LOCUS       AP028325               15527 bp    DNA     circular BCT 03-APR-2024
DEFINITION  Staphylococcus epidermidis KSE124-2 plasmid pKSE124-2-3 DNA,
            complete sequence.
ACCESSION   AP028325
VERSION     AP028325.1
DBLINK      BioProject: PRJDB16027
            BioSample: SAMD00623186
            Sequence Read Archive: DRR483623, DRR486908
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1
  AUTHORS   Hisatsune,J., Komatsuzawa,H. and Sugai,M.
  TITLE     Complete nucleotide sequence of Staphylococcus epidermidis KSE124-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15527)
  AUTHORS   Hisatsune,J., Komatsuzawa,H. and Sugai,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2023) Contact:Junzo Hisatsune National Institute
            of Infectious Diseases; Aoba-cho 4-2-1, Higashimurayama 189-0002,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.5.0
            Genome Coverage       :: 100X
            Sequencing Technology :: Illumina MiSeq; oxford nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.18 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..15527
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="KSE124-2"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pKSE124-2-3"
                     /geo_loc_name="Japan:Hiroshima"
                     /collection_date="2016-11-17"
     gene            complement(1412..2233)
                     /locus_tag="KSE1242_23260"
     CDS             complement(1412..2233)
                     /locus_tag="KSE1242_23260"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEJ47338.1"
                     /translation="MNIKDQIDISKIILQIISMLVPLVGIILSQFKGTERRMDQKKEKM
                     KRIFNNIFRFIFVIVLIDLLILVELRDFYINIKPENLIKYKKDIYQLILILTTFSTVYA
                     FFLMPFWLKKKRYYEIELEHTKGKKIAYMVLNRDKSKNKDVIIYKDDKKEYEEDVDSIK
                     QREGRVTFIPKAKSIFNLNRSAIEETKTLPIWSRWLFFILIILVSLVGIFSCIEMIMNV
                     WTFNLGKRVVMFRIIISIFPITIFMYFIAIIKFLYQSWLGKNKLVYEKSKK"
                     /name="hypothetical protein"
     gene            complement(2268..3566)
                     /locus_tag="KSE1242_23270"
     CDS             complement(2268..3566)
                     /locus_tag="KSE1242_23270"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEJ47339.1"
                     /translation="MNLEKMMDHMIGDKIRKLRKTLGLTQERFCEKYENKVSIDKYRLS
                     AIENGRREKNKNPHYLTKDQLIFFSDLMNEDITTFIYGDTQRKHQLIKLMLLNIFMNGT
                     TESGHTKDPKVEQTPFFEFNNDQEFFRLSYFNLDNNELKNMASDMNFDMHRGKEYTEQE
                     LNNRKRKILKELQKYDDFFYNEDFSRYYNLLMDGKQCFSKQSSILLKCLFGNFDFAYEF
                     LSIVESLETFRLAGLLLRQRRSSYYIDNYLRGDGSFGSKATDWKEMGYRIFITSFNEFY
                     QQHAEVFFEFFEKNIFQSRYLAITNERINSLLQSDRFLNVLNEVYENDQYIPNRMLGHN
                     FSRSMIQKLSLVEERSKRLAKKDNDLENKTIIDPTEYYDLVQVYQERIQDTYDIDRYLY
                     DLENMTLLFVDKDKDDKSAGLFLPEYFNITPLK"
                     /name="hypothetical protein"
     gene            3874..4305
                     /locus_tag="KSE1242_23280"
     CDS             3874..4305
                     /locus_tag="KSE1242_23280"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011837674.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase sigma-70 factor"
                     /protein_id="BEJ47340.1"
                     /translation="MNQVKSKREANIQIAYKNGDWDTISKLLNQSYENARRKDRYYQVL
                     SLNYPNINSQGQISELENFIASSTLTPIECLLLKERQIEVNKALSLLSKTDYEMIIGFY
                     LDHKSYSQISREVGISDKTVKKRIIKATTFLKQSLENIT"
                     /name="DNA-directed RNA polymerase sigma-70 factor"
     gene            4387..4707
                     /locus_tag="KSE1242_23290"
     CDS             4387..4707
                     /locus_tag="KSE1242_23290"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_003549061.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEJ47341.1"
                     /translation="MKITQKFNKGGYSAEIASNYIDNKKPIYSLSNELYPQQRFENNQP
                     TGEIIAYKAWFIQEGLPPFMVKFESEVTLPNFMTVITFENLEACEIRYDVYFKASDIKE
                     VK"
