LOCUS       AP027809                5441 bp    DNA     circular BCT 03-NOV-2023
DEFINITION  Escherichia coli 2017.12.01CC plasmid p2017.12.01CC_2 DNA, complete
            sequence.
ACCESSION   AP027809
VERSION     AP027809.1
DBLINK      BioProject: PRJDB14867
            BioSample: SAMD00562598
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 21.0.3
            Genome Coverage       :: UN
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.12.01CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.12.01CC_2"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(1229..1420)
                     /gene="rop"
                     /locus_tag="VEE57_44740"
     CDS             complement(1229..1420)
                     /gene="rop"
                     /locus_tag="VEE57_44740"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BEC72758.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCPRTFGDAGEHL"
                     /name="rop"
     gene            1561..1809
                     /locus_tag="VEE57_44750"
     CDS             1561..1809
                     /locus_tag="VEE57_44750"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC72759.1"
                     /translation="MDAAGLPGEPVARSGRLPTLAPPLLRQLAAIGNNLNQTARKVNSG
                     QWSSGDRVQVVAALMAIGDELRRLRLAVREQGARDDS"
                     /name="hypothetical protein"
     gene            1799..2284
                     /locus_tag="VEE57_44760"
     CDS             1799..2284
                     /locus_tag="VEE57_44760"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC72760.1"
                     /translation="MIVKLHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLPR"
                     /name="hypothetical protein"
     gene            2488..2670
                     /locus_tag="VEE57_44770"
     CDS             2488..2670
                     /locus_tag="VEE57_44770"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC72761.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIRDAIALFTASTE"
                     /name="hypothetical protein"
     gene            2707..3006
                     /locus_tag="VEE57_44780"
     CDS             2707..3006
                     /locus_tag="VEE57_44780"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC72762.1"
                     /translation="MMQHRKDVLKLAGNTGMMLLGMVIFLFTVSGGTLWYLGGMIQANL
                     EEIRKQNETSHKLNAKTWYVEFVQDGNRKFLVLPYGKSAEVIPFQGKEWVHLKE"
                     /name="hypothetical protein"
     gene            3013..3246
                     /locus_tag="VEE57_44790"
     CDS             3013..3246
                     /locus_tag="VEE57_44790"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC72763.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="hypothetical protein"
     gene            3872..4135
                     /locus_tag="VEE57_44800"
     CDS             3872..4135
                     /locus_tag="VEE57_44800"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC72764.1"
                     /translation="MSVLYEFEKNYLQLISEYKEEIKFASSLQEDLRKERAKFFSETLK
                     RVHQTLSESQVDNEVASKWIKELVGSYTKSLDLSEVLLKSIH"
                     /name="hypothetical protein"
     gene            4476..4745
                     /locus_tag="VEE57_44810"
     CDS             4476..4745
                     /locus_tag="VEE57_44810"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC72765.1"
                     /translation="MRRVADIKGKKQGNCKKIMSGLVGFKYSSGDSCADLLFFNGKLEQ
                     IRKIYEVFDRDRDRLEYLNYMMKKERVIFEREFERLLGEKNGDF"
                     /name="hypothetical protein"
     gene            4732..4890
                     /locus_tag="VEE57_44820"
     CDS             4732..4890
                     /locus_tag="VEE57_44820"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC72766.1"
