LOCUS       AP031251                5929 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE12 plasmid pNUITMV12_7 DNA, complete
            sequence.
ACCESSION   AP031251
VERSION     AP031251.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639541
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 160x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE12"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV12_7"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            1587..2555
                     /locus_tag="NUITMVRE12_31720"
     CDS             1587..2555
                     /locus_tag="NUITMVRE12_31720"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFH00780.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            2572..3096
                     /locus_tag="NUITMVRE12_31730"
     CDS             2572..3096
                     /locus_tag="NUITMVRE12_31730"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFH00781.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            3472..3753
                     /locus_tag="NUITMVRE12_31740"
     CDS             3472..3753
                     /locus_tag="NUITMVRE12_31740"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390111.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00782.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            3755..4171
                     /locus_tag="NUITMVRE12_31750"
     CDS             3755..4171
                     /locus_tag="NUITMVRE12_31750"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390085.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00783.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     Typing          1588..2551
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaataaaccc attgctttag ttatgggtct agtgttagtt cttttcttaa ctagttgttc
       61 aaaaaaacaa gaaatacaaa agactgatct gattcctttt ctaggaactt ggcaaactaa
      121 agatcattca gaacgtctag taatcactaa tttaaatact tcaaaaaata tagaacaata
      181 taccatctta gtaaaagaga acaatcataa aattcaaatc attgataaaa atttaaatat
      241 aaatgaacaa gttttcattt acaaagcaac aaataattta ctaacactgc gctctaataa
      301 aaaacaatta gattacactt ttagtttgga ttctccaaaa aagctcgtat ttattttgac
      361 aacgaatgat gggacagaag ggacagctaa accaattgaa ttttttaaag ctgattgatg
      421 aatagttgtt tgatattaca aaacaataat ttcaccgaag gtgaaacaag cgaaacttta
      481 caaaaagtgg ctagcatttg ctagcctttt ttgtattgtg gagcgtgggg gggaggggga
      541 ttcaaggggg agcttgcgcc cctttgacaa gcggttattt gcaccaaata cgtaggtatt
      601 tggctagcaa atatagagct tgccaaacac tttagtgagt tttgtagaaa gtacctttaa
      661 aggtactttc acattacgaa cgatacgtcc gtaggacgta ctgaaagggt gggatttatg
      721 agcgaactaa tgcaaaattt ggctagcaaa aaaagttaaa aaatatatgc taataatcga
      781 gcgaaatgaa cactttttca agtgagcgaa tccgccatga caaagctgaa gcagtttatt
      841 tcagctttgt catggcgtgt gcctagactt ttaaaaaagt cggcacacga gcaaatatct
      901 ttatcaagct ctaatttgcg ttttaagacg tttgaaacgt ctaagtatat aaatatataa
      961 gagtaggttt aagagtgctt aaatcgaatt ttagagggca ttcagacaac tttagggttg
     1021 ttagggcttg ccctgaccgc ctgtaaggcg ctttaagaac aagataaggg atttatacta
