LOCUS       AP031248               23125 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE12 plasmid pNUITMV12_4 DNA, complete
            sequence.
ACCESSION   AP031248
VERSION     AP031248.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639541
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23125)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 160x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..23125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE12"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV12_4"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            626..1363
                     /gene="erm(B)_2"
                     /locus_tag="NUITMVRE12_31320"
     CDS             626..1363
                     /gene="erm(B)_2"
                     /locus_tag="NUITMVRE12_31320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002321849.1"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="BFH00741.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
                     /name="erm(B)_2"
     gene            complement(1760..2440)
                     /locus_tag="NUITMVRE12_31330"
     CDS             complement(1760..2440)
                     /locus_tag="NUITMVRE12_31330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001015311.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="BFH00742.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(2873..3085)
                     /locus_tag="NUITMVRE12_31340"
     CDS             complement(2873..3085)
                     /locus_tag="NUITMVRE12_31340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 2878"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00743.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            complement(3106..3957)
                     /locus_tag="NUITMVRE12_31350"
     CDS             complement(3106..3957)
                     /locus_tag="NUITMVRE12_31350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287227.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00744.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            complement(4058..4354)
                     /locus_tag="NUITMVRE12_31360"
     CDS             complement(4058..4354)
                     /locus_tag="NUITMVRE12_31360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000053907.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="BFH00745.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            complement(4489..5982)
                     /locus_tag="NUITMVRE12_31370"
     CDS             complement(4489..5982)
                     /locus_tag="NUITMVRE12_31370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000947691.1"
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="BFH00746.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            6594..7547
                     /locus_tag="NUITMVRE12_31380"
     CDS             6594..7547
                     /locus_tag="NUITMVRE12_31380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000429439.1"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="BFH00747.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            7519..7794
                     /gene="prgO"
                     /locus_tag="NUITMVRE12_31390"
     CDS             7519..7794
                     /gene="prgO"
                     /locus_tag="NUITMVRE12_31390"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001196543.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="BFH00748.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            8022..8702
                     /locus_tag="NUITMVRE12_31400"
     CDS             8022..8702
                     /locus_tag="NUITMVRE12_31400"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001015311.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="BFH00749.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            8946..9551
                     /locus_tag="NUITMVRE12_31410"
     CDS             8946..9551
                     /locus_tag="NUITMVRE12_31410"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000599739.1"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="BFH00750.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="Fic family protein"
     gene            9567..10139
                     /locus_tag="NUITMVRE12_31420"
     CDS             9567..10139
                     /locus_tag="NUITMVRE12_31420"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000170424.1"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="BFH00751.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(10572..11531)
                     /locus_tag="NUITMVRE12_31430"
     CDS             complement(10572..11531)
                     /locus_tag="NUITMVRE12_31430"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287225.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="BFH00752.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINFL
                     KEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPISCS
                     VRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEFGHI
