LOCUS       AP031250                6151 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE12 plasmid pNUITMV12_6 DNA, complete
            sequence.
ACCESSION   AP031250
VERSION     AP031250.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639541
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 160x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE12"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV12_6"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(37..324)
                     /locus_tag="NUITMVRE12_31630"
     CDS             complement(37..324)
                     /locus_tag="NUITMVRE12_31630"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00771.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            complement(344..568)
                     /locus_tag="NUITMVRE12_31640"
     CDS             complement(344..568)
                     /locus_tag="NUITMVRE12_31640"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00772.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     gene            complement(1017..1187)
                     /locus_tag="NUITMVRE12_31650"
     CDS             complement(1017..1187)
                     /locus_tag="NUITMVRE12_31650"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00773.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1203..1736)
                     /locus_tag="NUITMVRE12_31660"
     CDS             complement(1203..1736)
                     /locus_tag="NUITMVRE12_31660"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFH00774.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(1757..2701)
                     /locus_tag="NUITMVRE12_31670"
     CDS             complement(1757..2701)
                     /locus_tag="NUITMVRE12_31670"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFH00775.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKEALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            complement(3545..4234)
                     /locus_tag="NUITMVRE12_31680"
     CDS             complement(3545..4234)
                     /locus_tag="NUITMVRE12_31680"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00776.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4241..4531)
                     /locus_tag="NUITMVRE12_31690"
     CDS             complement(4241..4531)
                     /locus_tag="NUITMVRE12_31690"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00777.1"
                     /translation="MTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTT
                     IDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(4479..5276)
                     /locus_tag="NUITMVRE12_31700"
     CDS             complement(4479..5276)
                     /locus_tag="NUITMVRE12_31700"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="BFH00778.1"
                     /translation="MKQIRMLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIA
                     PDHQIAIYTHTDKDHIHNHIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTI
                     TERGKKPEKRTMSEIKLKEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHC
                     LNRGKNFTYELIHKKKKVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIR
                     ETAKEVRREQRTLTTEQPEIRPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5410..5799)
                     /gene="mobC_2"
                     /locus_tag="NUITMVRE12_31710"
     CDS             complement(5410..5799)
                     /gene="mobC_2"
                     /locus_tag="NUITMVRE12_31710"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002498952.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="BFH00779.1"
                     /translation="MGLMSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     DDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_2"
     BGC             1..5568
