LOCUS       AP028327                2234 bp    DNA     circular BCT 03-APR-2024
DEFINITION  Staphylococcus epidermidis KSE124-2 plasmid pKSE124-2-5 DNA,
            complete sequence.
ACCESSION   AP028327
VERSION     AP028327.1
DBLINK      BioProject: PRJDB16027
            BioSample: SAMD00623186
            Sequence Read Archive: DRR483623, DRR486908
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1
  AUTHORS   Hisatsune,J., Komatsuzawa,H. and Sugai,M.
  TITLE     Complete nucleotide sequence of Staphylococcus epidermidis KSE124-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Hisatsune,J., Komatsuzawa,H. and Sugai,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2023) Contact:Junzo Hisatsune National Institute
            of Infectious Diseases; Aoba-cho 4-2-1, Higashimurayama 189-0002,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.5.0
            Genome Coverage       :: 100X
            Sequencing Technology :: Illumina MiSeq; oxford nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.18 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="KSE124-2"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pKSE124-2-5"
                     /geo_loc_name="Japan:Hiroshima"
                     /collection_date="2016-11-17"
     gene            284..607
                     /locus_tag="KSE1242_23430"
     CDS             284..607
                     /locus_tag="KSE1242_23430"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010730188.1"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /protein_id="BEJ47355.1"
                     /translation="MPYIYLIIAISTEVIGSAFLKSSEGFSKFIPSLGTIISFGICFYF
                     LSKTMQHLPLNITYATWAGLGLVLTTVVSIIIFKEQINLITIVSIVLIIVGVVSLNIFG
                     TSH"
                     /name="quaternary ammonium compound efflux SMR transporter
                     QacH"
     gene            complement(1200..2198)
                     /gene="repU"
                     /locus_tag="KSE1242_23440"
     CDS             complement(1200..2198)
                     /gene="repU"
                     /locus_tag="KSE1242_23440"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002322009.1"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="BEJ47356.1"
                     /translation="MQYNTTKYIDENQDNETLKDMTKSGTKRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVVKEKPKARWLFLTLSTRNAIDGEHLEQSLKHMSKAFNKLKMYTKVKKNLIGFLRST
                     EVTVNKNDGSYNQHMHVLLCVENSYFKNKTNYITQEEWVNLWQKALQVDYKPVANIKAI
                     KPNKKGDKDIQAAIKETSKYSVKSSDYLTGNHEKDAEIVQDLEQGLYRKRMLSYGGLLK
                     QKHKLLNLDDAEEGNLIQTSDEEKTTEEEQKAHSITAIWNFEKQNYFLKNL"
                     /name="repU"
     ARG             285..604
                     /name="qacC"
                     /gene="qacC"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacC"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             285..607
                     /name="qacJ"
                     /gene="qacJ"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="71.96"
                     /coverage="100.0"
     Typing          complement(1201..2198)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.906"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaaatctta atactggggt ttttttatgt caattttttc tttcaacaat aaaaacaact
       61 aaaaacactg tggtatcaat gttctattaa ctttttatat ctttgagtta tttctatata
      121 gtatcaagac aagaagaaac tcgttttcaa ctcgtttcaa aaacctttca aaaaccatca
      181 atccacaaaa ataccacgcg aatgacactc aaaatacaag actacaatta aaaaatactt
      241 agaataaaat taaataaaat acgaaaatta aaaggagtta aaaatgcctt atatttattt
      301 aataatagcc ataagtactg aagttattgg aagtgcattt cttaaatctt cagaaggctt
      361 ttcaaaattt ataccatcct taggaacaat aatttcattt ggaatttgtt tctatttttt
      421 aagtaaaaca atgcaacacc taccactaaa tataacttat gcaacttggg cgggactagg
      481 tttagtctta acaaccgtag tctcaataat tattttcaaa gaacaaataa atctaataac
      541 tatagtatct atagttttaa tcatagtcgg cgtagtttcg ttaaacattt tcggaacatc
      601 gcattaattg ctttattcca attgctttat tgacgttgag cctcggaacc cttaacaatc
      661 ccaaaacttg tcgaatggtc ggcttaatag ctcacgctat gccgacattc gtctacaagt
      721 ttagttaagg gttcttctca acatcaataa attttctcgg cataaatgct gtgttataat
      781 taaaattcct ttcttgatct atttttcact gataacacct aaatattaaa acagtgcata
      841 taagagctac caaaattttt aagggtagct ctttattttt tgttcaaaca tttattcaaa
      901 atcagaatac ctttattttt aattttaagg ggtcatttta aagaattaag ggttatttat
      961 atagttttat acctaaaaac ctaaatcggc tcttaaaacg caaatgagag ccaaataaaa
     1021 ataattgctt ttcacaaaca aaaatttgag caaaaccagt gtcgaatttt ttagacactg
     1081 cccatttttt atgcaaattt aaaaattagc ataaaaaatg ggcaaccatg ccggttgaac
     1141 gctatagttc ctgcaggggc aaaaaagctt aaaaaaacgc tagctttgat aagctaacgt
     1201 tacaaatttt ttaagaaata attttgtttt tcaaaattcc aaatagccgt aattgaatgg
     1261 gctttttgtt cttcttcggt cgttttttct tcatcgctcg tttgaattaa attaccttct
     1321 tcggcatcgt ctaaatttaa aagtttatgt ttttgtttaa gtaaaccacc gtaacttaac
     1381 atacgttttc tgtataaacc ttgttctaaa tcttgaacaa tttctgcgtc tttttcatgg
     1441 tttcctgtta aataatcgga cgacttaacc gagtattttg atgtctcttt aattgcagct
     1501 tgtatatctt tatcgccttt tttatttggc ttaattgctt tgatattcgc gacaggtttg
     1561 taatcaactt gaagagcttt ttgccataaa ttaacccatt cttcttgagt tatataatta
     1621 gttttatttt taaaatagct attttcaaca cacaataaaa catgcatatg ttgattataa
     1681 ctaccatcat ttttattaac tgttacttcg gttgaacgta aaaatccaat taaattcttt
     1741 ttaacttttg tatacatttt caatttatta aaagccttag acatatgttt taaactttgt
     1801 tctaaatgtt caccgtctat tgcatttcgt gttgatagtg taagaaataa ccaacgtgct
     1861 tttggctttt cttttacaac ttcttcaatc actttttgag cttgataact atttttcata
     1921 gcacgtctcc aattgcaaac tgggcataat ttcgatttac aaaaccaagt tttatgtaat
     1981 tttaaatacc cttcatcagt cggcttaaat tctaagacgt taccacattg ttttacgtta
     2041 aaagcctttt taatttttaa aatttctagt atatctgcat aacttacatt gtcgattttc
     2101 ttttctctcc atgggcgttt tgtcccactt ttagtcatat ctttcaatgt ttcattatct
     2161 tgattttcgt ctatatattt tgtagtatta tattgcatat aaaaagacct ccagttttga
     2221 ttttttctcg acaa
//
