LOCUS       AP031226               10833 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE3 plasmid pNUITMV13_4 DNA, complete
            sequence.
ACCESSION   AP031226
VERSION     AP031226.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639532
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10833)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 76x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..10833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE3"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV13_4"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            <1..503
                     /locus_tag="NUITMVRE3_31230"
     misc_feature    <1..503
                     /locus_tag="NUITMVRE3_31230"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="partial CDS similar to WP_000145511.1:replication
                     initiation protein"
     gene            514..714
                     /locus_tag="NUITMVRE3_31240"
     CDS             514..714
                     /locus_tag="NUITMVRE3_31240"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94421.1"
                     /translation="MILTKFQKNRLKFRLFFLRINGQEPKRSAKKTYQAPQAKKVRVTS
                     KKEKFFRTTFENLILIYIRSI"
                     /name="hypothetical protein"
     gene            791..964
                     /locus_tag="NUITMVRE3_31250"
     CDS             791..964
                     /locus_tag="NUITMVRE3_31250"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94422.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            1370..1804
                     /locus_tag="NUITMVRE3_31260"
     CDS             1370..1804
                     /locus_tag="NUITMVRE3_31260"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94423.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="hypothetical protein"
     gene            3094..3915
                     /locus_tag="NUITMVRE3_31270"
     CDS             3094..3915
                     /locus_tag="NUITMVRE3_31270"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117494.1"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="BFG94424.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEAD"
                     /name="RepB family plasmid replication initiator protein"
     gene            4034..4573
                     /locus_tag="NUITMVRE3_31280"
     CDS             4034..4573
                     /locus_tag="NUITMVRE3_31280"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFG94425.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            5281..5454
                     /locus_tag="NUITMVRE3_31290"
     CDS             5281..5454
                     /locus_tag="NUITMVRE3_31290"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94426.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            5860..6294
                     /locus_tag="NUITMVRE3_31300"
     CDS             5860..6294
                     /locus_tag="NUITMVRE3_31300"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94427.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="hypothetical protein"
     gene            7584..8507
                     /locus_tag="NUITMVRE3_31310"
     CDS             7584..8507
                     /locus_tag="NUITMVRE3_31310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94428.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="hypothetical protein"
     gene            8523..9062
                     /locus_tag="NUITMVRE3_31320"
     CDS             8523..9062
                     /locus_tag="NUITMVRE3_31320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BFG94429.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            9770..9943