                     EGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFKSITF
                     DCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQVDQAF
                     ISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
                     /name="IS30 family transposase"
     gene            complement(11659..12339)
                     /locus_tag="NUITMVRE12_31440"
     CDS             complement(11659..12339)
                     /locus_tag="NUITMVRE12_31440"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001015311.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="BFH00753.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            12429..13127
                     /locus_tag="NUITMVRE12_31450"
     CDS             12429..13127
                     /locus_tag="NUITMVRE12_31450"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00754.1"
                     /translation="MSTEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYV
                     ANRGNKYTFELAQSFSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTT
                     EFDDDTVQAIMDEALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYD
                     VTQHIPLSEAQAGDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQ
                     HLVGAGRIKQ"
                     /name="hypothetical protein"
     gene            13124..14056
                     /locus_tag="NUITMVRE12_31460"
     CDS             13124..14056
                     /locus_tag="NUITMVRE12_31460"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001224319.1"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="BFH00755.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVLL
                     GLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSIDN
                     RMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQLITE
                     GENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSITTNEI
                     NEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTVSLTVE
                     YIDQQTKATQVSQFDLVLEKNGSNWKIVK"
                     /name="conjugal transfer protein"
     gene            14433..16352
                     /gene="tet(M)"
                     /locus_tag="NUITMVRE12_31470"
     ARG             14434..16349
                     /name="tet(M)"
                     /gene="tet(M)"
                     /gene_name="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            16546..17922
                     /gene="tet(L)"
                     /locus_tag="NUITMVRE12_31480"
     ARG             16547..17919
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            18544..18828
                     /locus_tag="NUITMVRE12_31490"
     CDS             18544..18828
                     /locus_tag="NUITMVRE12_31490"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00758.1"
                     /translation="MVDYWNDCFNDLHILQPDWKTIERTSDRAMVFMLLNDEEEWGKLE
                     RRTKNKYKKLIKEISLIDLTDLMKSTLKANEKQLQKQIDFWQREFRFWK"
                     /name="hypothetical protein"
     gene            18963..19610
                     /gene="catA"
                     /locus_tag="NUITMVRE12_31500"
     ARG             18964..19607
                     /name="catA"
                     /gene="catA"
                     /gene_name="type A-8 chloramphenicol O-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     gene            19863..21074
                     /gene="mobV"
                     /locus_tag="NUITMVRE12_31510"
     CDS             19863..21074
                     /gene="mobV"
                     /locus_tag="NUITMVRE12_31510"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000122833.1"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="BFH00760.1"
                     /translation="MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNYD
                     LVNRTENYKRDIEQFINDNKSSSRAVRKDAVLINEWIITSDNPFFKDKDDKEIKDFFDT
                     AKSYFADKFGDNNIRYAQVHLDETTPHMHLGIVPFNDEHKLSAKTVFNRQALQAVQDEL
                     PKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEITTTLEQRKSEVLALSNDKAPT
                     IKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVIKSYEE
                     LYKANEKLKNYAETDLPKEITRVKEKYRELAKDYNRLVRKSNQNIETLEELEKENKSLK
                     NEIKGIYKGFNQFMENTLGATVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNETKTRDRD
                     ELSR"
                     /name="mobV"
     gene            21609..21773
                     /locus_tag="NUITMVRE12_31520"
     CDS             21609..21773
                     /locus_tag="NUITMVRE12_31520"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00761.1"
                     /translation="MHFVVEVEKKKVTLSLPVESNDKLEKMAQKYGMTKSGLVTFLINQ
                     ADDKGTIFK"
                     /name="hypothetical protein"
     gene            21897..22424
                     /locus_tag="NUITMVRE12_31530"
     CDS             21897..22424
                     /locus_tag="NUITMVRE12_31530"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH00762.1"
                     /translation="MENENRTDWTLPRKNLNPKTKQPYKRGRNWWIVVYPESLPENWKE