                     /translation="MGIFSQIGSALLNCISKPVDALCEWAKSPLKDREYRREEAQRSLN
                     ISVTWMS"
                     /name="hypothetical protein"
     gene            4928..5131
                     /locus_tag="VEE57_44830"
     CDS             4928..5131
                     /locus_tag="VEE57_44830"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC72767.1"
                     /translation="MKLNAEYEREKKRKLEVDLEVRRVREIEKLVAEIEENGESHRNLR
                     GWSEQRQLCTLQATNEIKHRSD"
                     /name="hypothetical protein"
     Typing          1070..1152
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.81"
                     /coverage="100.0"
                     /evalue="1.76e-34"
     Typing          complement(500..1044)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1455..1582
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.38e-54"
     Typing          2242..3350
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.005"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggagcgttt tcatattgtt gatgcgacat cttctttata tattacaagt gatgagaaag
       61 agcgattgtt gctgaaaaat attaattagt atttttgtga gtaatcgcga tcattatttt
      121 taatggtcgc attgtttgtt cttgcggttg ctaatcatta gttttttgcc ttgtggttga
      181 aaactgagct ctatggctat ggccgaggag tgggagtgaa aagattcaag gaacttcctg
      241 agatcctttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccaacgg
      301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtatct gcgctctgct
      361 gaagccagat accaaatact gtccttctag tgtagccgta gtcaggccat cacttcaaga
      421 actctgtaag catctggaca aatctcgctc tgctaactcg gttaccaatg gctgctgcca
      481 gtggtgcttt tgcatgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      541 agtggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      601 ccgggccgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc tgcggtgctg
      841 gcttcttccg gtgctttgct ttttgctcac atgttctttc cggctttatc ccctgattct
      901 gtggataacc gtattaccgc ctttgagtga gctgacaccg ctcgccgcag tcgaacgacc
      961 gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc ttatgtgaca ttttctcctt
     1021 acgctctgtg cggtatttca cacccggcat ggggcacttt tcatacaatc tgcattgatg
     1081 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca gggcttcgcc
     1141 ccgacacccg ctgacgcgcc ctggcgggct tgtctgctgc cgaaaacgct cgcggacaag
     1201 ccgtgagcgc ctccgtcagc tgtatgtgtc agaggtgttc accggcatcc ccgaacgtgc
     1261 gagggcagct gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacag atgtctgcct
     1321 gttcatccgc gtccagttcg ttgagctttt ccagcagcgt taatgtttgg cttctgataa
     1381 agcgggccat gttaagggcg gttttttcct gtttagtcac tgatgcctcc gtgtaagggg
     1441 gggatttctg ttcatggggg taatgatacc gatgaaacgc gagaggatgc tcacgatacg
     1501 ggttactgat gatgaacatg cccgattact ggaacgctgt gagggtaaac aactggcggt
     1561 atggatgcgg cgggtctgcc tggtgagccg gttgcccgct ccgggaggct gccgacactg
     1621 gcaccgccgt tactgcgtca gctggccgcc atcgggaata acctgaatca gacagcccgt
     1681 aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtagtggc cgcactgatg
     1741 gccatcgggg atgagctgcg ccggctgcgt ctggccgtca gggaacaggg ggcgcgggat
     1801 gatagttaaa ttgcatgcca gggggaaagg cggtggaagt ggcccggttg attacctgct
     1861 gggaagggag cgaaaccggg aaggggcaac ggtgcttcag ggtaatccgg aagaagtccg
     1921 ggaactcatc gatgccacgc catttgcgaa gaaatacacg tcaggtgttc tgtcgttcgc
     1981 ggagaaggag ctgccgccgg gaggacgtga aaaggtgatg gcgagctttg agcgtgtact
     2041 gatgcccggt ctcgaaaaga atcagtacag catcctgtgg gtggagcacc aggacaaggg
     2101 acggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa aacgcctcca
     2161 gccgtactac gaccgcgcag acaggccgag aattgatgcc tggcagacgc tggttaatca
     2221 ccattacggg ctgcatgacc ccaacgcccc ggagaaccgc cggatactga ctctccctag
     2281 ataacctgcc ggaaacgaaa caggcgcttg cagagagcgt cacacgcggg atagacgcac
     2341 tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc actgaggcgg