                     /name="hypothetical protein"
     gene            4707..5783
                     /locus_tag="KSE1242_23300"
     CDS             4707..5783
                     /locus_tag="KSE1242_23300"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011837675.1"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="BEJ47342.1"
                     /translation="MTSKDLEKYLLQSLNMALGSIMQGETSYTNSFSCKVTEEGFYFLP
                     RLPAGYIIDDELYQKIFLIASAALYPKFTLLKQSSAYFVPMDTNDIHTQRALFFPWQQG
                     ISERLVITDLDRFTSTLTNQHIPIMKQLSLNYQKITSIAIAGNSGSGKSYMLTYLLSVL
                     EHISDLIIVDPKFDTPSRWARIHDINVIHPHQNRSKSDFLSEVNENLSHCLQIIQRRQT
                     TLYDDPDYQFEHLTIVIDEVLALAEGVNKAIKDAFFSLLSQIALLGRATKVHLLLVSQR
                     FDHQTIPISVREQLNVLIQIGNINKKTTQFLFPDLDPEGIVIPIGKGTGLIQVIDSESP
                     YQVLPLLCPTYYTKEGIL"
                     /name="cell division protein FtsK"
     gene            5783..6385
                     /locus_tag="KSE1242_23310"
     CDS             5783..6385
                     /locus_tag="KSE1242_23310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_006268963.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEJ47343.1"
                     /translation="MRKTYFMRVYNITFYLFIWAILTFLFSQVFHLLKNTLAQSDDIYI
                     SKITPYVTFISKGLYHITFYFLDIALLLVGIDLVIRLLKDKITNHIKSVYHTIILRRYI
                     KIDVEKVPSKDNQSKDEAKNSAVQQYFNKVVGKSLVDVRQKEVFVAIKIPRKHQAHKIL
                     NDIENQIYKEITYQHPDYYFSTPERQNRYLWFKGQRR"
                     /name="hypothetical protein"
     gene            6516..7085
                     /locus_tag="KSE1242_23320"
     CDS             6516..7085
                     /locus_tag="KSE1242_23320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002919940.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="BEJ47344.1"
                     /translation="MKKDKRSNKWAFLIYEESTPSDYRDILEQMHIPYILSPWHNQDID
                     YSTGSIKKSHKHGALFFDSLKSYSQVSELLQQNLNSPSHVEIIMSPKGMFDYFIHASNP
                     DKTPYDINDIETGCGFDLNQFLLEQGQSEIFNAIIDLIESQDFTEFQNLVIYARQHHPS
                     YLNLIIQRTYFFAKYLDSRRHTSNHQ"
                     /name="plasmid replication protein"
     gene            complement(7076..8026)
                     /locus_tag="KSE1242_23330"
     CDS             complement(7076..8026)
                     /locus_tag="KSE1242_23330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117516.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="BEJ47345.1"
                     /translation="MSNFKRIAIQEFEKERFYKLYKFLFEDDYFKKLSDSSKIAYCMFR
                     DRFELSKMNNWIDESGNVYLIFTTKDLCNLLNCGTQKVTKIKKELENFNLLEQERMGLN
                     KPNKIYILEPQTPFKYNSNKEFRKSKFQSFENQNSRVLKVKKQEFRKSKSNDTDSSDTD
                     YIETKNNDTNDTNDTSKMISNNSHSNHTNHQQTEFNNEALKFQVLEELPSQIKSYLSNF
                     EIKDIRIIKSVLLKGKKSFNNAHDTYYRLEDVEFELVSVLKRFKAMLLQKDESIEAMQG
                     YLMQSIKAELEEMHALNMRRQNAPQKNIFHTSNHW"
                     /name="replication protein"
     gene            8454..9149
                     /locus_tag="KSE1242_23340"
     CDS             8454..9149
                     /locus_tag="KSE1242_23340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000843735.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BEJ47346.1"
                     /translation="MKSVKILSEELGVSKQTIFNNIKRLNIETIKQENTSFIKENTDIE
                     KIIQRVNENKKKYGFESTTEEKQKKESDNSNIKSKSDTQIIEILKKQIDTLNEQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQILALESNKKIQKLEHQLEEERQLNYSFDKSTNDR
                     ENVDVQEASYTSDSVNTDQYQKEEKKPEVQPKDISENQQDDSFNQNDKDIAIEETQTKK
                     GFWSRLFGG"
                     /name="DUF536 domain-containing protein"
     gene            complement(9231..9905)
                     /locus_tag="KSE1242_23350"
     CDS             complement(9231..9905)
                     /locus_tag="KSE1242_23350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001105984.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="BEJ47347.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYDKWRIDETYIKIKGKWNYLYRAIDAVEHTLDIWL
