LOCUS       AP031227                7067 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE3 plasmid pNUITMV13_5 DNA, complete
            sequence.
ACCESSION   AP031227
VERSION     AP031227.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639532
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 76x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE3"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV13_5"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            838..963
                     /locus_tag="NUITMVRE3_31350"
     CDS             838..963
                     /locus_tag="NUITMVRE3_31350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94432.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSVNYSPLS"
                     /name="hypothetical protein"
     gene            complement(1100..2248)
                     /gene="tnpB_12"
                     /locus_tag="NUITMVRE3_31360"
     CDS             complement(1100..2248)
                     /gene="tnpB_12"
                     /locus_tag="NUITMVRE3_31360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287525.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="BFG94433.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB_12"
     gene            complement(2265..2669)
                     /gene="tnpA_9"
                     /locus_tag="NUITMVRE3_31370"
     CDS             complement(2265..2669)
                     /gene="tnpA_9"
                     /locus_tag="NUITMVRE3_31370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287522.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="BFG94434.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA_9"
     gene            2807..3262
                     /locus_tag="NUITMVRE3_31380"
     CDS             2807..3262
                     /locus_tag="NUITMVRE3_31380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94435.1"
                     /translation="MGDFCLILLNENNATENVVIEYTEDGMVVTKYEDGYSAEDFANSS
                     DIMTRAAVARWGKKEYTNIAVTTGVAANAINAAFWAGGKASTSMFGIPGWAIGALLTGA
                     SWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGYDAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            3280..3477
                     /locus_tag="NUITMVRE3_31390"
     CDS             3280..3477
                     /locus_tag="NUITMVRE3_31390"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94436.1"
                     /translation="MISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQLYK
                     SGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            4667..5635
                     /locus_tag="NUITMVRE3_31400"
     CDS             4667..5635
                     /locus_tag="NUITMVRE3_31400"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BFG94437.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            5652..6176
                     /locus_tag="NUITMVRE3_31410"
     CDS             5652..6176
                     /locus_tag="NUITMVRE3_31410"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFG94438.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            6552..6833
                     /locus_tag="NUITMVRE3_31420"
     CDS             6552..6833
                     /locus_tag="NUITMVRE3_31420"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390111.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94439.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     Typing          4668..5631
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atactggtgt tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg
       61 aaaatcaact caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc
      121 aaacctttac ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac
      181 cgatccctaa aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca
      241 agttggtacc aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta
      301 ttttgaggcg ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga
      361 ataggacgta ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat
      421 cctattcatc aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat
      481 atcaaatcct ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa
      541 ctaaaaaaga gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt
      601 aaaaatcaca aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtcgaca
      661 ttatgtcaac aagaatttaa tataacatat tttcctaatg cattctaact ataccacatt
      721 atcaaaaata taaattatct ttactaaata aaatattgac aagcctatac cttttgtttt
      781 atgattttca caaagaaaac gttttctata tatttttctg tatcaggagg aaattctatg
      841 aaaaaattta ctttaggggt agctttgtta actattgggg cagttgtttt accgtcaact
      901 actatagttt atgcttcaga aaattcccaa gtggtatcag tgaactactc accacttagc
      961 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
     1021 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
     1081 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
     1141 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
     1201 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
     1261 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
     1321 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
     1381 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
     1441 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
     1501 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
     1561 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
     1621 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
     1681 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
     1741 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
     1801 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
     1861 tgatgaattt actatcaatc aaagcaattg taccattttg attattcgtt gtataactct
