LOCUS       AP031265                4052 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE19 plasmid pNUITMV19_9 DNA, complete
            sequence.
ACCESSION   AP031265
VERSION     AP031265.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639548
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE19
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 281x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE19"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV19_9"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            1778..2752
                     /locus_tag="NUITMVRE19_31300"
     CDS             1778..2752
                     /locus_tag="NUITMVRE19_31300"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="BFH03921.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVGN
                     LKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQLRRG
                     KATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYKYRNLL
                     KQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2749..2982
                     /locus_tag="NUITMVRE19_31310"
     CDS             2749..2982
                     /locus_tag="NUITMVRE19_31310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03922.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
                     /name="hypothetical protein"
     gene            2975..3400
                     /locus_tag="NUITMVRE19_31320"
     CDS             2975..3400
                     /locus_tag="NUITMVRE19_31320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03923.1"
                     /translation="MTDLSEKMTIKNLADELGVSKIKESEMVAVLEMRCNMDKIDLREE
                     TVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLNSTINVGLANSSLITDKFVYGKIA
                     ETEDESEKEFWLNILTYVMQLNQIGVIYRNKSKGKEL"
                     /name="hypothetical protein"
     gene            3404..3766
                     /locus_tag="NUITMVRE19_31330"
     CDS             3404..3766
                     /locus_tag="NUITMVRE19_31330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03924.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKKSQ
                     MSESKFTSMFNDVSLR"
                     /name="hypothetical protein"
     Typing          1857..2752
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.889"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1326
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.738"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 caaaaaatca atgactggca taagcatatt gatttcacaa aatcggatga aaatatctat
       61 ttagaacaaa caaatattcg tgaaaaatat gaagaattat tcggtgaagc cgtagaaaaa
      121 tataatgcaa agcagaaaag agccgataga aaaatcgagg actatttagc aaaggttcgc
      181 aaggataaaa aattggaacc acagcgagaa tttatcgtgc aaattggtac tcttgatgat
      241 tttcgtacta ctcgtgatga tggttcttct actggcatat cggaacaaca agcagaacaa
      301 aatcgagtga ttgccaacaa aattttagtt gaatatttca aagaatttaa agaacggaat
      361 cctaatttat ccgtctacaa tgctgttatt cataatgacg aagtaagtcc gcacctgcat
      421 ttgaatattg ttcctgttgc tgaagggtat aaacggggtg ttcaaaagca accaagtttt
      481 aacaaagcct tgcaacagca gggtttacga gcagataaag agaatggtcg agtgatttgg
      541 aataatttta ggaatcagga agttgagtca attgaacgtt tgatggcaga ttttggttgg
      601 gagcgtgagt tagtcggtac taacaaaatc aaagatgttc acgaatataa agaaatcatg
      661 agcgagattt cagaattgcg tcaaacggcc gtaaaagagc gtgagagcgt ttctgatgag
      721 ttgaggtata tttctaggca aagagaagaa aatgagcgag aaaaggctct ggtggcttct
      781 gagagagtga agattgaaga agagaagaaa aatatgctga atgtaaaaaa agagatggcg
      841 gtcattttcc aaaaagatat tgttccttta gaaagtttca agaggcttcc tgatggttcg
      901 tatcatttat ctgggaatga ttttgttgat ttgtatcaac gagctacgag agcaacaaat
      961 gccaagagaa atgctgataa catgagtgag caatatttac agatgcttga gcaaaataca
