LOCUS       AP027718                6413 bp    DNA     circular BCT 03-NOV-2023
DEFINITION  Escherichia coli TBU0218_10-1CC plasmid pTBU0218_10-1CC_2 DNA,
            complete sequence.
ACCESSION   AP027718
VERSION     AP027718.1
DBLINK      BioProject: PRJDB14867
            BioSample: SAMD00562614
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8
            Genome Coverage       :: 347x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TBU0218_10-1CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pTBU0218_10-1CC_2"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2018"
     gene            complement(668..877)
                     /locus_tag="VEE66_45670"
     CDS             complement(668..877)
                     /locus_tag="VEE66_45670"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12397.1"
                     /translation="MVDCTQPPPAEVSTMSKHHRKHTKQAYKAVKDVPVIIKRSLAELR
                     KIAEEEEQARWEELQLAHQLSKWL"
                     /name="hypothetical protein"
     gene            1545..1895
                     /locus_tag="VEE66_45680"
     CDS             1545..1895
                     /locus_tag="VEE66_45680"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12398.1"
                     /translation="MTQRKQSNPKTLDERIEQARKRFREQCESPNEETMRRLLVIMPES
                     LNSVVESQVGALAEDCSDNTAIGLGVQLFVRISRQFPALPAYVVVDVVMRLGNLHKMRD
                     QFVEALEHLEMI"
                     /name="hypothetical protein"
     gene            1909..2427
                     /locus_tag="VEE66_45690"
     CDS             1909..2427
                     /locus_tag="VEE66_45690"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12399.1"
                     /translation="MNIELESVNADFLDTRTIEEQVQDFIKAEMQQLEQQQLRTDSLNK
                     ARAVAKRDYLAGCKRHLCGHAVNVMATAEQLIREGWKVDLQTFQALNGYIDMYLIPTDA
                     MLKAGIRKAQREAVASLKLRHETAMKVALEQIKERAAALYDKLTVEQAIAEQHRRRDEI
                     ISKLVLGGR"
                     /name="hypothetical protein"
     gene            2823..3122
                     /locus_tag="VEE66_45700"
     CDS             2823..3122
                     /locus_tag="VEE66_45700"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12400.1"
                     /translation="MDSNSRTTNIIVDGVMVHLSPTAYKLLMHMINNPAGQYFTGELRS
                     QFGKCATRNLRPYFEELQSYKLIEPVFVYGRVIISRIAEQVDTAFGMPVFERIE"
                     /name="hypothetical protein"
     gene            3119..4081
                     /locus_tag="VEE66_45710"
     CDS             3119..4081
                     /locus_tag="VEE66_45710"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12401.1"
                     /translation="MSGKRQVVESIANMLKEVEERLLDQSEVSRMAMPDFDAYDDALLT
                     AIRLHPEAAIECGLLCPVVKLLSYAPRDGKPKPDTLLALITNLYSSRYGHGFRKLHNGY
                     QLLQPVPIVAANKLDWTYTGYPIDGYGDWFATDFERSKWSLLIHTLIGYFPGLELPRAW
                     SKMFDAERNMRRQGYKDFVHPLKEHKGMSEVSYVDDQGYVSCGVHNPRGCGLINVEPVN
                     HVVDHMYGNTATLQPGIRAALQLRPVLPDVNKVNRHANHAIGDFLCWGDFTGYQPEYFN
                     GDWDDLIWKGWQHMMNQVNQPVGGFRFEDPALWCLALSK"
                     /name="hypothetical protein"
