LOCUS       AP027719                2442 bp    DNA     circular BCT 03-NOV-2023
DEFINITION  Escherichia coli TBU0218_10-1CC plasmid pTBU0218_10-1CC_3 DNA,
            complete sequence.
ACCESSION   AP027719
VERSION     AP027719.1
DBLINK      BioProject: PRJDB14867
            BioSample: SAMD00562614
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8
            Genome Coverage       :: 347x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TBU0218_10-1CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pTBU0218_10-1CC_3"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2018"
     gene            21..830
                     /locus_tag="VEE66_45740"
     CDS             21..830
                     /locus_tag="VEE66_45740"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12404.1"
                     /translation="MGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFAR
                     SEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQST
                     YAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKD
                     VEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKG
                     LSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="hypothetical protein"
     gene            complement(900..1130)
                     /locus_tag="VEE66_45750"
     CDS             complement(900..1130)
                     /locus_tag="VEE66_45750"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12405.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKYNYHLND"
                     /name="hypothetical protein"
     gene            complement(1117..1620)
                     /locus_tag="VEE66_45760"
     CDS             complement(1117..1620)
                     /locus_tag="VEE66_45760"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12406.1"
                     /translation="MAKRGAGVNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPY
                     VKLYIADIAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKN
                     IIAALVDKQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVM
                     DDEC"
                     /name="hypothetical protein"
     Typing          4..827
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.273"
                     /coverage="96.0"
                     /evalue="3.95e-166"
ORIGIN
        1 ccaaatcaac ggatcaggca atggggcgtg tgcgggagct gctgccggaa aaacgccgta
       61 aagatgcggt actggccgtt gaatacgtta tgacagcaag ccctgagtgg tggaaagagg
      121 ccacaccacg gcagcaggcg gagtttttcg cccgctctga gcaatggctg gagaagaaat
      181 acgggaagga tcgtgttgtg gcggcggtgg ttcaccggga cgaggccacc ccgcacctgt
      241 ctgcgttcgt ggtgccattg acgcaggatg gacggctgtc cgcgaaagag tttattggcg
      301 ggcggtcaaa gatgcgtgaa gatcagagca cctacgctga atcagtaaaa aaactgggtc
      361 ttgagcgcgg catcgaggga agccgcgcca cgcatcagac agttcagcac tactacgaat
      421 cgataaaccg tggcacccgt agccaggtat cgatctcgcc ggaggcgctg gagccgcgtg
      481 tattgcggaa gggcattttc accaaagacg tggaagatca ggcagccata gccaaacgtc
      541 taagccacgc agtgaacgat ggctttgcag gaaccatcgc tatggcctct cagagcgccc
      601 agaacgcaaa aagagcgcgt gagctacaga agactatgga tgcgcagcag aaacgcctac
      661 agagcgtcac agagcctttt aaggggcttt ccagagagca gatgaccgaa atcctgatga
      721 tggctcagcg gtttaagcag caaaaccagg aaaaggaaaa acagcagcgt attgaacgtg
      781 aaaaacagcg acagacgcga tcgcgtggca tgggtggtat ggagcgttag ttttgatgcg
      841 atgcaggata gggctaacgc cgcaaagtga cgtcatttgc ggcattcacc acgaaaaaat
      901 taatcattca gatggtaatt atattttttt aaaatagaaa aagctgcgtt tttctgcttt
      961 ggtgacaaac ccttagttga gtatttatta ccgctagttc tacgatatat gctgtaaaca
     1021 ctgcttaaaa aagtacgctc ccaaacagtc agaacctcat tcttttttag tgaatcatgc
     1081 agcattagcc tgtctacatt tttaaaaagt ttatcttcag cattcatcat ccattacctt
     1141 cgtctgcacc gttcttccat tggtgtcata actgatcttt aactcaattt tctttcttct
     1201 ttcgtaaatt gatggccagt ctccccgtgc aaaaagaaag gggttagctt caagttcccc
     1261 acgccctgcc cggaagaata tttgtttatc tacaagagcc gcaattatgt ttttaaaggt
     1321 ctgatattca atatgaaccc tgggctgtat ggttttttca aatctagttt ttgatacaga
     1381 cacaaaccca tcccatccaa tcagcattgc caattgtaaa agcacatcat tttgagattt
     1441 tgataaacca tgaatcatcg caatgtctgc aatgtatagc ttgacgtagg gaggttcggc
     1501 tgggatggaa aaggtctcga tcttagagtc ttgttttacc tcaccgtttt catccatgta
     1561 tgtatgccgt tcaagcctag aaatcttttt cgtattcact ccagccccac gtttcgccat
     1621 gatttagtac tcgcatttaa gtactaaaga gacatacagt caagtccttt gtatcaagta
     1681 cgattagtac ttgaggtgag tacgattagt ccttgaggtg agtacgatta gtacttgagg
     1741 tgagtacgat tggtacttga ggtgagtacg attcttcgct ctagcccttg gtacaagcgg
     1801 gttcccatat ctatatctat atattaaggg ggaaaataag ggctgaaaaa cggcattttt
     1861 agacactgaa aaactgcctt tttttagcaa ggagtgtacg catttctatg tacggggagt
     1921 ggaaattccg cttcctcgct ttggctgttc gttcctcgtc gcgttgctcc tgcggtacgc
     1981 ctcagccaaa gctgcggcga gcggttcctg ctctttcaaa gacgatttca ttgcctgatt
     2041 tcgtcaaggg cgagcgtagc gagtgccatt tacccttgac gacactccgg ataaaacagg
     2101 cttggcagtg gtggggggtg gcgcgaagcg acgccttcca cctgccagac cgccgttgat
     2161 tttgaccttc tggcagcttg ctgcccgaac gattttgcgt agcaaagtct agtgagtaca
     2221 ctatcatctt gatagtggcc cgccggaggt atcgcaatgc gctcccccgc ctggctgaat
     2281 gaatgccgga atcagggggg gacatggcga actacgcgat aatgcgctgc aaaaagctga
     2341 cgggaatggg aagcgtggcc agcgcgttgc agcactgcta ccgagagcgt gagacgccga
     2401 acgctaacgc tgaacggaca ccagaaaact actgctctgt gt
//