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1758..2701)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.894"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4483..5381)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.333"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gattcttcag tcccattttc attttattcc aaagaattaa ctccaaatac caatagaagc
       61 ccatcctcta tatacttgag attccttact aacaatttct tgtaattcta ttaaagattt
      121 aggtaatcca aatttatgtt ttaaaatata tcttccaaga aaactactta atttcatact
      181 aacttcacca acactttttc cctcactgat ttctttagaa taaagaaaga gcttttcttt
      241 taagtcagga tagtcatcta tagttgcttc attgtatact ttactaattg catttaaaag
      301 tttttcttct ttagtaaaat ccattataat aacctcccta ccactacctt ctaggtgccc
      361 atggaccatg attaacccaa ccattaacaa taatttttcc aatttctcct ttagcttgat
      421 tccaatctac ccaacatttt tctttgttac aataaagacc atttccatag taagtagctg
      481 catcaacttt tattcctgta ccgatgccag ctagacaagt tcctaatatt cctagaataa
      541 cacaatgttt taatactttc tttttcatga ttgtttttcc ttttcgtttt tttaaaattg
      601 cttgttatat taaataatct tagtaatttt attatataac attttattat ataacaatga
      661 ttaacgtcat aaaaagtagt tttgaattca taatcgttta aataaaatta taagatcagt
      721 tatttaaaca gctagtagac ataatgtaca ctatgccaag tagcccttgt atcaaccgat
      781 acagaggctc actttgtgat tttcgattgg tctaggtcaa aagctctttt taagcttgat
      841 tttctgcggt tgcttctttc cgtttctttg tgaattttta ccagtcattt tttactgggt
      901 tgttattttt aataaaatat gataaatttt tggtagcgct tcataagcgc gttgcttttt
      961 caagaataaa cactcattat tttttaactg gctgctcgaa agagcagtct tttttattat
     1021 ttatctgttt cttttacaaa caattcttcg ttaggactgt tgtaataatt tggattcaat
     1081 ttcaattcac catgtttatt ccctttattg atcactgaca ataacatcgg actgaatcct
     1141 agaattccta gtaaaataga accgaataga agattttttc ttgtcatgaa tgttccctcc
     1201 agttatttcc aaagttgcca ccattttttt tcagtatcaa tttgtttgtc tattgtttct
     1261 gttgcttcag aaagagtcgt tgagtcattc tctggagctt cttctgtata agtcaactgt
     1321 agtttttctt gcagtttttc gatttgtttg ttggcttgta gggtgagttg ttgttgctgg
     1381 tcgagtagtt tttgtatatg agaattattt tgctctctga gggcaagttg ttctcgtaaa
     1441 aattcatttt cttcacgcaa ctgatctagc aacttgtttg taaccacgtt tttgcttggt
     1501 atattaacat ttttatcgtt gtcttgttcg tactcgtgca acctatctag caacatttgg
     1561 ttgcctaagt tgcgagcaag ttgcaatact ccagaacgca atacaacctt ataaccacgc
     1621 tcatttttag ctatcatgga agaatctaat ttttggatat gtttcgatat tgcttgccta
     1681 ctcatcttaa aaagttctga caattccttt attgtaaagg tttcttgtgg ttgcatactg
     1741 gttgcctcct aggttgctac tggaataaat tatctaaatc agataataaa ctttttttct
     1801 ctttttccga aagtgcggac tctgtttgtt tttcaagagc ttctttctct ttttctagtt
     1861 gtttgatttg ttggaggagt tcaagttctc gtttcttctg tttgattttt tcgttaattg
     1921 ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa agccaatgct ttattttggt
     1981 cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt tttgatctca ggtttgaacg
     2041 taaattgata gccaataaca ggttttcccc gtccttttcc gtatttcttt ttgattgtta
     2101 gtcctttgaa gattgcagtt agttcttgac gtataggttt tataactcgt ttatctatat
     2161 caggttgctt ataactttta ggaatatcta ataattcaag aaagtcttct ttggagaaat
     2221 atgcgaaacc tttggttctg tattgcttta acagacgaaa catagtcttt gcatagccac
     2281 tttgtagctc gttgaattgt tgtaaagaat agcgtaccca ctcgtctaaa ttatttaaca
     2341 aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg attttctacg tccccgttga
     2401 ttttaaattc tgtaaacatt acaaacatat cgtaagaaag cccagactca cttctacgac
     2461 caaagcgtaa agataagatt ttttcataag ttgcttttaa atcttctaca aatcgtacat
     2521 ttgcagttgc tttgtagtta cttaaatctt ttagttggtc aaacgtaaaa cgaactgtct
     2581 tatccccttt ttcacgcatt ctagacacga tggaaaaaaa caaattcatt tcaaccggtg
     2641 taaattttct taaaggaatt gtatttaatt catggtggta ttttacaatc tcattagcca
     2701 tataatcacc tcaatccaat tatgaatgac attttttaaa tgtcaagaca ttcatatttg
     2761 acgttcaaac agacaaaatg tccttcataa acagacaaaa tgtccttcat aaacagacaa
     2821 aatgtccttc ataaacagac aaaatgtcct tcataacatt ctgtatccat tggtagagta
     2881 aggacacagc ttacggtaaa gaggtttaaa aagaggttta aaaagagata taaaaagagg
     2941 ggggctaatt ttcccgccta aaaaccactt cttttttctt taaacaggtt cgcgcatttt