                     IISTEPVAISPLHDKDVNADGTKKKPHYHIVFNYKGNKSFEQMDEMARVLRAPIPERIS
                     GLTGAVRYLTHMDNPEKYQYDNTEIQVFGGFDLESCLALSTGDKRQALKEMLGFISDNN
                     IMHLKVLLQSFK"
                     /name="hypothetical protein"
     gene            complement(22454..>23125)
                     /locus_tag="NUITMVRE12_31540"
     misc_feature    complement(22454..>23125)
                     /locus_tag="NUITMVRE12_31540"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="partial CDS similar to WP_001015311.1:IS6-like
                     element IS1216 family transposase"
     ARG             627..1360
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             18964..19610
                     /name="catA8"
                     /gene="catA8"
                     /gene_name="chloramphenicol acetyltransferase (CAT)"
                     /tool="rgi"
                     /drug_class="phenicol antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             16637..17922
                     /name="tet(45)"
                     /gene="tet(45)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="76.35"
                     /coverage="93.45"
     ARG             627..1363
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.78"
                     /coverage="98.79"
     Typing          complement(4490..5982)
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          18545..18828
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.17e-147"
     Typing          19864..21071
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.841"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcctcctgc tatctttttc tattattcta ccttatttga tagtagattt aaaactttgc
       61 aacagaacct tttgtacatt taacttgacg gtgacatctc tctattgtga gttattagtg
      121 gtacagtttt caaccgtttt aattataaaa aagtggtgca tttttaaatt ggcacaaaca
      181 ggtaacggtt attgcaggtg tatttcttat ctatgggttt aacatggatt ttatcattaa
      241 aatcatgagt attgtccgag agtgattggt cttgcgtatg gttaacccta aagttatgga
      301 aataagactt agaagcaaac ttaagagtgt gttgatagtg cattaaaaat tttgtataat
      361 aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg
      421 ttggtattcc aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac
      481 agtgattacg cagataaata aatacgttag attaattcct accagtgact aatcttatga
      541 ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag
      601 atgtaatcac ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt
      661 aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac
      721 cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa
      781 acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa
      841 actgaatact cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa
      901 acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa ttattaaaaa
      961 agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa
     1021 gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca
     1081 gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt
     1141 aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta
     1201 ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca
     1261 tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt
     1321 gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt
     1381 gtaaatttgg aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc
     1441 acctctaata actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt
     1501 tttaaatcta tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg
     1561 aaatgttttt ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt
     1621 aaggtttaaa tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag
     1681 ctttccaatt tttgttgaat tgcttggttc tgttgcaaag ttttaaataa agaataaaat
     1741 cccttacggt atctatgatt taagctggga ttcccaataa taccttgatt tcagtacaga
     1801 ccgaaaaccc gaagagagtg ccttcttttc gggttttctt atataatcct cgaatggctt
     1861 ccatgccttt aatcgtggta gaggcagtgc gtaaacttcg atagaattta ttgcgtctct
     1921 ttactggacg atggtcttgt tcaatcaaat tattcaggta tttaatggta cgatgttctg
     1981 tcccttgata aaagccgtat tcttttagtt tcttaaaggc acttgtaata gagggggctt
     2041 tatctgtgac tacaaccttc ggttcatcaa actgcttcac taaccgctta agaaaagcat
     2101 aggctgcttg tgtgtcccgt tttttacgta accaaatatc caaggttaaa ccatctgcat
     2161 cgatggctcg atacaaataa tgccattttc ctttaatttt gatgtacgtt tcatccattt
     2221 tccatgaata aaaggatttt ttatttttct ttttccaaat ttgatagagt agtttgccat
     2281 attcttgcac ccaacgataa atcgtcgtat gagaaacgtt aatgccacga tcatataaga
     2341 tttcttgaac ttcacgatag ctaaggttat aacgaagata gtagcccacg gctacaataa
     2401 tcacatcctg ctgaaattgc tttcctttaa aatgattcat cgtcattcct cctgctatct
     2461 ttttctatta ttctacctta tttgatagta gatttaaaac tttgcaacag aaccgttcgt
     2521 cacatcttcg atcaatttct cttacgattt tctcatccac atttcttatt cttacttcca
     2581 tattaattcc tccaatttaa aaaggatagc gaaaaggagc ctatcctttt tgctatccta
     2641 ctaccctcct tttactatcc ttcactatcc tttcactatc ctatttttaa atattttgtt
     2701 cccacattta tttttgagaa acgtaatagg cacagcctat tggaacactt tttttaaaaa
     2761 gtgttcccac gtttccttat gctctttaca aataaaggct tttagagacg ttctggaatt