     1921 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1981 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     2041 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     2101 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     2161 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     2221 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     2281 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     2341 caaaagcttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     2401 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     2461 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     2521 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     2581 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     2641 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     2701 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     2761 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcgacttctg
     2821 cctaattctg ttaaacgaaa acaatgctac tgaaaatgta gttattgagt acactgaaga
     2881 tggtatggtc gtaactaaat atgaggatgg ttatagtgcg gaagattttg caaattcttc
     2941 agatatcatg acaagagctg cagttgcaag atggggcaaa aaggaatata caaatattgc
     3001 tgtaaccact ggagtagctg ctaatgctat taatgctgcc ttttgggcag gagggaaagc
     3061 tagcacttct atgtttggta tcccaggttg ggcaattggt gctttgttaa caggtgcaag
     3121 ttggactaaa aaaggtgctt ctccagggaa tgcagttgca aaagaatggg acaaaaatgg
     3181 taatggctgg attggatttt attaccaaag gggatatgat gcagctggaa gagttgtagc
     3241 tactagatat tcaacgttat aagttaagga gtgatgtttt tgatatctaa atcattaaaa
     3301 tcattaaaaa catttactaa aaagaatccg caaatagcta ctattcgtat ttttatattt
     3361 gctatagctg ttgctataat tgttaatatg ctaagcggag ggcaattata caaatctggt
     3421 ttatttttag tgttaatctt agttgtctta gacataataa agtctaaaaa aaaataaatc
     3481 gatacatttt agaagaatag ggttgtttga atattacaaa gcaacaattt caccttcggt
     3541 gaaataagcg aaactttaca aaaagtggct agcaaatgct agcctttttt tgtattgtgg
     3601 agcgtggatt ttgaggggga ttcaaggggg agcttgcgct cctttgacaa gcggttattt
     3661 gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaacac tttagtgagt
     3721 tttgtagaaa gtacctttaa aggtactttc acattacgaa cgatacgtcc gtaggacgta
     3781 ctgaaagggt gggatttatg agcgaactaa tgcaaaattt ggctagcaaa aaaagttaaa
     3841 aaatatatgc taataatcga gcgaaatgaa cactttttca agtgagcgaa tccgccatga
     3901 caaagctgaa gcagtttatt tcagctttgt catggcgtgt gcctagactt ttaaaaaagt
     3961 cggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg tttgaaacgt
     4021 ctaagtatat aaatatataa gagtaggttt aagagtgctt aaatcgaatt ttagagggca
     4081 ttcagacaac tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaagaac
     4141 aagataaggg atttatacta agactgtctt aaaacgtctc aaacgaaggc tcatagcttc
     4201 tcaaagagtt ccttatgaac atttttagca aaggagccgg aagcgactgc gcagtataat
     4261 gtgagagcag agcgatcaca cctttttaag cgactgtagg gagtaagatt cccagttgag
     4321 aaaaatgcgc gaatctgttt ggagaaaaaa gaagtggttt tttaggcggg aaattttgtc
     4381 cccctctttt ataatctctt tttaaacctc tttttaaacc tctttaccgt cggctatatc
     4441 cttactctcc caagggaatg acgcttttat ctacatcgtt ttgtctgttt atctacatcg
     4501 ttttgtctgt ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttgtac
     4561 attgaattag tacatcgacg atgttaatat aagatgtaga taaacaggca ttttgtattt
     4621 cataacgtgt atctcttgtg cgagtaagaa aggagctatt ttaaatatgt ctaatgaaat
     4681 cgtaaagtat catcatgaat taaatacaat tccgttgcga aaatttacac cagtcgaaat
     4741 gaatttgttc ttttctatcg tatcaagaat gcgtgatgta ggtgataaaa aagtacaatt
     4801 tacattcgag caattaaaag atttaagcaa ttataaagca actgcaaatg ttcgattcat
     4861 agatgattta gaaacaacgt atgacaaatt aatggattta cgttttggta gaagaagtgc
     4921 tgacggtttg caacgtgaac gttttgtttt atttaatcaa tttaaaatcg acggaaaatc
     4981 tgatattcct tttgctgaaa tacaagttca tgaaaaagcc ttacccttac ttaataatct
     5041 tgaagaatgg gtgcgctatt ctctccaaca attcaatgaa ttagaaagca gttattcaaa
     5101 aacaatgttt cgattactaa aacaatacag aaccaaaggt ttcgcttatt tttcaaaaga
     5161 agactttcat gaattacttg atattcctaa aagttacaac caaggtacga ttgatcaacg
     5221 tgtgttcaat ccaatacgac aagaactaac cgcaatcttt aaaggtttaa caatcaaaaa
     5281 gaaatacgga aaaggacggg gaaagcctgt cattggctat caatttacat ttaaacctga
     5341 gataaaaaaa tcagacgact ttaacaaaca tgccccaaca aaagaagaac aaaacaaagc
     5401 attagaacta gctaaccaac aagttcaatc tgaaaaagca cctacttcat tacaagaaaa
     5461 aatcaaagaa caaaagcgaa aattggaact tttacaacag ttagaaaagt tagaaagaga
     5521 acaaaatacc caaaataaag aaaataatgc acaaaaatca gagataaatt ccgatattcc
     5581 ttcagaacta aaaaaagact tattagacaa tcttcaaaac ttattcaaag attgaggggt
     5641 ttttagattt tatgacagaa aaaacaatca aagaacttgc tgaagaatta ggagttagta
     5701 aaacagcaat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt tcgaaaatcg
     5761 gaaacaagtt tgtgattaat gaaaccggac aaaaggctat aaaatcaatg ttttttaacg
     5821 aaaacgaaaa ctcaaatcaa caactagttg gcgaaaaaac gaaaaccgaa aaccacaaaa
     5881 acgccaacca agttcccgaa ttagaagtcc tttctctcct agaacaacag ttaaatcaaa
     5941 aagatatgca actacaagaa aaagacgagc aaataaaaca aatgcaaaaa ttactcgacc
     6001 accaacaaca actcaccctg caagccaacc aacaaatcga gaaactgcag gaacaactac
     6061 agttgactta tacggaagaa tcttcggaaa ataaatcaac gactctttct gaagaaacag
     6121 aatcgataga aaaacaatca aaggcggaga aaaaatggtg gcaattctgg caataaaacc
     6181 agcaatttaa attaataaaa attgctggtt ttattcttct ttttgctttt gcgagcagag
     6241 caacatggac ccatgttgcg atattttggt ggcgccccaa tccccgtttt ttcgttgaat
     6301 cttactcgga ctgcactcgt tttacagctg atttttctga attttgaaag agcataagga
     6361 aacgtggtat cactttctta cgaaaggtgc tagataggct gtgcctatca cgtttctcag
     6421 aaaaaaattt gctatcactt ttttgccatt gtggctaggc tatgtatagt gttttggcga
     6481 aaaatagcta tacaaatact atatttgtat ggtatacata aattatacgc tgatacgaaa
     6541 ggaaaatttc catggacatt ttagttcgaa atatcaatcc caaaattatt gcaagaatag
     6601 ataaacgttg tcaggaactc acaaccaaaa cgggcaaaaa atggagtcga aataaatatc
     6661 taaaagttct aatcgaaagt gattttgacc gtcaacttat gaattacaaa aaagatcaat
     6721 tcgatttatt actcgaaaaa tttattgcca ttcaaacaga aaatacgaac atactgaatg
     6781 actatgttca cacaaacaac caactcattg aaacacttat cggaggaata taacatgaga
     6841 aaaacttctg ttcaaaaaca cgtaaccctt tatcccagtc acgttgacgt tttagattcc
     6901 atcatcgaaa aaaatccaga cgtcaacaat tattcagaag ccattcgttt tttatgtatg
     6961 agttatgaac ctgataccaa tacaaccaca accagtaacc aagcaaaact caatgccatg
     7021 ggaaaagaaa tttctatcat gacagaaatc atggcagaat ttgctga
//