                     RKQRDNHSAYVFIKHLIKQLDKPQKVITDQAPSTKVAMTKVIKAFKLKPDCHCTSQYLN
                     NLIEQDHRHIRVRKTRYQSINTTKNTLKGIECIYALYKKNRRFLQIYGFSPYHEIRHML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(9930..10199)
                     /locus_tag="KSE1242_23360"
     CDS             complement(9930..10199)
                     /locus_tag="KSE1242_23360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001831938.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BEJ47348.1"
                     /translation="MYKDYNMTQLTLPLETSVLIPTNDISRHVNDIVETIPKTEFDEFR
                     HHRGATSYHPKMMLKVILYAYTQSVFSGRKIEKLRFCCKVEFII"
                     /name="transposase"
     gene            complement(10572..10943)
                     /locus_tag="KSE1242_23370"
     CDS             complement(10572..10943)
                     /locus_tag="KSE1242_23370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEJ47349.1"
                     /translation="MITDAQKKYLKIIFQLGGDIELVSNKMLSKKLGAALPSISQMLDR
                     LTEAGFVKSEHYRGVMLTKKGLTYTIEMTNNHEIIKAFFIKVLNINSSEVNQEVDLIEN
                     HVSDKFVQRLSEYLRTKQF"
                     /name="hypothetical protein"
     gene            11074..11811
                     /gene="mtsB"
                     /locus_tag="KSE1242_23380"
     CDS             11074..11811
                     /gene="mtsB"
                     /locus_tag="KSE1242_23380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_003130243.1"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese ABC transporter ATP-binding protein"
                     /protein_id="BEJ47350.1"
                     /translation="MLDVQNLSVVYDNQTRALQDVSLQMIEGQIVGIIGPNGAGKSTLI
                     KAMINLIPHKGQTFFKSKPIHKQLKNLSYVEQKSNIDTTFPMKVLDCVILGIYPRLKPW
                     QLPGKKEKAMATEALNKVDMSEYKDRQIGELSGGQFQRVLIARTLVQKTDLILLDEPFV
                     GIDITSENIIVDLLKELAESGKTILIVHHDLSKVSKYFDSLILLNKHLIDFGSVENVFT
                     GDNLAQAFGTNILIPSESGDKND"
                     /name="mtsB"
     gene            11804..12643
                     /gene="mntC"
                     /locus_tag="KSE1242_23390"
     CDS             11804..12643
                     /gene="mntC"
                     /locus_tag="KSE1242_23390"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:Q8Y652"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese transport system membrane protein MntC"
                     /protein_id="BEJ47351.1"
                     /translation="MISQFIHELFQFSFLQRALVTSVVIGIICGVIGCFIVLRSLALMG
                     DAISHAVLPGVAISYMMGINFFYGAVVFGIMAALGIGFVNQNSKIKSDSSIGIVFSAFL
                     ALGVLLVTKAQSSIDLTEILFGNVLTVKEQDRTLTFIVAAIVLIIVVIFYKEFLLSTFD
                     PTMAAASGLPTKFIHYTIMVLLTLVTVASLQTVGVILVVSLLITPASTAYLLTKRLSTM
                     ILLSALIGAISSIIGLFFSFTFNLPSGVVIVLVITAIFIITFFLAPRNGLLWRMIKH"
                     /name="mntC"
     gene            12662..13615
                     /gene="mntA"
                     /locus_tag="KSE1242_23400"
     CDS             12662..13615
                     /gene="mntA"
                     /locus_tag="KSE1242_23400"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:Q8Y653"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding lipoprotein MntA"
                     /protein_id="BEJ47352.1"
                     /translation="MKKVILFLFTITLSVVLAACSNGDKDGDSDKELESVDKDKIKVVT
                     SFSMIDDMVKEIGGEHVEVKNLVPTGTDPHDYDPKPDDIKHISSADLVFYNGLNLEGGS
                     KGWLFKALESSDFSKENAIKTSEGVKPKYIKDEDGKKEVNPHAFIDPKVGEKMIKNITK
                     SLSERNPKNKDYYKENEKAYLKKLHNIEDDYEKQLGDIPKKDRVFVASEQAFQYLTDRY
                     DLKEGYIWAIDTDENGSPEQIKDLVKFIDKNEPSHLFVESNVDKRPMETVSKESGVSIY
                     KKPIYSDEISKEGGVADTYLKYLEYNLDVLTDGLEK"
                     /name="mntA"
     gene            complement(13858..14463)
                     /locus_tag="KSE1242_23410"
     CDS             complement(13858..14463)
                     /locus_tag="KSE1242_23410"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000635275.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BEJ47353.1"