     2821 ttagattcct tgaattgttc ccattcatca atcaattggc tataagtagc cattaatttt
     2881 tttcttgctc ttgttgcttt cgaatcaaca aaaaatcagt ttgattcgtt aattctaaat
     2941 attctgttcg gtattcaatg ttcatcgtac ccgttttact acatagtaac tgattatgac
     3001 gttctttcac tttgaataac tgctccttct ttaaggtttt taactccatg ccaactataa
     3061 gcacaatcct gtatagagga atcattttca aacgattctc cataattatt tatctttcat
     3121 tggaagttct aaatcactac tgttccaaaa aatgtatggt ttgttttgac gaatattact
     3181 tccatctaac cacaattttc cgatgccata tcgtaggctt gcttcgactt cataaaaatt
     3241 gtagatctct tttcttcgat ctattgcttt catttcctgg ccatttttta aaatagatct
     3301 gatgccctta acagtagatt catggatact cgtgtgaacg atatccgtat tgatcaattc
     3361 gtcatatctt ccaaaatcaa cattgtactc ataaccaaga agtgtcggaa tcaggtcaat
     3421 ttcaatttct ttttcaattg aacgtttaat aagtttcgtg gatacatacc cgacaaaaaa
     3481 gagaataata cccacttcaa acaaatacat gtgtccattt acaaagtgaa taaacgtcaa
     3541 taaaaaatcc ttcactcctt ttgttgttct tccagattgt tcaatttgtt ttattaaagt
     3601 cgctagaatt gcatttacaa caattaaatc gaataggctt ttgaaaagtt ttcctgctgt
     3661 taatttttgc ctggtaaatt gcatgactaa ttgagtaaaa atagacaata gcatgacaaa
     3721 aatcaacgtc attagcactg taaaaatgtc tgatgctgca atcctattgg gatcatttat
     3781 caagtaataa gccgttccaa atactccaat cgagtaaacg gttaaatcaa gaataagtcg
     3841 tgccccgtaa ctacaaagtt ttcctaatgg ccataattgc aatacccaaa atttctttcg
     3901 tttgctattt cttttcagag gaagccaggt gtgttcaatc cagtcgtccg atttcatttc
     3961 gatttcttcc ctaaattctt tcctttaacg ttttcctttg ctactttagc agcgataggt
     4021 tgctcaatga accaattttt taagtaaata aaaactacta ctggatatac ttcttccttt
     4081 ttcttaactc gttagctatt ttatactcaa tatactcctg ctctaacaca cacaaacaat
     4141 catatacctg atccatagtc tccccagaag ctaaggacaa cccatgaaga aactcaatcg
     4201 gcaaagaagc aactgttttt tccctggtaa cccaagaaga caaagtcccc tgcttaaaac
     4261 cataaagctc acagaacact tccacagata atcccaaacg accaatgata aacacattaa
     4321 caggatgtga atagacataa ttttttaacg acatacaact tcctccatga tacataagac
     4381 tgcatacgca gttataagta taccacctac acgtagctag gcaaagggag caacacctac
     4441 agaagtatca tagatatatt gtatagggca atttgcacct tttatttttc aacctgtatc
     4501 tacttcaaaa agattcactt ctttaagagg gttcaagggg gttgataatt gtctaaatgt
     4561 ctcttctact aagctctcgt tgagtccaaa tacctctgtg atccttgttg aataatctag
     4621 tctcttttct ttacttagtt gaataaccct cgcgaacaac gttttgactg aagcaatgat
     4681 cattccgcca tttcttcctc gtgttacctt gtagaagact ttattctcct ctcgtaactc
     4741 atttaagatc ttatcaagtg ttcgcttggg catcgttagt tgtgaagcca attctttttt
     4801 tgacaggttc aaataaggct cactagcaag acttttttcg cttagataag ccaatagatc
     4861 ttctttccat tcatttttat gactatattt tctttgatct cgtggctttt taaatttcca
     4921 ccagccacgt cgttgaatga acagatcatt attttttaag gttggtgaaa cccattgctg
     4981 gcacaattca ataatgaatt ctctgcttgc tgcttgataa ttaccagaat aagcactacg
     5041 tacaatcttt actaattctg cttcagatag cggttcctgt aaccgttcgt tgaatgtgaa
     5101 catattgtat tcgcaagtat ccaatgcata acctgaagag taataagcta aagaaagcgt
     5161 gaaaatcaca ttatttcgac caagacgtcc ttctccgccg acgatgtttg ctttatgaag
     5221 taacatatcg aaccacggtt catctacttg acgaatttct tttttctcag gaaagacaat
     5281 tagttttcgt tctcgatctt cttttgtttt ttgatctgat tcaagcttca tcgaccattg
     5341 aatccagtca gcaaaagaat actgatagtt catttcttta aaaacaatat tattttcttg
     5401 tgggaatcga gcaattccga agtggttaca tcctagatcc actccggaca atttttcagc
     5461 caactgtaga cgaattgttt ttgaaatttt ttttgctaca tcgatcacct tgaaattgct
     5521 tttctttgtg acatagcctg gtgtctttag tacaaaataa acttgataac catgatctga
     5581 ttctaaaatt agcgttggca tgtagcccac ttgatctaaa caggctaaaa taatttcgcc
     5641 ttgatggttc tcttttgaat cgatatcaac tacaaatgtg ttgatttgac ttaagttttt
     5701 ctcagaatga cctttaacca ctgttcgata atcatccgca taagtaccaa agcgataggt
     5761 attaggtgtc caatgcgtta gtttcttttc gttctctaaa atagcttctt ccgaagtaat
     5821 gaccatacca cgcccaacat tcattaattg cttgctacgg tacgcaaaga tagcaccact
     5881 cctactggaa tcaatatgac ctgcaggttt catggtgctg tttcgatatt tgtatgtacg
     5941 taagccgttt ttaagaatag ctgaataaat ttcgctcaac atcgattgta acgtcctttc
     6001 tcaaacaaaa agagaacaca gtttagcttt aatctcagcc atagtatgtg ttctctcttc
     6061 agtgtttaag aatatttaat ttaagtacac taaataaact ttcttctgtt gagttgctcg
     6121 cctttttttg gtagaataaa ggcaatcgga aaaggtatcc gatcaacttt taatataagt
     6181 agttggtagc tacttacata ttcttgagac gagcgtgaac acatgtccta accatgtgat
     6241 tcagaaataa ttgattctgc taaaatcaat tatttccgtc tctttttttt atgtaatttt
     6301 tctgtttaaa gagtaccact gcttttgcta ctttgtaaat agtgaattat ctgtggtttc
     6361 tatagaatta agtttacctc ttttcactct aaattgtcaa cgcaaaacgc acaaatatat
     6421 tttgacattt tgaatatatc tgatatactg aatatagcaa atatatttag tatatccact
     6481 atattcctta taaagtctga attcttatca atttggagta tattagatat atcgaatata
     6541 ttaagtatat gaagtatact aaatatattt agtataggaa attggaggga actatggaaa
     6601 acttacggac tgcatttaaa acatcagata aactaggaaa agttgtaatg cttacaggta
     6661 gaaaaggcgg cattggtaag actacagaca atgatttatt agcgattgtt tctagtcaac
     6721 tttttgaaaa agacgtgttg ctaattgatt atgaccaaca aagaaatact acttccaaca
     6781 ttggatcaac gtatcaaatt actagctttg atcgatccat gtctgcagca atcaaaaaag
     6841 gggactgggt ttcaggtatt actcaagtaa gtccacattt atatattatg gctggctctc
