LOCUS       AP031252                5248 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE12 plasmid pNUITMV12_8 DNA, complete
            sequence.
ACCESSION   AP031252
VERSION     AP031252.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639541
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 160x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE12"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV12_8"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            604..1059
                     /locus_tag="NUITMVRE12_31760"
     CDS             604..1059
                     /locus_tag="NUITMVRE12_31760"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00784.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGYI
                     KVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQVQ
                     KFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
                     /name="hypothetical protein"
     gene            1087..1584
                     /locus_tag="NUITMVRE12_31770"
     CDS             1087..1584
                     /locus_tag="NUITMVRE12_31770"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00785.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRVV
                     FALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQAL
                     KIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYFELN
                     SQ"
                     /name="hypothetical protein"
     gene            2551..3300
                     /locus_tag="NUITMVRE12_31780"
     CDS             2551..3300
                     /locus_tag="NUITMVRE12_31780"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00786.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVLD
                     YCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVERP
                     DGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGKYAL
                     IMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELSDKMS
                     VSFFVTTLKRGRKVVGYEITINDTSKE"
                     /name="hypothetical protein"
     gene            3314..3463
                     /locus_tag="NUITMVRE12_31790"
     CDS             3314..3463
                     /locus_tag="NUITMVRE12_31790"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00787.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGEQ
                     KNDG"
                     /name="hypothetical protein"
     gene            3453..3614
                     /locus_tag="NUITMVRE12_31800"
     CDS             3453..3614
                     /locus_tag="NUITMVRE12_31800"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00788.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLTE
                     IIELDSKE"
                     /name="hypothetical protein"
     gene            3623..3988
                     /locus_tag="NUITMVRE12_31810"
     CDS             3623..3988
                     /locus_tag="NUITMVRE12_31810"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00789.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFMR
                     NASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLDYK
                     TFYPELLEFFQKLNSLE"
                     /name="hypothetical protein"
     gene            4544..4900
                     /locus_tag="NUITMVRE12_31820"
     CDS             4544..4900
