagtt
     2941 aaggcgtggg aaagcgacgg attggcatgc gatggttcac gaaagtctag atagttttgc
     3001 tagccctcat tttctacctc ttgatgtaaa aacaaatgat aaggtcatga tttttggatt
     3061 gattgctaat cctgaaatgt ggggtttttt agagcgtaga atgaaataca agttgagaga
     3121 tattttgaaa agagaaagtc aaaatgatga gctgacgaat catttacgag aaacattttc
     3181 agaatcggca gatgttttga aaaaagaatt agatacttgg ttacaagggc ttgatgtgac
     3241 ggaagaaggt gctgaatgag taatccagta gaaaaaatga ccataaaaaa tcttgctgat
     3301 gaattgggtg tatcgaaaaa tacagtgcgc aatcaactgg tcaattcagg gtttgacctt
     3361 gctcagtata aagaaaatgg cattatcatg ctagataaag agttgattgg tgttgttcgg
     3421 tcaatttatc ggtcaaaaag tgatcaattg actggtcaat taccggtcaa taacacaggc
     3481 agtgattttc ctttagaaaa agagaatgag tttttgcgtg atcaattagc tgagaaggac
     3541 aaacatattt ctgaattaac agaaatgatt cgtgatcaac aaaaattgac cctgcttcag
     3601 caacaaaaaa acgatcagtt gttactagaa aatcaagaat taaaaaatcg gaaatggtgg
     3661 caattctgga agtgagatgt gacatggata aaattgattt gagagaagaa acagtcaata
     3721 gaattgaagt gatgtatgaa cggttagctg attatttgaa tctttcggat gaagataatt
     3781 atagtgagat gcttgaacga atgatggatg ttctcaactc ggttatcaat gctgatctag
     3841 caaaatcttc agttttgact aatgaatttg ttcataggca tttagcgcaa acaactgatg
     3901 aagctgaaaa agaattttgg ttgaaattaa tgagtcatgt tatgcagtta aaccaaattt
     3961 ccgttattaa ttccaaagaa aaggggatgt agtttatggg gatgacttta gcagatatta
     4021 aagcacaaga agaaaaattg aaaaaagaac aggcaaaact tcgtcaacag aaaaaacgat
     4081 tgattgagaa ggaaaatgct gaaatgggta aggcacttcg gaagcatttt ggatttgaat
     4141 cttttgatga ttttgagcgt tggctatcgt caatttctga cgatcagcaa cacgaaaaat
     4201 cagtggataa tgagtggctt tctgatgaaa aatcagcgcc aagcgaacga gattatcccg
     4261 atgaatctga cggcttattc ggagtgatac cgaataagaa aagtcagatg agtgagagta
     4321 aatttacgtc aatatttaat gacgtgaatt tacgataagc gattattttg cgtgaagcaa
     4381 aataaagcgt aaaaggagtt gagagcgtga cacgctttcg gctccttttt tcgttgtcac
     4441 ttgtgacagc gaaaaccctc ggagagcgtt tggaattgtg acatgaatgt cacaattagg
     4501 aaaacgttac tctttgacaa agagtaacag aaaggtttac actcttttta ttagcgacag
     4561 cgcaaaggag ggtttgaatt ggggtttagt atatcgttca aaaaagggac aaagaacacg
     4621 agcatttctc acaataatcg tgtgttaaca gacagccaga aaattaatga ttggcataag
     4681 catattgatt tctcaaagtc ggatgaaaat atctatttgg aacaaacgga tattagagaa
     4741 aagtatgaag aattatttgg tgaatcggta gaagaatata atgcaaaaca aaaaagagct
     4801 gatagaaaaa ttgaggacta tttagccaaa gttcgcaagg ataaaaagtt ggaacctcaa
     4861 cgagaattta ttgtgcaagt gggaacattt gatgattttc agaccactag ggcagatggt
     4921 tcttctactg gcatatcgga acaacaagct gaacaaaatc gagtaattgc caataaaatt
     4981 ttggttcagt attacaagga atttcaagag agaaacccaa atttggctat ttataatgct
     5041 gttattcata atgacgaaat aagtccgcac ctgcacttga atattgtacc tgttgctgaa
     5101 ggttacaaac gtggtgttca gaagcaacca agctttaata aagccttgca acagcagggc
     5161 ttacaggtag ataaagaaaa tggccgagtg ctctggaata attttcgtgg tcaagaaatt
     5221 gaatcggttg agcgcttaat ggccgatttt ggttggaatc gtcagttagt tggtaccaat