     6901 ctggatctga agaacttaat gaatacttgt ccgaaaaata tcctgatcgt cgtaagagat
     6961 cattggcctt cattaaacct ttagaagaat tgagaaaaaa tttcgattat atctttatcg
     7021 actgcccacc atcaacagat aatgtggttc gtgcattctt aactgctgca gattatatta
     7081 ttgcaatgca agaattaaaa cggtacgcaa tggaaggaac agaagatttt attaataaag
     7141 ttttagttcc gattgtcacg aattttgaag agtctcatct tcaaatcatt ggaattttgc
     7201 ctgttctttt ctcagtgcga cgttcctctc aacacgcaaa ctatcaaaaa acaattgata
     7261 agtatggtga atcaaacatt tttaaaagca ttgttaaagg ctcggatcgg ttagaaatgt
     7321 atggtgagaa tggtatccaa ttaaatgatt atgtagatcg aagatggtgg gctatttttg
     7381 cagacatctt tacagaatta gaagaaagaa ttgaatacta tgaacaacat ggtgatattg
     7441 aaggatttga ttatacacct aaatatgctg actcaatggc caatcatatc ctacctcttg
     7501 gaaaggagat taaactaaat ggcattatta ccaacgaaca aagataagaa gaaaattggt
     7561 gctgaagata tttcaattaa agatgatatt aatgtcgagt taggggaacg aaagtacact
     7621 gcgaaagaaa gaaaaccagt tcaagtagat cctcccgtac taaaaaccat tcgggatctt
     7681 agttatgcaa aagatattcc tatgtatgaa atagtcaaac ttgcaatgga cgcctatgtt
     7741 gatactttaa acgacgagga acggattata tataatagac gatcaaaaga ttaaaatata
     7801 gtaagtatac tagatatagt aaatatattt agtatactta ctatattttt agctttatta
     7861 atcctaatcc ttcaaaatat gtgtattgtt tgggggtata caacattcac aaattttatt
     7921 gtttctagtt tttactatac taaatatggt tctgttgcaa agttttaaat ctactatcaa
     7981 ataaggtaga ataatagaaa aagatagcag gaggaatgac gatgaatcat tttaaaggaa
     8041 agcaatttca gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta
     8101 gctatcgtga agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga
     8161 tttatcgttg ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata
     8221 aaaaatcctt ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc
     8281 attatttgta tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa
     8341 aacgggacac acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac
     8401 cgaaggttgt agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag
     8461 aatacggctt ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg
     8521 aacaagacca tcgtccagta aagagacgca ataaattcta tcgaagttta cgcactgcct
     8581 ctaccacgat taaaggcatg gaagccattc gaggattata taagaaaacc cgaaaagaag
     8641 gcactctctt cgggttttcg gtctgtactg aaatcaaggt attattggga atcccagctt
     8701 aaatcataga taccgtaagg gattttattc tttatttaaa actttgcaac agaaccattt
     8761 atagagtaca gaaaacattt tctgtactct attttgtctt cttttaaatt ttcaatagat
     8821 tatatctatt gtgaattttg tagagggtca gaaacttaat agtttttgac acataaacaa
     8881 aatcgcgata taatggggtt gagagtgttt cataaactta tgtcataaag ttaaggagat
     8941 ttcgcatgat tttagagaat aaattgggat taaccaatca agttgagttg gctaaggttg
     9001 aagaaaaact cagtaaacaa aaagcgaaag aattgtacga ttgtggaaaa ataaatgaaa
     9061 tcgaagttgg tacatttaaa ggtttatctg aaattcatga atttctattt tcggatattt
     9121 acgattttgc cggaaaaatc cgatcggtca atattgcaaa aggaaatttt cgatttgcac
     9181 cagttatgta tttagaacat tcgttacagc atattgatca gatgccacaa acttcttttg
     9241 aagagattat caagaaatat gtggagatga atatcgctca cccatttcga gaaggaaatg
     9301 gccgaagtac tagaatttgg ctggatctca ttttaaagga agaattgcaa aaagttgtag
     9361 attggaactt aatcgataaa gcagactatc tttctgcaat ggagcgaagt cccataaacg
     9421 atttggaaat acgctatcta atttccaatg ctctgactga taaaattgac gatcgcgagt
     9481 tatatatgaa aggaatcgat gtcagctatt tctatgaagg gtattctgag tatacgatag
     9541 atgatttgta aaagtaggtg gaccaaatga cgaaatatgg atatgcacgt gtctcaaccg
     9601 cttctcaaag tacaaaagaa caaattcaac agttgcttga gaacggcgta gagaagaaga
     9661 atattttttc agaaaaattt acgggtacaa aacgggatcg gccaaaattc aatcagctgt
     9721 tggctggtct gaagcctgga gatgaaatag ttgttacgaa gcttgatcga tttgctcgta
     9781 acacgagaga agcattagag atcattgaac ctttactcga tgataacgta acgatcaagg
     9841 ttttgaatct aggaacgatt gaaaatacgt ccatggggcg aatggtgaca cggacgttgt
     9901 tgagtgtagc agaaatggaa cgtgatatga ttgtggaacg tacccaagaa ggaaaaatgt
     9961 ttgcaagaaa gaataatcct aatttcagag aaggacgtcc gaaagcaaca atcacaccga
    10021 agaagaggca cgcatatgaa ctgcttactt caggtaaatc gtataaggaa gttgaagcta
    10081 taaccggttt cagtcgcagt acgcttttta gaataaagaa gaaaattgaa gaaagttaaa
    10141 aaagaagtct cagcgaacca gacaggtaaa tacttaatgc gttctagata tgagtctatt
    10201 agcatgttgt gaaatataat atttttttat ttacttgatt tctgctcaaa aaatgtgtat
    10261 ggtaactgta aaaggtaaag taattaaagg tatgctagcc acgcttacta tattaggata
    10321 ccatcatata tttataatga acgatttgtt cgtctaccaa acaacacata tcgttcagcg
    10381 taagtcacgt ttaggaacgt ggctttttta gtgttattat cgataaataa tctattgact
    10441 ctattgattt tatctccata tccctacggt gttgattgtc tttagatctt tcatttaaga
    10501 accaaataac tatcataaaa atcagcactg ctgctaaggt tctgttgcaa agttttaaat
    10561 aatttgattt gtcaaattaa gctagacaaa atatcttcat tcatgtaact cataaatact
    10621 tccaggggtg tttgataatc caaggacttt cgtggaatat tgtttctctt attcgccact
    10681 gaagagataa aagcttggtc gacttgatta aaatccatgt tttttggcag accatctttt
    10741 cgcagcaagc cattggaatt ttcattcagt gctcgttgcg aaggagtccc tggatcagcg
    10801 aaataaatag cgatgtcatg ttggttactt aaagccttcc aattagagaa ttctttccca
    10861 caatcaaagg taatggattt aaataaatgt ctgggaatcg atccaaacca gtggttcaga
