LOCUS       AP031238                6149 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE6 plasmid pNUITMV6_5 DNA, complete
            sequence.
ACCESSION   AP031238
VERSION     AP031238.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639535
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 60x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE6"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV6_5"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(816..1205)
                     /gene="mobC"
                     /locus_tag="NUITMVRE6_31350"
     CDS             complement(816..1205)
                     /gene="mobC"
                     /locus_tag="NUITMVRE6_31350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002498952.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="BFG97587.1"
                     /translation="MGLMSEQNQYLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC"
     gene            complement(1646..1933)
                     /locus_tag="NUITMVRE6_31360"
     CDS             complement(1646..1933)
                     /locus_tag="NUITMVRE6_31360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97588.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            complement(1953..2177)
                     /locus_tag="NUITMVRE6_31370"
     CDS             complement(1953..2177)
                     /locus_tag="NUITMVRE6_31370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97589.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     gene            complement(2626..2796)
                     /locus_tag="NUITMVRE6_31380"
     CDS             complement(2626..2796)
                     /locus_tag="NUITMVRE6_31380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97590.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2812..3345)
                     /locus_tag="NUITMVRE6_31390"
     CDS             complement(2812..3345)
                     /locus_tag="NUITMVRE6_31390"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFG97591.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(3366..4310)
                     /locus_tag="NUITMVRE6_31400"
     CDS             complement(3366..4310)
                     /locus_tag="NUITMVRE6_31400"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFG97592.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            complement(5154..5843)
                     /locus_tag="NUITMVRE6_31410"
     CDS             complement(5154..5843)
                     /locus_tag="NUITMVRE6_31410"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97593.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             1..6149
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3367..4310)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..834)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.604"
                     /coverage="91.0"
                     /evalue="3.99e-180"
ORIGIN
        1 ttgttcagtc gtaagagttc gttgttctct tcgtacttct tttgcagttt cgcgaatgct
       61 agctcgagct cttcgtagct cgttggttcg ttgttgattg taggctcgtt ctcgtctgtc
      121 cagctcatgt aaaatcgtct ccttttcgta atcctttcct agtttgttgg atcgtacttt
      181 tttctttttg tggatcaatt cgtaagtaaa atttttccca cgattcaagc aatgaatgcc
      241 gaatgtttta agagaatcac aaaattggtt atacgttcga gtttttttgt ttttcattgt
      301 ttgatcaatc gcaaaccgaa tttcgtcttt ccaaacaggt tcattttttt cttttaattt
      361 gatttcagac atggttcgtt tttcgggttt cttccctcgt tcagtgattg ttaagtcatg
      421 ttcaagacaa atctcgtcat ttaggtcata acatttgttt aaaaatgctt gaccatgggc
