LOCUS       AP031237                7056 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE6 plasmid pNUITMV6_4 DNA, complete
            sequence.
ACCESSION   AP031237
VERSION     AP031237.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639535
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 60x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE6"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV6_4"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(741..938)
                     /locus_tag="NUITMVRE6_31250"
     CDS             complement(741..938)
                     /locus_tag="NUITMVRE6_31250"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97577.1"
                     /translation="MISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQLYK
                     SGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(956..1411)
                     /locus_tag="NUITMVRE6_31260"
     CDS             complement(956..1411)
                     /locus_tag="NUITMVRE6_31260"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97578.1"
                     /translation="MGDFCLILLNENNATENVVIEYTEDGMVVTKYEDGYSAEDFANSS
                     DIMTRAAVARWGKKEYTNIAVTTGVAANAINAAFWAGGKASTSMFGIPGWAIGALLTGA
                     SWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGYDAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            1549..1953
                     /gene="tnpA_11"
                     /locus_tag="NUITMVRE6_31270"
     CDS             1549..1953
                     /gene="tnpA_11"
                     /locus_tag="NUITMVRE6_31270"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287522.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="BFG97579.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA_11"
     gene            1970..2506
                     /locus_tag="NUITMVRE6_31280"
     CDS             1970..2506
                     /locus_tag="NUITMVRE6_31280"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 2500"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97580.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPN"
                     /name="hypothetical protein"
     gene            2535..3116
                     /locus_tag="NUITMVRE6_31290"
     CDS             2535..3116
                     /locus_tag="NUITMVRE6_31290"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 2500"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97581.1"
                     /translation="MDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTN
                     LFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKL
                     AKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTC
                     PVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="hypothetical protein"
     gene            complement(3253..3378)
                     /locus_tag="NUITMVRE6_31300"
     CDS             complement(3253..3378)
                     /locus_tag="NUITMVRE6_31300"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97582.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSVNYSPLS"
                     /name="hypothetical protein"
     gene            complement(4046..4462)
                     /locus_tag="NUITMVRE6_31310"
     CDS             complement(4046..4462)