    10921 gtattctcaa tatcgatcgc cttgcgcccc tcgggcttca aggtgatgat gattttcgat
    10981 agacgttcta ccaaagttat gacagcactc ttatgatgaa tgcctacaat ggtatcgcct
    11041 tcgatatggc cgaactcttc tttaaatgtc gggtaatcag tttctctttc aacgatattc
    11101 cgtttaaaag cctgttttcc tcggcgttct tgatgtccat ttggtttccg cttacccttc
    11161 atgggcaaag tcgattcatc gaacaccttt tccttgaact ggcgataaag ggttcttacg
    11221 gaacagctga ttggtttctc tttacgccca atgataacgt ctggcgtcca gccttgggca
    11281 attttttctt taatgtagga ctgttgtttc tttggcaaga cgatgtttcg tctgccacac
    11341 cgtttcttat tttctttata ctgttggtag tattcgaggg ccgtatggcc ttctttcaag
    11401 aaattaatga cgttataaat cggtgttcga gatcgtttca ggtactcagc aattttcaca
    11461 attttgatgt tttggtggta gtaagactct atcatcacca gttcatccgt ggtaagatgg
    11521 gtgtaggtca ttcgtgatca ctcctatgtt ttctttagtc ggaattacat tttgagtgta
    11581 gcacgaatgg cttatttatt tgtctagctt aattttacaa tcggcgtaaa gaataaaatc
    11641 ccttacggta tctatgattt aagctgggat tcccaataat accttgattt cagtacagac
    11701 cgaaaacccg aagagagtgc cttcttttcg ggttttctta tataatcctc gaatggcttc
    11761 catgccttta atcgtggtag aggcagtgcg taaacttcga tagaatttat tgcgtctctt
    11821 tactggacga tggtcttgtt caatcaaatt attcaggtat ttaatggtac gatgttctgt
    11881 cccttgataa aagccgtatt cttttagttt cttaaaggca cttgtaatag agggggcttt
    11941 atctgtgact acaaccttcg gttcatcaaa ctgcttcact aaccgcttaa gaaaagcata
    12001 ggctgcttgt gtgtcccgtt ttttacgtaa ccaaatatcc aaggttaaac catctgcatc
    12061 gatggctcga tacaaataat gccattttcc tttaattttg atgtacgttt catccatttt
    12121 ccatgaataa aaggattttt tatttttctt tttccaaatt tgatagagta gtttgccata
    12181 ttcttgcacc caacgataaa tcgtcgtatg agaaacgtta atgccacgat catataagat
    12241 ttcttgaact tcacgatagc taaggttata acgaagatag tagcccacgg ctacaataat
    12301 cacatcctgc tgaaattgct ttcctttaaa atgattcatc gtcattcctc ctgctatctt
    12361 tttctattat tctaccttat ttgatagtag atttaaaact ttgcaacaga acccttccac
    12421 ctaattcatt gagtacagaa gaatccatta agcaaggtgt gaagtatttc agtgaattat
    12481 tagccagtag cgaaaggctc agtgtagatt tagaatcggt tatccagtcc tacaattatg
    12541 gtggtggttt cttagggtat gtggctaatc gtggaaataa atataccttt gaactggctc
    12601 aaagtttctc aaaagagtat tcaggtggcg aaaaagtgtc ttaccccaat cccatagcca
    12661 tacctatcaa tgggggctgg cgatacaact atggcaatat gttttatgtg caactggtaa
    12721 cgcagtatct tgtcacaaca gagtttgatg atgatacggt acaagccatc atggacgaag
    12781 cactgaaata tgagggctgg cgatacgttt acggtggagc ttccccgact acttcttttg
    12841 attgtagcgg actgacacaa tggacgtatg gaaaagctgg aattaactta ccacgaaccg
    12901 cacaacagca atatgatgtg acccagcata tcccactatc ggaagcacaa gctggcgatt
    12961 tggttttctt tcattctacc tataacgctg gctcttatat tactcatgtt gggatatacc
    13021 ttggcaataa ccgtatgttt catgcaggcg acccaatcgg ttatgccgac ttaacaagcc
    13081 cctactggca acagcattta gtgggagcag gacgaatcaa acaatgagaa aggaagattt
    13141 aatgatgaaa tttagaaaaa atcagaataa agaaaaacag ataccaaagg aaaagaaacc
    13201 tcgtgtctat aaggtcaatc ctcataaaaa ggttgtgatt gccttgtggg tacttttagg
    13261 gcttagtttc agctttgcga tattcaagca ctttacagct atagatactc atactattca
    13321 cgaaacaact atcatagaaa aggaatacgt tgatactcat catgtagaaa attttgtaga
    13381 gaactttgcg aaagtctact attcatggga gcaatccgat aagtccattg ataatcgaat
    13441 ggaaagtcta aaaggctatc tgacagatga acttcaagct ctcaatgttg atacagtacg
    13501 caaagatatt cctgtatcgt cttctgtaag aggatttcag atatggacgg tagagccaac
    13561 tggcgacaat gagtttaatg taacctacag tgtagaccag ctcattacag agggagaaaa
    13621 tacaaagacc gtccactctg cttatatagt gagtgtctat gtagatggtt ctggaaatat
    13681 ggtactggtt aagaatccga ccattaccaa catacctaag aaatcaagtt ataaaccaaa
    13741 agccattgaa agtgagggga cggttgattc cattacaacc aatgaaatca atgagttttt
    13801 aacgacgttc ttcaagctct atcctacagc gacagccagt gaactttcct actatgtgaa
    13861 tgacgggata ttaaaaccaa tcggaaaaga gtacatcttt caagaactgg taaatcctat
    13921 tcacaatcgt aaggataatc aagtcacggt atcgctgaca gtggagtata tcgaccagca
    13981 gaccaaagca acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg
    14041 gaagattgta aaataacaaa tattggtaca tgattacaga tactttgtaa tcatgtactc
    14101 tttttgataa aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt
    14161 gactatttaa aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt
    14221 gatagcagga acaaataatt agatgtcctt ttttaggagg gcttagtttt ttgtacccag
    14281 tttaagaata cctttatcat gtgattctaa agtatccaga gaatatctgt atgctttgta
    14341 tacctatggt tatgcataaa aatcccagtg ataaaagtat ttatcactgg gatttttatg
    14401 cccttttggg tttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag
    14461 ctcatgttga tgcaggaaaa actaccttaa cagaaagctt attatataac agtggagcga
    14521 ttacagaatt aggaagcgtg gacaaaggta caacgaggac ggataatacg cttttagaac
    14581 gtcagagagg aattacaatt cagacaggaa taacctcttt tcagtgggaa aatacgaagg
    14641 tgaacatcat agacacgcca ggacatatgg atttcttagc agaagtatat cgttcattat
    14701 cagttttaga tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc
    14761 gtatattatt tcatgcactt aggaaaatgg ggattcccac aatctttttt atcaataaga
    14821 ttgaccaaaa tggaattgat ttatcaacgg tttatcagga tattaaagag aaactttcta
    14881 tggaaattat aatcaaacag aaagtagagc tgcaccctaa tatgtgtgtg atgagctgta