                     /locus_tag="NUITMVRE12_31820"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00790.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLCK
                     SVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQQL
                     SQIEKGVIDLWEQL"
                     /name="hypothetical protein"
ORIGIN
        1 ccggtaaaaa atttcactct gataccgagg tcggctacca cctacaagac ctaaacgata
       61 aggttttatt gagttttggc attgaacagc cgaaagaaaa attcaaacaa gttgattttg
      121 aaatgcacgc cgagggcaaa atgagtaatc gagaaaaaat gaagcatatg atcctgggcg
      181 cacaagatat gagtaccgat tttgattctt tcaaaaaaaa tcttcaagaa gcctatgctg
      241 tcgaggttta tgagtacaat aaaggcaaaa ggattggctt caaatgggtc gatgatgagg
      301 gcaaagattt cacaatcggc ggacgaaaat taggttctga ttttgagcgc cctgcgatcg
      361 aacgaacact tgaattttct caacaaaaac aagaaaaaga agtcgaaaaa ccgcttgaaa
      421 ccaagcctaa agtcaccaat gatgactttt ctatcagcgg tttgaaaaat cttgctaatg
      481 atattcgtca gcaagaagaa cgcaagaaaa aagaggagct gcaacgacaa aaagaaatcg
      541 agcgtcaaca acgttctcgc ccgttctatc atgatcgtgg tggcatggaa aggtagtgac
      601 gatatgccgt tagatgattt tgcaagtaag caggagcttc aaaaagtgaa gcaggaacta
      661 gcggaaatca agcaacaaaa tcaagaagtc attgaaacgc tgaaaagcct tgataacagc
      721 gtaaaacaag gctatatcaa ggtgcgaggt ggcgaagata ttcccaaaat gagcgagctg
      781 aaagctgaaa tgaaccgcta cgccacctac gttcaaaaaa gaaccgctga aatcgaccaa
      841 aagcttgata gctacggtaa tatcagcaaa tctgtatctg acgaattgag ccggcaagtg
      901 cgcccacaag tccaaaaatt tttagccaac gatgatcgag cgactgccga agagctgaaa
      961 caaaatcgaa tagcgaattt aatgtccgta ttagcctctg tggggctttt tatcggtggc
     1021 actgctattc ttattgtttc agtcctaaag ctcctgtaat cgaacgagag ggggcttaaa
     1081 acgcaaatga aactgttctt tgaaaaaatg gggtggcata ttcttcctgt cgtgattatt
     1141 ttggtcggtt cgtatagttt attctgcatt tttcgctcga tcttagaaaa atttgaggaa
     1201 ccaaagctga aacgtgtcgt ttttgccctc tgttttgcgt ttctagcgct ttttgtgtcg
     1261 gtcggcattt cttcataccg tccggacgtt gcgctgtctg tacgccctaa aatcagcaaa
     1321 atagagcaac tagaagcgaa agccgaagca aaacagaaaa aaattgataa aatttcggac
     1381 caactggagc aggcattgaa aattgccagt caagaaaagc tgaaaacctt taacccaaaa
     1441 gactatccaa gcgaagttat tacggcggat tgggtacaaa aaaatcgtga cgtgatcgct
     1501 actttgcccg ataaagagct gaaagaccgc tacacaaagc gatttgacga tgtagaatcc
     1561 tattttgagc tgaatagtca gtgaaattga gatttgccaa cgtccatgct ataataacca
     1621 tgcgaaaacg ttagaatttt ttcgagtgcg gaatgcacaa aagccagaca ccatgcgacg
     1681 gtgtctggct tttttttcgc cctgcgacta ggcgtgtgag ttggaaggca ctaagcctta
     1741 gttgtcgtct ttctcgtcca accaatgaga aactagctca atgactagcc cgacaaatag
     1801 cggtgcgaca accaacgtta gaaatttttc gtacatacgg aatacacctc ccaacctgtg
     1861 taagcgttag ttggctttag tgccaagatt aatatagcat tcactccctg caaaaaaaag
     1921 atggtctacc agatggggct tttgggtcgt tagggcttgc cctgaccgcc tgtaaaggcg
     1981 ctgtataaca agactaacga tttaatctaa atcagtctaa aaacttgcta caacaagctt
     2041 ttacgcctat cccaaccgct ccttgaacaa cggttttgac cttaacgaag gagcgcaggc
     2101 gactgctgta agcaaaatgt gagagcgtag cgatcactcc tttatgcctt gtggctgtaa
     2161 gattgaacga ggtaaagggg ataaattgtc gatgatgtga gaagcagttt atttcatgcg
     2221 cgaacctgtt ggaaaaaaat agacaactgg tttgggcggg attttttttt ttttgaattt
     2281 aaattttttt ctcacccgcg cgcttaaaga gaatacttta tagattactt ttaaatactt
     2341 taaaatactt taggggaaaa ggaatggctt tataccagcg tttgtagcgc ctataaatac
     2401 tattttccac gttgaaaata ctattttcca cgtcaaaaat actattttcc acgtcaaaaa
     2461 tactattttc cacgttgaaa attcaaatat tgcaaaaaaa ctgaataaat ggtattattt