     1021 gaattaaatg agtatgtagc tacattggaa gaagagcgtc atttgtctgg tagaaaacaa
     1081 ttagcagatt tacaagtgga aaataaaaag ctgaaagaag aaaatcgctc tttacgccag
     1141 cgtttagagc gattaaaaaa cgcatttcaa aaatcgatca ctcgtttgtc tatccgtttt
     1201 ggtcttgcag aaaaggatat ggggctttct gaggaattag aagtgttaga gcaggagaaa
     1261 accttatttt atcgtcaaaa cgtctcacag cgtaaattag agcgttctga agatgagatc
     1321 gagtggtagt tacgatgtgc agtcaacggt accggaggcg gtttgtgccg aggataagga
     1381 gttgacggct aaaccactaa tccctgaagc tgacatttgc taaaaaatgg caagtccatt
     1441 ttttggccaa cctagcgtta gcgaagtttg ggggcgccct tttcaaaggg tgcggtttta
     1501 ttttttggat atttgcatag ctaaagagaa gtgctattat tgagttatca aataaataaa
     1561 ctgaaaaaag agcaaaaaaa taaagtcgag caacgctgga cacgttctcg acctttacgg
     1621 aaactaatcg cactagctcc gtaacttctt aatcattata cgaatatttt tagatttctt
     1681 caagactaga gattttttgg taatgaattt ttaagagtta cgtgaacttt tgaaaattct
     1741 ttccagaagt ctctttttct ttgaggagga acaaaatttg agtaaaaaag gcacgatcat
     1801 aaatgagtgg gaaaaattaa cagccccctc taacaggcgg ttaagtgtcc cttttatgga
     1861 caccccccta aaaattcagt ggtcaatcga ccgaattacc attgttggaa atcttaaaga
     1921 gaatatctat tatcacacta cacatgatgt gttgatttta gattttgaac agcttatgcg
     1981 cctaaatgaa ggtaacggtt atttaagatc tgttagtaat aatggttggc agttgttaga
     2041 ccaacatgaa gagaatattg cttatattga aattttaaaa tggcaagagg ggaaagggcg
     2101 tatagacttc aatccgaata agattagtca attcttagct agttccatga aaaattttat
     2161 tcatgatttg tttatcgaac ctcacttttc acgtgcagat attgcttgcg acattatcga
     2221 tgtacctgat gagtttatta cacaatatcg ggtagttgat ccagtttctt ttaagcctat
     2281 ttatggacgg aatggaaaat tagagacggc atattgggga tctcgctctt ctgaacgtca
     2341 aattcggatg tataataaaa aactggaaca agagaagaaa agaaaaattg tgccaaaaga
     2401 aatcgtgagt tggtggcggt tagaattaca attaagacga gggaaagcga ctgattggca
     2461 tgctatggtt tatgaaagct tagatagttt tgctagtcct cattacttgc ctgctgatac
     2521 aagtgtagct gacaaaatga tgattactgc tttaacaaca gaacatgatt attggggaca
     2581 aattaatcga aagacaaaat ataaatatcg aaatttgctg aagcaagaaa gtcaaaatga
     2641 tgaattgacg aatcatttac gagaaacctt tgcagaatcg gcagacgatt taaaaaaaga
     2701 attagacaca tggcttctag ggctagacgt aacagaggag gaagaaaaat gacagaaaaa
     2761 caaaagtatt ttggtaagaa aaattggttg gtaaatttaa caggtgatta cgttgtggtt
     2821 gtttttttgg atagcaaaaa aatgtccggt aagttacttg atgtaacgca atatgaattg
     2881 attttgaaaa ataataaaat tgactctccg attattatcc ctaagcacgc cgttaaatat
     2941 atttatgcgg tagctgaaag tagtggagaa gctaatgact gatttgtcag aaaagatgac
     3001 cataaaaaat cttgctgatg aattaggtgt atcgaaaatt aaagaatcgg aaatggtggc
     3061 agttttggaa atgaggtgta acatggataa aattgattta agagaagaga cagttgatag
     3121 aattgaattg atgtatgaac gattagctga ttatttgaat ttgccggatg aagataatta
     3181 tagtgaaatg cttgagagaa tgatggacgt tctcaattct acaatcaatg taggcctagc
     3241 aaattcttct ttgattactg ataaatttgt ttacggtaaa atagccgaaa cagaagatga
     3301 atctgaaaag gaattttggc tgaatatttt aacttatgtt atgcaattaa atcaaattgg
     3361 tgttatttat cgaaataaat caaaaggcaa ggagctgtaa atcttgggaa tgactttagc
     3421 agatattaaa gcacaagaag aaaaattgaa aaaagaacag gcaaaacttc gtcaacagaa
     3481 aaaacgattg attgagaagg aaaatgctga aatgggtaag gcacttcgga agcattttgg
     3541 atttgaatct tttgatgatt ttgagcggtg gttatcgtca atttctgacg atcagcaaca
     3601 cgaaaaatca gtggataatg agtggcttgc tgatgaaaaa tcagcgccaa gcgaacgaga
     3661 ttatcccaat gaatctgacg gcttactcgg agtgataccg agtaaaaaaa gtcagatgag
     3721 tgagagtaaa tttacgtcaa tgtttaatga cgtgagttta cgataagcga ttattttgcg
     3781 tgaagcaaaa taaagcgtaa aaggagttga gagcgtgaca cgctttcggc tccttttttc
     3841 gttgtcactt gtgacagcga ataccctcgg agagcatttg gaattgtgac agccatgtca
     3901 caattaggaa aatgttactc tttgacaaag agtaacagaa aggtttacac tctttttatt
     3961 agcgacagcc gaaggagggt tttaacttgg ggttcagtat ttcgttcaaa aagggaacaa
     4021 agaatacaag catttctcat aataatcgtg ag
//