    14941 cggaacctga gcaatgggat gtggtaatag aaggaaatga tgacctttta gagaaatata
    15001 tgtccggtaa atcattagaa gcattagaac tcgaacaaga ggaaatcaga agatttcaga
    15061 attgctcctt gtaccctgtt tatcatggaa gcgcaaaaag caacataggg attgagcagc
    15121 ttatagaagt gataacgaat aaattttatt catcaacata cagaaagaag tctgaacttt
    15181 gcggaaatgt cttcaaaatt gaatattcgg aagaaagaca acgtcttgca tatgtacgcc
    15241 tttatggcgg aatcctgcat ttgcgggatt cggttagaat atcggaaaag gaaaaaataa
    15301 aaattacaga aatgtatact tcaataaatg gtgaattatg taaaattgat aaggcttatt
    15361 ccggggaaat tgttattttg caaaatgagt ttttgaagct aaatagtgtt cttggagata
    15421 caaagctatt gccacagaga gagagaattg aaaatccgct ccctctgctg caaacaactg
    15481 ttgaaccgag caaacctcaa caaagggaaa tgttacttga tgcactttta gaaatctccg
    15541 acagtgaccc gcttctacaa tattatgtgg attctacgac acatgaaatt atactttctt
    15601 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
    15661 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
    15721 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
    15781 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
    15841 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
    15901 atggttggaa tgtgacggat tgtaaaatct gttttaagta tggcttatac tatagccctg
    15961 ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa
    16021 aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat
    16081 atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat
    16141 tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc
    16201 gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt
    16261 accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata
    16321 aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg
    16381 tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag
    16441 gaattaatta tgaagtgaaa tgaatgtaac agggttcaat taaaagaggg aagcgtatca
    16501 ttaaccctat aaactacgtc tgccctcatt attggagggt gaaatgtgaa tacatcctat
    16561 tcacaatcga atttacgaca caaccaaatt ttaatttggc tttgcatttt atcttttttt
    16621 agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa tgattttaat
    16681 aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt ttccattgga
    16741 acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact cctatttgga
    16801 attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt cttttcctta
    16861 cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc actcgtaatg
    16921 gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg tcttattgga
    16981 tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat agcccattat
    17041 attcattggt cctatcttct actcattcct atgataacaa ttatcactgt tccgtttctt
    17101 atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa aggaattata
    17161 ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag catttctttt
    17221 cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa agtaacagat
    17281 ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt tctttgtggg
    17341 ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat gatgaaagat
    17401 gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg aacaatgagt
    17461 gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc tttatacgtg
    17521 ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt tcttttagaa
    17581 acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct ttcgttcacc
    17641 aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc tggtgctgga
    17701 atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc aattgtaggt
    17761 ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt tgatcagtca
    17821 acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat tagttggctg
    17881 gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa gggatcaact
    17941 ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc tttttgttcc
    18001 attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat cttttttatt
    18061 ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt tattcttgct
    18121 gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca agggtttaaa
    18181 ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc tgatttttaa
    18241 acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt tttttgtttc
    18301 ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc ggcactgccg
    18361 acagcctcgc agagcccacc tttacgaagt aaagtatagt gtgttatact ttacatggaa
    18421 gttgtaccga aatattgtgt tgtgaagtct atataatttt aaaattatga agtctatata
    18481 cgtgcgtaaa gatatttaaa gagattaaaa ataataaaaa taaaaattga attaaaaaga
    18541 gatatggttg attattggaa cgattgtttt aatgatttac atatattaca accagattgg
    18601 aaaactatcg aacgtacttc tgatagagca atggttttta tgttgttgaa tgatgaagaa
    18661 gaatggggaa aattagaaag acgtactaag aataaatata aaaaattaat taaagaaata
    18721 tctctaattg atttaactga tttaatgaaa tcgactttaa aagcgaacga aaaacaattg
    18781 caaaagcaga ttgatttttg gcaacgtgaa tttagatttt ggaagtaaag taactttgtt
    18841 ttatacaaaa tgttacttta gtataattaa cgtttaagag gtgacacaat atatgaaaaa
    18901 atcagaggat tattcctcct aaatacaaag agataagatg aaatttagga ggaaaattat
    18961 atatgacttt taatattatt aatttggaaa cttgggatag aaaagaatat tttaatcatt
    19021 atttcaatca acaaacaact tacagtgtta ctaaagaatt tgatatcact ttacttaaaa