     2521 taaaaaaagt acaaaaggag gattttcagt ttgggacaac caaataaaaa agctgatatt
     2581 gcattaaaag acttactaag ccgtcaagat tatttagtag tgcaaggaaa cgacttagct
     2641 aaggcatttg gagggcttaa atcgtttgag caacgagttt tagactactg ttttagcttt
     2701 gtaactaaag attcaaaact agatgacgaa tacgaggtaa ccgcattaga aattatcaaa
     2761 cattttgggt tgaacacctc tggagattct taccgtcgaa tagctgaagc tttcaaaaag
     2821 cttaatgaaa atactgcgct ttatcttcgt gtcgagcgcc ccgatggtac tcaaggtctc
     2881 agaatgacgc aattattttc ttggattgat ttttatgctg acgggttagt aagattcaaa
     2941 ttttctgctg attcagctcc atacgttctt gatctcaaag aaaagtttta tagctttcgt
     3001 ttaggtgaat tatctcggat caatgggaaa tatgctctca taatgttaaa actctgggaa
     3061 tcctaccgta gaggaaaaga aaaacacaca acgattacag gtgatttgga agaatgggaa
     3121 tcttggtttc ttggtaaaga caaacgcctt cctgcaggtc aattttttca aaaagtaatt
     3181 actcgtgcta ttgaagaatt atctgataag atgtcagttt catttttcgt cacaacctta
     3241 aagcgtgggc gaaaagtcgt tggatatgaa atcacgatca atgacaccag caaagaatag
     3301 aggagtgatt tagttgaaaa aaagactgcg taaaaagatg gcaaagaaaa attcttcgct
     3361 agatcaagct atgaaagaac taatgaacta tgcaaaaggc gaacgtgacg gctttttgat
     3421 tcatagttat gacgaaaaag gagaacaaaa aaatgacggc taagacagtt acgattgacg
     3481 ttgaacagct tgaaaagctg ttagaaaggc atagtgacgc tattattgaa tacaccggtg
     3541 gactttcaga agaaacgatc aaccaatttg atcttcttct aacggaaatt atcgaactag
     3601 attctaagga gtgagtttac gcatggcaaa aatggatatc gaagatttgg aaaaaaaatt
     3661 agctgaagct cggcatgatc tttcagtcaa aatcggtaca aatatggttg aaaaacaagg
     3721 gttcaagagc tattccgcct acaatacttt catgcggaat gcgtcaaagg cattgaagtc
     3781 gtccgccgga tctgcacctg cgccagcagc tgcaaagccg gtggccactt ctgccgaaaa
     3841 attgcctagc gagctgaaag ccgaagcggt gcaatttcaa aagcaactcg aaaaatggat
     3901 ggaagacaac tggggaaaca agttggatta caaaacattt tatcctgagt tgctagagtt
     3961 cttccaaaaa ttgaacagct tggaatagcg gaacgagcta gtccatgcga tcaatttttg
     4021 aagaacgagc aagccgattt ttggcttgct tttttgtttg cctgcgaaca gcgggcaaac
     4081 aaaaaaataa ggtcaacttg cgtgcaagtt tgaccgcatg agccagtaga aaaatagtcg
     4141 aacagactac tttttctgag gcgaaatcac gtaataaaaa tagccgaaca ggctactttt
     4201 tgttgcgtga ttgggggagt tcgtaggggg caacgccacc tcgacaagcg atgtatttgg
     4261 gtaccaaatg cggtgcttgc gtattatatg atttattaac cttgttatgc gaccgaatag
     4321 ggagtatgat gaggttaata aatcattcgg ttatagagcg gaaaaaggga agtgaccgag
     4381 tagggaactt tcattttgta gtggcataac cgagggagat ttcattgcgg aaaaaaggcg
     4441 actgaatagg gagcgttttt gtagctttga atatcgacca cgaagaatga cgagaagaaa
     4501 aaagcgaccg aatagggagc atttttattt gaggagtgat gagatgagcg agcagaaatt
     4561 gaatgccgaa aatcgaaaag atagtcggca aattaaattt cgagtgagcg aagacgagta
     4621 ccagtatttg accgccattg cggatcaaac tggtatgagt gtgccacagc tctgcaagag
     4681 cgtggcactt ggcatgaagt atcgagcgcc gaacgttgat cgggaggggg cgatacaaat
     4741 tgccaaagag ttgcgcaaat atggcggaaa tctcaatcag atggctcgct ttattaacat
     4801 gaataagggg caaatgcctt caagttcaga tttagccttg atgaaccagc aactttcgca
     4861 aatcgaaaag ggggtgattg acttatggga gcaactttag ctttaaaagg cggtaaacgt
     4921 ggggcttctg cgctgattgg ctatgtaaat cggcatggaa agcttgatcg tgaccgcatt
     4981 tatacgcaca aactgtcagc tgagaataaa ttggctcaaa ctcagctaga agcgactaga
     5041 cggattttcg ccaaaacagg cggtattcaa gcctttcatt gggttcaatc gtttgataat
     5101 tcgtttgaca aaaacaatcc ggcggacgtg caaaaagcgc atgagatcgg tgctaaactc
     5161 gttgatgaaa tcgcaaaaag ctatcccaat catgaaatct acatgggcac tcataccgat
     5221 tcagaatccg gctatattca taatcaca
//
