LOCUS       AP028489                3223 bp    DNA     circular BCT 26-JUL-2023
DEFINITION  Serratia marcescens CM5 plasmid pCM5_2 DNA, complete sequence.
ACCESSION   AP028489
VERSION     AP028489.1
DBLINK      BioProject: PRJDB10568
            BioSample: SAMD00247259
            Sequence Read Archive: DRR253350, DRR493235
KEYWORDS    .
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  AUTHORS   Nagano,D.S., Taniguchi,I., Ono,T., Katsura,K. and Hayashi,T.
  TITLE     Complete genome of Serratia marcescens CM5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Nagano,D.S., Taniguchi,I., Ono,T., Katsura,K. and Hayashi,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2023) Contact:Debora Satie Nagano Faculty of
            Medical Sciences, Kyushu university, Department of Bacteriology;
            3-1-1 Maidashi, Fukuoka, Fukuoka 812-8482, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8
            Genome Coverage       :: 86x
            Sequencing Technology :: MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.0 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="CM5"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="pCM5_2"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            complement(250..594)
                     /locus_tag="SMCM5_46330"
     CDS             complement(250..594)
                     /locus_tag="SMCM5_46330"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEM21631.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="hypothetical protein"
     gene            1212..1382
                     /locus_tag="SMCM5_46340"
     CDS             1212..1382
                     /locus_tag="SMCM5_46340"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEM21632.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            complement(2091..2306)
                     /locus_tag="SMCM5_46350"
     CDS             complement(2091..2306)
                     /locus_tag="SMCM5_46350"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEM21633.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="hypothetical protein"
     gene            complement(2310..2795)
                     /locus_tag="SMCM5_46360"
     CDS             complement(2310..2795)
                     /locus_tag="SMCM5_46360"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEM21634.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="hypothetical protein"
     ARG             complement(1592..1923)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(670..861)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          841..1324
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(254..573)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="8.510000000000001e-64"
     Typing          complement(1994..3222)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.019"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttatcgagtc cgggcatcag aacccgctcg aaactcgcca tcagcttttc gcgctggccg
       61 ggcggtaaat cctgttcggc aaaagacaga acgccggaag tgtacttttt ggcgtagggc
      121 gaggcgtcga taagctcccg gacttcatcc ggcttcccct gcagaacgct ggcgccgtcg
      181 cgctggcgat ctttacccag cagataatcg accggcccgc cgccgccgcc gcgcccccga
      241 ggatgaaact taacgatcat catcagcacc cttttccagt acggcatgcc gcaaccgctc
      301 gagtccggca tcgatggcca tcagcgcggc gacaatctgc acgcggtcgt gtccgcttcc
      361 accaccggcg ttaacctggc gggcaatctg gttaaggttg ttccccatgc cggcaagctg
      421 acgaagcagc gccggcgaga tcgacggaag tttgccggcg cgtgcgggct tctcgtccag