     1081 agactgtctt aaaacgtctc aaacgaaggc tcatagcttc tcaaagagtt ccttatgaac
     1141 atttttagca aaggagccgg aagcgactgc gcagtataat gtgagagcag agcgatcaca
     1201 cctttttaag cgactgtagg gagtaagatt cccagttgag aaaaatgcgc gaatctgttt
     1261 ggagaaaaaa gaagtggttt tttaggcggg aaattttgtc cccctctttt ataatctctt
     1321 tttaaacctc tttttaaacc tctttaccgt cggctatatc cttactctcc caagggaatg
     1381 acgcttttat ctacatcgtt ttgtctgttt atctacatcg ttttgtctgt ttatctacat
     1441 cgttttgtct gtttatctac atcgttttgt ctgtttgtac attgaattag tacatcgacg
     1501 atgttaatat aagatgtaga taaacaggca ttttgtattt cataacgtgt atctcttgtg
     1561 cgagtaagaa aggagctatt ttaaatatgt ctaatgaaat cgtaaagtat catcatgaat
     1621 taaatacaat tccgttgcga aaatttacac cagtcgaaat gaatttgttc ttttctatcg
     1681 tatcaagaat gcgtgatgta ggtgataaaa aagtacaatt tacattcgag caattaaaag
     1741 atttaagcaa ttataaagca actgcaaatg ttcgattcat agatgattta gaaacaacgt
     1801 atgacaaatt aatggattta cgttttggta gaagaagtgc tgacggtttg caacgtgaac
     1861 gttttgtttt atttaatcaa tttaaaatcg acggaaaatc tgatattcct tttgctgaaa
     1921 tacaagttca tgaaaaagcc ttacccttac ttaataatct tgaagaatgg gtgcgctatt
     1981 ctctccaaca attcaatgaa ttagaaagca gttattcaaa aacaatgttt cgattactaa
     2041 aacaatacag aaccaaaggt ttcgcttatt tttcaaaaga agactttcat gaattacttg
     2101 atattcctaa aagttacaac caaggtacga ttgatcaacg tgtgttcaat ccaatacgac
     2161 aagaactaac cgcaatcttt aaaggtttaa caatcaaaaa gaaatacgga aaaggacggg
     2221 gaaagcctgt cattggctat caatttacat ttaaacctga gataaaaaaa tcagacgact
     2281 ttaacaaaca tgccccaaca aaagaagaac aaaacaaagc attagaacta gctaaccaac
     2341 aagttcaatc tgaaaaagca cctacttcat tacaagaaaa aatcaaagaa caaaagcgaa
     2401 aattggaact tttacaacag ttagaaaagt tagaaagaga acaaaatacc caaaataaag
     2461 aaaataatgc acaaaaatca gagataaatt ccgatattcc ttcagaacta aaaaaagact
     2521 tattagacaa tcttcaaaac ttattcaaag attgaggggt ttttagattt tatgacagaa
     2581 aaaacaatca aagaacttgc tgaagaatta ggagttagta aaacagcaat taacaaaaaa
     2641 gtttctgacg aaaatcgaaa actttggttt tcgaaaatcg gaaacaagtt tgtgattaat
     2701 gaaaccggac aaaaggctat aaaatcaatg ttttttaacg aaaacgaaaa ctcaaatcaa
     2761 caactagttg gcgaaaaaac gaaaaccgaa aaccacaaaa acgccaacca agttcccgaa
     2821 ttagaagtcc tttctctcct agaacaacag ttaaatcaaa aagatatgca actacaagaa
     2881 aaagacgagc aaataaaaca aatgcaaaaa ttactcgacc accaacaaca actcaccctg
     2941 caagccaacc aacaaatcga gaaactgcag gaacaactac agttgactta tacggaagaa
     3001 tcttcggaaa ataaatcaac gactctttct gaagaaacag aatcgataga aaaacaatca
     3061 aaggcggaga aaaaatggtg gcaattctgg caataaaacc agcaatttaa attaataaaa
     3121 attgctggtt ttattcttct ttttgctttt gcgagcagag caacatggac ccatgttgcg
     3181 atattttggt ggcgccccaa tccccgtttt ttcgttgaat cttactcgga ctgcactcgt
     3241 tttacagctg atttttctga attttgaaag agcataagga aacgtggtat cactttctta
     3301 cgaaaggtgc tagataggct gtgcctatca cgtttctcag aaaaaaattt gctatcactt
     3361 ttttgccatt gtggctaggc tatgtatagt gttttggcga aaaatagcta tacaaatact
     3421 atatttgtat ggtatacata aattatacgc tgatacgaaa ggaaaatttc catggacatt