                     /translation="MIVGYARVSTIDQNLDRQIENLEAFGAEKIFTEKQSGKSIEDRPI
                     FIETLNFVRMGDRLIVESIDRLGRNYDEIIQTVNYLKEKEVQLMITSLPMMNEAIGNPL
                     LDKFMKDLIIQILAMISEQERNESKRRQAQGIKVAKEKGIYKGRPVLYSPNAKDPQKRL
                     VYQRVVEMLDQGISISKIAKEVDITRQTVYRIKNERTK"
                     /name="transposase"
     Typing          complement(7120..8026)
                     /name="rep_cluster_1216"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.706"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          8456..9056
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.281"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 aaattcgaca gaagttaata aggctattga aacaacttta aaaaacaaaa aaagttccaa
       61 tgatttttgg tggcttaata atggtattac tatactagct gatgaaggat cattaaatgg
      121 tagtgtgttt acactagaaa atatccaaat agttaacggc ctacaaactt cttactcaat
      181 atttaatgtt ttaagtgaag aaaagaacaa tgagaatgaa gatagatctg ttttttgtaa
      241 aataataata acgcaagaag aagagagtat agattccatt ataaaagcta caaattctca
      301 aaattcaata cctgcttcat cacttcgatc tacagataac ttacaacgag acattgaatt
      361 atatcttttc aaaaaagatt tcttttatga tagaagaaaa aatttctata aaaacaaaaa
      421 gaaaccaaga aataaaatta ttagtataaa ctacctggct caatctctaa cttcaatatt
      481 ggaaatgaag ccctctaaag ctcgtactag tcccacagtc cttacaaaat ctgatgagga
      541 ctatgagaaa attttcaacc gtaatatgtc tattgaaatt tactactatg ctattgtttt
      601 aagaaaaaat gtagagactt atttaaaaga gaattttaat atcaaaacag atatagataa
      661 ggatattaag aactactttt atttacattt attaagaatt acatccagtt tactgatatc
      721 tgataaagcc attacttata ataaaatact taatttagaa gaaggtaaaa ttctagaaat
      781 cgataattct tcaatagaag aagctatcaa cattttaaaa gaaataatat acaccgaaaa
      841 agataaagac agtaatgtta atctaaccaa tttttcaaaa agtgagaaat taaatgattt
      901 aatcaatcaa aaacttgcag attactttat agataaataa cctaatatat attgactgat
      961 ccaagtaact ttataaaaat cacttttcat aagtgaattt tgcattccta ctcactatgg
     1021 tgttataata tatttaacaa atattagact catgaatcac cgaaccccca atctatggct
     1081 gtagattggg ggttcttttg atggtgaggc tttgtcgcct tattatttat tgcgattgtt
     1141 tagccaaaac gtaaaaatcg caactataca ccctgatatt atgggtgcaa ttatgctaac
     1201 aaatattaaa ctcatgattt cacctccttc cgccgaaaat accgccggaa gttaaggcga
     1261 cgccattatt ataccatctg tgaatataca agcatatcta cttccgataa gtgaaattat
     1321 gtttttaaat atctattggc ccgacttaat ttcagtctta cgattttaga gatacatttt
     1381 tctattaaag tctattaatg atttatcttt attatttttt gctcttctca tatactaatt
     1441 tattttttcc taaccaagat tgatataaaa acttaattat ggcaataaaa tacatgaaaa
     1501 tagtaattgg gaatatagat ataattattc taaacattac tactcttttt cctaaattaa
     1561 aagtccagac attcattatc atttcaatgc aactgaaaat cccaactaaa gaaactaata
     1621 ttatcaatat aaaaaataac catctgctcc atataggtaa tgttttagtt tcttctatag
     1681 ccgacctatt aagattaaat atagattttg ctttcggaat aaatgtaact cgtccctctc
     1741 tctgcttaat tgaatcaaca tcttcttcat attctttttt atcatcttta tatattatta
     1801 catccttatt ttttgattta tctctattca ataccatata agctattttt ttcccttttg
     1861 tgtgttccaa ttctatttca taatatctct tcttttttag ccaaaatggc attaagaaaa
     1921 aagcatatac tgtagaaaaa gttgttaata ttaaaattaa ttgataaata tcttttttat
     1981 atttaatcag attttcaggc ttaatattta tataaaagtc tctaagttcc actaatataa
     2041 gtaaatctat caatactatt acaaatataa acctaaatat attattaaat atgcgtttca
     2101 ttttttcttt cttttggtcc attcttcttt cagtcccttt aaattggctc agtataatac
     2161 caactaacgg aacaagcata ctaatgattt gtaaaataat tttactgatg tctatttgat
     2221 ctttaatatt catttctatt tatcctttta ttatctttga aataaattta tttcaaagga
     2281 gtaatattaa agtactctgg taaaaacaac cctgcactct tatcatcttt atctttatca
     2341 acaaataata aagtcatatt ttctaaatca tataaatacc tatctatatc ataagtatct
     2401 tgtattcttt cctgataaac ttgaactaaa tcatagtatt ctgttggatc aatgattgtt
     2461 ttattttcta aatcgttatc ttttttagcc aaccttttcg accgttcttc taccaatgat
     2521 aacttttgaa tcattgacct tgaaaaatta tgccctaaca ttctattagg tatgtattga
     2581 tcattttcat agacctcatt taatacattt aaaaatctat cactttggag tagtgaattg