                     /locus_tag="NUITMVRE3_31330"
     CDS             9770..9943
                     /locus_tag="NUITMVRE3_31330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94430.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            10349..10753
                     /locus_tag="NUITMVRE3_31340"
     CDS             10349..10753
                     /locus_tag="NUITMVRE3_31340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFG94431.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNNSYSIYLSNF"
                     /name="hypothetical protein"
     Typing          2..504
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.802"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3095..4018
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.676"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7585..8507
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaagaactg gacaagattt aatctagagc aatttactaa tttggatagc acatattcaa
       61 aaactatgtt tcgattatta aaacaatatc gaactcaagg ctggactgaa ttttccaaag
      121 aaaaattttt tgatttacta gatattccta aaagttattg gaatagtccg tcaaatattg
      181 acaaatttat cttaaggcca attaaagaag aactcactcc cttatttaaa ggattaacta
      241 ttcgtaaaaa atatggtaaa ggtcgaggta aacctgttat cggttatcgt tttacttgga
      301 aagctgaaat taaccatgct gacgattttt ccaaagggaa acaagaagat ttacgtataa
      361 aattatttaa tattgatcac aatggagaat taacacgaga agaaaaatgg cgcgctaaag
      421 atcgaatact aaatcttccc ttaggcactc atgaagctga ttttaaacaa caacaacaaa
      481 ctgaagaaaa acaggcagaa taaaaaaaca aatgtgattt taacaaaatt tcaaaaaaat
      541 cgtttgaaat ttcgtttatt ttttttgaga attaatggtc aagaacctaa acggtcggcg
      601 aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa aaaagaaaaa
      661 ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat ttaaacattt
      721 gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa aaaaagtgga
      781 ggtataacct atgttaaaaa aaactgtgtt ggtactgggt tctatttctc taggaatatt
      841 aggttttagc cccatgctat tatccgtaat tggaaaaaac aattccaacg gagaacttaa
      901 attaaattca aattattaca acagtcctaa cgaagaattg tttgtaaaag agacggataa
      961 ataataaaaa agactgctct ttcgagcagc cagttaaaaa aataatgaga gtttattctt
     1021 gaaaaagcaa cgcgcttata aagcgctacc aaaaatttac catattttat gaaaaataac
     1081 aacccagtaa aaaatgactg gtaaaaattc acaaagaaac ggaaagaagc aactgcagaa
     1141 aatcaagcct taaaaagagc tttttaccta gaccaattaa aaatcacaaa gtgagcccct
     1201 gtatcggttg atacaagggc tacttggcat actcgacatt atgtctacta acctttaaaa
     1261 taacacgttt tataaaataa accctcttga caatatttaa ttttctgatt attctaaata
     1321 tatacagaaa gcgctttcct aggattttat atctaaggag gaagtaacaa tgaaaaataa
     1381 aattattaaa attattactt gtctattttt gttcggtatg attgttggag caagtactcc
     1441 gttaaatctg tttcctttta gtgctagagg tataaaagtg gaagctgcaa gttcacgata
     1501 taatcataat catcgtggat ttacttgcta taaaatgaac tattatgtta ctaataaaat
     1561 gtgtagagat ttgaaaaaaa attataataa gctaaagact cctgctcaaa ttgcatcttt
     1621 tattcctatt ggtggagttg gaacatgggt tattacaaat gcttttagta gtgctgttga
     1681 tgcaatgaat gtctttgtaa gagctgcaaa tcaaggaaaa ggtgtacaat taacatataa
     1741 tgctcacttt agcaatacaa catcatatca atacaatgat tacgctcgtt atgtaataaa
     1801 ataatcagaa agtgaaacta tctcatgaat aaaaagattt tagctgtaat tatttcaata
     1861 atagttttaa tcactatgtt cttcatattt aggctaattt tcaatataag cttacaaaaa
     1921 agtattcttt atctaattcc tatagcattt atcttagcaa tttttagaag tatttacgga
     1981 aataaaaact ctaaatagct aaaaataaat catactaaat tttacaaaaa gtggctagca
     2041 ttttctagcc actttttgta ttgtggagcg tgggttttga gggggattca agggggagct
     2101 tgcgcccctt tgacaagcgg ttatttgcac caaatacgga ggtatttggc tagcaaatat