     5281 aaaattaagg atattcatga atacaaagaa attatgagcg aaatttccga attgcgtcaa
     5341 acggctttaa aagagcgtga gagcgttgct gaggaaatga agtatatttc taagcaaaga
     5401 gaagaaaatg agcgtgagaa agctctggta gcttctgaga gagctaaaat tgaagaggat
     5461 aagaaatcag tttttgattt gaaagatgag ctagatgctg ttttccaaaa agaccctgtt
     5521 cctttaacga gttttgaaaa attaccggat ggctcgtatc gtttgtctag tgatgatttt
     5581 attgatctct atcaaaaagc atctaatgcc actaaaagca aaaaatacgc tgatatggct
     5641 gacgaatatc ttaacacctc ggtggaggaa aacatggcac tgtttgatag aaatctagcg
     5701 ttggaagaag aaaatgtttt tctcaaaaaa cagcaaaagg ttgatgtttc agcaattcaa
     5761 gcggaaaaca aaaagctaaa agaagagaat cgttctttgc gccagcgatt agaaaaattg
     5821 aaaaacgcct ttcaaaaatc tatcactcga ttgtctttac gctttgggct gttagaaaaa
     5881 gaaatggata ttccggatga gttagaaatt ttaggcagag agaaagagct atcaagccag
     5941 gagaatgctc cacaacgctt cttaggacct tctgaagatg aaattgagtg gtagttatac
     6001 ggtgcagtca acgataccgg aggcggtctg tgccgaggat aaggagttga cggctaaacc
     6061 actaatccct gaagctgaca tttgctaaaa aatggcaagt ccattttttg gccaacctag
     6121 cgttagcgaa agtttggggg cgcccttttc aaagggcgcg gtcttatgtt tttgtctatc
     6181 gaataaaaat attcctgtgt atttgcatag taaaaaagaa gtgctattat tgagttatca
     6241 aacaaatgaa ctgaaaaaag agcaaaaaaa tagtcgagca acgctaccaa cgttctcgac
     6301 tcttacggaa actatctcta ctagtcccgt aactcaaatt cattatacga atatttttag
     6361 gtttcttcaa gactagagat tgttggttat gaatttttta gagttacgtg aactctgaaa
     6421 aattctttcc accaatctct tttttcttca aggaggaaca aaatttgagt aaacgtggca
     6481 cgatcataaa tgagtgggaa aaacttaacc gcatatctaa caggcggtta aatgtacata
     6541 atatgtacac ccccttaaaa atgcagtggt caattgaccg aattaccatt gttggaaaac
     6601 tgaaggaaaa tatttattat catactcaaa atgatgtgtt gattttagat tttgaacagc
     6661 ttatgcgcct aaacgaaggt aacggatatt taaaagcggt tgggaataat ggctggcaat
     6721 tattagaccg atatgaagaa aatattgctt atattgagat tcttaagttt catgatggaa
     6781 aagggcggat tgactttaac cccagtaaaa tcaatcaatt tttagctggt tcaatgaaaa
     6841 atttcattca tgatttattt ttagaaccac atttctcacg tgcagatatt gcttgcgata
     6901 ttatcgatgt gcctgatgag tttattacac aatatcgagt agttgaccct gtttctttca
     6961 agccaattta tgggcggtct gggaaactgg aaacggctta ttggggaagt cgtgccagcg
     7021 agcgacaaat aaggatgtac aacaaaaaat tggaacagga aaccaagcgg aaaattgtac
     7081 caccagaaat aaaaacttgg tggcgactgg aattgcagtt aaggcgtggg aaagcgacgg
     7141 attggcatgc gatggttcac gaaagtctag atagttttgc tagccctcat tttctacctc
     7201 ttgatgtaaa aacaaatgat aaggtcatga tttttggatt gattgctaat cctgaaatgt
     7261 ggggtttttt agagcgtaga atgaaataca agttgagaga tattttgaaa agagaaagtc
     7321 aaaatgatga gctgacgaat catttacgag aaacattttc agaatcggca gacgatttga
     7381 aacgagaatt agatacatgg ctccaaggac ttgatgtaac ggaagtaggt gctgaatgag
     7441 taatccagta gaaaaaatga ccataaaaaa tcttgctgac gaattgggtg tatcgaaaaa
     7501 tacagtgcgc aatcaactgg tcaattcagg gtttgacctt gctcagtata aagaaaatgg
     7561 cattatcatg ctagataaag agttgattgg tgttgttcgg tcaatttatc ggtcaaaaag
