LOCUS       AP031228                6163 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE3 plasmid pNUITMV13_6 DNA, complete
            sequence.
ACCESSION   AP031228
VERSION     AP031228.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639532
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 76x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE3"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV13_6"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(352..522)
                     /locus_tag="NUITMVRE3_31430"
     CDS             complement(352..522)
                     /locus_tag="NUITMVRE3_31430"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94440.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(538..1071)
                     /locus_tag="NUITMVRE3_31440"
     CDS             complement(538..1071)
                     /locus_tag="NUITMVRE3_31440"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFG94441.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(1092..2036)
                     /locus_tag="NUITMVRE3_31450"
     CDS             complement(1092..2036)
                     /locus_tag="NUITMVRE3_31450"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFG94442.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            complement(2880..3569)
                     /locus_tag="NUITMVRE3_31460"
     CDS             complement(2880..3569)
                     /locus_tag="NUITMVRE3_31460"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94443.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3576..3866)
                     /locus_tag="NUITMVRE3_31470"
     CDS             complement(3576..3866)
                     /locus_tag="NUITMVRE3_31470"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94444.1"
                     /translation="MTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTT
                     IDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(3814..4728)
                     /locus_tag="NUITMVRE3_31480"
     CDS             complement(3814..4728)
                     /locus_tag="NUITMVRE3_31480"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="BFG94445.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4710..5099)
                     /gene="mobC"
                     /locus_tag="NUITMVRE3_31490"
     CDS             complement(4710..5099)
                     /gene="mobC"
                     /locus_tag="NUITMVRE3_31490"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002498952.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="BFG94446.1"
                     /translation="MGLMSEQNQYLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC"
     gene            complement(5540..5827)
                     /locus_tag="NUITMVRE3_31500"
     CDS             complement(5540..5827)
                     /locus_tag="NUITMVRE3_31500"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94447.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            complement(5847..6071)
                     /locus_tag="NUITMVRE3_31510"
     CDS             complement(5847..6071)
                     /locus_tag="NUITMVRE3_31510"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94448.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     BGC             847..6163
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1093..2036)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3818..4728)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtcataaaaa gtagttttga attcataatc gtttaaataa aattataaga tcagttattt
       61 aaacagctag tagacataat gtcgactatg ccaagtagcc cttgtatcaa ccgatacaga
      121 ggctcacttt gtgattttcg attggtctag gtcaaaagct ctttttaagc ttgattttct
      181 gcggttgctt ctttccgttt ctttgtgaat ttttaccagt cattttttac tgggttgtta
