LOCUS       AP031262                7513 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE19 plasmid pNUITMV19_6 DNA, complete
            sequence.
ACCESSION   AP031262
VERSION     AP031262.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639548
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE19
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 281x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE19"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV19_6"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(466..1416)
                     /locus_tag="NUITMVRE19_31080"
     CDS             complement(466..1416)
                     /locus_tag="NUITMVRE19_31080"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFH03900.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFVYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVIGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPTTTINEKIKQKKQELELLQQI
                     KQLEKEKEALEKQTESALSEKEKKNLLSDLDHLFR"
                     /name="replication initiation protein"
     gene            complement(2293..2982)
                     /locus_tag="NUITMVRE19_31090"
     CDS             complement(2293..2982)
                     /locus_tag="NUITMVRE19_31090"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03901.1"
                     /translation="MAFYLPYLLIFVSISGSIWLSCKIYQTRHSLKGSKIRFKRFLLLG
                     CIFSLIIISSGLFGVLEGNKRISGSILLENVTQKYEVARDKKKKEQALTQKIEKFTACY
                     EDMSDIFVKQEKRLTDKNMETLTQLYRNLPEKQQKEYQEKYEQVKKDVQYVKDTQTEEA
                     CYDLFSDTIPFSTSEQERKERQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3222..4160)
                     /locus_tag="NUITMVRE19_31100"
     CDS             complement(3222..4160)
                     /locus_tag="NUITMVRE19_31100"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="BFH03902.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDHVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNPKTRTYDQFCDSLKIFGIHCFNRGKNFTYELIKKKKKVRS
                     NKLGKDYEKETILHELDRREQTYYRERERAYHRQQADELRRARESIRKIAGEVRREQRT
                     LTSEQPEIRPTQRNHEQHIERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4142..4531)
                     /gene="mobC_2"
                     /locus_tag="NUITMVRE19_31110"
     CDS             complement(4142..4531)
                     /gene="mobC_2"
                     /locus_tag="NUITMVRE19_31110"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002498952.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="BFH03903.1"
                     /translation="MGLMSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_2"
     gene            complement(4982..5416)
                     /locus_tag="NUITMVRE19_31120"
     CDS             complement(4982..5416)
                     /locus_tag="NUITMVRE19_31120"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03904.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="hypothetical protein"
     gene            5683..6177
                     /locus_tag="NUITMVRE19_31130"
     CDS             5683..6177
                     /locus_tag="NUITMVRE19_31130"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03905.1"
                     /translation="MKHAKFITVGLATITLLSATIPVGTTIVQADEIKNVSVENDQFKG
                     LSDNELRNLGFSEADIINYHDNLNNPVYIQEGMVLNSKEQHKERGKFTWAVKAIRKGYN
                     KLPKSVKRYIAAHTGLDTLLSFIENATGTIQDAVYNACRKVGMSKNVANIVTAAIMTLV
                     F"
                     /name="hypothetical protein"
     gene            complement(6200..6385)
                     /locus_tag="NUITMVRE19_31140"
