LOCUS       AP028469                4261 bp    DNA     circular BCT 26-JUL-2023
DEFINITION  Serratia marcescens 12BL3 plasmid p12BL3_2 DNA, complete sequence.
ACCESSION   AP028469
VERSION     AP028469.1
DBLINK      BioProject: PRJDB10568
            BioSample: SAMD00247222
            Sequence Read Archive: DRR253313, DRR493227
KEYWORDS    .
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  AUTHORS   Nagano,D.S., Taniguchi,I., Ono,T., Katsura,K. and Hayashi,T.
  TITLE     Complete genome of Serratia marcescens 12BL3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Nagano,D.S., Taniguchi,I., Ono,T., Katsura,K. and Hayashi,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2023) Contact:Debora Satie Nagano Faculty of
            Medical Sciences, Kyushu university, Department of Bacteriology;
            3-1-1 Maidashi, Fukuoka, Fukuoka 812-8482, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8
            Genome Coverage       :: 121x
            Sequencing Technology :: MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.0 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="12BL3"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="p12BL3_2"
                     /geo_loc_name="France"
                     /collection_date="2012"
     gene            688..879
                     /gene="rop"
                     /locus_tag="SM12BL3_46390"
     CDS             688..879
                     /gene="rop"
                     /locus_tag="SM12BL3_46390"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BEL83332.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="rop"
     gene            2631..2813
                     /locus_tag="SM12BL3_46400"
     CDS             2631..2813
                     /locus_tag="SM12BL3_46400"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEL83333.1"
                     /translation="MFIFNINIYVTKSSSSCSGDGQKKKKREVGSLEVLKTPQFWGCMF
                     LIFVKLLDYISKFFN"
                     /name="hypothetical protein"
     gene            2835..3974
                     /locus_tag="SM12BL3_46410"
     CDS             2835..3974
                     /locus_tag="SM12BL3_46410"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_011104787.1"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="BEL83334.1"
                     /translation="MNKPRFNGTPIASLDSLAAMLGIDRKRLDWMVLSVSRSYKQFTVE
                     TGKNKKERKIFEPKRSLKGIQKKINKEIFENIDYPHYLHGAITGRDYISNASVHTKKKT
                     IICLDITNFYPSISKRDVTLIFKNLMRFSPDVSIALTELVTLNNQVPQGGCCSSYIANL
                     LFFNSEYNLYNRLQSIGYSYTRLLDDITISSDEDISLEEKTKVIQLVYRMVSQHRLSIN
                     EDKTTIEHTKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICQKQAAYERHTKEYH
                     DLWNRCSGKVAQMSRLGHVQANELRCVLSDILPIYDDYKIGKLKYVAKHYLSKFTHPLS
                     EEQIRKVDRMIYDFDIVGRTNEKLAKDYRSKLRALLPER"
                     /name="reverse transcriptase"
     Typing          complement(164..567)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.089"
                     /coverage="99.0"
                     /evalue="9.84e-174"
ORIGIN
        1 aacgaaaaaa ccacccggca aggtggtttt ttcgaaggtt cattaaccgg caagtttctg
       61 aaccgtggta actggctttc gcagagcaca gaaaccgaat tcgttctaac agtgtagctg
      121 tagtttacct cacacttcaa cacttctttg ttctgcgaat gcgttaccag tggctgctgc
      181 cagtggtgat aagtcgtgtc ttttcgggtt ggactcaagt tgatagttac cgaaaaggcg
      241 cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgccttc
      301 ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc
      361 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccatcagggg gaaacgcctg
      421 atatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcagt ttctgtgatg
      481 cttgtcaggg gggcggagcc tatggaaaaa cggccgctta gcggcctttc cttttcaccg
      541 tcacgcgacc gctcggtttt ctcgagcttt tatacgcatg ctcctgccgc tcgccgcagc
      601 cgaacggcag gagcatagcg actgagtgag cgaggaagcg gaaaagagaa catccctaca
      661 ctgagcactg acgctctgag gcctcttatg acgaaacaac agaagaccgc gctgaacatg
      721 gctaaattca tccagcatca gtctctgctc ctgctggaga agcttaacga gcttgatctt
      781 gatgctgagg ctgacctttg tgagaaactc cacgatgatg cggagcatct tttccgtaca
      841 ctgtcctcga gactggatga attacaggat ggtaactgat ggccagagtg tctattagtg