      241 tttttaataa aatatgataa atttttggta gcgcttcata agcgcgttgc tttttcaaga
      301 ataaacactc attatttttt aactggctgc tcgaaagagc agtctttttt attatttatc
      361 tgtttctttt acaaacaatt cttcgttagg actgttgtaa taatttggat tcaatttcaa
      421 ttcaccatgt ttattccctt tattgatcac tgacaataac atcggactga atcctagaat
      481 tcctagtaaa atagaaccga atagaagatt ttttcttgtc atgaatgttc cctccagtta
      541 tttccaaagt tgccaccatt ttttttcagt atcaatttgt ttgtctattg tttctgttgc
      601 ttcagaaaga gtcgttgagt cattctctgg agcttcttct gtataagtca actgtagttt
      661 ttcttgcagt ttttcgattt gtttgttggc ttgtagggtg agttgttgtt gctggtcgag
      721 tagtttttgt atatgagaat tattttgctc tctgagggca agttgttctc gtaaaaattc
      781 attttcttca cgcaactgat ctagcaactt gtttgtaacc acgtttttgc ttggtatatt
      841 aacattttta tcgttgtctt gttcgtactc gtgcaaccta tctagcaaca tttggttgcc
      901 taagttgcga gcaagttgca atactccaga acgcaataca accttataac cacgctcatt
      961 tttagctatc atggaagaat ctaatttttg gatatgtttc gatattgctt gcctactcat
     1021 cttaaaaagt tctgacaatt cctttattgt aaaggtttct tgtggttgca tactggttgc
     1081 ctcctaggtt gctactggaa taaattatct aaatcagata ataaactttt tttctctttt
     1141 tccgaaagtg cggactctgt ttgtttttca agagcttttt tctctttttc tagttgtttg
     1201 atttgttgga ggagttcaag ttctcgtttc ttctgtttga ttttttcgtt aattgttgta
     1261 gcgggttctt cgtattcttt ttgtttatca gctaaagcca atgctttatt ttggtcttct
     1321 ttggttggtg catgtttgtt aaagtcgtct gcttttttga tctcaggttt gaacgtaaat
     1381 tgatagccaa taacaggttt tccccgtcct tttccgtatt tctttttgat tgttagtcct
     1441 ttgaagattg cagttagttc ttgacgtata ggttttataa ctcgtttatc tatatcaggt
     1501 tgcttataac ttttaggaat atctaataat tcaagaaagt cttctttgga gaaatatgcg
     1561 aaacctttgg ttctgtattg ctttaacaga cgaaacatag tctttgcata gccactttgt
     1621 agctcgttga attgttgtaa agaatagcgt acccactcgt ctaaattatt taacaaaggc
     1681 agtgcttttt cgtaaattcg tatatctacg taaggatttt ctacgtcccc gttgatttta
     1741 aattctgtaa acattacaaa catatcgtaa gaaagcccag actcacttct acgaccaaag
     1801 cgtaaagata agattttttc ataagttgct tttaaatctt ctacaaatcg tacatttgca
     1861 gttgctttgt agttacttaa atcttttagt tggtcaaacg taaaacgaac tgtcttatcc
     1921 cctttttcac gcattctaga cacgatggaa aaaaacaaat tcatttcaac cggtgtaaat
     1981 tttcttaaag gaattgtatt taattcatgg tggtatttta caatctcatt agccatataa
     2041 tcacctcaat ccaattatga atgacatttt ttaaatgtca agacattcat atttgacgtt
     2101 caaacagaca aaatgtcctt cataaacaga caaaatgtcc ttcataaaca gacaaaatgt
     2161 ccttcataaa cagacaaaat gtccttcata acattctgta tccattggta gagtaaggac
     2221 acagcttacg gtaaagaggt ttaaaaagag gtttaaaaag agatataaaa agaggggggc
     2281 taattttccc gcctaaaaac cacttctttt ttctttaaac aggttcgcgc atttttcaaa
     2341 actgccaatc caaaaccttg cttcctacgg tcgccaaaaa ggtgtgatcg ctacgctctc
     2401 acattgtact gcgcagtcgc ctaaaggctc ctttgctaaa aacatgttgg taaagaggcc
     2461 tttgagcggt tctgaatgct tatctaagcc gttttaagac agtcttggat aaaaatcctt
     2521 atctagcttt aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc taaaataagc
     2581 gatacttaaa aacaccacac aagtttaatt ttttaagata taataacttg tcgcctatcc
     2641 gacagtcttg cgactggaag gaggtgtatc taatgtttga attgttcatt tgcccccttc
     2701 ttatagaagt ttgtgtctct ttattcagtt attggttgaa taaccgaaac aagaagtaaa
     2761 atcaataaag gcgacatcaa cctgaaaaaa agccccagag aatccgacct ctctggggct
     2821 ttcgtgtatc taatgtttga tcatttgcat gtttagttta acatgattgc cgatcaaatt
     2881 caattttgct gttgctgatc taaccattcc ttgacacttt ttagataatt taatgccgtt
     2941 tcttttttcg ttggattttg tatgttcgtt gcttgctgaa gcagtgcttt ataacgttcg
     3001 taggtaactg attgttgccg ttcttttcgt tcctgttcac tagttgaaaa agggattgta
     3061 tcactgaata aattataaca agcttcttct gtctgcgtat ctttaaaata ttgcacgtct
     3121 tttttggttc gctcatagtt gtcttgaacc tctttttgct gtttttctgg caagttttga