                     /locus_tag="NUITMVRE6_31310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390085.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97583.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(4464..4745)
                     /locus_tag="NUITMVRE6_31320"
     CDS             complement(4464..4745)
                     /locus_tag="NUITMVRE6_31320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390111.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG97584.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(5121..5645)
                     /locus_tag="NUITMVRE6_31330"
     CDS             complement(5121..5645)
                     /locus_tag="NUITMVRE6_31330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFG97585.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(5662..6630)
                     /locus_tag="NUITMVRE6_31340"
     CDS             complement(5662..6630)
                     /locus_tag="NUITMVRE6_31340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFG97586.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     Typing          complement(5667..6630)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cataaggaac tctttgagaa gctatgagcc ttcgtttgag acgttttaag acagtcttag
       61 tataaatccc ttatcttgtt cttaaagcgc cttacaggcg gtcagggcaa gccctaacaa
      121 ccctaaagtt gtctgaatgc cctctaaaat tcgatttaag cactcttaaa cctactctta
      181 tatatttata tacttagacg tttcaaacgt cttaaaacgc aaattagagc ttgataaaga
      241 tatttgctcg tgtgccgact tttttaaaag tctaggcaca cgccatgaca aagctgaaat
      301 aaactgcttc agctttgtca tggcggattc gctcacttga aaaagtgttc atttcgctcg
      361 attattagca tatatttttt aacttttttt gctagccaaa ttttgcatta gttcgctcat
      421 aaatcccacc ctttcagtac gtcctacgga cgtatcgttc gtaatgtgaa agtaccttta
      481 aaggtacttt ctacaaaact cactaaagtg tttggcaagc tctatatttg ctagccaaat
      541 acctccgtat ttggtgcaaa taaccgcttg tcaaaggagc gcaagctccc ccttgaatcc
      601 ccctcaaaat ccacgctcca caatacaaaa aaaggctagc atttgctagc cactttttgt
      661 aaagtttcgc ttatttcacc gaaggtgaaa ttgttgcttt gtaatattca aacaacccta
      721 ttcttctaaa atgtatcgat ttattttttt ttagacttta ttatgtctaa gacaactaag
      781 attaacacta aaaataaacc agatttgtat aattgccctc cgcttagcat attaacaatt
      841 atagcaacag ctatagcaaa tataaaaata cgaatagtag ctatttgcgg attcttttta
      901 gtaaatgttt ttaatgattt taatgattta gatatcaaaa acatcactcc ttaacttata
      961 acgttgaata tctagtagct acaactcttc cagctgcatc atatcccctt tggtaataaa
     1021 atccaatcca gccattacca tttttgtccc attcttttgc aactgcattc cctggagaag
     1081 cacctttttt agtccaactt gcacctgtta acaaagcacc aattgcccaa cctgggatac
     1141 caaacataga agtgctagct ttccctcctg cccaaaaggc agcattaata gcattagcag
     1201 ctactccagt ggttacagca atatttgtat attccttttt gccccatctt gcaactgcag
     1261 ctcttgtcat gatatctgaa gaatttgcaa aatcttccgc actataacca tcctcatatt
     1321 tagttacgac cataccatct tcagtgtact caataactac attttcagta gcattgtttt
     1381 cgtttaacag aattaggcag aagtcgccca tcctctatag gtgggtgatg aatgccgttc
     1441 ggtatgaggg ttaccgttgg taactcgaag accgacaact aaggcgtatt gcctttttat
     1501 tttgttccat gttacaatca gtctatacaa gaaatgagtt gatataacat ggaattagat
     1561 acaaataatc attcagtatt tcttctttat taccaccttg ttttagtaac gaaatatcgt
     1621 agacaagtga ttgatgatga aatatctgat tatgccaaaa ctacttttga aagaatttca
     1681 gaatcgtatc acattacttt agtggagtgg aatcatgata aagaccacgt tcatattatg