     3481 ttagttcgaa atatcaatcc caaaattatt gcaagaatag ataaacgttg tcaggaactc
     3541 acaaccaaaa cgggcaaaaa atggagtcga aataaatatc taaaagttct aatcgaaagt
     3601 gattttgacc gtcaacttat gaattacaaa aaagatcaat tcgatttatt actcgaaaaa
     3661 tttattgcca ttcaaacaga aaatacgaac atactgaatg actatgttca cacaaacaac
     3721 caactcattg aaacacttat cggaggaata taacatgaga aaaacttctg ttcaaaaaca
     3781 cgtaaccctt tatcccagtc acgttgacgt tttagattcc atcatcgaaa aaaatccaga
     3841 cgtcaacaat tattcagaag ccattcgttt tttatgtatg agttatgaac ctgataccaa
     3901 tacaaccaca accagtaacc aagcaaaact caatgccatg ggaaaagaaa tttctatcat
     3961 gacagaaatc atggcagaat ttgctgattt atatctaaaa gatactggtg ttcttatcgg
     4021 tacaaattct aaagtgtatc aagaagtgaa agaaaaaatt gaaaatcaac tcaatccttc
     4081 ccgacgaaat caacccaaaa atcgtcaaaa agaaaccaat caaaccttta cttctggaaa
     4141 ttttgaccca acaaactttc taaaaagata aaaaaagaaa ccgatcccta aaaaaattcc
     4201 tcgatttctt tttagggatc agtcttccag caagatttgc aagttggtac caacttgcga
     4261 aaaaaaagaa aaatccgctt atgcaacttc ttctttttgt attttggggc gctaccccaa
     4321 tccccaagta tcggttattt aatagcgcgg aaatccaaag aataggacgt accctgaaac
     4381 aaaaaaagac tgctctcacg agcagcccaa aatgagatta tcctattcat caacataaaa
     4441 atattacaaa gtgaagaact agagaaagac aaaaaaagca tatcaaatcc tctcaaaaat
     4501 aacaagtctt taaaaaatga cagataaaaa tgcacaaaga actaaaaaag agaaatcgcg
     4561 taaaatcaag cattagaagc tagtaactag ctagaccaat taaaaatcac aaagtgagcg
     4621 tctctatcca ttggtagagg ggctacttgg catagtgtac attatgttaa ctaacgctat
     4681 aaataacgat tatgttccca aaacaatatt ttatgaacta taacaaaaat ttgttatcgc
     4741 atattgtata ttttaattat aggtgtatac cacttaaatt taacacaaag gcgtgattat
     4801 tttgaaaccc aaaaagaaaa tttatctagg tctcctcttt tccttgatga ttggaatttt
     4861 tagtatagga attacaaata cctatgcttc agaaaattct aatttcaata tcaactccca
     4921 accaattgaa gaacaagcaa ttaattttgc gaaaactgat tttgaacact ccataacttc
     4981 aattctttta atggaaaata acaccgaaac tgatattaac tatactcttg gttcgccatt
     5041 taaaatgctc gatggagaaa atacggcaaa tagttctatt ttctttccag tattttcaga
     5101 cgctgataat acgattaaat atatttatac cgtcaaaaaa aatacaaatg gagaatattc
     5161 aggaagtatt tcacaatttt tagcaaaaga acttcaaaat ctagtagata gtaatataac
     5221 agatacaacc ttattttttc aagataatag catttattat tccgataatg gacaaattaa
     5281 actactttgg aaatcacctt taagtacaga aaaaaatggt gtttcttcct ttacagaaaa
     5341 aaatgatctt atatccgtaa atataaaaaa taaaattaaa aatttctcta aaaaacaaaa
     5401 ttttcctgcg gttaaacgtg taaaaagtgt ctcctccgga gataacttta ttatgattaa
     5461 ttggcgtata gcagaaaccc aaacaagttt tccgtggtgt gctgcttacg tagaagctgc
     5521 tatcttatgt aacaaaacag atgtacgtgc tacttcagct agagccatta tgcattatac
     5581 ctatcctaat ttaaacaaca gtcaaattga atcaaaaagt cttaatcaag atcaaataat
     5641 aaaatatgca aattactgtg gtgtttatcc taaaaaagta gcacgaatac taaacctttc
     5701 agaggtccaa acacaactaa gaaaaggaaa tgccatttat atgggaactg ctggttatgg
     5761 aaattatagt aacagccgtc atgcacttgc attatttggg tgggtcaatc aaggtggtta
     5821 tcaaacttac tatgtgtgga atccttggta taactaccca actgtaataa gtgcaaatgc
     5881 aggaccagtt acaattcctg tacctggagg tggttttact tgggacaat
//