      901 aagcggcacg gcttgttaag gttagtcgcc ccacaattta caagatgatt aacagtggga
      961 agttaagttt cacctctgtt gtaaagcacg ggaaggctat taaagttatt gatacttcag
     1021 agcttattag agtgtttggt tctctcgatg gtgtcatcga tactgtaaaa tatgatgtaa
     1081 aaagtgacgc agaatctaca ggtgttaaca gcgttggttt acatgattta caacatagaa
     1141 ttgctttgct tgaagctgaa aacgatggtc ttaaaggggc tgttaaggct cgagatgagc
     1201 atattgattc ccttcgtcag gcaatgcagt ttctggagca caagcacgaa ccttcttcac
     1261 catccgattc cccgtggtgg aaattctgga aaaaatcctg accccgtcat gcttccgttt
     1321 tcctctccgg ccatcccggg gagttcctga accttcccaa aaatcgtcag atctctgaac
     1381 gctcctcacc actaaaaata cccacacact acgcaaaatt aacattttgc atatttgtat
     1441 ttaatatcag tgggttaact tcctggaatt tcgtttttta aattttattt atatagatcc
     1501 gactctatga cgactcgtca tagagtcccc ggcgagtcgt catagagtcg gtaacgactc
     1561 aggactataa aaacaataag ttaataacga cccgactcta gacacgactc cccgtttggt
     1621 tgttttttag acgcggcgac tctgggtacg actctagtga agagtcgccg gtgaatcgtg
     1681 tggtgagtcg tccggcgact tgccataacg tgattgccgg ccgtcgacga acccgattcc
     1741 tgaacgtgct gaaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa
     1801 attgcgtctc cgttcaccgt tttcggatgg aaagaacgcc ggaaacagga cttactctgg
     1861 ctgaatggcg tgaattttcg cgctatgcac ttgcgtgcat actcatgcat gccgtaaaaa
     1921 cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc
     1981 cgtcagaaac gccctgaggc cgttttagcg gtgcgtacaa tcatggcctt atgttaaatg
     2041 gggttgtatg ccattttcat ttaatcttag taaattcaga aagttaactt attattgagt
     2101 atttgtgagt gtaactataa aagatgatcg cttaagaact attgcgacct ttgacggcaa
     2161 gcctctcaag gatgatcgcc tgagaaccat tgcgaatgtt aacgggagtg tagggggtgt
     2221 tgtcgtggct tttgctgctc gtttattctg aagtttatac gtaatgatga ttcaggccct
     2281 ctatacgagg gattttcatc ttgttcgttt tctttgatga cagcatcata aaaaacttta
     2341 taataatcag agaagcgggc gaaccacgcc tggctctcgc tctttaaaaa atggttacgc
     2401 tttatgttgt gtcaaggaaa ccgatgcctt gttgtttaag gcgatctcac atgagatcat
     2461 gaccggcctt gccgcccatt aacggtaaga acgcccctga gcgctcatta tcgcttcaaa
     2521 aatggcgacg attgtctggc aatgtgagtt aaaagtccca aagagccaat agaatttttc
     2581 cttgctttcc tatggtctgg caaaatttca caaccactag gagcagtact atgtttattt
     2641 tcaacatcaa catttacgta accaagagct cttcatcttg ttccggagac ggacagaaga
     2701 agaaaaaaag agaggttggc tctctggaag tgctgaaaac cccgcagttt tggggttgta
     2761 tgttcttgat ttttgttaaa ttattagatt atatatcaaa attctttaac taatcgactt
     2821 ttaactcaaa catcatgaat aaacctaggt ttaatggaac acctatagca tccttggatt
     2881 ctttggctgc gatgttaggt atagacagga aaagattgga ctggatggtc ttatcagtat
     2941 ctaggtcata caagcagttt actgttgaaa caggaaaaaa taaaaaagaa agaaaaatat
     3001 ttgagcccaa gagatcttta aagggtattc aaaagaaaat aaataaagaa atatttgaaa
     3061 atatagatta ccctcattat ttgcatgggg cgataactgg gcgtgactat atatcaaatg
     3121 catccgtgca cacaaagaaa aaaacaataa tatgtctaga tataactaat ttttatccaa
     3181 gcattagtaa gcgggatgtt actcttatat ttaaaaattt aatgcgattc tctcctgatg
     3241 tatctattgc tttaactgag cttgtgacat tgaataatca agttccgcaa ggagggtgtt
     3301 gctctagtta cattgcaaat cttttgttct ttaatagtga gtataatctt tataatagat
     3361 tgcaatctat tgggtattca tacacaaggt tgttagatga tattacaata tcctctgatg
     3421 aggatatttc attagaagag aaaacaaaag taattcaact cgtctataga atggtttctc
     3481 agcataggct atccataaat gaagataaaa cgacaataga gcacaccaag gactcctctt
     3541 cgaagcttag cgtcactggg ttgtgggtga agcatggtgt tcctaaatta acaaaagaga
     3601 atagacgata tataagatat ttggtgtata tatgccaaaa gcaagctgct tatgagcggc
     3661 atacgaaaga gtatcatgat ttatggaata gatgttcagg gaaggttgca caaatgtctc
     3721 ggcttgggca tgttcaagct aatgagttaa ggtgcgttct ttctgatata ttgcctattt
     3781 atgatgacta taaaataggt aagttaaaat atgtggctaa gcattattta agtaagttta
     3841 cgcacccttt gagtgaagag caaataagaa aagttgatag aatgatctat gactttgata
     3901 tagttggtcg aactaatgaa aaactagcaa aagactatag aagtaagctt agagcgttgc
     3961 ttcctgagag gtgattatat ggaaaaaggg tttattaact tttacgacca tgttagggga
     4021 tatggtttta tacgaaggga gtgtgggcgt gacgtttttt tcaagcatga tgattttttt
     4081 gattttgaaa gtagtgttga tgtatgtaag gggattttcg ttaattttga agtaataaaa
     4141 gcagataaag ggcttagggc tgtaaatata cgaattgtat aaaatataga ggtttttgag
     4201 cctttttgat gaaatgatct cttgagatcc tacctcgctc gtcgtaatct cttgccctgt
     4261 a
//