     1741 ttcaaagctc aacctaaaac agaattgaca aaattcatca atgcctataa aagtgccagc
     1801 tcaagattga taaaacgtga ctttccaaga gtcaaacaat ttctttggaa agaaatgttc
     1861 tggtctaaaa gcttttgtct tctgacaact ggtggcgcac caattgatgt gattaagaag
     1921 tatatccaaa atcaaggaaa caatcataaa tagaaagtag gtgaacttta tggaacaact
     1981 aaaagcatac aaattcagaa tctatccaac agaggaacaa gaaatcttct ttgccaaatc
     2041 ttttggttgt gtccgtaagg tctacaatct aatgcttgat gaccgaaaaa aggcgtatga
     2101 agaagttaaa aatgattctt ctaaaaaaat gacttttccg acaccagcta aatacaagaa
     2161 agaatttccg tttttgaaag aaattgatag ccttgcttta gccaatgccc aacttaattt
     2221 agataaagca tacaagaatt tttttcggga taaatccgtt ggatttccac gtttcaaaag
     2281 taagaaaaat cctgttcaga gttatacaac gaataatcaa aatggtacaa ttgctttgat
     2341 tgatagtaaa ttcatcaaag ttcctaaatt gaagtcatta gtaagaatta agcttcatag
     2401 acaaccaaaa gggattatca aatctgctac aatatcacgt cattctagcg gtaaatacta
     2461 tatttctttg ttgtgtaaag aagaaattat cgaattacct aactaattcc acaattggta
     2521 ttgacttagg tattatggac tttgcgattc tttctgatgg acaaaaaatt gataataata
     2581 aattcacgtc caaaatggaa aagaaactaa agcgtgaaca acgcaaattg tctaggcgtg
     2641 ctttattggc taaaaataaa ggtaccaatc tatttgaagc taaaaattat caaaaacaaa
     2701 aacgcaaagt agctagattg catgaaaaag taatgaatca acgtactgat tttctgaata
     2761 agttgagtac agaaatgatc aaaaatcacg atattatctg tattgaagac ttaaacacaa
     2821 aaggtatgtt gcgaaatcat aaattagcta aaagtatttc tgatgtttct tggtcaaagt
     2881 ttgtgactaa attacaatac aaggctgact ggtatggacg aaaaatcatc aaagtagata
     2941 aatggtttcc gtctagtcag atttgttcag agtgcggaca taaagatggc aagaaatctc
     3001 ttgaaattcg agaatggact tgtcctgttt gtcatactca tcatgatcgt gatatcaatg
     3061 ctagtatcaa tattctgacc gaaggtctaa gactacattc tatgggattg gcttagatcc
     3121 aatcatcaag aaccgtagga actacgggga tagcttggta aataagagac accgctgtta
     3181 gtaaagaaat acgctatcaa gtatgctcta ttcccaagaa gctcccactt caagcgttaa
     3241 gaaccgtcag gtttagctaa gtggtgagta gttcactgat accacttggg aattttctga
     3301 agcataaact atagtagttg acggtaaaac aactgcccca atagttaaca aagctacccc
     3361 taaagtaaat tttttcatag aatttcctcc tgatacagaa aaatatatag aaaacgtttt
     3421 ctttgtgaaa atcataaaac aaaaggtata ggcttgtcaa tattttattt agtaaagata
     3481 atttatattt ttgataatgt ggtatagtta gaatgcatta ggaaaatatg ttatattaaa
     3541 ttcttgttga cataatgtcg actatgccaa gtagcccctc taccaatgga tagagacgct
     3601 cactttgtga tttttaattg gtctagctag ttactagctt ctaatgcttg attttacgcg
     3661 atttctcttt tttagttctt tgtgcatttt tatctgtcat tttttaaaga cttgttattt
     3721 ttgagaggat ttgatatgct ttttttgtct ttctctagtt cttcactttg taatattttt
     3781 atgttgatga ataggataat ctcattttgg gctgctcgtg agagcagtct ttttttgttt
     3841 cagggtacgt cctattcttt ggatttccgc gctattaaat aaccgatact tggggattgg
     3901 ggtagcgcct caaaatacaa aaagaagaag ttgcataagc ggatttttct tttttttcgc
     3961 aagttggtac caacttgcaa atcttgctgg aagactgatc cctaaaaaga aatcgaggaa
     4021 tttttttagg gatcggtttc tttttttatc tttttagaaa gtttgttggg tcaaaatttc
     4081 cagaagtaaa ggtttgattg gtttcttttt gacgattttt gggttgattt cgtcgggaag
     4141 gattgagttg attttcaatt ttttctttca cttcttgata cactttagaa tttgtaccga
     4201 taagaacacc agtatctttt agatataaat cagcaaattc tgccatgatt tctgtcatga
     4261 tagaaatttc ttttcccatg gcattgagtt ttgcttggtt actggttgtg gttgtattgg
     4321 tatcaggttc ataactcata cataaaaaac gaatggcttc tgaataattg ttgacgtctg
     4381 gatttttttc gatgatggaa tctaaaacgt caacgtgact gggataaagg gttacgtgtt