     gene            4092..5576
                     /locus_tag="VEE66_45720"
     CDS             4092..5576
                     /locus_tag="VEE66_45720"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12402.1"
                     /translation="MNMVDKDTTTTTTTIQQQPQQQQQAAVKPTFWQALRQYIRHLNDT
                     GCYCEPSSSKGYVVYYADNTVLAGGFDQTWVAVVRWMKAHHIYDIPDSRKQKENVVHDV
                     WAGLPLVSGVVLRPDVAEPMVPGKHGDWQRNLWKPYSYSTAPQAHQRVDLVMPYLTLVE
                     FLVPNPAERKIVLQFYAHALRPDTAADKPSFGLLFTGPEGDGKSFTFDTVPTLVLGEGR
                     VHHAQQGVNALATALETACYYDKALCICDDFDDANTATVERLKCVITRIMAQIKRLYQD
                     IQQVPIYTRHIFIGNSHNPIYFGSGTDRRYFAPAYCPEKDPAMIQLIDDYHKLLKADPV
                     YRDAWYWYMTTQVDISDFNPHVLVETDNHRRMVSGCITDVQSQFKAMLDELKPDLVTKY
                     WYQHHLIKMFGDDLTAATVDKQWKAISGYLRDGAGWQHVQFRLPGAGGARGSRIWGFVR
                     PGITQAQIKAMLAAMDPADLEVYTLSSLGDKGWYAS"
                     /name="hypothetical protein"
     gene            5821..6015
                     /locus_tag="VEE66_45730"
     CDS             5821..6015
                     /locus_tag="VEE66_45730"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEC12403.1"
                     /translation="MHWSGVAVVNAPLTALTLARYAIVLPATVYIAVALFKGDPVDVRW
                     LWKASERLTQIVPRFNTCK"
                     /name="hypothetical protein"
ORIGIN
        1 gtactaaagc acctcaggaa cgtgagggat acgctacttt agccaccagc ctgagcaaag
       61 gcgacatgtt attgttggac tctctcgacc gtatcagccg tgacccgtgg accttacagc
      121 gtactgttgg tgacttgctg aacatgggta ttgagttgca ttgtgtgaag ggtgaagacg
      181 aggcggtgtt aaagactgcc aacgataacc ctgtactcgc tccgactctg gtcggtgtcc
      241 tttgtactgt tgcaggtatg aagatcgagg aggtcaggac aaagactacc aacgcgctca
      301 accgtgtacg caaagaggca gccgaaggta agagcgataa acgtccgggc cgcaaagcca
      361 actatgaccc caaagaggtg tgcgagatgt tggggcaagg tattccggtg aaagttatca
      421 gcgaaagatt gggtatcagc cgccgcactg tgtactactg gcaagctgag ctagagggcc
      481 atagccgcaa caaaggcata gctcgtaaag agttgatgat gtgaggccta aatagcttcg
      541 tataatgatc gctatacgaa gtaaagagcc gttatagttc aacattgcaa ctattagctg
      601 actaactgct tgtacatctt cacgacttcc tgcacgctct ggcagccacg cgtctagcgc
      661 atctccgcta tagccatttg ctcaactggt gggcaagctg tagctcctcc caacgggctt
      721 gctcttcctc ctccgcgatc ttacggagtt cagccagtga ccgcttaatg ataacaggca
      781 cgtctttcac ggctttgtat gcttgtttgg tgtgtttacg atgatgtttg ctcatggtgc
      841 tgacctctgc gggagggggt tgggtacagt ctaccataaa tctcttataa agttacttgc
      901 taatcttaaa gattgggtgt attatacaaa tcatcgagag ttagagcggt tcaggaggtt
      961 atctctcagg tctgtttcat tcatgtttgt tcatgttcaa tctctttcat taatgagttc
     1021 ctctatttgt tcaggccttt actttgggtt atcccgccag tgatggcggg tatctttcct
     1081 ttcgaaagtc aaatcacgga tagcatctct caggcacaag attccacctg taaacggtag
     1141 tcggttgggc aaagttagcc tctcaggttg cactaacttc aatcttgttg tgggaacacg
     1201 cgcccggcaa cggcaagtcg cgcggcggtg ctggtttaac ttgttaatct cgtaacgcta
     1261 actacttagt cgccagcatc ttccgcaagc caccagctaa gccttaatgt tggtggggta
     1321 agtacccaac aagcgctgac tccgtttaac aagtggtatc ttcctagagg atacccagcg
     1381 gataaactcg tttcagagta gtaaatccac catgttcaca ttcctctttg gcctccttcc