     3001 tcaaaactgc caatccaaaa ccttgcttcc tacggtcgcc aaaaaggtgt gatcgctacg
     3061 ctctcacatt gtactgcgca gtcgcctaaa ggctcctttg ctaaaaacat gttggtaaag
     3121 aggcctttga gcggttctga atgcttatct aagccgtttt aagacagtct tggataaaaa
     3181 tccttatcta gctttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaaa
     3241 taagcgatac ttaaaaacac cacacaagtt taatttttta agatataata acttgtcgcc
     3301 tatccgacag tcttgcgact ggaaggaggt gtatctaatg tttgaattgt tcatttgccc
     3361 ccttcttata gaagtttgtg tctctttatt cagttattgg ttgaataacc gaaacaagaa
     3421 gtaaaatcaa taaaggcgac atcaacctga aaaaaagccc cagagaatcc gacctctctg
     3481 gggctttcgt gtatctaatg tttgatcatt tgcatgttta gtttaacatg attgccgatc
     3541 aaattcaatt ttgctgttgc tgatctaacc attccttgac actttttaga taatttaatg
     3601 ccgtttcttt tttcgttgga ttttgtatgt tcgttgcttg ctgaagcagt gctttataac
     3661 gttcgtaggt aactgattgt tgccgttctt ttcgttcctg ttcactagtt gaaaaaggga
     3721 ttgtatcact gaataaatta taacaagctt cttctgtctg cgtatcttta aaatattgca
     3781 cgtctttttt ggttcgctca tagttgtctt gaacctcttt ttgctgtttt tctggcaagt
     3841 tttgataaag acgagtgagt ttttccatgt ttttatccgt caaacgtttt tcttgattca
     3901 caaaaatatc gttcatttcc tcataacaag tggtaaactc ttccattttt tgtgttaaca
     3961 cttgctcctt tttcttttta tcacatgctg cttcatactt ttgagtgata tcccctaata
     4021 aaatacttct tgaaatacgt ttatttccct ctaaaacgcc gaataaacca cttaaaacga
     4081 ttgataatgt aaatataacc cctggcaata aaaacctctt aaatcgaatt ttagagcctt
     4141 ttaatgaata tcttgtttga taagccttat agctaagcca aataattcct aaaatactgg
     4201 caaaaatgag tagatagggt aaatagaatg ccatttctat ttattctccc cataccagcc
     4261 aaaaggctaa cagtgtcatg attaagcaac ataagggggc aagaatatcc attgatcgct
     4321 gcataattac ttttttctgt tctttatttc gctggtttaa tagcgcatgg tggaattttt
     4381 tgacttgtga atcaattgta gtttgtattc tttctttggt ataaggtgca agaaaagcac
     4441 gttgtttctt tatttcattt tctatgtctt gtaacgtttt agaacgataa ttcttgttgg
     4501 tagtctgctc ctctttcaac gtgttggtca tggtttcttc gagtcggtct aatttccggt
     4561 tgttcagtcg taagagttcg ttgttctctt cgtacttctt ttgcagtttc gcgaatgcta
     4621 gctcgagctc ttcgtagctc gttggttcgt tgttgattgt aggctcgttc tcgtctgtcc
     4681 agctcatgta aaatcgtctc cttttcgtaa tcctttccta gtttgttgga tcgtactttt
     4741 ttctttttgt ggatcaattc gtaagtaaaa tttttcccac gattcaagca atgaatgccg
     4801 aatgttttaa gagaatcaca aaattggtta tacgttcgag tttttttgtt tttcattgtt
     4861 tgatcaatcg caaaccgaat ttcgtctttc caaacaggtt catttttttc ttttaatttg
     4921 atttcagaca tggttcgttt ttcgggtttc ttccctcgtt cagtgattgt taagtcatgt
     4981 tcaagacaaa tctcgtcatt taggtcataa catttgttta aaaatgcttg accatgggct
     5041 tgaaatttgt tcccagtttc cagattgacg gagttaatga caatgtggtt atgaatatgg
     5101 tctttgtctg tatgtgtgta aatcgcgatt tggtggtcag gggctatttt tttcgcatac
     5161 tctgccccta gttgattggc tttttcagcg gtaatttctc ctggtcgaaa agactggatc
     5221 aataagtgtg cttgtacacg atcgtctttt ccatacagca tgcgaatttg tttcatttcg
     5281 cttttggcat aatcaatgtc acagtttatc cctgttttga cggttgctcg tggttcggcg
     5341 taattgatcg ctcggctaca agaagtggtt cggcgtaatt gatcgctcgg ctacaagaag
     5401 tggttcggct tactttaact gttgccatat cttttgtagc tccttttgca tgatttttag
     5461 gttttcggat aaacgttgag catcatcagg agcaacgtcg tgttggtgtg tattgcacca
     5521 cttggcaatt tgattgatat tccctccagc tttggctaaa tcgcgcgcga ttgcctgggc
     5581 aatgtctggc gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg ctttcacgaa
     5641 agccggcaca ctcatttgca aagcttcagc tgactgcttc agctttgcaa attcggattc
     5701 gctcactcga aaactgattt gttttggctc ggatcgatag gtatattttt tagaatgatc
     5761 gctagccaaa ttttgatgtt gttcgctcat aagtcccacc ctttcagtac gtcctacgga
     5821 cgtatcgttc gcaataccat tgtacctaaa aaggtacaat gagtaaagct cactaaaagg
     5881 gtttggcaag ctctatattt gctagccaaa tacctccgta tttggtgcaa ataaccgctt
     5941 gtcaaaggag cgcaagctcc cccttgaatc cccctcaaaa tccacgctcc acaatacaaa
     6001 aaaaggctag catttgctag ccactttttg taaagtttcg cttatttcac cgaaggtgaa
     6061 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     6121 gccatttact attaataaat ctataaatat g
//
