LOCUS       AP027531                4586 bp    DNA     circular BCT 03-NOV-2023
DEFINITION  Escherichia coli LR-41 plasmid pLR-41_3 DNA, complete sequence.
ACCESSION   AP027531
VERSION     AP027531.1
DBLINK      BioProject: PRJDB14867
            BioSample: SAMD00562551
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 21.0.3
            Genome Coverage       :: Unknown
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-41"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-41_3"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            complement(477..596)
                     /locus_tag="VEE32_45800"
     CDS             complement(477..596)
                     /locus_tag="VEE32_45800"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 404,494"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEA56736.1"
                     /translation="MLRKMMAVEVNFEQGAIGPMHNHPHEQLTYVLSGSSSLR"
                     /name="hypothetical protein"
     gene            complement(3133..3495)
                     /locus_tag="VEE32_45810"
     CDS             complement(3133..3495)
                     /locus_tag="VEE32_45810"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 3509"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEA56737.1"
                     /translation="MSRMEQILPWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHW
                     YNLSDGAIEDALYEIASMRLFARLHESQHPGQGGAPISHHQATVRLRESQIQGLLKNDN
                     QLAMLFTLANLFRADR"
                     /name="hypothetical protein"
     gene            3761..3967
                     /locus_tag="VEE32_45820"
     CDS             3761..3967
                     /locus_tag="VEE32_45820"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     4129,3879,3784,3948,3857,4086,3805"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEA56738.1"
                     /translation="MSAMDFKKTRTSIFRYATPAVSTKEIDLLGLLGRTSGSQKRIVTI
                     VLAFAVVGLAIAFLIPQNGPVKP"
                     /name="hypothetical protein"
     Typing          complement(1847..2061)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.33"
                     /coverage="100.0"
                     /evalue="3.27e-96"
     Typing          2041..2529
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.669"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 caaaagctgc agattgaaaa gaatctgaag gacgtggccg agctgaatgc ggatttccag
       61 aaccgtgaat actatctgac ccagttgcaa aaactgtccg tggaagacgt ggaagcttga
      121 acccttcagg tatcagcttt caccgtcact gccggtgaaa aaagacggtc cgggcaaagc
      181 cctgattgtg ctgctggcag gattctgggc ggcctgtttg cctgcggcgg cgtgctgctg
      241 cgtgaggcaa tgctcgcgta atccgttgcc agagccgata acggccgtaa cgccagcaac
      301 agagtaacga cgatattaaa aaggcgctga ttcagcgcct tttttatttc aggaaatcgt
      361 cgcgcaccgg cgtaaaggta tccagaaggg tgccgggctt caggcaatgc agccgtgcat
      421 cacgtgcggc gctttataga gcgtatcgcc cgccgtcacg atctgctttt cttcacctat
      481 cgtaaactcg aactcccgga gagcacatag gtaagctgtt catgcgggtg attgtgcatc
      541 gggccaatcg caccctgctc aaaattgacc tccaccgcca tcatctttct tagcattctt
      601 ccttggtgga tttggcgtac ataaatttta cttaggtaaa ataggacagg gattcctgca
      661 tctaattttt tgctggacat tcatacccgc gatcatcgcg tttattgagt ttattattta
      721 tctatgcgat tccgatgaaa attcgcaaga aaatatggct aactcatagc aaaagacctg
      781 cggggctttt ttgtaccagt ctgtgaggca gatttaccat aacatccctt gcccgcaccc
      841 cctgagaggc cctcagcgcg tttctgccgc gtcctctgcg tttccggcac ctttccctgt
      901 ccatatcgtg aaaaaacgcg cctgatgatt tctgcgcatc tgcatggcta tgcacgaaca
      961 tgcctggccg gaattttagc ggctaaaatc ctgcgaactg ctcaaaaggc tcgctgaacc
     1021 ccatcgataa cggtgaacgg gtgcacagtt tactgtgcgg tcgtgaggcg ttgagcaacg
     1081 agctttgcga ggcggtaggg gttctgagag ccagctgcag gctcaggaga aaacattctg
     1141 ttttcggggc agataccgca aatgacgtca ttttgcggcg tctgccctat cctgcatcgc
     1201 aacgtctaaa ggatgtgagt acaaacgcca gcactttgcg aatcctgccc tcgatctgaa
     1261 caaaagcaaa atttcgtacg tacgaaattc agggtatcag gctgcgcctg atgaaatgtt
     1321 tttggtgccg gcggtactcc aaaaacacat cttgtccgac agttttgtgg tgccggcgaa