     4441 tttgaacaga agtttttctc atgttatatt cctccgataa gtgtttcaat gagttggttg
     4501 tttgtgtgaa catagtcatt cagtatgttc gtattttctg tttgaatggc aataaatttt
     4561 tcgagtaata aatcgaattg atcttttttg taattcataa gttgacggtc aaaatcactt
     4621 tcgattagaa cttttagata tttatttcga ctccattttt tgcccgtttt ggttgtgagt
     4681 tcctgacaac gtttatctat tcttgcaata attttgggat tgatatttcg aactaaaatg
     4741 tccatggaaa ttttcctttc gtatcagcgt ataatttatg tataccatac aaatatagta
     4801 tttgtatagc tatttttcgc caaaacacta tacatagcct agccacaatg gcaaaaaagt
     4861 gatagcaaat ttttttctga gaaacgtgat aggcacagcc tatctagcac ctttcgtaag
     4921 aaagtgatac cacgtttcct tatgctcttt caaaattcag aaaaatcagc tgtaaaacga
     4981 gtgcagtccg agtaagattc aacgaaaaaa cggggattgg ggcgccacca aaatatcgca
     5041 acatgggtcc atgttgctct gctcgcaaaa gcaaaaagaa gaataaaacc agcaattttt
     5101 attaatttaa attgctggtt ttattgccag aattgccacc attttttctc cgcctttgat
     5161 tgtttttcta tcgattctgt ttcttcagaa agagtcgttg atttattttc cgaagattct
     5221 tccgtataag tcaactgtag ttgttcctgc agtttctcga tttgttggtt ggcttgcagg
     5281 gtgagttgtt gttggtggtc gagtaatttt tgcatttgtt ttatttgctc gtctttttct
     5341 tgtagttgca tatctttttg atttaactgt tgttctagga gagaaaggac ttctaattcg
     5401 ggaacttggt tggcgttttt gtggttttcg gttttcgttt tttcgccaac tagttgttga
     5461 tttgagtttt cgttttcgtt aaaaaacatt gattttatag ccttttgtcc ggtttcatta
     5521 atcacaaact tgtttccgat tttcgaaaac caaagttttc gattttcgtc agaaactttt
     5581 ttgttaattg ctgttttact aactcctaat tcttcagcaa gttctttgat tgttttttct
     5641 gtcataaaat ctaaaaaccc ctcaatcttt gaataagttt tgaagattgt ctaataagtc
     5701 tttttttagt tctgaaggaa tatcggaatt tatctctgat ttttgtgcat tattttcttt
     5761 attttgggta ttttgttctc tttctaactt ttctaactgt tgtaaaagtt ccaattttcg
     5821 cttttgttct ttgatttttt cttgtaatga agtaggtgct ttttcagatt gaacttgttg
     5881 gttagctagt tctaatgctt tgttttgttc ttcttttgtt ggggcatgtt tgttaaagtc
     5941 gtctgatttt tttatctcag gtttaaatgt aaattgatag ccaatgacag gctttccccg
     6001 tccttttccg tatttctttt tgattgttaa acctttaaag attgcggtta gttcttgtcg
     6061 tattggattg aacacacgtt gatcaatcgt accttggttg taacttttag gaatatcaag
     6121 taattcatga aagtcttctt ttgaaaaata agcgaaacct ttggttctgt attgttttag
     6181 taatcgaaac attgtttttg aataactgct ttctaattca ttgaattgtt ggagagaata
     6241 gcgcacccat tcttcaagat tattaagtaa gggtaaggct ttttcatgaa cttgtatttc
     6301 agcaaaagga atatcagatt ttccgtcgat tttaaattga ttaaataaaa caaaacgttc
     6361 acgttgcaaa ccgtcagcac ttcttctacc aaaacgtaaa tccattaatt tgtcatacgt
     6421 tgtttctaaa tcatctatga atcgaacatt tgcagttgct ttataattgc ttaaatcttt
     6481 taattgctcg aatgtaaatt gtactttttt atcacctaca tcacgcattc ttgatacgat
     6541 agaaaagaac aaattcattt cgactggtgt aaattttcgc aacggaattg tatttaattc
     6601 atgatgatac tttacgattt cattagacat atttaaaata gctcctttct tactcgcaca
     6661 agagatacac gttatgaaat acaaaatgcc tgtttatcta catcttatat taacatcgtc
     6721 gatgtactaa ttcaatgtac aaacagacaa aacgatgtag ataaacagac aaaacgatgt
     6781 agataaacag acaaaacgat gtagataaac agacaaaacg atgtagataa aagcgtcatt
     6841 cccttgggag agtaaggata tagccgacgg taaagaggtt taaaaagagg tttaaaaaga
     6901 gattataaaa gagggggaca aaatttcccg cctaaaaaac cacttctttt ttctccaaac
     6961 agattcgcgc atttttctca actgggaatc ttactcccta cagtcgctta aaaaggtgtg
     7021 atcgctctgc tctcacatta tactgcgcag tcgctt
//
