LOCUS       AP031249                6298 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE12 plasmid pNUITMV12_5 DNA, complete
            sequence.
ACCESSION   AP031249
VERSION     AP031249.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639541
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 160x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE12"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV12_5"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(244..534)
                     /locus_tag="NUITMVRE12_31550"
     CDS             complement(244..534)
                     /locus_tag="NUITMVRE12_31550"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00763.1"
                     /translation="MNNTLNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTT
                     MDSQVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(482..1396)
                     /locus_tag="NUITMVRE12_31560"
     CDS             complement(482..1396)
                     /locus_tag="NUITMVRE12_31560"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="BFH00764.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1378..1767)
                     /gene="mobC_1"
                     /locus_tag="NUITMVRE12_31570"
     CDS             complement(1378..1767)
                     /gene="mobC_1"
                     /locus_tag="NUITMVRE12_31570"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002498952.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="BFH00765.1"
                     /translation="MGLMSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     DDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_1"
     gene            complement(2080..2277)
                     /locus_tag="NUITMVRE12_31580"
     CDS             complement(2080..2277)
                     /locus_tag="NUITMVRE12_31580"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00766.1"
                     /translation="MISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQLYK
                     SGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(2295..2828)
                     /locus_tag="NUITMVRE12_31590"
     CDS             complement(2295..2828)
                     /locus_tag="NUITMVRE12_31590"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00767.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(3278..3448)
                     /locus_tag="NUITMVRE12_31600"
     CDS             complement(3278..3448)
                     /locus_tag="NUITMVRE12_31600"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00768.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(3511..4002)
                     /locus_tag="NUITMVRE12_31610"
     CDS             complement(3511..4002)
                     /locus_tag="NUITMVRE12_31610"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFH00769.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETKQ
                     "
                     /name="DUF536 domain-containing protein"
     gene            complement(4023..4973)
                     /locus_tag="NUITMVRE12_31620"
     CDS             complement(4023..4973)
                     /locus_tag="NUITMVRE12_31620"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFH00770.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     Typing          complement(4024..4972)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.057"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(486..1396)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttttgagtg atatccccta ataaaatgct tcttgaaaca cgtttattac cctctaaaac
       61 gccgaataaa ccacttaaaa cgattgataa ggcaaatata accccgacca ataaaaatcg
      121 cttaaatcga attttagagc cttttaatga atatcttgtt tgataagcct tatagctaag
      181 ccaaataatt cctaaaatac tggcagaaat gagtagatag ggtaaataga atgccatgtc