      481 ttgaaatttg ttcccagttt ccagattgac ggagttaatg acaatgtggt tatgaatatg
      541 gtctttgtct gtatgtgtgt aaatcgcgat ttggtggtca ggggctattt ttttcgcata
      601 ctctgcccct agttgattgg ctttttcagc ggtaatttct cctggtcgaa aagactggat
      661 caataagtgt gcttgtacac gatcgtcttt tccatacagc atgcgaattt gtttcatttc
      721 gcttttggca taatcaatgt cacagtttat ccctgttttg acggttgctc gtggttcggc
      781 gtaattgatc gctcggctac aagaagtggt tcggcttact ttaactgttg ccatatcttt
      841 tgtagctcct tttgcatgat ttttaggttt tcggataagc gttgggcgtc gccaggatca
      901 acgtcgtgtt ggtgtgtgtt gcaccacttg gcaatttgat tgatattccc gccagctttg
      961 gctaaatcgc gcgcgattgc ctgagcaatg tctggcgcca ctttcggtgt caccagacgc
     1021 gcgttttgtg ccttggcttt cacgaaagcc ggcacactca tttgcaaagc ttcagctgac
     1081 tgcttcagct ttgcaaattc ggattcgctc actcgaaaac tgatttgttt tggctcggat
     1141 cgataggtat attttttaga atgatcgcta gccaaatatt gattttgttc gctcataagt
     1201 cccacccttt cagtacgtcc tacggacgta tcgttcgcaa taccattgta cctaaaaagg
     1261 tacaataagt aaagctcact aaagctcact aaagggcttg gcaagctcta tatttgctag
     1321 ccaaatacct acgtatttgg tgcaaataac cgcttgtcaa aggggcgcaa gctccccctt
     1381 gaatccccct cccccccact ctccacaata caaaaaagac tagcaaatgc tagccacttt
     1441 ttgtaaagtt tcgcttgttt caccttcggt gaaattgttg caaatcaata attaggtaga
     1501 gctacatttt tttcgttgtt caaagattct tatgccattt actattaata aatctataaa
     1561 tatgataatt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagt
     1621 cccattttca ttttattcca aagaattaac tccaaatacc aatagaagcc catcctctat
     1681 atacttgaga ttccttacta acaatttctt gtaattctat taaagattta ggtaatccaa
     1741 atttatgttt taaaatatat cttccaagaa aactacttaa tttcatacta acttcaccaa
     1801 cactttttcc ctcactgatt tctttagaat aaagaaagag cttttctttt aagtcaggat
     1861 agtcatctat agttgcttca ttgtatactt tactaattgc atttaaaagt ttttcttctt
     1921 tagtaaaatc cattataata acctccctac cactaccttc taggtgccca tggaccatga
     1981 ttaacccaac cattaacaat aatttttcca atttctcctt tagcttgatt ccaatctacc
     2041 caacattttt ctttgttaca ataaagacca tttccatagt aagtagctgc atcaactttt
     2101 attcctgtac cgatgccagc tagacaagtt cctaatattc ctagaataac acaatgtttt
     2161 aatactttct ttttcatgat tgtttttcct tttcgttttt ttaaaattgc ttgttatatt
     2221 aaataatctt agtaatttta ttatataaca ttttattata taacaatgat taacgtcata
     2281 aaaagtagtt ttgaattcat aatcgtttaa ataaaattat aagatcagtt atttaaacag
     2341 ctagtagaca taatgtcgac tatgccaagt agcccttgta tcaaccgata cagaggctca
     2401 ctttgtgatt ttcgattggt ctaggtcaaa agctcttttt aagcttgatt ttctgcggtt
     2461 gcttctttcc gtttctttgt gaatttttac cagtcatttt ttactgggtt gttattttta
     2521 ataaaatatg ataaattttt ggtagcgctt cataagcgcg ttgctttttc aagaataaac
     2581 actcattatt ttttaactgg ctgctcgaaa gagcagtctt ttttattatt tatctgtttc
     2641 ttttacaaac aattcttcgt taggactgtt gtaataattt ggattcaatt tcaattcacc
     2701 atgtttattc cctttattga tcactgacaa taacatcgga ctgaatccta gaattcctag
     2761 taaaatagaa ccgaatagaa gattttttct tgtcatgaat gttccctcca gttatttcca
     2821 aagttgccac catttttttt cagtatcaat ttgtttgtct attgtttctg ttgcttcaga
     2881 aagagtcgtt gagtcattct ctggagcttc ttctgtataa gtcaactgta gtttttcttg
     2941 cagtttttcg atttgtttgt tggcttgtag ggtgagttgt tgttgctggt cgagtagttt
     3001 ttgtatatga gaattatttt gctctctgag ggcaagttgt tctcgtaaaa attcattttc
     3061 ttcacgcaac tgatctagca acttgtttgt aaccacgttt ttgcttggta tattaacatt
     3121 tttatcgttg tcttgttcgt actcgtgcaa cctatctagc aacatttggt tgcctaagtt
     3181 gcgagcaagt tgcaatactc cagaacgcaa tacaacctta taaccacgct catttttagc
     3241 tatcatggaa gaatctaatt tttggatatg tttcgatatt gcttgcctac tcatcttaaa
