LOCUS       AP027720                2101 bp    DNA     circular BCT 03-NOV-2023
DEFINITION  Escherichia coli TBU0218_10-1CC plasmid pTBU0218_10-1CC_4 DNA,
            complete sequence.
ACCESSION   AP027720
VERSION     AP027720.1
DBLINK      BioProject: PRJDB14867
            BioSample: SAMD00562614
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8
            Genome Coverage       :: 347x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TBU0218_10-1CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pTBU0218_10-1CC_4"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2018"
     gene            complement(487..753)
                     /locus_tag="VEE66_45770"
     CDS             complement(487..753)
                     /locus_tag="VEE66_45770"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12407.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(757..1722)
                     /locus_tag="VEE66_45780"
     CDS             complement(757..1722)
                     /locus_tag="VEE66_45780"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12408.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="hypothetical protein"
     Typing          complement(1098..1329)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 agggaataaa aacaaaaccc tgtcttacag gtggatggcc tcacttcgtg aaaccatcca
       61 ccccgcataa gttttttaat cggtggtgtc gtcaatcact aaatcggaac cccaaagggg
      121 acccccacga tttagcattt gacgagatta actctcggag atggcatcat gcttttaacg
      181 gactttaaaa aacggtgaac ggatgcggat tttaatccgt gtctgtgagg cgttgagcaa
      241 cgagcaaagc gaggcggtag aaagagagcc agcggcaggc tcaggacgat aatccgggga
      301 aaatgtacca ctcgcagagt gttaacattt gacatcggat tgtctatcct gcattggtgt
      361 cgagttggca aggctgagga taatgctgag gataatgctg aggataatgc aggatagaca
      421 gagcactaga gattgcatgc aatctcgtac ctatcacctc agcctgccgc tggctctttt
      481 ccagaactaa gaatgcgttc ttaaaaatcc aaacttgata ccgtttaaat ctattttcac
      541 aaaaagagtt tttaccctct cgtttgtttt ctgaatatat aaaattaatt catcctcaaa
      601 accttcgtgc ggatgaaaac caaatgtgcc aaaacttaat ccatcaataa gcaaaataag
      661 gtttttaaac ctgtcaggaa tcaccgcttc acttcttaaa aaaaataaac caatagtctt
      721 atttccattc aggtattcaa tattaatatt catacgttaa tccgatttat ccttctcagg
      781 ggcgcgtttg taacgccgtt tacctgaatc ccagacaaac gccgtccgct tcccgtcatc
      841 agtgccatcc cctacatcgt cggcaatgac aagatcctca ttggtttctc gatccaactg
      901 gaggacgttt tttagcgccc caccggtcga gataaaacgg cgcttgtgaa gctgccgcgt
      961 cagctcaaga aaccactcag gatcgtttgc catatcttcc ggtttaacgg agtatttcag
     1021 cgtttcagca accgcgcttt gcagttgctc ggcaacgttg gccacaacct cacctgtctt
     1081 agtttttact gcccgaatat cgatattcgg ctcatagttc acccgcaagc aatcgcgcca
     1141 gagttctacc caacgttcgt gcttaacgta gttcttccct ttaaaccaag aaggttgcac
     1201 catcagcaga cagtgaaaat gcggatgtgc gctgccatcc ttacctcgcg tcacctccgt
     1261 agccctgatc cacccctgaa caggtgatag ctcctttcgc ttttccattc gcttaaacgc
     1321 cgcattcatt gctgtaagga ctgttccaag ttcacctatc tcgcagttcc tgacagttaa
     1381 cgtcagaaac aaccatcggg aagacgggta atccacaacg attttcggta gagcctgata
     1441 aaaacgggct tgccacatga gggttcttct ccactggcag acagggcaat gacggacacg
     1501 gcaaaattgc gcactacgaa gccgtaggcg cgtttctccg gtttccatga tggtagacca
     1561 accaaaacgt aacaaaccgc tacaggaggc catacggagt gcatagcgct cgaattcagc
     1621 agcagtctgg tagatacccc cgacagaatc ggtaagcgtg cgctgggtat cccaaactgc
     1681 atcacggggg ctgtagtccg aaaggaattt atcttctgac attaagcctg ctccgatctg
     1741 ggcaggctgt gatttgatgt ggtatattca tcacgtacct gtgcattgcg agccagtaca
     1801 tgtcaccaga gtttgccgct gaggtgacac attcgaaatg ttggggagtg ttgacgcact
     1861 ccccaacgcc gattaaaaac gcctcaattc tgctccattt tctttgacct gtcaaaaccc
     1921 actcaaagcc tcgtcattac tgggctaaac cactatttca tatccgccat ttatccctta
     1981 agtataaata caaggatcac gcgcgagaat tgcgcaaaaa ataaccacaa ttttaacccc
     2041 ccaaaacaag cacccccgaa cggcttaccc caaccttttc cacacacccg atcaccaaaa
     2101 c
//