     7621 tgatcaattg actggtcaat taccggtcaa taacacaggc agtgattttc ctttagaaaa
     7681 agagaatgag tttttgcgtg atcaattagc tgagaaggac aaacatattt ctgaattaac
     7741 agaaatgatt cgtgatcaac aaaaattgac cctgcttcag caacaaaaaa acgatcagtt
     7801 gttactagaa aatcaagaat taaaaaatcg aaaatggtgg caattctgga agtgaggtgt
     7861 gacatggata aaattgattt gagagaagaa acagtcgata gaattgaatt gatgtatgaa
     7921 cggttagctg attatttgaa tttgccggat gaagataatt atagtgaaat gcttgaacga
     7981 attatggatg ttctcaactc ggttatcaat gctgatttag caaaatcttc agttttgact
     8041 aatgaatttg ttcataagca tttagcgcaa acaactgatg aatcagaaaa agaattttgg
     8101 ttgaaattaa tgagtcatgt tatgcagtta aaccaaattt ccgttattaa ttccaaagaa
     8161 aaggagatgt agtttatggg aatgacttta gcagatatta aagcgcaaga agaaaaatta
     8221 aaaaaagaac aagcgaaact tcgccaacag aaaaaacgct tgattgagaa agaaaatgct
     8281 gcaatgggca aggctcttcg caagcgtttt gggtttgaaa cttttgctga ttttgagcgg
     8341 tggctatcgt cgatttctga cgatcagcaa cacgaaaaat cagtggataa tgagtggctt
     8401 gctgatgaaa aatcagcgcc aagcgaacga gattatcccg atgaatctga cggcttattc
     8461 ggagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatatttaat
     8521 gacgtgaatt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
     8581 gagagcgtga tacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaaaaccctc
     8641 ggagagcgtt tggaattgtg acatgaatgt cacaattagg aaaacgttac tctttgacaa
     8701 agagtaacag aaaggtttac actcttttta ttagcgacag cgcaaaggag ggtttgaatt
     8761 ggggtttagt atatcgttca aaaaagggac aaagaacacg agcatttctc acaataatcg
     8821 tgtgttaaca gacagccaga aaattaatga ttggcataag catattgatt tctcaaagtc
     8881 ggatgaaaat atctatttgg aacaaacgga tattagagaa aagtatgaag aattatttgg
     8941 tgaatcggta gaagaatata atgcaaaaca aaaaagagct gatagaaaaa ttgaggacta
     9001 tttagccaaa gttcgcaagg ataaaaagtt ggaacctcaa cgagaattta ttgtgcaagt
     9061 gggaacattt gatgattttc agaccactag ggcagatggt tcttctactg gcatatcgga
     9121 acaacaagct gaacaaaatc gagtaattgc caataaaatt ttggttcagt attacaagga
     9181 atttcaagag agaaacccaa atttggctat ttataatgct gttattcata atgacgaaat
     9241 aagtccgcac ctgcacttga atattgtacc tgttgctgaa ggttacaaac gtggtgttca
     9301 gaagcaacca agctttaata aagccttgca acagcagggc ttacaggtag ataaagaaaa
     9361 tggccgagtg ctctggaata attttcgtgg tcaagaaatt gaatcggttg agcgcttaat
     9421 ggccgatttt ggttggaatc gtcagttagt tggtaccaat aaaattaagg atattcatga
     9481 atacaaagaa attatgagcg aaatttccga attgcgtcaa acggctttaa aagagcgtga
     9541 gagcgttgct gaggaaatga agtatatttc taagcaaaga gaagaaaatg agcgtgagaa
     9601 agctctggta gcttctgaga gagctaaaat tgaagaggat aagaaatcag tttttgattt
     9661 gaaagatgag ctagatgctg ttttccaaaa agaccctgtt cctttaacga gttttgaaaa
     9721 attaccggat ggctcgtatc gtttgtctag tgatgatttt attgatctct atcaaaaagc
     9781 atctaatgcc actaaaagca aaaaatacgc tgatatggct gacgaatatc ttaacacctc
     9841 ggtggaggaa aacatggcac tgtttgatag aaatctagcg ttggaagaag aaaatgtttt
     9901 tctcaaaaaa cagcaaaagg ttgatgtttc agcaattcaa gcggaaaaca aaaagctaaa
