LOCUS       AP031239                5272 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE6 plasmid pNUITMV6_6 DNA, complete
            sequence.
ACCESSION   AP031239
VERSION     AP031239.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639535
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 60x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE6"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV6_6"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(255..620)
                     /locus_tag="NUITMVRE6_31420"
     CDS             complement(255..620)
                     /locus_tag="NUITMVRE6_31420"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97594.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFMR
                     NASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLDYK
                     TFYPELLEFFQKLNSLE"
                     /name="hypothetical protein"
     gene            complement(629..790)
                     /locus_tag="NUITMVRE6_31430"
     CDS             complement(629..790)
                     /locus_tag="NUITMVRE6_31430"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97595.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLTE
                     IIELDSKE"
                     /name="hypothetical protein"
     gene            complement(780..929)
                     /locus_tag="NUITMVRE6_31440"
     CDS             complement(780..929)
                     /locus_tag="NUITMVRE6_31440"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97596.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGEQ
                     KNDG"
                     /name="hypothetical protein"
     gene            complement(943..1692)
                     /locus_tag="NUITMVRE6_31450"
     CDS             complement(943..1692)
                     /locus_tag="NUITMVRE6_31450"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97597.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVLD
                     YCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVERP
                     DGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGKYAL
                     IMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELSDKMS
                     VSFFVTTLKRGRKVVGYEITINDTSKE"
                     /name="hypothetical protein"
     gene            complement(2659..3156)
                     /locus_tag="NUITMVRE6_31460"
     CDS             complement(2659..3156)
                     /locus_tag="NUITMVRE6_31460"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97598.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRVV
                     FALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQAL
                     KIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYFELN
                     SQ"
                     /name="hypothetical protein"
     gene            complement(3184..3639)
                     /locus_tag="NUITMVRE6_31470"
     CDS             complement(3184..3639)
                     /locus_tag="NUITMVRE6_31470"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97599.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGYI
                     KVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQVQ
                     KFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
                     /name="hypothetical protein"
     gene            complement(3647..4633)
                     /locus_tag="NUITMVRE6_31480"
     CDS             complement(3647..4633)
                     /locus_tag="NUITMVRE6_31480"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97600.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLAQ
                     TQLEATRRIFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPNHE
                     IYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQPKE
                     KFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNKGKRI
                     GFKWVDDEGKDFTIGGRKLGSDFERPAIERTLEFSQQKQEKEVEKPLETKPKVTNDDFS
                     ISGLKNLANDIRQQEERKKKEELQRQKEIERQQRSRPFYHDRGGMER"
                     /name="hypothetical protein"
     gene            complement(4618..4974)
                     /locus_tag="NUITMVRE6_31490"
     CDS             complement(4618..4974)
                     /locus_tag="NUITMVRE6_31490"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97601.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLCK
                     SVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQQL
                     SQIEKGVIDLWEQL"
                     /name="hypothetical protein"
ORIGIN
        1 gaggtggcgt tgccccctac gaactccccc aatcacgcaa caaaaagtag cctgttcggc
       61 tatttttatt acgtgatttc gcctcagaaa aagtagtctg ttcgactatt tttctactgg
      121 ctcatgcggt caaacttgca cgcaagttga ccttattttt ttgtttgccc gctgttcgca
      181 ggcaaacaaa aaagcaagcc aaaaatcggc ttgctcgttc ttcaaaaatt gatcgcatgg
      241 actagctcgt tccgctattc caagctgttc aatttttgga agaactctag caactcagga
      301 taaaatgttt tgtaatccaa cttgtttccc cagttgtctt ccatccattt ttcgagttgc
      361 ttttgaaatt gcaccgcttc ggctttcagc tcgctaggca atttttcggc agaagtggcc
      421 accggctttg cagctgctgg cgcaggtgca gatccggcgg acgacttcaa tgcctttgac
      481 gcattccgca tgaaagtatt gtaggcggaa tagctcttga acccttgttt ttcaaccata
      541 tttgtaccga ttttgactga aagatcatgc cgagcttcag ctaatttttt ttccaaatct
      601 tcgatatcca tttttgccat gcgtaaactc actccttaga atctagttcg ataatttccg
      661 ttagaagaag atcaaattgg ttgatcgttt cttctgaaag tccaccggtg tattcaataa
      721 tagcgtcact atgcctttct aacagctttt caagctgttc aacgtcaatc gtaactgtct
      781 tagccgtcat ttttttgttc tcctttttcg tcataactat gaatcaaaaa gccgtcacgt
      841 tcgccttttg catagttcat tagttctttc atagcttgat ctagcgaaga atttttcttt
      901 gccatctttt tacgcagtct ttttttcaac taaatcactc ctctattctt tgctggtgtc
      961 attgatcgtg atttcatatc caacgacttt tcgcccacgc tttaaggttg tgacgaaaaa
     1021 tgaaactgac atcttatcag ataattcttc aatagcacga gtaattactt tttgaaaaaa
     1081 ttgacctgca ggaaggcgtt tgtctttacc aagaaaccaa gattcccatt cttccaaatc
     1141 acctgtaatc gttgtgtgtt tttcttttcc tctacggtag gattcccaga gttttaacat
     1201 tatgagagca tatttcccat tgatccgaga taattcacct aaacgaaagc tataaaactt
     1261 ttctttgaga tcaagaacgt atggagctga atcagcagaa aatttgaatc ttactaaccc
     1321 gtcagcataa aaatcaatcc aagaaaataa ttgcgtcatt ctgagacctt gagtaccatc
     1381 ggggcgctcg acacgaagat aaagcgcagt attttcatta agctttttga aagcttcagc
     1441 tattcgacgg taagaatctc cagaggtgtt caacccaaaa tgtttgataa tttctaatgc
     1501 ggttacctcg tattcgtcat ctagttttga atctttagtt acaaagctaa aacagtagtc
     1561 taaaactcgt tgctcaaacg atttaagccc tccaaatgcc ttagctaagt cgtttccttg
     1621 cactactaaa taatcttgac ggcttagtaa gtcttttaat gcaatatcag cttttttatt
     1681 tggttgtccc aaactgaaaa tcctcctttt gtactttttt taaaataata ccatttattc
     1741 agtttttttg caatatttga attttcaacg tggaaaatag tatttttgac gtggaaaata
     1801 gtatttttga cgtggaaaat agtattttca acgtggaaaa tagtatttat aggcgctaca
     1861 aacgctggta taaagccatt ccttttcccc taaagtattt taaagtattt aaaagtaatc
     1921 tataaagtat tctctttaag cgcgcgggtg agaaaaaaat ttaaattcaa aaaaaaaaaa
     1981 atcccgccca aaccagttgt ctattttttt ccaacaggtt cgcgcatgaa ataaactgct
     2041 tctcacatca tcgacaattt atccccttta cctcgttcaa tcttacagcc acaaggcata
     2101 aaggagtgat cgctacgctc tcacattttg cttacagcag tcgcctgcgc tccttcgtta
     2161 aggtcaaaac cgttgttcaa ggagcggttg ggataggcgt aaaagcttgt tgtagcaagt
     2221 ttttagactg atttagatta aatcgttagt cttgttatac agcgccttta caggcggtca