     2401 ggctggaagt ggtcagggtg acgcgaagca gtatcagcat tgcagatccg aacggcggga
     2461 agaatatcag gctgaaagga gcattttatg agcaatcttt tacagacggg cgcggagttc
     2521 gagaaaaagc tgaaagagag agccgaatct acagagacaa tgctgaacga cgagttcagc
     2581 aagctcgaaa aatctgtaag caaggctgtg acatcaaacg agacgaaaat cagagacgct
     2641 atagccctgt tcacagcctc gacagagtaa tcgctggaaa aacaccggga aggagtgaaa
     2701 gaggcgatga tgcagcacag gaaggacgtg ttaaagctgg cagggaatac gggcatgatg
     2761 ttactgggga tggtcatttt cctgtttacc gtgagtggcg ggacgctctg gtatcttggc
     2821 gggatgatac aggcgaacct ggaagaaatc aggaagcaga acgagacgtc gcataaactg
     2881 aacgcgaaga catggtacgt ggagtttgtg caggacggga acaggaaatt ccttgtcctt
     2941 ccgtacggga aatcagcgga ggtgattccc tttcagggga aagagtgggt acatctgaag
     3001 gagtgactca gagtgacaga gctggaaaca catttgctga acgccttaga gcagctgcaa
     3061 caggactata tgcagcggct gagcgaatgg gagagcgcct tcgtggaatt gcagaagatg
     3121 ttttcgctta cgcaacggga caacgcgatg ctgaacgagc gggtcatgca gttgagtcag
     3181 caggtgcagc acttgagcga gcagacagaa cgcttgagcc agttatacag cgagaactgg
     3241 agataagaga ggcacggctg atacaggagc gcgaacatgc gttatccctg gaacgggagc
     3301 gtcagccgga aatacaggaa cgcacgctgg atggtccttc gctgggatgg tgaaaccatg
     3361 aaaaatagca gcttcagtga gttaagaggg ttaatgtggc ctgtaccatc tggctgcata
     3421 gctatgcatg tggttaaaat ttatcgggtg ccatcgcggc actttttccg gaggtgtgtt
     3481 gccatttttg cccgttcatg ttgccgtgat cgcgctgaac gcgttttaga agcggtgcgt
     3541 gtaatgcgac gttatggtaa atggatgagt taggcctaag tcatgacaga gataaaacga
     3601 ccagtattaa aaatggcttt agaaaacata atcagatttc tagtgactct atagagagat
     3661 ggggagactc tatgtcagca taggaagaga gttggatgtt gagaccaaag gaagcgtact
     3721 tattctatga ataacgatat gagtttatat aatgtaatat cacgggctgt tgaagcgtct
     3781 ggtgccgatc attcaattaa tgaaaaattg acaaaatgtc ttgaaaagag agttagttga
     3841 ttatgtcagc attgcgcatc taaagaccaa attgtctgta ttgtatgagt ttgaaaagaa
     3901 ttatttgcag cttatctcag aatataagga agaaataaag tttgcttcct ctttgcaaga
     3961 ggatttacgt aaagaacgtg ctaaattctt ttctgagaca ttaaaaagag ttcatcaaac
     4021 tctaagtgag tctcaagttg ataatgaagt ggcatcaaaa tggattaaag aacttgttgg
     4081 tagttatacc aaaagcttgg atctaagcga ggtcttgttg aagagcatac attagataca
     4141 attgcttgta ttcgcgcgga ggctaaatta aataaaccat ctattgagcc gggcaataat
     4201 taattatggc cggggataga catataaata gtttgcttta tgttcttgtg actatattag
     4261 aacgtaaagt gaatgatact ttaaattgtc ttcgtcggaa cttcctgaga tattggctgc
     4321 tttacgagag gcaaggagtg ccttgtatcg cgcagcatca atgaagagcc tcagttatat
     4381 tcggcattgt ttttatatga aagaaatgaa gggctgttaa gtctttcatt tgagagaagt
     4441 actctatttt tattaactct aatttggagt gtatattgag gagagttgcg gatataaaag
     4501 gtaaaaagca gggtaattgc aaaaaaataa tgagtgggct tgtggggttt aaatattcaa
     4561 gtggcgattc ttgtgcggat cttttgtttt ttaatggtaa gcttgaacag atacgtaaaa
     4621 tatatgaagt ctttgatagg gatagagata gacttgaata tcttaactat atgatgaaga
     4681 aagaacgtgt tatttttgaa agagaatttg aacggctttt aggtgaaaaa aatggggatt
     4741 tttagtcaga ttggtagcgc tttgcttaat tgtattagta agcctgttga tgcactctgt
     4801 gaatgggcta agagcccatt gaaagatagg gaatatagaa gggaggaggc tcaaaggagt
     4861 ctgaacataa gcgtaacatg gatgtcttga cggccaagaa gcgtgctgag catgaactca
     4921 gaatgcagtg aaattgaatg ctgaatatga gcgagagaaa aagagaaaac ttgaagttga
     4981 tttagaggtt cgtcgcgttc gcgaaataga aaagcttgta gctgagatcg aggagaatgg
     5041 agaaagtcac aggaacttga gaggatggag cgaacaacgg cagctgtgca cgttgcaggc
     5101 aactaacgaa attaaacata gaagtgatta attctattgg gaacatgcag cttgatttaa
     5161 aggagcgcgc acagaaattt gtctatgaga aagctgtaca atataagcgc ctgcaagatg
     5221 aagcaacgga aagagctatg aatgatctta taagaattga ggaaaagttt ggtgataatg
     5281 agagagctaa agatatttta attcgtgcag ttgatactaa gatggggaat gttattgata
     5341 cgtcaactcg gtttttggaa gaattaaata gagatattgt tagtttaaac caaagtattg
     5401 atagactgac taatcaggac aaaaattcat tgagagtcat t
//