     CDS             complement(6200..6385)
                     /locus_tag="NUITMVRE19_31140"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03906.1"
                     /translation="MKKIAEKKWFYWIIAFVILLFYILFYITKKNVFLIAFLVSWISFT
                     LYGFYLILHELNKKSD"
                     /name="hypothetical protein"
     gene            complement(6369..6818)
                     /locus_tag="NUITMVRE19_31150"
     CDS             complement(6369..6818)
                     /locus_tag="NUITMVRE19_31150"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03907.1"
                     /translation="MTVGSTLKYHRKKANLSQIELAHKLHVSRQSISSWENDHSYPSLD
                     NLIALSRIYQTSIEDLLKDNESLRNRLSVHYDQLLECEQKIYQIQTQVDNLNQDLNYDK
                     EDEFLFCITLIFISITLAPLGLVIPIIYFINKKKKRKKRLFEKDS"
                     /name="hypothetical protein"
     gene            complement(7251..7421)
                     /locus_tag="NUITMVRE19_31160"
     CDS             complement(7251..7421)
                     /locus_tag="NUITMVRE19_31160"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH03908.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     Typing          complement(467..1415)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.183"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3226..4160)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.705"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctgaagttt cttccgtata aactaattgt aaccgttttt gtagtttgtc gatttgttga
       61 tttgcctgca aggtgagttg ttgttgctgg tcgagtaact tttgcatttg ttttatttga
      121 gtatcttttt cttggagttg ttcacgtaaa aatttatttt cttcacgcaa ctgttctagc
      181 aactcatttg caaccacgtt tttggttggt atataagcat ttttatcgct gttttgttca
      241 tgttcatgca acttatctag caacctttgg ttgcctaaat tgcgagcaag ttgcaatact
      301 ccagaacgca atacaacctt ataaccacgc tcatttttag ctatcatgga agagtctaat
      361 ttttggatat gttttgatag tgcttgccta cttatcttaa aaagttctga caattccttt
      421 attgtaaagg tttcttgtgg ttgcatattg gttgcctcct ggttgctacc ggaataaatg
      481 atctaaatca gataataaat tttttttctc tttttccgaa agtgcagact ctgtttgttt
      541 ttcaagagct tctttctctt tttctagttg tttgatttgt tggaggagtt caagttcttg
      601 tttcttctgt ttgatttttt cgttaattgt tgtagtaggc tcttcgtatt ctttttgttt
      661 atcagctaaa gccaatgctt tattttgttc ttctttggtt ggtgcatgtt tgttaaaatc
      721 gtctgctttt ttgatttctg gtttaaacgt aaattgatag ccaatgacag gttttcctcg
      781 cccttttccg tatttctttt taattgataa tcctttgaag attgcggtta attcctgacg
      841 tataggcttt ataacacgat tatcaacgtt tgcttggttg ttccagtaac tttttggaat
      901 atctaatagt tcatgaaaat cctctttaga aaaataaaca aaacctttag ttcgatattg
      961 ttttagtaat cgaaacatag tttttgcata gctactttgt aattcgttaa attgttggag
     1021 tgaatagcga acccactcat ctagattgtt tagtaaaggt aaagcttttt cgtaaacttg
     1081 tatatctaca tatggatctt tagcagttcc acgtatttta aactcactaa acataacgaa
     1141 tctatctata tctagcccat tagcactcct tttgccaaaa cgtaaatcca ttaatttatc
     1201 ataagtcatt tctaaatcat tgatgaaacg attattagca gttgctttgt aattactcaa
     1261 atcttttagc tggtcaaaag taagacgaac ggttttatcg cctttttcgc gcattctaga
     1321 cacaatagag aaaaatagat tcatttcaac tggtgtaaat ttccgcaacg gaatggtatt
     1381 taattcatga tggtatttca cgatttcatt tgccaaatct attcctcctt actctcccaa
     1441 aggatacaga cgttataaac aacgttttgt ctgtgataat acatattata acatacgtga
     1501 gtataaaaaa agagtttaaa agcagacaaa atgtagttta aaagcagaca aaatgtagtt
     1561 taaaagcaga caaaatgtag tttaaaagca gacaaaatgt agtttaaaaa acgatgaatc
     1621 ccttgagaga gtaagaacat aaccgacagt aaagaaggtt aaaaagaggt ttaaaaagag
     1681 atataaaaag aggggggcaa aatttcccgc ctaaaaaatc actatttttt tcctaaaaca