     2281 gggcaagccc taacgaccca aaagccccat ctggtagacc atcttttttt tgcagggagt
     2341 gaatgctata ttaatcttgg cactaaagcc aactaacgct tacacaggtt gggaggtgta
     2401 ttccgtatgt acgaaaaatt tctaacgttg gttgtcgcac cgctatttgt cgggctagtc
     2461 attgagctag tttctcattg gttggacgag aaagacgaca actaaggctt agtgccttcc
     2521 aactcacacg cctagtcgca gggcgaaaaa aaagccagac accgtcgcat ggtgtctggc
     2581 ttttgtgcat tccgcactcg aaaaaattct aacgttttcg catggttatt atagcatgga
     2641 cgttggcaaa tctcaatttc actgactatt cagctcaaaa taggattcta catcgtcaaa
     2701 tcgctttgtg tagcggtctt tcagctcttt atcgggcaaa gtagcgatca cgtcacgatt
     2761 tttttgtacc caatccgccg taataacttc gcttggatag tcttttgggt taaaggtttt
     2821 cagcttttct tgactggcaa ttttcaatgc ctgctccagt tggtccgaaa ttttatcaat
     2881 ttttttctgt tttgcttcgg ctttcgcttc tagttgctct attttgctga ttttagggcg
     2941 tacagacagc gcaacgtccg gacggtatga agaaatgccg accgacacaa aaagcgctag
     3001 aaacgcaaaa cagagggcaa aaacgacacg tttcagcttt ggttcctcaa atttttctaa
     3061 gatcgagcga aaaatgcaga ataaactata cgaaccgacc aaaataatca cgacaggaag
     3121 aatatgccac cccatttttt caaagaacag tttcatttgc gttttaagcc ccctctcgtt
     3181 cgattacagg agctttagga ctgaaacaat aagaatagca gtgccaccga taaaaagccc
     3241 cacagaggct aatacggaca ttaaattcgc tattcgattt tgtttcagct cttcggcagt
     3301 cgctcgatca tcgttggcta aaaatttttg gacttgtggg cgcacttgcc ggctcaattc
     3361 gtcagataca gatttgctga tattaccgta gctatcaagc ttttggtcga tttcagcggt
     3421 tcttttttga acgtaggtgg cgtagcggtt catttcagct ttcagctcgc tcattttggg
     3481 aatatcttcg ccacctcgca ccttgatata gccttgtttt acgctgttat caaggctttt
     3541 cagcgtttca atgacttctt gattttgttg cttgatttcc gctagttcct gcttcacttt
     3601 ttgaagctcc tgcttacttg caaaatcatc taacggcata tcgtcactac ctttccatgc
     3661 caccacgatc atgatagaac gggcgagaac gttgttgacg ctcgatttct ttttgtcgtt
     3721 gcagctcctc ttttttcttg cgttcttctt gctgacgaat atcattagca agatttttca
     3781 aaccgctgat agaaaagtca tcattggtga ctttaggctt ggtttcaagc ggtttttcga
     3841 cttctttttc ttgtttttgt tgagaaaatt caagtgttcg ttcgatcgca gggcgctcaa
     3901 aatcagaacc taattttcgt ccgccgattg tgaaatcttt gccctcatca tcgacccatt
     3961 tgaagccaat ccttttgcct ttattgtact cataaacctc gacagcatag gcttcttgaa
     4021 gatttttttt gaaagaatca aaatcggtac tcatatcttg tgcgcccagg atcatatgct
     4081 tcattttttc tcgattactc attttgccct cggcgtgcat ttcaaaatca acttgtttga
     4141 atttttcttt cggctgttca atgccaaaac tcaataaaac cttatcgttt aggtcttgta
     4201 ggtggtagcc gacctcggta tcagagtgaa attttttacc ggtttcagtg tcgatactgg
     4261 cgaaaacaat gtgattatga atatagccgg attctgaatc ggtatgagtg cccatgtaga
     4321 tttcatgatt gggatagctt tttgcgattt catcaacgag tttagcaccg atctcatgcg
     4381 ctttttgcac gtccgccgga ttgtttttgt caaacgaatt atcaaacgat tgaacccaat
     4441 gaaaggcttg aataccgcct gttttggcga aaatccgtct agtcgcttct agctgagttt
     4501 gagccaattt attctcagct gacagtttgt gcgtataaat gcggtcacga tcaagctttc
     4561 catgccgatt tacatagcca atcagcgcag aagccccacg tttaccgcct tttaaagcta
     4621 aagttgctcc cataagtcaa tcaccccctt ttcgatttgc gaaagttgct ggttcatcaa
     4681 ggctaaatct gaacttgaag gcatttgccc cttattcatg ttaataaagc gagccatctg
     4741 attgagattt ccgccatatt tgcgcaactc tttggcaatt tgtatcgccc cctcccgatc
     4801 aacgttcggc gctcgatact tcatgccaag tgccacgctc ttgcagagct gtggcacact
     4861 cataccagtt tgatccgcaa tggcggtcaa atactggtac tcgtcttcgc tcactcgaaa
     4921 tttaatttgc cgactatctt ttcgattttc ggcattcaat ttctgctcgc tcatctcatc
     4981 actcctcaaa taaaaatgct ccctattcgg tcgctttttt cttctcgtca ttcttcgtgg
     5041 tcgatattca aagctacaaa aacgctccct attcagtcgc cttttttccg caatgaaatc
     5101 tccctcggtt atgccactac aaaatgaaag ttccctactc ggtcacttcc ctttttccgc
     5161 tctataaccg aatgatttat taacctcatc atactcccta ttcggtcgca taacaaggtt
     5221 aataaatcat ataatacgca agcaccgcat ttggtaccca aatacatcgc tt
//