      241 tgtttattct ccccatacca accaaaaggc taataatgtt agtactaagc aacataaggg
      301 ggcaagaata tccatcgatc gttgaattaa agcctttttt tgttcttggt ttcgctggtc
      361 taatagcgct tggtggagtt ttttgacttg tgaatccatc gtagtttgta tcctttcttg
      421 ggtataaggc gcaagaaagg cacgttgtct ctttatttcg ttttcgatgt cttttaacgt
      481 tttagagcga taattcttgt tggtggtctg ttcctcgttc aacgtgttgt tcatggtttc
      541 ttcgagtcgg tctaatttcc gattgttcag gcgtaaaagt tcgttgttct cttcgtactt
      601 cttctgcagt ttcgcgaatg ctagctcgag ctcttcgtag ctcgttggtt cgttgttgat
      661 tgtaggttcg ttctcgtctg tccagctcat gtaaaatcgt ctccttttcg taatcctttc
      721 ctagtttgtt ggatcgtact tttttctttt tgtggaccaa ttcgtaagta aaattttttc
      781 cgcgattcaa gcaatgaatg ccgaatgttt taagtgaatc acaaaattgg gtatacgttc
      841 gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg aatttcgtct ttccaaaccg
      901 gttcattttt ttcttttaat ttaatttcag acatggttcg tttttcggtt ttctttcctc
      961 gttccgtgat tgttaagtca tgttcaagac aaatctcgtc atttaggtca taacatttgt
     1021 ttaaaaatgc ttgcccatgg gcttgaaatt ttttcccagt ttcaagatta acggagttaa
     1081 tgacaatgtg attatgaata tggtctttgt ctgtatgtgt gtaaatcgcg atttggtggt
     1141 taggggctat ttttttcgca tactctgccc ctagttgatt ggctttttca gcggtaattt
     1201 ctcctggtcg aaaggactga atcaataagt gtgcttgtac acgatcgtct tttccataaa
     1261 gcgtgcgtat ttgtttcatt tcacttttgg cataatcaat gtcacagttt attcctgttt
     1321 tgacggttgc tcgtggttct gcgtaattga ttgctcggct acatgaagtg gttcgactta
     1381 ctttaactgt tgccatatct tttgtagctc cttttgcatg atttttaggt tttcggataa
     1441 acgttgagca tcatcaggag caacgtcgtg ttggtgtgta ttgcaccact tggcaatttg
     1501 attgatattc cctccagctt tggctaaatc gcgcgcgatt gcctgggcaa tgtctggcgc
     1561 cactttcggt gtcaccagac gcgcgttttg tgccttggct ttcacgaaag ccggcacact
     1621 catttgcaaa gcttcagctg actgcttcag ctttgcaaat tcggattcgc tcactcgaaa
     1681 actgatttgt tttggctcgg atcgataggt atatttttta gaatgatcgc tagccaaatt
     1741 ttgatgttgt tcgctcataa gtcccaccct ttcagtacgt cctacggacg tatcgttcgc
     1801 aataccattg tacctaaaaa ggtacaatga gtaaagctca ctaaaagggt ttggcaagct
     1861 ctatatttgc tagccaaata cctccgtatt tggtgcaaat aaccgcttgt caaaggagcg
     1921 caagctcccc cttgaatccc cctcaaaatc cacgctccac aatacaaaaa aaggctagca
     1981 tttgctagcc actttttgta aagtttcgct tatttcaccg aaggtgaaat tgttgctttg
     2041 taatattcaa acaaccctat tcttctaaaa tgtatcgatt tatttttttt tagactttat
     2101 tatgtctaag acaactaaga ttaacactaa aaataaacca gatttgtata attgccctcc
     2161 gcttagcata ttaacaatta tagcaacagc tatagcaaat ataaaaatac gaatagtagc
     2221 tatttgcgga ttctttttag taaatgtttt taatgatttt aatgatttag atatcaaaaa
     2281 catcactcct taacttataa cgttgaatat ctagtagcta caactcttcc agctgcatca
     2341 tatccccttt ggtaataaaa tccaatccag ccattaccat ttttgtccca ttcttttgca
     2401 actgcattcc ctggagaagc acctttttta gtccaacttg cacctgttaa caaagcacca
     2461 attgcccaac ctgggatacc aaacatagaa gtgctagctt tccctcctgc ccaaaaggca
     2521 gcattaatag cattagcagc tactccagtg gttacagcaa tatttgtata ttcctttttg
     2581 ccccatcttg caactgcagc tcttgtcatg atatctgaag aatttgcaaa atcttccgca
     2641 ctataaccat cctcatattt agttacgacc ataccatctt cagtgtactc aataactaca
     2701 ttttcagtag cattgttttc gtttaatgat accacttggg aattttctga agcataaact
     2761 atagtagttg acggtaaaac aactgcccca atagttaaca aagctacccc taaagtaaat
     2821 tttttcatag aatttcctcc tgatacagaa aaatatatag aaaacgtttt ctttgtgaaa
     2881 atcataaaac aaaaggtata ggcttgtcaa tattttattt agtaaagata atttatattt
     2941 ttgataatgt ggtatagtta gaatgcatta ggaaaatatg ttatattaaa ttcttgttga
     3001 cataatgtac actatgccaa gtagcccttg tatcaaccga tacagaggct cactttgtga
     3061 ttttcgattg gtctaggtca aaagctcttt ttaagcttga ttttctgcgg ttgcttcttt
     3121 ccgtttcttt gtgaattttt accagtcatt ttttactggg ttgttatttt taataaaata
     3181 tgataaattt ttggtagcgc ttcataagcg cgttgctttt tcaagaataa acactcatta
     3241 ttttttaact ggctgctcga aagagcagtc ttttttatta tttatctgtt tcttttacaa