     3181 taaagacgag tgagtttttc catgttttta tccgtcaaac gtttttcttg attcacaaaa
     3241 atatcgttca tttcctcata acaagtggta aactcttcca ttttttgtgt taacacttgc
     3301 tcctttttct ttttatcaca tgctgcttca tacttttgag tgatatcccc taataaaata
     3361 cttcttgaaa tacgtttatt tccctctaaa acgccgaata aaccacttaa aacgattgat
     3421 aatgtaaata taacccctgg caataaaaac ctcttaaatc gaattttaga gccttttaat
     3481 gaatatcttg tttgataagc cttatagcta agccaaataa ttcctaaaat actggcaaaa
     3541 atgagtagat agggtaaata gaatgccatt tctatttatt ctccccatac cagccaaaag
     3601 gctaacagtg tcatgattaa gcaacataag ggggcaagaa tatccattga tcgctgcata
     3661 attacttttt tctgttcttt atttcgctgg tttaatagcg catggtggaa ttttttgact
     3721 tgtgaatcaa ttgtagtttg tattctttct ttggtataag gtgcaagaaa agcacgttgt
     3781 ttctttattt cattttctat gtcttgtaac gttttagaac gataattctt gttggtagtc
     3841 tgctcctctt tcaacgtgtt ggtcatggtt tcttcgagtc ggtctaattt ccggttgttc
     3901 agtcgtaaga gttcgttgtt ctcttcgtac ttcttttgca gtttcgcgaa tgctagctcg
     3961 agctcttcgt agctcgttgg ttcgttgttg attgtaggct cgttctcgtc tgtccagctc
     4021 atgtaaaatc gtctcctttt cgtaatcctt tcctagtttg ttggatcgta cttttttctt
     4081 tttgtggatc aattcgtaag taaaattttt cccacgattc aagcaatgaa tgccgaatgt
     4141 tttaagagaa tcacaaaatt ggttatacgt tcgagttttt ttgtttttca ttgtttgatc
     4201 aatcgcaaac cgaatttcgt ctttccaaac aggttcattt ttttctttta atttgatttc
     4261 agacatggtt cgtttttcgg gtttcttccc tcgttcagtg attgttaagt catgttcaag
     4321 acaaatctcg tcatttaggt cataacattt gtttaaaaat gcttgaccat gggcttgaaa
     4381 tttgttccca gtttccagat tgacggagtt aatgacaatg tggttatgaa tatggtcttt
     4441 gtctgtatgt gtgtaaatcg cgatttggtg gtcaggggct atttttttcg catactctgc
     4501 ccctagttga ttggcttttt cagcggtaat ttctcctggt cgaaaagact ggatcaataa
     4561 gtgtgcttgt acacgatcgt cttttccata cagcatgcga atttgtttca tttcgctttt
     4621 ggcataatca atgtcacagt ttatccctgt tttgacggtt gctcgtggtt cggcgtaatt
     4681 gatcgctcgg ctacaagaag tggttcggct tactttaact gttgccatat cttttgtagc
     4741 tccttttgca tgatttttag gttttcggat aagcgttggg cgtcgccagg atcaacgtcg
     4801 tgttggtgtg tgttgcacca cttggcaatt tgattgatat tcccgccagc tttggctaaa
     4861 tcgcgcgcga ttgcctgagc aatgtctggc gccactttcg gtgtcaccag acgcgcgttt
     4921 tgtgccttgg ctttcacgaa agccggcaca ctcatttgca aagcttcagc tgactgcttc
     4981 agctttgcaa attcggattc gctcactcga aaactgattt gttttggctc ggatcgatag
     5041 gtatattttt tagaatgatc gctagccaaa tattgatttt gttcgctcat aagtcccacc
     5101 ctttcagtac gtcctacgga cgtatcgttc gcaataccat tgtacctaaa aaggtacaat
     5161 aagtaaagct cactaaagct cactaaaggg cttggcaagc tctatatttg ctagccaaat
     5221 acctacgtat ttggtgcaaa taaccgcttg tcaaaggggc gcaagctccc ccttgaatcc
     5281 ccctcccccc cactctccac aatacaaaaa agactagcaa atgctagcca ctttttgtaa
     5341 agtttcgctt gtttcacctt cggtgaaatt gttgcaaatc aataattagg tagagctaca
     5401 tttttttcgt tgttcaaaga ttcttatgcc atttactatt aataaatcta taaatatgat
     5461 aatttttcgg ctctataatt tatgggactg atgaatcaga attgattctt cagtcccatt
     5521 ttcattttat tccaaagaat taactccaaa taccaataga agcccatcct ctatatactt
     5581 gagattcctt actaacaatt tcttgtaatt ctattaaaga tttaggtaat ccaaatttat
     5641 gttttaaaat atatcttcca agaaaactac ttaatttcat actaacttca ccaacacttt
     5701 ttccctcact gatttcttta gaataaagaa agagcttttc ttttaagtca ggatagtcat
     5761 ctatagttgc ttcattgtat actttactaa ttgcatttaa aagtttttct tctttagtaa
     5821 aatccattat aataacctcc ctaccactac cttctaggtg cccatggacc atgattaacc
     5881 caaccattaa caataatttt tccaatttct cctttagctt gattccaatc tacccaacat
     5941 ttttctttgt tacaataaag accatttcca tagtaagtag ctgcatcaac ttttattcct
     6001 gtaccgatgc cagctagaca agttcctaat attcctagaa taacacaatg ttttaatact
     6061 ttctttttca tgattgtttt tccttttcgt ttttttaaaa ttgcttgtta tattaaataa
     6121 tcttagtaat tttattatat aacattttat tatataacaa tga
//