     2161 agagcttgcc aaaccctttt agtgagtttt gtagaaagta cctttaaagg tactttcaca
     2221 ttacgaacga tacgtccgta ggacgtactg aaagggtggg acttatgagc aaattaatgc
     2281 aaaatttggc tagcaaaaaa gctaaaaaat atatgctaat aatcgagcga aataaacact
     2341 ttttcaagtg agcgaatccg ccatgacaaa gctgaagcag tttatttcag ctttgtcatg
     2401 gcgtgtgccg acttttttaa aagtctaggc acacgagcaa atatctttat caagctctaa
     2461 tttgcgtttt aagacgtttg aaacgcctaa gtatataaat atataagaat aggtttaaga
     2521 gtgcttaaat cgaattttag agggcattta gacaatttta gggttgttag ggcttgccct
     2581 gaccgcctgt aaggcgtttt aagaacaaga taagggattt atactaggac tgtcttaaaa
     2641 tgtcttaaac gaacgctcag agcttctcaa agggttcctt atgaacggct tttagcaaag
     2701 gagccggagg cgactgcgca gtataatgtg agagcagagc gatcacacct ttttagcgaa
     2761 ccgtagggag caaggtttgg aattggcagt tttgataaat tcgcgaacct gtttagagaa
     2821 aaaaggagtg attttttagg cgggaaaatt agcctcccct ctttttatat ctctttttat
     2881 atctcttttt atatctcttt tatgtcggct gtgtccttac tctcccaagc aattcagagt
     2941 tttttcaagt aggttttcta cgcttttcaa gtaggttttc tacgcttttc aagtaggttt
     3001 tctacgcttt tcaagtaggt tttccgacat tattgagtat ttttataaag tatgttactt
     3061 ttatttcata ctttataaaa gtaggagaag cttatggcaa atgaaattgt aaaatatgaa
     3121 aatagattaa atcatattcc tctgagaaaa tttaatagtc gtgaaatgaa cttatttttt
     3181 tcaattgctt cacgtgtacg tgaaaaaggt gtagatgaga ttattttttc atttcacgat
     3241 ttgaaaaaac taagcggtta tacagaacgt ggggaacaat ttataaaaga tcttagtagc
     3301 acgtatgaca aattattaag tattaatgct tggaatgatg atggaaatac tttaactaaa
     3361 tttgttgctt tcacaaaata tagcattata cgagataaac aacttgttat aatttcagtt
     3421 aatccagaat ttaaaggact ttttaatcaa ttaaagaact ggacaagatt taatctagag
     3481 caatttacta atttggatag cacatattca aaaactatgt ttcgattatt aaaacaatat
     3541 cgaactcaag gctggactga attttccaaa gaaaaatttt ttgatttact agatattcct
     3601 aaaagttatt ggaatagtcc gtcaaatatt gacaaattta tcttaaggcc aattaaagaa
     3661 gaactcactc ccttatttaa aggattaact attcgtaaaa aatatggtaa aggtcgaggt
     3721 aaacctgtta tcggttatcg ttttacttgg aaagctgaaa ttaaccatgc tgacgatttt
     3781 tccaaaggga aacaagaaga tttacgtata aaattattta atattgatca caatggagaa
     3841 ttaacacgag aagaaaaatg gcgcgctaaa gatcgaatac taaatcttcc cttaggcact
     3901 catgaagctg attaaacaac aacaacaaca aactgaagaa aaacaggcag aaaaacgaga
     3961 actttcaaac aaactcaaac aagatttact aggaaattta caaaacttat tcgattaagc
     4021 ttaaggagta tttatggcag ataaaacaat caaagaactt gcggaagaat taggagttag
     4081 taaaacagca attaacaaaa aagtttctga caaaaatcga aaactttggt ttgcaaaaaa
     4141 cggaaacaag tttgtgatta acgaaaccgg tcaaaaagct ataaaatcaa tgtttttagt
     4201 cgataacgca aacttaaatc gaaaactagt tggcgaaaaa tcaaaaacca aaaaccataa
     4261 aaacaccaac caagtttccg aattaaaact cctttctttc ctagaaaaac agttaaatcg
     4321 aaaagatata cagctacaag aaaaagataa tcaaataaaa caaatgcaaa aattactcga
     4381 ccagcaacaa caactcactc tacaagcgaa ccaacaaatt gagaacctac aagaacagct
     4441 ccatttaact tatacagaag aaactccaac aaccgaatca actgcctttt ctgaaaaaaa
     4501 agcagacaat acacaaccac ttattgaaaa aaagtggtgg caaatttgga aatctaatag
     4561 aaccaatctc taaaattttc tagaagttag ttcggatagt agaggaaaaa gttgtactac
     4621 ctgatgggga gatcactcaa atttttatga gaaagaaaaa atagtttaca actgtaaaca
     4681 atcaatgttt atagttgtaa acaatcaatg tttatagttg taaactatca aactatatgg
     4741 tgacttattg cttaaaatag gttgaaatct cactcaattt ttggtggaaa aacaaaataa
     4801 attctaatcg attttgttga aaaagtcata aaaaaacaaa tgtgatttta acaaaatttc
     4861 aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat tttgatcatc gtagggtcat
     4921 tgtagaatca ttatgagatc atcgtgggat catcgaataa agtatgggaa aggcttgcgg
     4981 tgcttggtat taagggcatt ctccgcctcc caggttcttg accattttct ctcaacgtgg
