LOCUS       AP031254                1964 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE12 plasmid pNUITMV12_10 DNA, complete
            sequence.
ACCESSION   AP031254
VERSION     AP031254.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639541
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 160x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE12"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV12_10"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            47..1063
                     /locus_tag="NUITMVRE12_31860"
     CDS             47..1063
                     /locus_tag="NUITMVRE12_31860"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="BFH00794.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGTT
                     TPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQLF
                     DQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIACDI
                     VDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERKKVIV
                     PKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGLIAEPS
                     NWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          48..1057
                     /name="rep_cluster_935"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.292"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctggaaattc tttccaaaag tctctttttt cgtttggagg acgaaaatgg caaatgaaaa
       61 tcagttgagt gaaattcaat caaaaattga aggtcgaggg gcagaaaaaa gaacaacgat
      121 aaatcagctc gaaaaactta accgtatttc taacaggcgg ttaagtgtgc aaggtacgac
      181 caccccccta aaattaaatt ggtcgattga tcgaattaca attgtcggaa aactgaatga
      241 taccatagct tattggacga aaaatggagt tgtttttgtc gatttcgagc aattaatgcg
      301 tttgaacgaa aatcagggat atttgaaatc agttgggata aacggctggc aattattcga
      361 tcaatatgga gaagatattg cgtatatcga aatacttaaa tttcatgaag gaaaaggtcg
      421 tattgatttt aatccgaata agataggcca gtttttagct agttccatga aaggattcat
      481 tcatgaccta tttttagatc cgcatttttc ccgggcggat attgcctgcg atattgtaga
      541 tgtgccggat gactttatta ctcagtatcg tattgtagac ccgatttctt ttaagccaat
      601 ctacggccgt tctggcaagc tagaaacagc gtactggggc agtcgtgcca gtgagcgcca
      661 agtgcgactg tacaacaaaa agctagagca ggaacgaaaa aaagtaatcg tgccaaaaga
      721 aatcgatacg tggtggcggt tagaaatgca gctaagacgt ggcaaagcaa cggattggca
      781 tgcgatggtt caggaaagct tagatagttt tgcaagcccg cattttttgc cgattgatat
      841 aaaacctatt gataaaatag ttattgatgg tttgatagct gagccttcta attggtcgat
      901 aattgctaga catacaaaat ataaatatcg gaatttgctg aagcaagaaa gtcaaaatga
      961 tgagttaacg aatcatttac gagagacttt taaggaatct gcagacgaat taaaaaaaga
     1021 attggatacg tggcttttgg ggttggatgt gacagagaag tgataccgaa taagaaaagt
     1081 cagatgagtg agagtaaatt tacgtcaatg tttagtgacg tggatttacg ataagcgatt
     1141 attttgcgtg aagcaaaata aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc
     1201 cttttttcgt tgtcacttgt gacagcgaat accctcggag agcatttgga attgtgacag
     1261 ccatgtcaca attaggaaaa tgttactctt tgtcaaagag taacagaaag gtttacactc
     1321 tttttattag cgacagcgtg aaggaggggt tcaccttagg gtttagtatt tcgttcaaaa
     1381 agggaacaaa gaatacaagc atttctcata ataatcgtga gttaactgat agccaaaaaa
     1441 tcaatggttt gtctggtaga aaataattag cagatttaca agtgggaaat aaaaagctga
     1501 aagaagaaaa ttgcatttca aactacattt tctacaatag ctctaaaatg gcatattttg
     1561 cgttctgagc ggttttagcg tttatttcgt ttagttatcg gcacaatcgt taaaacacgt
     1621 tagaggagcg aaaatggagc ctgtaaggcg actggtagcg gaacgatcgt gttagattgt
     1681 gaaaaccgat agctgaatga aataaacagc gggtgcgccc tttccatttc ggcaggagga
     1741 ggctcaaggg gttcggggaa tgaaattccc tgaggtcttt ttattttttt aggaggagtg
     1801 ctataatcaa agaaaacgag aaaaaagagc aaaataaaag ccgagcagtg cgctaacact
     1861 ctcgactatt tacggaaact acttcaccta gctccgtaac tcaggttcat tatacgaata
     1921 tttttaggtt tcttcaagct agagattttt ggatatgaat tttt
//