     1381 gcggcggtgg ccacaaaaca tttcctgtcc ccatcgattc attcgagaaa cataagaggc
     1441 gaagaagggg tcagtttgga gatcggaaat ttttgtacgt taagcgcatt atttaaccaa
     1501 tcttggttcg taaggggttg attttgcgtc agtggctatc gcttttaagt tgtcgctctt
     1561 gcagtatcgc atttaggtta tccgattaag gtcaaacctc tgaaaatgcc gtatagcgcg
     1621 ggagtacacc ctattgctat aggtaagtct gttcaaaaaa caggcttacc gtacaataat
     1681 tctctatatc caaactgacc cctataactc gcttcacggc cgacaggtgg cccaaaatcc
     1741 accggggcgt gcaggttctc ttcagtaacc ttcgccatta aatcaaaaag gtcatattcg
     1801 ccgggaagct gcgtatcagt cgaatcccgt tgttcatcat attcagatgg catggggtga
     1861 tatccagtag cggagaagtt gttagcgggt ttcggggcgc agccctgaac cagtcacata
     1921 gcactagcgg agtgtatact ggctaaatat gttacggcgg agtggtaatt tatgaaagtg
     1981 cttaatgtga cagggaaaaa tgcggctgcc gggcgtaagt gcaacatgca ggtaaggatg
     2041 aaatgacgcc tcctcgctca ctcggtcgct gcgctcctgc cgtgagactg cggcgggcgt
     2101 taccagctca cagatcacgg gataagtaag cagcattaaa atcagaagag gccggaaaaa
     2161 ggatgcggcg ttgccgtttt tccataggct ccgcccccct gacaagcagt cacgaaatct
     2221 gacgctcaaa tcagtggcgg cgaaacccga caggactata aagatcccag gcgtttcccc
     2281 ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     2341 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     2401 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttgtccggt
     2461 aactatcgtc ttgagtccaa cccggaaaga cacgacaaag cgccactggc agcagccact
     2521 gataactgat ttagaggagt tagtcttgaa gttatgtgcc cgttaaggct aaactgaaag
     2581 gacagatttg gtgactgtgc tcctccaagc cagttaccgc ggttcaaaga gttgctgctc
     2641 tgagaacctt cgaaaaaacc gccttgctgg gcggtttttt cgtttactga gcaagagaca
     2701 tgacgccaca gaaaggatct caagaagatc cttcaccata atggaaatct atccactgat
     2761 attacaggga tgcatatcgt tataaaacat cggatattat gcatcccaac taaaaatagc
     2821 tcgaggataa aaatgagttc acggtttact gccgaggatg cggcaaggaa atacaatgaa
     2881 acagcaaagt catgcccaca cctgcggagc cacaaacgcc tcttcaggtt caggtgaaaa
     2941 agtagaatcg catgcgctat taggaaggtg cgaacaagcg ggaaattctt cggttgacct
     3001 agtcatttca tttcttcatg tttgagcgat ttttttctcc tgtaaatgcc ttgaatcagc
     3061 ctatttagac cgtttccttc gccatttaag gcgttattcc cagtttttag tgagatctct
     3121 cccactgacg tatcatcggt ccgcccgaaa caggttggcc agcgtgaata acatcgccag
     3181 ttggttatcg tttttcagca acccttgtat ctggctttca cgaagccgaa ctgtcgcttg
     3241 atgatgcgaa atgggtgctc caccttggcc cggatgctgg ctttcatgta accgggcaaa
     3301 cagacgcatg gaggcgattt cgtacagagc atcttcgatc gcgccatcgc tcaggttgta
     3361 ccaatgctgc atgcagtgaa tgcgtagcat ggtttccagc ggataaggtc gccggccatt
     3421 accagccttg gggtaaaacg gctcgatgac ttccaccatg ttttgccatg gcagaatctg
     3481 ctccatgcgg gacaagaaaa tctcttttct gtctgacggc gcttactgct gaattcactg
     3541 tcggcgaagg taagttgatg actcatgatg aaccctgttc catggctcca gatgacaaac
     3601 atgatctcac atcagggact tgttcgcacc ttccctaaat ctgatagtcg gtctacagat
     3661 agatcaatct ggcgttcaga ttattcttac tttcttgtat catccgacca ctgcaaccaa
     3721 tcaggcagaa atttactgcg ctccaaaaag aagaacggtt atgtcagcga tggatttcaa
     3781 gaaaacacgg acctcgattt tccgttatgc cactccggcg gtaagcacca aagagataga
     3841 tctcctgggg ttgttaggac gtacttctgg cagccaaaaa cgtattgtga cgatcgtcct
     3901 ggcgttcgcc gtagtagggt tggccattgc gtttttaatc ccgcaaaatg gaccagtaaa
     3961 gccgtgatca cgccggccga gcagacgcag tggaacacgc tgcgccagat gatggtgacg
     4021 ctgcaggtgc tggacgtcga cgcgaaggtg agccgtggag acgtgtttaa cctgttcatc
     4081 aaagtttcag tcgcagtcgc tgctcgaaga gtacatgaaa acctcccgta cgtgatgtct
     4141 cagctggaag gcaccgaggt agacccgctg gagctgcatc gcgcggtggt gaatattgct
     4201 gagaaaatga aagcgacaga taacacgcag gttaaagacg cagacaaagc gccgtatacc
     4261 tcatggacgc tgagctttac cgccccgacg gcggagacgc gcagacggtt ctggaaggtt
     4321 acatcaacta tatctcccgc attgttgagc aggagacgat ggaaaatatc cgtaaccaga
     4381 tcacgctgaa aacgaaagtc gttcagcagc agctggagct ggatcgcgtg cgtctgacca
     4441 atatccacaa caccaacctg cagcgcctca actattcgct ggaagtggcg aacgcggcag
     4501 gcattaagaa gccggtttta cagcaacgga caggcggtaa agacgatcct gactactccg
     4561 tggcgctggg tgctgacggt attgcg
//