     9961 agaagagaat cgttctttgc gccagcgatt agaaaaattg aaaaacgcct ttcaaaaatc
    10021 tatcactcga ttgtctttac gctttgggct gttagaaaaa gaaatggata ttccggatga
    10081 gttagaaatt ttaggcagag agaaagagct atcaagccag gagaatgctc cacaacgctt
    10141 cttaggacct tctgaagatg aaattgagtg gtagttacgg tgcagtcaac ggtaccggag
    10201 gcggtttgtg ccgaggataa ggagttgacg gctaaaccac tagcctatta agctgacatt
    10261 tgctaaaaaa tggccagtcc attttttggc caacctagcg ttagcgaagt ttgggggcgc
    10321 ccttttcaaa gggtgcggtt ttattttttg gatatttgca tagctaaaga gaagtgctat
    10381 tattgagtta gcaaacaaat gaactgaaaa aagagtaaaa aaatagtcga gcaacgctac
    10441 caacgttctc gacctttacg gaaactaatc gcactagctc cgtaacttct taatcattat
    10501 acgaatattt ttagatttct tcaagactag agattttttg gtaatgaatt tttaagagtt
    10561 acgtgaactt ttgaaaattc tttccagaag tctctttttc tttgaggagg aacaaaattt
    10621 gagtaaacgt ggcacgatcg tgaatgagtg ggaaaaactt aaccgcattt ctaacaggcg
    10681 gttaaagtat caaagtttga cccttccctt aaaaatgcag tggtcaattg accgaattac
    10741 catagtcgga aaactgaaag aaaatattta ttatcacact ccaaatgatg tgttgatttt
    10801 aaattttgaa cagcttatgc gtttgaacga aggcaatggt tatttaaaag ctgttgggaa
    10861 taatgggtgg caattattag accaacacga ggaaaatata gcttatattg agattcttaa
    10921 atttcatgaa ggaaaagggc ggattgactt taatcctaat aaaatcaatc aatttttatc
    10981 aggttccatg aaaaatttca ttcatgattt atttctagaa cctcattttt cacgtgcgga
    11041 tattgcttgc gatattgtag atattcccga tgactttgtt agtcagtatc gtgtagttga
    11101 tcctgtgtct tttaaaccaa tttatgggcg ttctggtaag ttagaaacgg catattgggg
    11161 tagtcgtgcc agtgaacgac aaattcgaat gtacaataaa aaattggaac aagaaagaaa
    11221 acgtaaaatt gttccgccgg aaataacgac atggtggcga ctagaattgc agttacgaag
    11281 gggaaaagca actgattggc atgagatggt tcatgaaagt ttggatagct ttgctagccc
    11341 tcattttcta cctcttgatg taaaaacaaa tgataaggtc atgatttttg gattgattgc
    11401 taatcctgaa atgtggggtt ttttagagcg tagaatgaaa gtacaagttg agagatattt
    11461 tgaaaagaga aagtcaaaat gatgaattga cgaatcattt gcgagagtca tttgcggaat
    11521 cggcagacga tttgaaacga gaattagata catggctcca aggacttgat gtaacggaag
    11581 aaggtgctga atgagtaatc cagtagaaaa aatgaccata aaaaatcttg ctgatgaatt
    11641 gggtgtatcg aaaaatacag tgcgcaatca actggtcaat tcagggtttg accttgctca
    11701 gtataaagaa aatggcatta tcatgctaga taaagagttg attggtgttg ttcggtcaat
    11761 ttatcggtca aaaagtgatc aattgactgg tcatttatcg gtcaataaca cgggcagtga
    11821 ttttccttta gaaaaagaga atgagttttt gcgtgatcaa ttagctgaga aggacaaaca
    11881 tatttctgaa ttaacagaaa tgattcgtga tcaacaaaaa ttgaccctgc ttcagcaaca
    11941 aaaaaacgat cagttgttac tagaaaatca agaattaaaa aatcggaaat ggtggcaatt
    12001 ctggaagtga gatgtgacat ggataaaatt gatttgagag aagaaacagt caatagaatt
    12061 gaagtgatgt atgaacggtt agctgattat ttgaatcttt cggatgaaga taattatagt
    12121 gaaatgcttg aacgaatgat ggatgttctc aactcggtta tcaatgctga tttagcaaaa
    12181 tcttcagttt tgactaatga atttgttcat aagcatttag cgcaaacaac tgatgaagct
    12241 gaaaaagaat tttggttgaa attaatgagt catgttatgc agttaaacca aatttccgtt
    12301 attaattcca aagaaaa
//