    19081 gtatgataaa aaataaagga tatgaactgt atcctgcttt gatttataca attgtaaata
    19141 ttataaatca aaataaagta tttagaacag gaattaatag tgagggaaat ttgggttatt
    19201 gggaaaaatt aaacccttta tatacagtct ttaataaaga aactgaaaaa ttttctaaca
    19261 tttggacaga atcaaatgtt agttttaatt ctttttataa tagttataag agtgacttac
    19321 ttgaatataa agataaaaat gaaatgtttc ctaaaaaacc aatacctgaa aacacagttc
    19381 ctatttcgat gattccttgg attgatttta gttcatttaa tttaaatatt ggtaataata
    19441 gtagattcct attgccaatt attacaatag gtaaatttta tagtaagaat aataagatct
    19501 atttaccagt ctcattgcaa gttcatcatg cggtatgtga tggttaccat gtttcattat
    19561 ttatgagtga atttcaaaat atagttgata gtgtaaatga atggatttaa atctaaaata
    19621 actttcataa acttaagtaa ttatgatatt tataagttta tagcaactac catttttggt
    19681 tgctggtgtt ttgattttga atttggtttt gaacttatgg actgatttat tcagtccatt
    19741 ttttgtgctt gcacaaaaac tagcctcgca gagcacacgc attaatgact tatgaaacgt
    19801 agtaaataag tctagtgggt tatactttac atggaagttg tgccgaagtt aggagtgttt
    19861 acatgtcatt tgcagtggcg agaatgcaga aggtaaaatc aggaaattta gtcggggtag
    19921 gtaatcataa tcagagaaat acagacaatc attccaacaa agatattgat gttgaacggt
    19981 cgcatttaaa ttatgatttg gtcaatcgga cagaaaatta taaacgagat attgagcaat
    20041 ttattaacga caataaatca agtagtcgtg ctgtcagaaa agatgctgta ttaataaacg
    20101 aatggatcat aacttctgac aacccatttt ttaaagataa agacgataaa gaaattaaag
    20161 atttttttga tactgcgaaa tcatattttg cggacaaatt tggcgataat aatatccgct
    20221 acgctcaagt tcacttagac gaaacgacac cgcatatgca cttaggaatt gtgccgttta
    20281 atgacgaaca caagctatca gcaaagactg tttttaatcg tcaagcctta caagcggtac
    20341 aagacgaatt gccaaagtat ttaaatgagc gtggttttga gcttgaacgt ggcgaaaaag
    20401 ggtcagagcg taaaaattta acggtgcctg aatataaaaa ggcaaaggac gaattaaaag
    20461 agataactac cacgctggaa cagcgaaagt cagaagtttt agcgttatcg aacgataaag
    20521 caccaacaat caagaaagaa agtttagatt taaaagacga aacgaaaacg gtcaaagtac
    20581 caagtggcga aaccattggg attggtaagc tgcgacatga atttatgaaa aatgaagaac
    20641 gaaaaacggg aaatgtaatt atccctgaaa gtaaattaaa tgcagtgatt aaaagctatg
    20701 aagagctata taaagcaaat gaaaagctaa aaaattatgc ggaaactgat ttacctaaag
    20761 aaatcactag agttaaagag aaatatcgcg aattagcgaa agattacaat aggttggtac
    20821 gtaagagtaa tcagaatatt gaaacacttg aagaattaga gaaagagaat aagtcgctta
    20881 aaaacgaaat taagggcatt tacaaaggtt ttaaccagtt tatggaaaat acccttggtg
    20941 caactgttgg acaagctaaa aagctgatga ataaccttat gagcgaagtt aaagagtttg
    21001 taaaaggtgg agagtttgaa aaaattcata gaaacgaaac taaaacacga gatagggatg
    21061 aattaagtcg ttagaagctc atatttgcgt tctaagcgct tttttatcaa aactagggta
    21121 attctgcata ttgtttactt ttcgtcggca aatgcgtaaa aaacagcgaa ataaaagagg
    21181 tcaagggcga acgtagtgag tgtatttacc cttgatctct ttttttgagt tgtttagctt
    21241 ttgctaagac gacggaaaga aacgaatgca gttatcaagt tgcttttcag aggcaaggtc
    21301 aagcgctttg cgcttgttgg gtgtgggcga atagcccacg gttttattat ttggaaattt
    21361 agtgggagca gggggcgaaa aaacatggga atttttggag cgaaaaaaca tgggggcact
    21421 acgacacccc ccatgtgtcc attgtccatt gtccaaatag ataaaaagat taaatgtctt
    21481 gtaaatacct ttatatgaac gttttaagca cctatttcga ggtgaaaaaa agttttttgt
    21541 aaaatcttgt attcctgtcg tatgtataat ataattacag tgtaaaagag attttgaaga
    21601 gaattgaggt gcattttgtg gttgaagttg agaagaaaaa agtgacttta tctttacctg
    21661 ttgaatcgaa tgacaaacta gaaaaaatgg cacaaaaata tggaatgact aaatcaggtt
    21721 tggttacatt tttaattaat caagctgatg ataaggggac tatttttaaa taaaaaaagc
    21781 cctgctagga agatagcaag ggctcgcaag atttggtttt ctttgatttc gcaactcaag
    21841 tttatcaaat tttcccttgt tttcctagta gagtaactag gaaggtgtag aaaaatatgg
    21901 aaaatgaaaa tagaacagat tggacattgc ctagaaagaa tttaaatcca aaaactaaac
    21961 agccttataa gcgaggtcgt aactggtgga ttgttgttta tcctgaaagt ttgccagaga
    22021 attggaaaga gattatttca actgaacctg tggcaattag tccattacat gataaagatg
    22081 ttaatgcaga cggaacaaag aaaaagccgc attatcatat agtttttaat tacaagggca
    22141 ataaatcatt tgaacaaatg gacgaaatgg caagagtttt aagggcaccg attcctgaaa
    22201 gaataagtgg tttaactggt gctgttagat atttaacgca tatggataat cctgaaaagt
    22261 atcaatacga taatacagaa atacaagtgt ttggtggatt cgaccttgaa agttgtttag
    22321 cgttatctac tggtgataaa agacaagcgt taaaagaaat gcttggtttt atttcggata
    22381 acaatattat gcatttaaag gttctgttgc aaagttttaa ataaagaata aaatccctta
    22441 cggtatctat gatttaagct gggattccca ataatacctt gatttcagta cagaccgaaa
    22501 acccgaagag agtgccttct tttcgggttt tcttatataa tcctcgaatg gcttccatgc
    22561 ctttaatcgt ggtagaggca gtgcgtaaac ttcgatagaa tttattgcgt ctctttactg
    22621 gacgatggtc ttgttcaatc aaattattca ggtatttaat ggtacgatgt tctgtccctt
    22681 gataaaagcc gtattctttt agtttcttaa aggcacttgt aatagagggg gctttatctg
    22741 tgactacaac cttcggttca tcaaactgct tcactaaccg cttaagaaaa gcataggctg
    22801 cttgtgtgtc ccgtttttta cgtaaccaaa tatccaaggt taaaccatct gcatcgatgg
    22861 ctcgatacaa ataatgccat tttcctttaa ttttgatgta cgtttcatcc attttccatg
    22921 aataaaagga ttttttattt ttctttttcc aaatttgata gagtagtttg ccatattctt
    22981 gcacccaacg ataaatcgtc gtatgagaaa cgttaatgcc acgatcatat aagatttctt
    23041 gaacttcacg atagctaagg ttataacgaa gatagtagcc cacggctaca ataatcacat
    23101 cctgctgaaa ttgctttcct ttaaa
//
