LOCUS       AP031266                2047 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE19 plasmid pNUITMV19_10 DNA, complete
            sequence.
ACCESSION   AP031266
VERSION     AP031266.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639548
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE19
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 281x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE19"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV19_10"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            29..250
                     /locus_tag="NUITMVRE19_31340"
     CDS             29..250
                     /locus_tag="NUITMVRE19_31340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03925.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            251..508
                     /locus_tag="NUITMVRE19_31350"
     CDS             251..508
                     /locus_tag="NUITMVRE19_31350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03926.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(644..1246)
                     /locus_tag="NUITMVRE19_31360"
     CDS             complement(644..1246)
                     /locus_tag="NUITMVRE19_31360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002919940.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="BFH03927.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1313..1468)
                     /locus_tag="NUITMVRE19_31370"
     CDS             complement(1313..1468)
                     /locus_tag="NUITMVRE19_31370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03928.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="hypothetical protein"
     gene            complement(1565..1723)
                     /locus_tag="NUITMVRE19_31380"
     CDS             complement(1565..1723)
                     /locus_tag="NUITMVRE19_31380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03929.1"
                     /translation="MFFGFGVYELGGGGGDYDTPLIGHWSLVDSCLALLSQGFQRFFLT
                     KSVMPRV"
                     /name="hypothetical protein"
     Typing          411..586
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(717..1246)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 agacggacgt taaatggaag gagcataaat gagagtgata aaatcagaaa aattcacttt
       61 ggccgaaatt atgacgacca gcgaacttgc acaagaactt gattacaacc aagcttacgt
      121 tttgcgctta gcgaaagaac atctgactga aggaaaagaa tatcgctctg ccggtagaag
      181 aaactatctt ttcagcccag aagcccttaa aaaactagaa aaagtcataa ccaccaaaga
      241 aggtgagtaa atgcctaaag aaacaactat caacgttgaa caactagaaa aattgctaga
      301 atcatattcc aatgatttta tcgaattgac cggcggaaaa gtcgaagaaa ccaccgacct
      361 ctttgaccta cttctcacaa atattctgga attaaactct gaagaacaaa aagagcataa
      421 gggaaaactg atagcacctt cctacaggaa ggtggctaag tcggctgtgc caactggttt
      481 tcttacaaaa caaccttata ttttttgata tcacaaaaaa atccattttg gacctatttt
      541 cggtcataat atagtaccta cttttggtca tagtttcgtc cgtagttttg gtcatagtga
      601 ctaagacttt cacgcacaaa aaaagagctg aaattaacag ctcttatcct ttatttattt
      661 ttgaatttac acttccacca tgacgattcg attttatata ttgcccgata atataagctg
      721 aattatcaca aagaagcgga aaccaatcat caaatctttc tgacattgca tagtcaatta
      781 aatctttgat ttcagtgata ttcttagaat caacaaacga catcatctct ctaatcaact
      841 catatcgttc tgcactcgtt actgacaaat aactagcaat ttctgcacca ccatgagcga
      901 taatatcact tttagcatat tgaaattttt ctggattatc catatgagca aaatatcgca
      961 ccataccttt tgcattagca catttttgcg ggtttggaga ccgaagcttt tgagttatct
     1021 cacgtatttg atcatacgtc tttttaccgc taaacattat caaaacatgc caatgaggct
     1081 ttttcaattc accgtcagga ttcacatctt tatcatgaag tggactctca acccaaggaa
     1141 catgtaactc atctaaaaac tccctccaat tttcaggagc cgattcagga tagactacaa
     1201 acgtccaatt ccttgtgcgt tcatctttag tttttctact cgtcatgcta taattacctt
     1261 aattgaacaa cagtctaaca gcaaaagcag tgcgccaaca ctaatgcttt tgctattttt
     1321 tttgctcatt ttcctttccg ttagcattaa cccaaattgt aataagacca gacttactaa
     1381 gacccatttg tttcgcaatt ttatcaagat tatctgccac ttccttagta agcgttacaa
     1441 cgactctctt tttttcatta ctgatcataa ttcgtatcct ccaacgactt tttcttaaaa
     1501 accatagtaa caaaaaagta actaaggtgc aacagtttag atactaatta gcgaataata
     1561 ggccttaaac ccttggcatt actgattttg tcaaaaaaaa acgctgaaac ccttgggaga
     1621 gtaaggctaa acagctatcg accaatgacc aatgaccaat aaggggggtg tcgtagtccc
     1681 cccctcctcc gcccaattca taaacaccaa aaccaaaaaa caaacctcag ggctccgccc
     1741 cgaaccccga cctccttcta ccgaaatata tgcaggcgcc acgcttacta tttcgttcag
     1801 ctatcggctt ttgcaatcta acacgaccgt ttcgctacca gtcgccttac aggctccatt
     1861 ctcgcttctc ttacgtgttt taacgattgc gccgataact aaacgaaata gacgctaaat
     1921 cgcttaaaac gcaaaataga ccctttttag agcattgatt ttttctacta gtcatttcga
     1981 taatataaaa ttgaccacaa cacagaaaac ttatattata agcttatgtc gtaagtccga
     2041 cgtactc
//