     2641 atgcgttcat ttgttatagc aagataacgt gattgaaaaa tatttttttc aaagaactca
     2701 aagaatactt cagcatgttg ttgataaaat tcattgaagc tagttataaa tattctataa
     2761 cccatttcct tccaatctgt cgctttagat ccaaaacttc catcgcctct aagataatta
     2821 tcaatataat agcttgatct tcgttgtcta agtagaaggc ctgctaaacg gaatgtctcc
     2881 aaactttcta caattgacaa aaattcataa gcaaagtcaa aatttccaaa taagcatttt
     2941 aatagtatag aagattgctt cgaaaaacac tgttttccat ccattaataa attataatat
     3001 cgagaaaaat cttcattata aaagaaatca tcgtattttt gtaattcttt caatattttt
     3061 ctttttctat tatttaactc ttgttcagta tattcttttc ccctatgcat atcaaagttc
     3121 atatcagacg ccatattctt caactcatta ttatctaaat taaaataaga tagtctaaaa
     3181 aattcttggt cattgttaaa ctcaaagaat ggtgtttgct ctactttagg atccttagta
     3241 tgacctgatt cagtagttcc attcataaaa atatttaata acattaattt tattaattga
     3301 tgctttcttt gagtatcccc ataaataaat gttgtaatat cttcattcat taaatcagaa
     3361 aaaaatataa gttgatcttt agtaagataa tgaggatttt tatttttttc tcttctgcca
     3421 ttttctattg ctgatagtct atatttatca atacttactt tattttcata cttttcgcaa
     3481 aatctctctt gtgttaaacc tagtgtcttt cttaactttc ttattttatc cccgatcata
     3541 tgatccatca ttttttctaa attcattacg aaacctccat atattggtaa ttgagtaaca
     3601 ttttcgaaaa gttggtagat tttcaagata aaaagcgaca acttttacta actacttcaa
     3661 tcgtattttt tgagaagata taactaaatc aaagtgatct ttgattacac agtcctatag
     3721 atgcgcatca aacggacgga ttgaaacgat taagctttta ttatttatat aaaagtttga
     3781 tataacatgt ttcaccgttg atttggtgtg cttttttata tcaaaaatgt cgtttctttc
     3841 tatatttaca catattgaag gagtgactga tatatgaacc aagtaaaaag taaaagagaa
     3901 gctaatatac aaatcgctta taagaatgga gattgggata ctatatctaa attactcaat
     3961 caatcttatg agaatgctcg acgtaaagat agatactatc aagtcctttc tttaaattat
     4021 cctaatataa actcacaagg tcaaatatca gaacttgaaa actttatcgc tagttccaca
     4081 ctaacaccta tagaatgtct tttattaaaa gaacgtcaaa ttgaagtaaa taaagcactg
     4141 tctctattaa gtaaaaccga ttatgagatg attataggct tttatttaga tcataaaagt
     4201 tattcacaaa tcagtagaga agttggtatc agtgacaaaa ctgtaaaaaa gcgaattatt
     4261 aaagcaacaa cgtttcttaa gcaatctcta gaaaatatta cttaatagat atgctttccg
     4321 agatactttt aatttttctc ctaataagta ggaattaatt taagaaggaa ataaggagtg
     4381 cattatatga agattacaca gaaatttaat aaaggtgggt attcagctga aatagcttca
     4441 aactatatcg acaacaagaa acccatttat agcttaagta atgaacttta tccgcaacaa
     4501 cgttttgaaa ataatcaacc aactggagaa atcattgcgt ataaagcttg gtttattcaa
     4561 gaaggattgc cacctttcat ggtcaagttt gaaagtgaag tgacattgcc gaattttatg
     4621 acggtcatta ctttcgaaaa cttagaagct tgcgaaattc gttatgatgt ttactttaaa
     4681 gcatcagata ttaaggaggt taaataatga catcaaaaga tctagaaaag tatctccttc
     4741 aaagtttaaa tatggcactc ggatctatta tgcaaggaga gacaagttat accaatagtt
     4801 ttagctgcaa ggttacggaa gaaggttttt actttcttcc tcgcctccct gcaggctaca
     4861 ttattgacga tgagttatat caaaaaatat ttcttatcgc aagtgcagct ttatacccca
     4921 agtttacact gctaaaacaa agctctgcat atttcgttcc tatggatact aatgatattc
     4981 acacacaacg tgcgctattt tttccatggc aacaagggat atctgagcgc ttagtaatta
     5041 cggacttaga tcggttcact tctacgctaa cgaaccaaca cattccaatc atgaaacaat
     5101 tatccttaaa ctatcagaag attacatcca tcgcaatcgc aggcaattca ggcagtggta
     5161 aatcttatat gttgacctat ttattaagtg tattggaaca catatctgat ctcattattg
     5221 ttgatcccaa gtttgatact cccagtcgat gggcacgtat tcatgatatt aatgtgattc
     5281 atcctcatca aaatcgatct aaatctgatt ttttatctga ggtgaatgaa aacttgagtc
     5341 attgtcttca aatcattcaa cggcgtcaaa caactctgta cgatgatcca gattatcaat
     5401 ttgaacactt aaccattgtg attgatgaag tcctcgcttt ggccgaaggt gtgaataaag
     5461 ctattaaaga tgctttcttt tcattactat cacaaattgc tttattaggt cgtgcaacca
     5521 aagtacattt attgctggta tctcaacggt ttgatcatca aaccataccg atttcggtaa
     5581 gagaacaact taatgttctc attcaaatcg gtaatatcaa taaaaagaca acgcaatttc
     5641 tgtttcccga tttggatccc gaaggtattg tgatacctat tgggaaagga acggggctga
     5701 ttcaagtcat tgatagcgag tccccctatc aagtcttgcc attattatgt cctacttact
     5761 atacgaaaga aggtatctta tgatgaggaa aacatatttt atgcgtgtgt ataacatcac
     5821 tttttattta ttcatttggg ccattttaac tttcctcttc agtcaggtct tccatctttt