     5041 taaaaatccc acttataaga gaaaatggtc aagaacctaa acggtcggcg aagaaaacat
     5101 accaagcacc acaagcaaaa aaagttagag taacttccaa aaaagaaaaa ttttttagaa
     5161 caacttttga aaatttaatt ttgatttata tccgttcaat ttaaacattt gataaactga
     5221 aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa aaaaagtgga ggtataacct
     5281 atgttaaaaa aaactgtgtt ggtactgggt tctatttctc taggaatatt aggttttagc
     5341 cccatgctat tatccgtaat tggaaaaaac aattccaacg gagaacttaa attaaattca
     5401 aattattaca acagtcctaa cgaagaattg tttgtaaaag agacggataa ataataaaaa
     5461 agactgctct ttcgagcagc cagttaaaaa aataatgaga gtttattctt gaaaaagcaa
     5521 cgcgcttata aagcgctacc aaaaatttac catattttat gaaaaataac aacccagtaa
     5581 aaaatgactg gtaaaaattc acaaagaaac ggaaagaagc aactgcagaa aatcaagcct
     5641 taaaaagagc tttttaccta gaccaattaa aaatcacaaa gtgagcccct gtatcggttg
     5701 atacaagggc tacttggcat actcgacatt atgtctacta acctttaaaa taacacgttt
     5761 tataaaataa accctcttga caatatttaa ttttctgatt attctaaata tatacagaaa
     5821 gcgctttcct aggattttat atctaaggag gaagtaacaa tgaaaaataa aattattaaa
     5881 attattactt gtctattttt gttcggtatg attgttggag caagtactcc gttaaatctg
     5941 tttcctttta gtgctagagg tataaaagtg gaagctgcaa gttcacgata taatcataat
     6001 catcgtggat ttacttgcta taaaatgaac tattatgtta ctaataaaat gtgtagagat
     6061 ttgaaaaaaa attataataa gctaaagact cctgctcaaa ttgcatcttt tattcctatt
     6121 ggtggagttg gaacatgggt tattacaaat gcttttagta gtgctgttga tgcaatgaat
     6181 gtctttgtaa gagctgcaaa tcaaggaaaa ggtgtacaat taacatataa tgctcacttt
     6241 agcaatacaa catcatatca atacaatgat tacgctcgtt atgtaataaa ataatcagaa
     6301 agtgaaacta tctcatgaat aaaaagattt tagctgtaat tatttcaata atagttttaa
     6361 tcactatgtt cttcatattt aggctaattt tcaatataag cttacaaaaa agtattcttt
     6421 atctaattcc tatagcattt atcttagcaa tttttagaag tatttacgga aataaaaact
     6481 ctaaatagct aaaaataaat catactaaat tttacaaaaa gtggctagca ttttctagcc
     6541 actttttgta ttgtggagcg tgggttttga gggggattca agggggagct tgcgcccctt
     6601 tgacaagcgg ttatttgcac caaatacgga ggtatttggc tagcaaatat agagcttgcc
     6661 aaaccctttt agtgagtttt gtagaaagta cctttaaagg tactttcaca ttacgaacga
     6721 tacgtccgta ggacgtactg aaagggtggg acttatgagc aaattaatgc aaaatttggc
     6781 tagcaaaaaa gctaaaaaat atatgctaat aatcgagcga aataaacact ttttcaagtg
     6841 agcgaatccg ccatgacaaa gctgaagcag tttatttcag ctttgtcatg gcgtgtgccg
     6901 acttttttaa aagtctaggc acacgagcaa atatctttat caagctctaa tttgcgtttt
     6961 aagacgtttg aaacgcctaa gtatataaat atataagaat aggtttaaga gtgcttaaat
     7021 cgaattttag agggcattta gacaatttta gggttgttag ggcttgccct gaccgcctgt
     7081 aaggcgtttt aagaacaaga taagggattt atactaggac tgtcttaaaa tgtcttaaac
     7141 gaacgctcag agcttctcaa agggttcctt atgaacggct tttagcaaag gagccggagg
     7201 cgactgcgca gtataatgtg agagcagagc gatcacacct ttttagcgaa ccgtagggag
     7261 caaggtttgg aattggcagt tttgataaat tcgcgaacct gtttagagaa aaaaggagtg
     7321 attttttagg cgggaaaatt agcctcccct ctttttatat ctctttttat atctcttttt
     7381 atatctcttt tatgtcggct gtgtccttac tctcccaagc aattcagagt tttttcaagt
     7441 aggttttcta cgcttttcaa gtaggttttc tacgcttttc aagtaggttt tctacgcttt
     7501 tcaagtaggt tttccgacat tattgagtat ttttataaag tatgttactt ttatttcata
     7561 ctttataaaa gtaggagaag cttatggcaa atgaaattgt aaaatatgaa aatagattaa
     7621 atcatattcc tctgagaaaa tttaatagtc gtgaaatgaa cttatttttt tcaattgctt
     7681 cacgtgtacg tgaaaaaggt gtagatgaga ttattttttc atttcacgat ttgaaaaaac
     7741 taagcggtta tacagaacgt ggggaacaat ttataaaaga tcttagtagc acgtatgaca
     7801 aattattaag tattaatgct tggaatgatg atggaaatac tttaactaaa tttgttgctt
     7861 tcacaaaata tagcattata cgagataaac aacttgttat aatttcagtt aatccagaat
