LOCUS       AP031253                2048 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE12 plasmid pNUITMV12_9 DNA, complete
            sequence.
ACCESSION   AP031253
VERSION     AP031253.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639541
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 160x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE12"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV12_9"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(254..856)
                     /locus_tag="NUITMVRE12_31830"
     CDS             complement(254..856)
                     /locus_tag="NUITMVRE12_31830"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002919940.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="BFH00791.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(923..1078)
                     /locus_tag="NUITMVRE12_31840"
     CDS             complement(923..1078)
                     /locus_tag="NUITMVRE12_31840"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00792.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="hypothetical protein"
     gene            1696..1917
                     /locus_tag="NUITMVRE12_31850"
     CDS             1696..1917
                     /locus_tag="NUITMVRE12_31850"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00793.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     Typing          21..196
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(327..856)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.812"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 attaaactct gaagaacaaa aagagcataa gggaaaactg atagcacctt cctacaggaa
       61 ggcggctaag tcggctgtgc caactggttt tcttacaaaa caaccttata ttttttgata
      121 tcacaaaaaa atccattttg gacctatttt cggtcataat atagtaccta cttttggtca
      181 tagtttcgtc cgtagttttg gtcatagtga ctaagacttt cacgcacaaa aaaagagctg
      241 aaattaacag ctcttatcct ttatttattt ttgaatttac acttccacca tgacgattcg
      301 attttatata ttgcccgata atataagctg aattatcaca aagaagcgga aaccaatcat
      361 caaatctttc tgacattgca tagtcaatta aatctttgat ttcagtgata ttcttagaat
      421 caacaaacga catcatctct ctaatcaact catatcgttc tgcactcgtt actgacaaat
      481 aactagcaat ttctgcacca ccatgagcga taatatcact tttagcatat tgaaattttt
      541 ctggattatc catatgagca aaatatcgca ccataccttt tgcattagca catttttgcg
      601 ggtttggaga ccgaatcttt tgagttatct cacgtatttg atcatacgtc tttttaccgc
      661 taaacattat caaaacatgc caatgaggct ttttcaattc accgtcagga ttcacatctt
      721 tatcatgaag tggactctca acccaaggaa catgtaactc atctaaaaac tccctccaat
      781 tttcaggagc cgattcagga tagactacaa acgtccaatt ccttgtgcgt tcatctttag
      841 tttttctact cgtcatgcta taatcacctt aattgaacaa cagtctaaca gcaaaagcag
      901 tgcgccaaca ctaatgcttt tgctattttt tttgctcatt ttcctttccg ttagcattaa
      961 cccaaattgt aataagacca gacttactaa gacccatttg tttcgcaatt ttatcaagat
     1021 tatctgccac ttccttagta agcgttacaa cgactctctt tttttcatta ctgatcataa
     1081 ttcgtatcct ccaacgactt tttcttaaaa accatagtaa caaaaaagta actaaggtgc
     1141 aacagtttag atactaatta gcgaataata ggccttaaac ccttggcatt actgattttg
     1201 tcaaaaaaaa acgctgaaac ccttgggaga gtaaggctaa acagctatcg accaatgacc
     1261 aatgaccaat aaggggggtg tcgtagtccc ccccctcctc cgcccaattc ataaacacca
     1321 aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg acctccttct accgaaatat
     1381 atgcaggcgc cacgcttact atttcgttca gctatcggct tttgcaatct aacacgaccg
     1441 tttcgctacc agtcgcctta caggctccat tctcgcttct cttacgtgtt ttaacgattg
     1501 cgccgataac taaacgaaat agacgctaaa tcgcttaaaa cgcaaaatag acccttttta
     1561 gagcattgat tttttctact agtcatttcg ataatataaa attgaccaca acacagaaaa
     1621 cttatattat aagcttatgt cgtaagtccg acgtactcac gtaatataga cggacgttaa
     1681 atggaaggag cataaatgag agtgataaaa tcagaaaaat tcactttggc cgaaattatg
     1741 acgaccagcg aacttgcaca agaacttgat tacaaccaag cttacgtttt gcgcttagcg
     1801 aaagaacatc tgactgaagg aaaagaatat cgctctgccg gtagaagaaa ctatcttttc
     1861 agcccagaag cccttaaaaa actagaaaaa gtcataacca ccaaagaagg tgagtaaatg
     1921 cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat tgctagaatc atattccaat
     1981 gattttatcg aattgaccgg cggaaaagtc gaagaaacca ccgacctctt tgacctactt
     2041 ctcacaaa
//