     5881 aaaaaacact ttagcacaaa gcgatgatat ctatataagt aaaataacgc cctatgttac
     5941 attcatttca aagggcttat atcatatcac tttttacttt cttgatatcg ctctattgct
     6001 tgttggtatt gatttagtga ttcgtttatt aaaagataaa ataacgaatc atattaagtc
     6061 tgtctatcat actattatat tgcgacgtta tatcaaaata gatgttgaaa aagtcccgtc
     6121 taaggataat caatcaaaag atgaagcaaa aaatagtgct gtacaacaat attttaacaa
     6181 agtggtaggt aaatcacttg ttgatgttag acaaaaggaa gtttttgttg ccattaaaat
     6241 acctcgaaaa catcaagctc ataaaattct caatgatatt gaaaatcaaa tttataaaga
     6301 aatcacttat cagcatcccg attattactt ttcaacacct gaacgtcaga atcgttattt
     6361 atggtttaaa ggacaaagac gatgatggat gtcaaagggc gttgtgtttc acgtagtgaa
     6421 aggcacaaac gccctttgac aatttgtaag atggcatggg gttactacga cccccatgcc
     6481 atcattaatc aataatcaca gaaaggagat cccctatgaa aaaagataaa cgttcaaata
     6541 aatgggcatt cctaatttat gaagaaagca caccctctga ttatcgagat attcttgaac
     6601 aaatgcatat tccttatatt ttgagcccat ggcacaatca agatatagat tattcaacag
     6661 gatctatcaa gaagtcgcat aaacatgggg ctttattctt tgattcatta aaaagttatt
     6721 ctcaagtctc tgaattactt caacaaaact taaattcacc cagtcacgtt gaaattatta
     6781 tgtctcctaa agggatgttc gactatttta ttcatgcctc taatccagat aaaactcctt
     6841 atgatatcaa cgatattgaa actggttgtg ggtttgattt aaatcaattt ttacttgagc
     6901 aaggacaatc tgaaattttt aatgccatta ttgatttgat tgaaagccaa gactttacag
     6961 aatttcaaaa cttagtgata tatgcgcgac aacatcatcc atcgtattta aatctaatta
     7021 tccaacgaac ttactttttc gcaaaatatt tagattctag acgccataca tcaaatcacc
     7081 aatgatttga tgtatgaaat atattttttt gtggcgcatt ttgacgacgc atatttaagg
     7141 catgcatttc ttcaagttca gctttaattg attgcattaa atagccttgc atggcttcaa
     7201 tgctttcatc tttttgtagc aacatggctt taaaacgttt taaaacactc acaagttcaa
     7261 attcgacgtc ttctaaacgg taatatgtat catgtgcatt attaaatgat ttttttcctt
     7321 tgagtaaaac acttttaata atacggatgt ctttaatttc aaaattactt aaataagatt
     7381 tgatttgcga aggtaattct tcaagcacct ggaattttaa ggcctcatta ttaaattcgg
     7441 tttgttgatg atttgtatga ttcgaatgag aattgttaga aatcatttta gatgtatcat
     7501 tcgtatcatt cgtatcatta tttttagtct ctatataatc agtatcactt gaatcagtat
     7561 cattagattt tgattttcga aattcttgtt ttttgacttt caaaactctg gaattttgat
     7621 tttcaaaact ctggaatttt gattttcgaa attccttgtt actattgtat ttaaaaggtg
     7681 tttggggttc taatatatat atcttgtttg gtttgtttaa ccccattctt tcttgttcta
     7741 ataagttaaa gttttctaat tcttttttaa ttttagtaac tttttgagtt ccgcaattta
     7801 aaagattaca taaatctttg gtagtaaata ttaaatatac atttccactt tcatcaatcc
     7861 aattattcat tttagataat tcaaaacgat ctctaaacat acagtaagca atttttgaag
     7921 aatcactaag ttttttaaaa taatcatctt caaataaaaa tttataaagt ttataaaatc
     7981 tttctttttc aaattcttgt attgcaattc ttttgaaatt tgacataata aaaacctctt
     8041 tccgaaattt agtttggtaa gaggtaataa aacgctttta tatagcgtta catttattta
     8101 atgatttaag tgcttgcgct aaggtgttat tcattatata atacaagtat aaatttgata
     8161 ttaaacaaac gtgctgcaac gttttattac cgtaccccga ctattaccag tagttggggt
     8221 ttttaatgtg ttaagactat tatagcactt taaataagct tataaaagaa gaaaataata
     8281 ctttgtcaaa ggttggtaaa gtgttgatga actttgctaa tcgtcatcaa cctttgacaa
     8341 agtgttgatg aaaatttaat tttctcatct ctaactaata atggctttga ttataaaact
     8401 atataagttt ataatgtaaa gggtttataa acattttata aagggttgat aaagtgaaaa
     8461 gtgtaaaaat cttaagtgaa gaattagggg tcagtaagca aacgattttt aataacatta
     8521 agcgattaaa tatagagaca ataaaacagg aaaatacttc atttattaaa gaaaataccg
     8581 atatagaaaa aatcattcaa agagtcaatg aaaacaaaaa gaaatatggt tttgaaagta
     8641 ctacagaaga aaagcaaaaa aaagaatcag ataatagtaa tattaaatcg aaaagtgata
     8701 ctcaaataat agaaattcta aaaaaacaga ttgatacttt aaatgaacaa attgaaaaac
     8761 aagaaagtcg tcatgaaacc acaattgaat tctatagaaa agagttgcag gaaaggtcaa
     8821 aattacttga aaaccaacaa attttagcct tagaaagcaa taaaaagatt caaaaattgg
     8881 aacatcaatt agaggaagag agacaattaa attattcctt tgataaatct actaatgatc
     8941 gagaaaacgt tgatgtacaa gaagcatcat atacttcgga ttctgtaaat actgatcaat
     9001 atcagaaaga agaaaagaaa ccagaggttc aacctaaaga tatatctgag aatcaacaag
