LOCUS       AP029166                1549 bp    DNA     circular BCT 23-MAY-2024
DEFINITION  Escherichia coli KA0011 plasmid pKA0011-3 DNA, complete sequence.
ACCESSION   AP029166
VERSION     AP029166.1
DBLINK      BioProject: PRJDB17347
            BioSample: SAMD00731524
            Sequence Read Archive: DRR529737, DRR529738
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Nomoto,R., Nakanishi,N., Komatsu,S., Matsui,M. and Suzuki,S.
  TITLE     Complete Genome Sequences of Intermediate Drug Resistance
            Escherichia coli KA0011 Clinical Isolate used as a Quality Control
            Reference Strain in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Nomoto,R. and Nakanishi,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2024) Contact:Ryohei Nomoto Kobe Institute of
            Health, Department of Infectious Diseases; 4-6-5
            Minatojima-nakamachi, Chuo-ku, Kobe, Hyogo 650-0046, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8
            Genome Coverage       :: 85x
            Sequencing Technology :: Illumina MiSeq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KA0011"
                     /serovar="O25:H4"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pKA0011-3"
                     /geo_loc_name="Japan:Hyogo"
                     /collection_date="2018-03-09"
     gene            1..477
                     /locus_tag="KA0011_49170"
     CDS             1..477
                     /locus_tag="KA0011_49170"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000469298.1"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="BFM34063.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(504..674)
                     /locus_tag="KA0011_49180"
     CDS             complement(504..674)
                     /locus_tag="KA0011_49180"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFM34064.1"
                     /translation="MGVWGSPQTVSGSAQGAAGTVAARFFLENGEQKHAKDFEAAKEQC
                     RIDNHGQNVNT"
                     /name="hypothetical protein"
     Typing          1069..1167
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(1363..1471)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(709..969)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 atgtttattg attcagaaaa acgactgaaa caactttcag atgaggcaaa gaaaaacacc
       61 gaggatctcg aagaagcaaa gaaaaattca aggtttacac aggtatcccc aaaaggttgg
      121 gaacgtgttc gagagctgct gaaggatagc caaggcatat cagcactgaa gctgtactca
      181 tttttagcgg agcatatcga tcctacgtgt ggcgctgtcg ttgcggatca gcaattccta
      241 gctgaaaaac ttggagttag cagaagcaca attattcggt ggctcaatta cttagaatca
      301 aaaaatgcat tagttagaat ccccgttgct ggtaaggttt gtgcgtatgc cctcgatcca
      361 catgaagtct ggaagggata caacactacg aaaaaccatg cagcgtttgt cactaaaaca
      421 ctggtcaaca aagacggtga tattcagcgc cgaatcatgg ccatgttttc aaattgagct
      481 agcggcaggc ggacaatcag gggctacgtg ttaacgttct gaccatgatt gtctatcctg
      541 cattgctctt ttgccgcctc aaaatccttt gcgtgttttt gctccccgtt ctccagaaaa
      601 aaccgagccg ccacggttcc ggcagcgcct tgagcgctac cggaaaccgt ctggggagaa
      661 ccccagaccc ccatcgctgc aacaccgacg gtctacgacc acctaccaac cgcatggctg
      721 cctgttccgt gcaaatgggc tttgcagggg gctggcgcgg ttcctggcga cctgaaagcc
      781 cctggttagg ggtatcggcg cgcttgacct cctgagagcc tctgtaagcg tttttcgcgc
      841 cttccatgcc ctggcgggca tctaaaaccc gtttcgtgcg tctgagagct tttgagcgcg
      901 tttgaggggc tatctggcga ctcatcccca aaagaacaac accgggatca ccgacaacat
      961 gcgagaggtg atcccgctcg ccgcaagtcg atgaccgagc gtagcgagcg aatcgacgag
     1021 gaagcggaag agaaccggaa gccacattga gcacttacgc actgatgcgg ggtgtcgggg
     1081 tgaagccctg accaagtggt aatcgtatcg gcgtgcatgc gcggttatac gattacacat
     1141 cctgtcccga tttctgaggc gttttaacgg tgaacggacg gcaaaaacac gatggagctg
     1201 ttgaacgtga aaaccacgaa atctggcaaa aatcacggct ggcgaggctt gcataggagt
     1261 aagccagtat acactccgct atcgctacgt gactgggtca gggcttcgcc ccgacacccc
     1321 caaagggcgt tgcgttgcac gcaacaccct tgcctagaat agatctttta cgccgatttg
     1381 taagtggttg tttttttagt gtgtttttta tttacctgtc gcatccagga tgacacaacg
     1441 ttgtcgcatc ctggatgcga caacacgcaa caaggtgttg cacagatgtg tcatgatggc
     1501 ttagatttaa accaacgtta gtgcatggga ttttcagagg gaaaaaatc
//