     1741 ggttcgcgca tttttttaaa ctgccaatct tgctccctac ggtcgctaaa aaggtgtgat
     1801 cgctatgctc tcacattgta ctgcgcagtc gcctaaaggc tcctttgcca aaaccgttct
     1861 tcaaagagaa ccttgtgggg ctctgatagc tcgtctaaac cgttttgaga cagttctagg
     1921 ggaaaaccct tatctagttc ctaaagcacc ttacaggcgg tcagggcaag ccctaacaac
     1981 cctaaaataa gggatactta aaaacaccac acagatttaa ttttttaaga tataataact
     2041 tgtcgcctat ccgacagtct tgcgactgga aggaggtgta tcttatgatt acccacttta
     2101 tttgcgccct tcttgttgag cttctaaact ctttatttag ttattggctg aataaccgaa
     2161 acaagaagta aaatcaacta aggcgatatc agctcacaaa aaaagtccta gagaatccga
     2221 cttctctagg actttcgtgt atcttatgat tacctatttg catatttagt ttaacatgag
     2281 tttcaatcaa attcaatttt gctgttgctg atctaaccat tccttgacac tttttagata
     2341 gtttaaggcg gtttcttttt ttgtcggatt ttgcatgtta gttgcttgtt gaagcaagat
     2401 ttcatagcgt tcatatgtga ctgattgttg tcgttctttt cgttcttgtt cgctagttga
     2461 aaaagggatt gtatcactga ataagtcata acacgcttct tccgtttgcg tatctttcac
     2521 atattgcacg tcttttttta cttgttcata tttttcttgg tactcttttt gctgtttttc
     2581 tggcaaattc cgataaagtt gagtgagtgt ttccatattt ttatccgtca aacgcttttc
     2641 ttgcttcaca aaaatatcac tcatgtcctc ataacaagcg gtaaattttt ctattttttg
     2701 cgttaatgct tgctcttttt tctttttatc acgtgctact tcatactttt gagtgacatt
     2761 ttctaataaa atacttcctg aaatgcgctt attgccctct aaaacgccga ataagccgct
     2821 tgaaatgatt attaaggaaa atatacatcc gagcaataaa aaccgcttaa atcgaatttt
     2881 agagcctttc aatgaatgtc ttgtttgata aattttacag ctgagccaaa tacttcctga
     2941 aatactaaca aaaatgagta gatagggtaa ataaaatgcc attttagcct ccccatacta
     3001 accaaaatgc gaacagtgtc aggactaagc aacataaggg ggcaagaaaa tccattgatc
     3061 gttgaatgag cgcctttttt tgttctttat ttcgctgatc taatagctct tgatggagtt
     3121 ttttaacttg taaatcaatc gtagtttgta tcctttcttg tgtgtaggga gcaagaaaag
     3181 cacgttgttt ctttatttcg ttttctacgt ctcgtaacgt tttagagcga taattcttgt
     3241 tggtggtctg ttcctctttc aatgtgttgt tcatgatttc tttgagtcgg tctaatttct
     3301 ggttgttcag acgtaagagt tcgttgttct cttcgtactt ctccagcaat tttccgaatg
     3361 ctttctcgag ctcttcgaag ctcgtcggct tgctgtctat gataggctcg ttctcgttct
     3421 cgataatagg tttgttctcg tctgtccagc tcatgtaaaa ttgtctcctt ttcatagtcc
     3481 tttcctaatt tgttggatcg tacttttttc ttttttttga ttaattcgta agtaaaattt
     3541 ttcccacgat tgaagcaatg aatcccaaat attttgaggg aatcacaaaa ttggtcatag
     3601 gttctggttt ttggattttt catggtttga tcaatggcaa agcgaatctc gtctttccaa
     3661 acaggttcat ttttttcttt tagtttgatt tcagacatag ttcgtttttc ttcttttttt
     3721 cctcgttcag tgatacttaa accatgagtc aaacaaattt cgtcgttgat atcgtaacac
     3781 ttatctagga atgcttgacc atgtgcttga aacttttttc ctgtttctaa attgaccgaa
     3841 ttaatgacaa tgtgattgtg gatatggtct ttgtccgtgt gcgtataaat cgcgatttgg
     3901 tgttctggtg cgattttttc agcgtattct tttcctagct ggttggcttt ttcggcggta
     3961 atttctcctg gtcgaaagga ctgaatcaat aagtgtgctt gtacatgatc gtcttttccg
     4021 taaagcatac gtatttgttt catttcactt ttggcataat caatgtcaca gtttattcct
     4081 gttttgacgg ttgctcgtgg ttctgcgtaa ttgattgctc ggctacagga agtggttcga
     4141 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     4201 ataagcgttg ggcgtcgcca ggagcaacgt cgtgttggtg tgtattgcac cacttggcaa
     4261 tttgattgat attccctcca gctttggcta aatcgcgcgc gattgcctgg gcaatgtctg
     4321 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     4381 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     4441 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaagga tcgctagcca
     4501 aattttgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     4561 tcgcaatatc attgtaccta aaaaggtaca atgagtaaag ctcactaaaa gggtttggca
     4621 agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc ttgtcaaagg