     1441 ggcgctccta gtcgctggtt gatcgaggcc atctttgtat cttcacgact gttcaggagt
     1501 gtatgtggtg ggcaacaaac aaacatgcat aaaggtatac aacaatgaca caacgtaaac
     1561 aaagcaaccc gaagacactt gatgaacgta tcgaacaagc ccgtaagcgc ttccgtgagc
     1621 agtgtgaatc tcccaacgag gagacaatgc ggcggttact ggtgatcatg ccggagtcac
     1681 tcaatagtgt ggtggaaagt caggttggcg cactggcaga agattgcagc gataataccg
     1741 ccatcggttt gggcgtgcag ttgttcgttc gcatcagtag gcagtttcca gcacttccgg
     1801 cctatgtagt cgtagatgtg gtaatgcggc tggggaatct tcacaagatg cgcgaccagt
     1861 tcgtcgaagc acttgagcat cttgaaatga tctaaccgga ggtgacacat gaacattgag
     1921 cttgaatctg tgaacgctga tttccttgat acccgcacca tcgaggaaca ggtacaagat
     1981 ttcatcaagg cagagatgca acaactggag cagcaacagc tacgcactga cagcctgaat
     2041 aaagcccgtg cagtcgctaa acgtgattat ctggcaggtt gcaaacgtca cctgtgtggt
     2101 cacgccgtca acgttatggc aacggctgag cagttgattc gtgaaggctg gaaagtggat
     2161 ttgcaaacgt tccaggcttt gaacggttac attgacatgt acttgattcc gaccgatgca
     2221 atgctcaagg ctggcattcg taaggcccag cgtgaagcag ttgcgagcct taagctacgt
     2281 catgaaacgg ctatgaaggt ggcacttgaa cagatcaagg aaagagcggc agcgctttac
     2341 gacaagctga cagttgaaca ggctattgct gagcaacacc gccgccgtga tgagattatt
     2401 tcaaagctgg tgctgggtgg gcgctaactt tggacaatag gagccaataa gccccgaggg
     2461 tgacgggcta cggtaagagc ctccgaccgg aggccatcat cagtacagtc ttgttagctc
     2521 atttgtcagg tatacgtttc ctgttccgac tctcagggtg atcctgtgag ctgtgtaggc
     2581 ggtgtatgac tccgcacggg atagctgtta tcgcacaact atcaaacgtc accaatggcc
     2641 tgtgttgacg ctggttaggc gtagcgccgg gcttatgcgc tgagctacaa gactagttgc
     2701 aatactattc gaccaatctt gctgagaggt cgcaccggaa gcaacatctc caaagtgcag
     2761 gttaaaggct aagccgatta acctgtgaac agtataaacc gattaactca tagaggtaaa
     2821 caatggacag taacagcagg actaccaaca tcattgttga tggtgtgatg gtccatctat
     2881 ctccgacagc ttacaaactg ttgatgcaca tgattaacaa cccagcaggt cagtatttca
     2941 cgggtgagct acgcagccag ttcgggaagt gtgcaactcg caacttacgg ccttactttg
     3001 aggagctaca gagctacaag ttgatcgagc ctgtattcgt ctatgggcgg gtgattatca
     3061 gtcgcatcgc tgagcaagta gacaccgctt tcgggatgcc tgtgtttgag aggatcgaat
     3121 gagtggaaag agacaagtag ttgagtctat agcaaacatg ttgaaagagg ttgaggagcg
     3181 tctactggac cagtcagagg tgagccggat ggctatgcct gactttgatg cctatgatga
     3241 tgccttgtta acggctatca ggttgcaccc tgaggcagct atagagtgtg gcttgctgtg
     3301 tccggttgtc aagctgttgt cgtatgcacc tcgcgacggt aagcctaaac cggatacttt
     3361 gcttgcgttg ataaccaacc tgtatagcag tcgctacggg cacgggttca ggaagttaca
     3421 taatggctac caactgctcc agcctgttcc gattgtggca gctaacaaac tggattggac
     3481 ttatacaggc tatccgatag atggttacgg tgattggttt gcgaccgact ttgaacggtc
     3541 aaagtggagt ctgttgattc atacgctgat tggttacttc cccggccttg agcttccacg
     3601 cgcttggtca aagatgttcg acgctgagag gaacatgcgg aggcagggat acaaagattt
     3661 cgtccacccc ttgaaagagc ataaaggtat gtcagaggtg tcttacgttg atgatcaagg
     3721 ttacgtcagt tgcggtgttc ataacccgcg cggctgcggc cttatcaatg ttgagcctgt
     3781 caaccatgtt gtagaccaca tgtacggcaa tactgccacc ttacagccgg gaatacgtgc
     3841 cgctttgcag ctacgccccg tcttaccaga tgttaacaag gtcaatagac acgctaacca