     3301 aagttctgac aattccttta ttgtaaaggt ttcttgtggt tgcatactgg ttgcctccta
     3361 ggttgctact ggaataaatt atctaaatca gataataaac tttttttctc tttttccgaa
     3421 agtgcggact ctgtttgttt ttcaagagct tttttctctt tttctagttg tttgatttgt
     3481 tggaggagtt caagttctcg tttcttctgt ttgatttttt cgttaattgt tgtagcgggt
     3541 tcttcgtatt ctttttgttt atcagctaaa gccaatgctt tattttggtc ttctttggtt
     3601 ggtgcatgtt tgttaaagtc gtctgctttt ttgatctcag gtttgaacgt aaattgatag
     3661 ccaataacag gttttccccg tccttttccg tatttctttt tgattgttag tcctttgaag
     3721 attgcagtta gttcttgacg tataggtttt ataactcgtt tatctatatc aggttgctta
     3781 taacttttag gaatatctaa taattcaaga aagtcttctt tggagaaata tgcgaaacct
     3841 ttggttctgt attgctttaa cagacgaaac atagtctttg catagccact ttgtagctcg
     3901 ttgaattgtt gtaaagaata gcgtacccac tcgtctaaat tatttaacaa aggcagtgct
     3961 ttttcgtaaa ttcgtatatc tacgtaagga ttttctacgt ccccgttgat tttaaattct
     4021 gtaaacatta caaacatatc gtaagaaagc ccagactcac ttctacgacc aaagcgtaaa
     4081 gataagattt tttcataagt tgcttttaaa tcttctacaa atcgtacatt tgcagttgct
     4141 ttgtagttac ttaaatcttt tagttggtca aacgtaaaac gaactgtctt atcccctttt
     4201 tcacgcattc tagacacgat ggaaaaaaac aaattcattt caaccggtgt aaattttctt
     4261 aaaggaattg tatttaattc atggtggtat tttacaatct cattagccat ataatcacct
     4321 caatccaatt atgaatgaca ttttttaaat gtcaagacat tcatatttga cgttcaaaca
     4381 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
     4441 taaacagaca aaatgtcctt cataacattc tgtatccatt ggtagagtaa ggacacagct
     4501 tacggtaaag aggtttaaaa agaggtttaa aaagagatat aaaaagaggg gggctaattt
     4561 tcccgcctaa aaaccacttc ttttttcttt aaacaggttc gcgcattttt caaaactgcc
     4621 aatccaaaac cttgcttcct acggtcgcca aaaaggtgtg atcgctacgc tctcacattg
     4681 tactgcgcag tcgcctaaag gctcctttgc taaaaacatg ttggtaaaga ggcctttgag
     4741 cggttctgaa tgcttatcta agccgtttta agacagtctt ggataaaaat ccttatctag
     4801 ctttaaagcg ccttacaggc ggtcagggca agccctaaca accctaaaat aagcgatact
     4861 taaaaacacc acacaagttt aattttttaa gatataataa cttgtcgcct atccgacagt
     4921 cttgcgactg gaaggaggtg tatctaatgt ttgaattgtt catttgcccc cttcttatag
     4981 aagtttgtgt ctctttattc agttattggt tgaataaccg aaacaagaag taaaatcaat
     5041 aaaggcgaca tcaacctgaa aaaaagcccc agagaatccg acctctctgg ggctttcgtg
     5101 tatctaatgt ttgatcattt gcatgtttag tttaacatga ttgccgatca aattcaattt
     5161 tgctgttgct gatctaacca ttccttgaca ctttttagat aatttaatgc cgtttctttt
     5221 ttcgttggat tttgtatgtt cgttgcttgc tgaagcagtg ctttataacg ttcgtaggta
     5281 actgattgtt gccgttcttt tcgttcctgt tcactagttg aaaaagggat tgtatcactg
     5341 aataaattat aacaagcttc ttctgtctgc gtatctttaa aatattgcac gtcttttttg
     5401 gttcgctcat agttgtcttg aacctctttt tgctgttttt ctggcaagtt ttgataaaga
     5461 cgagtgagtt tttccatgtt tttatccgtc aaacgttttt cttgattcac aaaaatatcg
     5521 ttcatttcct cataacaagt ggtaaactct tccatttttt gtgttaacac ttgctccttt
     5581 ttctttttat cacatgctgc ttcatacttt tgagtgatat cccctaataa aatacttctt
     5641 gaaatacgtt tatttccctc taaaacgccg aataaaccac ttaaaacgat tgataatgta
     5701 aatataaccc ctggcaataa aaacctctta aatcgaattt tagagccttt taatgaatat
     5761 cttgtttgat aagccttata gctaagccaa ataattccta aaatactggc aaaaatgagt
     5821 agatagggta aatagaatgc catttctatt tattctcccc ataccagcca aaaggctaac
     5881 agtgtcatga ttaagcaaca taagggggca agaatatcca ttgatcgctg cataattact
     5941 tttttctgtt ctttatttcg ctggtttaat agcgcatggt ggaatttttt gacttgtgaa
     6001 tcaattgtag tttgtattct ttctttggta taaggtgcaa gaaaagcacg ttgtttcttt
     6061 atttcatttt ctatgtcttg taacgtttta gaacgataat tcttgttggt agtctgctcc
     6121 tctttcaacg tgttggtcat ggtttcttc
//