     7921 ttaaaggact ttttaatcaa ttaaagaact ggacaagatt taatctagag caatttacta
     7981 atttggatag cacatattca aaaactatgt ttcgattatt aaaacaatat cgaactcaag
     8041 gctggactga attttccaaa gaaaaatttt ttgatttact agatattcct aaaagttatt
     8101 ggaatagtcc gtcaaatatt gacaaattta tcttaaggcc aattaaagaa gaactcactc
     8161 ccttatttaa aggattaact attcgtaaaa aatatggtaa aggtcgaggt aaacctgtta
     8221 tcggttatcg ttttacttgg aaagctgaaa ttaaccatgc tgacgatttt tccaaaggga
     8281 aacaagaaga tttacgtata aaattattta atattgatca caatggagaa ttaacacgag
     8341 aagaaaaatg gcgcgctaaa gatcgaatac taaatcttcc cttaggcact catgaagctg
     8401 attttaaaca acaacaacaa actgaagaaa aacaggcaga aaaacgagaa ctttcaaaca
     8461 aactcaaaca agatttacta ggaaatttac aaaacttatt cgattaagct taaggagtat
     8521 ttatggcaga taaaacaatc aaagaacttg cggaagaatt aggagttagt aaaacagcaa
     8581 ttaacaaaaa agtttctgac aaaaatcgaa aactttggtt tgcaaaaaac ggaaacaagt
     8641 ttgtgattaa cgaaaccggt caaaaagcta taaaatcaat gtttttagtc gataacgcaa
     8701 acttaaatcg aaaactagtt ggcgaaaaat caaaaaccaa aaaccataaa aacaccaacc
     8761 aagtttccga attaaaactc ctttctttcc tagaaaaaca gttaaatcga aaagatatac
     8821 agctacaaga aaaagataat caaataaaac aaatgcaaaa attactcgac cagcaacaac
     8881 aactcactct acaagcgaac caacaaattg agaacctaca agaacagctc catttaactt
     8941 atacagaaga aactccaaca accgaatcaa ctgccttttc tgaaaaaaaa gcagacaata
     9001 cacaaccact tattgaaaaa aagtggtggc aaatttggaa atctaataga accaatctct
     9061 aaaattttct agaagttagt tcggatagta gaggaaaaag ttgtactacc tgatggggag
     9121 atcactcaaa tttttatgag aaagaaaaaa tagtttacaa ctgtaaacaa tcaatgttta
     9181 tagttgtaaa caatcaatgt ttatagttgt aaactatcaa actatatggt gacttattgc
     9241 ttaaaatagg ttgaaatctc actcaatttt tggtggaaaa acaaaataaa ttctaatcga
     9301 ttttgttgaa aaagtcataa aaaaacaaat gtgattttaa caaaatttca aaaaaatcgt
     9361 ttgaaatttc gtttattttt tttgagaatt ttgatcatcg tagggtcatt gtagaatcat
     9421 tatgagatca tcgtgggatc atcgaataaa gtatgggaaa ggcttgcggt gcttggtatt
     9481 aagggcattc tccgcctccc aggttcttga ccattttctc tcaacgtggt aaaaatccca
     9541 cttataagag aaaatggtca agaacctaaa cggtcggcga agaaaacata ccaagcacca
     9601 caagcaaaaa aagttagagt aacttccaaa aaagaaaaat tttttagaac aacttttgaa
     9661 aatttaattt tgatttatat ccgttcaatt taaacatttg ataaactgaa ggctagtatt
     9721 taaaatgtgt atacaagtgg tagcgttaaa aaaagtggag gtataaccta tgttaaaaaa
     9781 aactgtgttg gtactgggtt ctatttctct aggaatatta ggttttagcc ccatgctatt
     9841 atccgtaatt ggaaaaaaca attccaacgg agaacttaaa ttaaattcaa attattacaa
     9901 cagtcctaac gaagaattgt ttgtaaaaga gacggataaa taataaaaaa gactgctctt
     9961 tcgagcagcc agttaaaaaa ataatgagag tttattcttg aaaaagcaac gcgcttataa
    10021 agcgctacca aaaatttacc atattttatg aaaaataaca acccagtaaa aaatgactgg
    10081 taaaaattca caaagaaacg gaaagaagca actgcagaaa atcaagcctt aaaaagagct
    10141 ttttacctag accaattaaa aatcacaaag tgagcccctg tatcggttga tacaagggct
    10201 acttggcata ctcgacatta tgtctactaa cctttaaaat aacacgtttt ataaaataaa
    10261 ccctcttgac aatatttaat tttctgatta ttctaaatat atacagaaag cgctttccta
    10321 ggattttata tctaaggagg aagtaacaat gaaaaataaa attattaaaa ttattacttg
    10381 tctatttttg ttcggtatga ttgttggagc aagtactccg ttaaatctgt ttccttttag
    10441 tgctagaggt ataaaagtgg aagctgcaag ttcacgatat aatcataatc atcgtggatt
    10501 tacttgctat aaaatgaact attatgttac taataaaatg tgtagagatt tgaaaaaaaa
    10561 ttataataag ctaaagactc ctgctcaaat tgcatctttt attcctattg gtggagttgg
    10621 aacatgggtt attacaaatg cttttagtag tgctgttgat gcaatgaatg tctttgtaag
    10681 agctgcaaat caaggaaaag gtgtacaatt aacatataat aattcctata gcatttatct
    10741 tagcaatttt tagaagtatt tacggaaata aaaactctaa atagctaaaa ataaatcata
    10801 ctaaatttta caaaaagtgg ctagcatttt cta
//