      481 gcacgtctgc cgcatccagg ccgcgagttg cttaccctcg cagcgctcca gcagacgccg
      541 gtgctcgtcc tcagtcaccc acatggtgag cattttgctg cgtttgtctg ccaatccggc
      601 ctcctgataa cgccgggacg gacgggaaat cttttcccgg tcggcacggt gttggacaag
      661 ccgcagacgc ggcagtggtt ttagcgggtt tcggggcgca gccctgaacc agtcacgtag
      721 cgctagcgga gtgtatactg gctaaacatg ttatgacgga gtggtaattt atgaaagtgc
      781 ttcatgtgac aggggaaaaa tgcggctgcc gggcgtaagt gcaaaatgca ggtggtgatg
      841 aaatgacgcc tcctcgctca ctcggtcgct acgctcctgc cgtgagactg cggcgggcgt
      901 taccagctca cagattacgg gataagcaaa tgccgctcga agcaggaaag gccaagaaaa
      961 ggatgcggag ttgccgtttt tccataggct ccgcccccct gacgagtatc acgaaaatga
     1021 cgctcaaatc agcggtggcg aaacccgatg cggcttaaag ataccaggcg tttccccctg
     1081 gtggctccct cgtgctcccc cgcattcctg cctttcggtt taccggtgtc attccgctgt
     1141 tatggccgcg gtttctcatt ccacgcctga aactcggttc cgggtaggca gttcgctcca
     1201 ggatggactg tatgcacgaa ccccccgttc agtccgacta ccacgcccgt tccggtaact
     1261 atcaacttga gtccaacccg gaaagacacg acaaatcgcc agtggcggta gccattggta
     1321 actgggttca cggagcagag attgtagaga atgcctacag agttcttgaa gtgaaagcct
     1381 gagtgcggct acactgaaag gacagtttag gtatctgcgc tccgtgaaag ccagttacca
     1441 tcggaacaaa gagttggtag ctctgaattc cggcaaacga accaccgttg gtagcggtgg
     1501 tttttttgtt tacagggaaa aagattacgc gcagaaagaa aggatctcaa ggaaatcctt
     1561 ttgctttggc caatatgctt cggtaaaaag ttaatgcgca gaagattttg aaaaaatatt
     1621 aataaccaaa actccaacgc aaataaggaa cattcctgca attgcgggcc aatcaagttt
     1681 ttgaccgcta accaaccaac cagctaggct tattagtaca attcccgcac cagaccaaat
     1741 tgcgtaagca ataccggtag gtatattttc caatgtttgg gaaagcagat aaaaggcagc
     1801 gcaataactt attgcagttc ctacagacgg ccacaggcgt gtaaatcctt ctgaatattt
     1861 cataaaggtg gtgcctatga cttcagccac aatagcccct cccaggaaaa tatatgtatt
     1921 cataaaaata cctcatgttg aaaaatttaa atatctttac ataacaacta attaatagaa
     1981 cccggcttat catagcgaag gaccatgatg ctgccactgc gatcgttcca gctccagcgc
     2041 tttctggtgc tgcaccgccc gctcctgctg ccggtgagtt tctatcgcgt tcagcctttc
     2101 gacaaccggc gcagctgctc gctcaaactc tgcacctgcc tgctcaagac cgtgacgcgc
     2161 tcgttcagcg ccgcgttctc ccgttgaata agaccagaca tcgtccgcca ttccgcgaag
     2221 gcgttctccc actcgtccag ccttttcgag tagtcctgct gtagctgctc taatgcgctc
     2281 agcaactgtt tttccagctc cgtcatgtgc tatttccccg ccagtatcat ccagctcgag
     2341 tcctctccga actgtcccgc ttccgggttc accctcacgc agcggcgtct ctgctcgccg
     2401 caggtcgaga gctgtacgcc gctgttcctc tctgacagca tggcctgcgt gctcttctgc
     2461 tcccggatgg tcgtatagtt ctcgagtatc tgctgcccct gccaccacag aatgcccgcg
     2521 ctcgacgcta tcagcagcgc ggagaccaga acgatggtca gccacgtctg gctgaccatg
     2581 cgcagcatcc ctttcgtgcg ctggctcatg gctgaggaca gcttccggtc gtggtcgagg
     2641 atggcggcgc tgattctctt ctcgctttcg ctcagttccg ctctgacaga cttctcgtgc
     2701 tcgctgaatg cggctttcag catctcgccg gtagtctgct gctgcgcttt cgatttctgc
     2761 tctaagtcct tcgccagcgt taaaagactg ttcatagatg gctcccttca gtcggatgtt
     2821 tcgccccccg tccgggtcgg caatgctgat gctgcttttt gtggtgcgca ccacctcaaa
     2881 acctgcgctt tccagcgcct cagtgacgtc ctgacgcgtt ttcagctccc cggatgaggc
     2941 aagggcaagc aggccgctcg taatcgcctg ggcggcttcc tgcttcgctt ccggcagcgc
     3001 ggaaggcgag accagcaccc gccggttctc cggcgcgttc gggtcatgca gccccagcct
     3061 gccgttcact atggtctgcc aggcatcgat gcgcggacgg tcggcgcggt cgtaatacgg
     3121 ctgtagccgc ttgccggtca gaagctccgt gttcgggatc aggaagttca gctccagccg
     3181 ccccttgtcc cggtgctcaa cccacagcac gctgtactgg tct
//