     3301 acaattcttc gttaggactg ttgtaataat ttggattcaa tttcaattca ccatgtttat
     3361 tccctttatt gatcactgac aataacatcg gactgaatcc tagaattcct agtaaaatag
     3421 aaccgaatag aagatttttt cttgtcatga atgttccctc cagttatttc caaagttgcc
     3481 accatttttt ttcagtatca atttgtttgt ctattgtttt gtttctgttg cttcagaaag
     3541 agtcgttgag tcattctctg gagcttcttc tgtataagtc aactgtagtt tttcttgcag
     3601 tttttcgatt tgtttgttgg cttgtagggt gagttgttgt tgctggtcga gtagtttttg
     3661 tatatgagaa ttattttgct ctctgagggc aagttgttct cgtaaaaatt cattttcttc
     3721 acgcaactga tctagcaact tgtttgtaac cacgtttttg cttggtatat taacattttt
     3781 atcgttgtct tgttcgtact cgtgcaacct atctagcaac atttggttgc ctaagttgcg
     3841 agcaagttgc aatactccag aacgcaatac aaccttataa ccacgctcat ttttagctat
     3901 catggaagaa tctaattttt ggatatgttt cgatattgct tgcctactca tcttaaaaag
     3961 ttctgacaat tcctttattg taaaggtttc ttgtggttgc atactggttg cctcctaggt
     4021 tgctactgga ataaattatc taaatcagat aataaacttt ttttctcttt tttcgaaagt
     4081 gcggactctg tttgtttttc aagagcttct ttctcttttt ctagttgttt gatttgttgg
     4141 aggagttcaa gttctcgttt cttctgtttg attttttcgt taattgttgt agcaggttct
     4201 tcgtattctt tttgtttatc agctaaagcc aatgctttat tttggtcttc tttggttggt
     4261 gcatgtttat taaaatcatc tgcttttttg atttctggtt taaacgtaaa ttgatagcca
     4321 acgacaggtt ttcctcgccc ttttccgtat ttctttttga ttgataatcc tttgaagatt
     4381 gcggttaatt cctgtcgtat aggctttata acacgattat caacgtttgc ttggttgttc
     4441 cagtaacttt ttggaatatc taatagttca tgaaaatcct ctttagaaaa ataagcaaaa
     4501 cctttagttc gatattgttt tagtaatcga aacatagtct ttgcatagct actttgtaat
     4561 tcgttaaatt gttggagtga atagcgaacc cactcatcta aattgtttag taaaggtaaa
     4621 gctttttcgt aaacttgtat gtctacatat ggatctttag cagttccacg tattttaaac
     4681 tcactaaaca taacgaatct atctatatcg agcccattag cacttctttt accaaaacgt
     4741 aaatccatta atttatcata agtcatttct aaatcattaa tgaaacgatt attagcagtt
     4801 gctttataat tacttaaatc ttttagttgg tcaaaagtaa gacggactgt tttatcgcct
     4861 ttttcgcgca ttctagacac aatagagaaa aatagattca tttcaactgg tgtaaatttc
     4921 cgcaacggaa tggtatttaa ttcatgatgg tatttcacga tttcatttgc caaagatatt
     4981 cctccttact ccttcaatgg atacagacgt tataaacaac gttttgtctg tgataatgca
     5041 tattataaca tacgtgagtg caaaaaaaga gtttaaaagc agacaaaatg tagtttaaaa
     5101 gcagacaaaa tgtagtttaa aagcagacaa aatgtagttt aaaagcagac aaaatgtagt
     5161 ttataaaacg ctgtatccat tgaaggagta aggacacagc cgacggtaaa gaggtttaaa
     5221 aaaaggttta aaaagagatt ataaaagagg gtgacaaaat ttcccgccta aaaaccactt
     5281 cttttttcta ccaacaggtt cgcgcatttt tcaaaactgc caattcaaaa ccttgctccc
     5341 tacggtcgct aaaaaagtgt gatcgctatg ctctcacatt gtactgcgca gtcgcctaaa
     5401 ggctcctttg ctaaaaacaa gtcagtaacg gaggtggggg aagcctctga tagctcgtct
     5461 aagtcgtttt aagacagtct tgggtaaaaa accttatctt gttcttaaag cgccttacag
     5521 gcggtcaggg caagccctaa caacccgaaa ataagggata cttaaaaaca ccacacagat
     5581 tttattttgt aaggtataat aacttgtcgc ctatccgaca gtcttgcgac tggaaggagg
     5641 tgtatctaat gtttgaacgg ttcatttgcc cccttcttgt agaagtttgt gtctctttat
     5701 tcagttattg gttgaataac cgaaacaaga agtaaaatca ataaaggcga catcaacctg
     5761 aaaaaaagcc ccagagaatc cgacctctct ggggctttcg tgtatctaat gtttgatcat
     5821 ttgcaggttc agtttaacat gattgctaat caaattcaat tttgttgttg ctgatccaac
     5881 cattccttga cactttctag ataatttaat gccgtttctt ttttcgttgg attttgtatg
     5941 tttgttgctt gctgaagcag tgtttcataa cgttcgtatg tgaccgattg ttgccgttct
     6001 tttcgttctt gttcactggt tgaaaaaggg attgtatcgc taaataagtt acaacaagct
     6061 tcttctgtct gcgtatcttt aaaatattgc acgtcttttt ttacttgctc atatttttct
     6121 tgtacctctt tttgctgttt ttctggtaag ttttgataaa aacgagtgag ttttttcatg
     6181 tttttatctg ttaaacgttt ctcttgatcc acaaaaatat tgttcatgtc ctcataacaa
     6241 gcggtaaatt cctccatttt ttgtgctaat gcttgctctt ttttcttttt atcacatg
//