     9061 atgattcatt taatcaaaat gataaagata ttgctataga agaaacccaa actaaaaaag
     9121 gcttctggag tcgtttgttt ggtggctaat gattgtttga aaaaatatat ggttctgttg
     9181 caaagttaga aaaatatagc taattaccat tttatcatgt cgttgttccc ttaactggct
     9241 aacatatgcc taatttcgtg gtatggcgaa aatccgtaga tctgaagaaa cctgcggttc
     9301 tttttatata gagcgtaaat acattcaata ccttttaaag tattctttgt cgtattgata
     9361 ctttgatatc ttgtctttct tactctaata tgacggtgat cttgctcaat gagattattc
     9421 agatattgcg atgtacaatg acagtcaggt ttaagtttaa aagctttaat gactttagtc
     9481 attgcgacct tcgttgaagg tgcctgatct gtaattacct tttgaggttt atccaattgt
     9541 ttaatgagat gtttgataaa tacatatgct gaatgattat ctcgttgctt acgcaaccaa
     9601 atatctaatg tatgttccac tgcatcaatg gcacgatata aatagttcca ttttcctttt
     9661 attttgatgt acgtctcatc aatacgccat ttgtcataag cttttttatg ctttttcttc
     9721 caaatttgat ataaaatcgg ggcatattct tgaacccaac ggtagaccgt tgaatgatga
     9781 acgtttacac cacgttccct taatatttca gatatatcac gataactcaa tgcatatctt
     9841 agatagtagc caacggctac agtgataaca tccttgttga attgtttata tctgaaatag
     9901 ttcatacaga agactccttt ttgttaaaat tatattataa attcaacttt gcaacagaac
     9961 cgtaattttt ctatcttacg tcctgaaaac acagattgtg tgtaggcata tagaatgact
    10021 tttaacatca ttttgggatg gtatgaggtt gcaccacgat gatgtctgaa ttcatcgaat
    10081 tcagttttag gaattgtttc tacaatatca ttgacatgtc gtgaaatatc atttgtgggg
    10141 ataagaactg aagtttccaa tggtagagta agttgagtca tgttataatc tttatacata
    10201 aggcacctcg ttaatttagt ttagtgacat ttattaaatt atacgaaagt gccttatttt
    10261 tttaaagtat ttcaatgtaa aattacatat aaatacaaag tattttggcg agactcttga
    10321 gggaacagga caagttgaag actacaggct gaagctgtcc cctaagaaaa cgagccaaca
    10381 atacgtagta ttgtaaataa agaagccagt aaatgaattt ataataaact catttactgg
    10441 ctaatttgtt agaaattatg tcccagcctc atttatttat gattcgtatt cttattttga
    10501 gggctgggag agaataattg ttgctttgta ctgtactttt tattaaaaag cttcatagtt
    10561 aatcatcctt tttaaaattg ttttgttctt aagtattcag ataaacgttg aacaaattta
    10621 tctgatacgt gattttcgat taaatcaacc tcttggttga cttcagaaga gttgatattt
    10681 aatactttta tgaaaaacgc tttaataatc tcatgattgt tagtcatttc tatcgtataa
    10741 gttaatcctt tcttagttaa cataacacca cgataatgtt cacttttgac aaaacctgct
    10801 tcggttaatc gatctaacat ttggctaatt gaagggagcg ctgctcctaa ctttttagat
    10861 aacattttat tagacactag ctcaatgtct ccacctaatt ggaagataat tttcaaatat
    10921 ttcttttgcg catcagtaat catgatccca cctacaattt tgatttttaa agcagaaata
    10981 gctagccaaa cttcaaattt tcgtttgact aaataatata tttaggataa cctaaatata
    11041 ttatttacgc aagtgaagaa agaagggaat agcgtgttag atgtacaaaa cttgtcagtg
    11101 gtttatgata atcaaacccg agctttacaa gatgtttctt tacaaatgat agaaggacaa
    11161 attgttggga ttattggccc aaatggggca ggtaaatcaa ctttgattaa agcaatgatt
    11221 aatttaatac cacacaaagg acaaacattt tttaaaagta agcctataca taagcaactt
    11281 aaaaatcttt catacgtcga acaaaaaagt aatatcgata caacgtttcc tatgaaagtt
    11341 ttagattgtg tcattttagg tatataccct cggttaaaac cgtggcagtt acctgggaaa
    11401 aaagaaaaag caatggcaac agaagccctt aacaaagtgg atatgtcgga atataaagat
    11461 cgacaaatag gggagctatc aggaggacaa tttcaacgag tgttaattgc tagaacgtta
    11521 gttcagaaaa cagatttgat attacttgat gaaccttttg ttggcatcga tattacaagt
    11581 gaaaatatca ttgttgattt attaaaagaa ctagctgaat ctggaaaaac aattttaatt
    11641 gttcatcatg acttatccaa agtcagtaaa tatttcgata gccttattct tttaaataaa
    11701 catttaatcg attttggttc tgttgaaaat gtgtttacgg gagataactt agcacaagcc
    11761 tttggcacaa atattttgat cccttctgaa agtggggata aaaatgatta gtcaatttat
    11821 acatgagtta tttcagttta gttttcttca aagagcactt gtaacttctg ttgtcatcgg
    11881 aatcatctgt ggcgttattg gttgtttcat cgtattaaga agtttggcgc taatgggaga
    11941 tgctatctca catgcagttc taccgggtgt agctatttct tatatgatgg gcattaattt
    12001 tttctacggt gcggtagtct ttgggattat ggcagcttta ggaatcggtt tcgttaacca
    12061 aaacagtaaa atcaaaagcg attcatctat aggtattgtt ttttctgcct ttctagcttt
    12121 aggggtttta cttgttacga aagcgcaatc gtccatagat ttaacggaaa ttttatttgg
    12181 taatgtactg acagttaaag aacaggatcg aacattaaca tttattgtcg ctgcgattgt
    12241 cttaattatt gtagtgatct tttataaaga atttttacta tcaacgtttg atccaaccat