     3901 tgctattggt gacttccttt gctggggtga cttcacaggc tatcagccag agtatttcaa
     3961 cggtgattgg gatgatctta tttggaaagg ttggcagcac atgatgaatc aagtcaacca
     4021 acctgttggc ggtttccgct ttgaagatcc tgctttgtgg tgtctggctt tgagtaaata
     4081 ggtgaacaag tatgaacatg gtagataaag acacaactac aacaactaca acgatccaac
     4141 aacaaccgca acaacagcaa caggcggcgg ttaaaccgac gttctggcaa gcgctgaggc
     4201 aatacatccg acacctgaac gatacagggt gttactgtga gccgtcttcg tcgaaaggct
     4261 atgtagtcta ctacgccgat aatacggtgc tggctggcgg ctttgaccaa acatgggtgg
     4321 ctgtagttcg ctggatgaaa gcacaccaca tctacgacat accggacagc aggaagcaga
     4381 aagagaacgt ggtgcatgat gtttgggcag gtttgccgct ggtatctggt gttgtattac
     4441 gtcccgatgt agctgagccg atggttcccg gcaagcatgg cgattggcag cgtaacctat
     4501 ggaagcccta cagctacagc accgcaccac aggcccacca gcgggtagat ttggttatgc
     4561 cttacctgac gctggtcgag ttccttgtgc ccaatcccgc tgagcggaag atagtgctac
     4621 agttctacgc tcatgcactg cgaccggata ccgcagccga taaaccatct ttcggcttgc
     4681 tctttactgg gccggaaggt gacggcaagt cattcacctt tgacacagtg ccgacgctgg
     4741 tattgggcga gggaagagtg caccatgccc aacagggtgt taatgcgctc gccacggcgc
     4801 tggaaacagc gtgttactac gacaaagcat tgtgcatctg cgatgatttc gatgatgcca
     4861 atacagcaac tgtagagcgg ttaaagtgtg tcattactcg catcatggcc caaatcaagc
     4921 gactgtatca ggacattcag caagtgccta tctacacacg gcacatcttc attggcaaca
     4981 gtcataaccc catctacttc ggaagcggca ctgacaggcg ctactttgct ccggcgtatt
     5041 gtcctgagaa agacccagca atgatccagt tgatagatga ctatcacaag ctgttaaagg
     5101 ctgaccctgt ataccgagac gcttggtatt ggtatatgac aacgcaggta gacataagcg
     5161 actttaaccc gcatgtgttg gttgaaacag acaatcatcg ccggatggtg tccggttgta
     5221 ttaccgacgt gcagtcacag tttaaggcta tgctggacga gttgaagccg gaccttgtta
     5281 caaagtattg gtatcaacac cacctgataa agatgtttgg tgacgatctt acagccgcca
     5341 cagttgataa gcagtggaag gctataagcg ggtatttgcg cgacggtgca ggttggcagc
     5401 atgtgcagtt taggcttccg ggcgctggtg gggcacgggg tagccgcatc tgggggtttg
     5461 ttcgccccgg aataactcag gcacaaataa aggctatgct ggcagctatg gaccccgcag
     5521 accttgaggt gtatacgcta tccagtcttg gggataaagg ttggtatgca tcttgatcaa
     5581 atggttagca gactaagcat tgtcacagtg tcacagctat gtcatagccg gagcggttat
     5641 ctgtgacagc ctctagccca gagatgacgc gggttacaga cgatttgtca catgtcacag
     5701 cacgaccccc gaccctttat atatattttc ctgcgagtta tctcgccagc cgtaggctga
     5761 ggaaagtaaa caccggagcg caaagggagc gaccatcggg agtcccgtag cggagctacc
     5821 ttgcactggt caggtgttgc tgttgtgaat gcgcctctga cagcattaac tttggcaagg
     5881 tatgcaatcg tattacctgc taccgtttac atcgctgtag ccttgtttaa gggcgatcct
     5941 gttgatgtgc gatggttgtg gaaagctagc gaacgcctga cgcaaatcgt tccccgcttc
     6001 aatacctgca aataaataaa taaatacttt acgcaactaa caaatgttta caaatggttg
     6061 acatttcatc aaatcttgcc gccatcaagc atacatcgtg ccaacattac ttgttaaaca
     6121 aatgcccgat aatcgaggtt attcgataga taaagcctat taaatgattg tcaaactata
     6181 aatcacggca agcaactgtg aacaaatggt cagttgtgca caaatccgat ttgttgtatg
     6241 atgttcccaa cagataagga ggtgcatcat ggcggtttat gcttactatc gcgtcagttc
     6301 cgagaaacaa cgcaaagacc ggagtattga gagtcagaag tatgccgtag accaactggc
     6361 tgagcgcttg catgtggtta tcgctgactc tttcgaggat gatggtgtgt ctg
//