     4681 ggcgcaagct cccccttgaa tccccctcaa aactcacgct ccaccataca aaaaaggcta
     4741 gcaaatgcta gccacttttt gtaaaattta gtatgattta tttttagcta tttagagttt
     4801 ttatttccgt aaatacttct aaaaattgct aagataaatg ctataggaat tagataaaga
     4861 atactttttt gtaagcttat attgaaaatt agcctaaata tgaagaacat agtgattaaa
     4921 actattattg aaataattac agctaaaatc tttttattca tgagatagtt tcactttctg
     4981 attattttat tacataacga gcgtaatcat tgtattgata tgatgttgta ttgctaaagt
     5041 gagcattata tgttaattgt acaccttttc cttgatttgc agctcttaca aagacattca
     5101 ttgcatcaac agcactacta aaagcatttg taataaccca tgttccaact ccaccaatag
     5161 gaataaaaga tgcaatttga gcaggagtct ttagcttatt ataatttttt ttcaaatctc
     5221 tacacatttt attagtaaca taatagttca ttttatagca agtaaatcca cgatgattat
     5281 gattatatcg tgaacttgca gcttccactt ttatacctct agcactaaaa ggaaacagat
     5341 ttaacggagt acttgctcca acaatcatac caaacaaaaa tagacaagta ataattttaa
     5401 taattttatt tttcattgtt acttcctcct tagatataaa atcctaagaa agcgctttct
     5461 gtgtatattt agaataatca gaaaattaaa tattgtcaag agggtttatt ttataaaatg
     5521 tgttatttta aaaattagta gacataatgg ttattaaaaa aatataaatt aataatatat
     5581 atgtaaagct atgtaaagtt aaaatttctt ttatttattt aataagctag ataagataat
     5641 tttgtaattt ttatacaaaa tatttctagg agtgaaacaa ttatgaaaca tgctaaattt
     5701 attactgttg gattagcaac tattacttta ttatctgcta caattcctgt gggaacaaca
     5761 attgtacaag cagatgaaat taaaaatgtc tctgtagaaa atgatcaatt taaaggtttg
     5821 agcgataatg aattgagaaa tttaggtttt tcagaagcag atattataaa ctatcatgac
     5881 aatttaaata atcctgttta tattcaagaa ggaatggttc taaatagtaa agaacaacat
     5941 aaagaaagag gaaaatttac ttgggcagta aaggctatta gaaagggata taataaatta
     6001 cctaaatcag ttaaaagata tattgcggca catacagggc tagatactct tttaagtttt
     6061 atcgaaaatg cgactggtac aattcaagat gctgtatata atgcatgtag aaaagttggc
     6121 atgagtaaaa atgtagcaaa cattgtaact gcagctatta tgactttagt attttaataa
     6181 aaaatccgat tagttttaac taatcggatt ttttattaag ttcatgtaga attagataaa
     6241 aaccataaag ggtaaaacta atccatgata caagaaaagc tattaagaaa acattttttt
     6301 tagtgatata gaaaagtata taaaataaaa gtataacaaa agcaataatc caatagaacc
     6361 attttttttc agctatcttt ttcaaataat ctcttctttc tttttttctt tttattaata
     6421 aagtaaataa taggaataac aagtcctaaa ggagctagag ttatacttat aaatattaaa
     6481 gtaatacaaa ataaaaattc atcttcttta tcatagttta aatcttggtt taaattatct
     6541 acttgagttt gtatttgata tattttttgc tcacattcta atagttgatc ataatggaca
     6601 gataacctat ttcttaatga ttcattatct tttaataagt cttctataga cgtttgataa
     6661 attctagata aagctattaa attatctaaa gaaggataag aatgatcgtt ttcccaacta
     6721 gatatagatt gacgacttac atgtaattta tgtgcaagtt caatttgaga taaatttgct
     6781 ttttttctat gatatttcaa agtacttcct acagtcatta gttaatcctt ttatcaaata
     6841 atagtaccct aatattgtac aatgatgata tctgattttg aattatattt cttcaatgat
     6901 tattttaagt tcataatcgt ttaaataaaa ttataagatc agttatttaa acagctagta
     6961 gacataatgt acagtatgcc aagtagcccc tctaccaatg gatagagacg ctcactttgt
     7021 gatttttaat tggtctagct aaaagcaact ttctaaagct tgatttttcg cggttatcta
     7081 tatctaattc tttgtgcatt tttatctgtc attttttaaa gacttgttat ttttgagagg
     7141 atttgatatg ctttttttat ctttcttata gttcttcatt ttgtaatatt tttatgttga
     7201 tgaataggat aatctcattt tgggctgctc gttagagcag tcttttttta ttatttatcc
     7261 gtttctttta caaataactc ttcgttagga ctgttgtaat aatttggatt caatttcaat
     7321 tcaccatgtt tatttccttt attgatcact gacaataaca tcggactgaa gcctagaatt
     7381 cctagtaaaa tagaaccaaa tatgagcttt tttcttgtca tgaatgtttc ctccagttat
     7441 ttccatagtt tccaccattt ttgcttcttc tttgtttgtt tttctatcga ttctgtttct
     7501 tcagaaagag tag
//