    12301 ggctgcagct tcaggtttac caacaaaatt catccattac acaatcatgg tgttattaac
    12361 gttagtaact gttgcctcat tacaaaccgt cggtgttatt ttagttgttt ctttattgat
    12421 tacgccagct tcgacagctt atttactgac taaaagacta tcaaccatga ttcttttatc
    12481 agcgttgata ggtgctattt catctattat tgggttgttc tttagtttta catttaactt
    12541 accttcaggt gtggttattg tattagttat tacagctata tttattatca cattcttctt
    12601 agcacctaga aatgggctat tgtggagaat gattaaacat taacttagga ggatttttat
    12661 aatgaaaaaa gtgatattat ttttattcac cattacactt tctgttgttt tagcagcttg
    12721 tagtaatgga gataaagacg gtgattcaga taaagaatta gaatcagtag ataaagataa
    12781 aatcaaagtg gtcacaagtt tttctatgat tgatgatatg gttaaagaaa ttggcggaga
    12841 gcacgttgaa gttaaaaatt tagtgcctac aggcactgac ccgcatgact acgatcctaa
    12901 accagatgac attaaacata tctcgagtgc cgatttggtt ttctataatg gattgaattt
    12961 agaaggtggc agtaaaggtt ggttatttaa agctttagag tcttctgatt tttcaaaaga
    13021 aaatgcgata aaaacatcag aaggtgttaa acctaaatac attaaagatg aagatggcaa
    13081 aaaagaagtg aaccctcatg catttattga tccgaaagta ggagaaaaaa tgattaaaaa
    13141 tatcacgaaa tctttatctg aaagaaatcc aaaaaacaaa gattattata aagaaaatga
    13201 aaaagcatat ttaaaaaaat tacataacat cgaagatgat tatgaaaaac aattaggcga
    13261 tattcctaag aaagatcgtg tttttgtagc gagtgaacaa gctttccaat atttaacaga
    13321 ccgttatgac ctaaaagaag gttatatttg ggcgatagac acggacgaga atggatctcc
    13381 agaacaaatc aaagatttag taaaatttat tgataaaaat gagccatctc acttatttgt
    13441 agaatcaaac gtagataagc gtccaatgga aaccgtatct aaagaaagtg gcgtatcaat
    13501 ttacaagaaa ccaatttact ctgatgaaat tagtaaagaa ggtggcgtag ctgatactta
    13561 tcttaaatat ttagagtata atttagatgt tttaactgat ggattggaaa aataacaatg
    13621 aaactaaaag tgagcgttat tgtctatata gataataacg ctcacttttt tacttatctt
    13681 ttggaagaga tgtttcgtct tcatatctta ttaaagggaa aaatatgaag aagtttgaaa
    13741 ttatcatttt tacagtttaa taaagtttag aattttaaat taaaagaagc aggttattgt
    13801 ataacatatt atcatttcct aatgtgttat acaatcactt tttactcatt ttattaatta
    13861 ttttgttctt tcatttttaa ttcgatatac ggtctgtctt gtaatatcta cttctttagc
    13921 aattttacta atagaaattc cttgatctaa catttctaca acacgttgat aaactaaacg
    13981 tttctgtggg tctttcgcgt ttggtgagta aaggacaggt ctgcctttat agataccttt
    14041 ttctttcgct accttaattc cctgtgcttg cctacgctta ctttcatttc tttcttgttc
    14101 tgaaatcatt gctaaaattt gaatgattaa atctttcatg aacttatcta ataatgggtt
    14161 accaattgct tcattcatca taggcaagct tgtaatcatt aattgaactt ctttttcttt
    14221 taaatagttc accgtttgaa taatctcgtc ataatttctt cctagacggt caatcgattc
    14281 aacgattaaa cgatctccca ttcttacaaa gttcaatgtt tctataaaaa taggtctatc
    14341 ttcaatcgat ttacctgatt gtttttctgt gaatattttt tcagcaccaa atgcttctaa
    14401 attttcaatc tgtctatcta aattctgatc aatggttgat actcgtgcat aaccaactat
    14461 cattttcaac accctttctc atacacccta atcatacatt tagaaactgt attttacaag
    14521 ggctcctaat tgttcaatta gggtagactc ttatcatgcg aatttcataa tcaaagaaat
    14581 aatgaagtta aatggcctga ttgattgttt ataatgtata ataaacacaa taagtttttt
    14641 acaattaaaa taggtgggat taatatggca aataatagtg cgctacttga agcaataata
    14701 tcagattatt caaaagaaaa ttttccaaat gaaaataatt cagatcgtat atttgagtat
    14761 tttgctacag agtcatactt taaaaaatat gatttttcta ttgatgaaat ttctaatggg
    14821 ttaattggta atacaaatga ttgggggata gatggatttt acatagttat aaataacaaa
    14881 actattcaaa atattgaaga actaaaagat atggaatttt cgcgaaattt cacagtagaa
    14941 ttatatatat tacaatataa aaattcaaat tcatttaaag aaactgttat cgacaaattt
    15001 attgttgttt ctccttatat ttttgatctt gaaaaattta aggaaagaga ttcattcgat
    15061 gttttacctc aaatattaat agaatacatt gaaatttttc aatatatcat taaaacgtat
    15121 gcttcaaaat ttcctaatgt aagtattaac tttgttcatg ctagtaaagg agaaactgat
    15181 agagttaatg gaaataaaaa attaaatcaa aattatatga aaaaagttga agacttaaag
    15241 caacatgtta ataattattc tttaggaaaa aatacatctt gttcttatga acttattggg
    15301 attgaagaat taaatatgct gtctcaacta caaaagtctt attctggaga tttaaaggta
    15361 agtcaaaatc ccatatatgt aagttttggc gatgagaaaa ctaaacaaaa aggatacatt
    15421 gctacagttt cactaaaaaa ctttttcaat tttttagtgg attatgatga aaataataat
    15481 ccaattttaa aagagtatct atttgaatct aatattcgtg attatca
//
