LOCUS       AP027491                3692 bp    DNA     circular BCT 03-NOV-2023
DEFINITION  Escherichia coli LR-21-1 plasmid pLR-21-1_4 DNA, complete sequence.
ACCESSION   AP027491
VERSION     AP027491.1
DBLINK      BioProject: PRJDB14867
            BioSample: SAMD00562501
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8
            Genome Coverage       :: 199x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-21-1"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-21-1_4"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            940..1446
                     /locus_tag="VEE05_50630"
     CDS             940..1446
                     /locus_tag="VEE05_50630"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEA19345.1"
                     /translation="MSVSLTLQERLKQQHLAESETIQSLTKQQLSSLRSELNGIFQQEL
                     NTMLEDMRRQLHDTKEDMRQSRQSMLLTVVKNRVIFPALSAVTILAVIFLSGWGLSTFQ
                     ESQITANWQQINSQKATLAALESKTAGLNIVQDKNGTFVVLPKGAKVDDSGWTVGKQQA
                     LRIVR"
                     /name="hypothetical protein"
     gene            1450..1683
                     /locus_tag="VEE05_50640"
     CDS             1450..1683
                     /locus_tag="VEE05_50640"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEA19346.1"
                     /translation="MTELETHLLNALKRMEQQFSEQLKASEKAQAALQSMFERTAEDNA
                     TLLKQVSFLDNRVTVLSEQLKQFGNLYRQNRR"
                     /name="hypothetical protein"
     gene            complement(1911..2237)
                     /locus_tag="VEE05_50650"
     CDS             complement(1911..2237)
                     /locus_tag="VEE05_50650"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BEA19347.1"
                     /translation="MNQDALKILAKTLTGEQFKVLMMLLADLDYENYIQIAQADIAEAL
                     GMQKTNVSRSVKALIDLSVILQGPKVGRSYSYRLNPQFGWKGTVRNHKKALTNGLSVIQ
                     GGRS"
                     /name="hypothetical protein"
     Typing          205..1778
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.381"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccgagctgg ttcgcgatca tctcggcagg gtcagcgtgc gaaacaaaga cgtcgatcgg
       61 gaacggattg ccatgcttaa ccgcatcaat gccaacctga acatgattgc ccgctgggtt
      121 aatacgcata aatcggcggc ttcctcggtt gaggtcgttg ctcacctgat ggatatcgag
      181 cgccatattc gggagctgtc gcaatgattg tgggtttcag caggcacggc actggcggtg
      241 gcagtgggcc ggtcggctat gtcaccgata aaacgcggcc cggtcgggaa gattcaccgc
      301 cggaagtcct gaggggatcc ccggaggcta cccgcaacct cattgactcg ctcgatttcc
      361 ggcacaaata cacttctggc gtgctgtctt tcgccccgga ggagaaggtc acgccagaaa
      421 tggagcagta tttcatggat cgctttgaac aggtcgcgtt cgccgggctt gaagccgatc
      481 agtacaacat cctctgggtc aggcataccc atgccggaca tcacgagctg cattttgtga
      541 cgccccgcgt tgagcttagc accgggaaaa gcctgaacat ccgcccgccc ggagatgccg
      601 caaaacaggc tttcgatgac ctgcgcagtg aaattaacgc caggtatggc ctggctgacc
      661 cggatgaccc acggcgcgca cgtgagatcg ctcagcctga tcatgagctg aaaatcgccg
      721 cagaagccct cagaaggggc gagaagccgc aggaagacgt caggctactc atagacgcgg
      781 ttttgtctca gagggccgca caggggctta tacgcagtcg tagcgacgtt atcgagcagg
      841 tttcagacct tggaatgcag gtaacgcgcg aaggtaaaaa ttacatcacc gtgtcagatc
      901 cgcagagcgg ccagcggtgg cgcatgaaag ggggacttta tgagcgtgag tttgacgctg
      961 caggagcgat tgaagcagca gcatctcgca gagagcgaga ctattcaaag cctgacgaag
     1021 cagcagctga gcagtttgcg aagcgagttg aacggcatat ttcagcaaga gctgaatacc
     1081 atgctggaag atatgcgccg gcagctgcac gacacgaaag aggatatgcg ccagagccgg
     1141 cagagcatgt tgctgaccgt cgtgaaaaac cgggtgatat tcccggccct gagcgcggtt
     1201 acgatcttgg ccgttatctt tctgagcggc tggggcctga gtacgtttca ggaaagccag
     1261 atcacggcca actggcagca gatcaacagc cagaaagcca cgctggcggc gctggagagc
     1321 aaaacagcgg gcctgaacat cgttcaggac aagaacggga cgttcgtcgt gctgcccaaa
     1381 ggggcgaaag tggacgacag cggctggaca gtcgggaagc agcaagctct gcggatcgtg
     1441 aggtaaacca tgacggaact ggaaacgcac ttattgaacg ccttgaagcg tatggagcag
     1501 caattcagcg agcagctgaa agcttccgag aaggcgcagg ccgccttgca gagcatgttc
     1561 gagcgtactg cggaagacaa cgccacgctg ttgaagcaag tcagcttctt agacaatcgg
     1621 gtgactgtat tgtcggagca gctgaagcag ttcgggaacc tctacaggca gaacaggcgt
     1681 tgaggaagca gctggaattt caggaagcgt tgcagcagaa acagcgtgaa ccggagcctc
     1741 cggcgccgaa gccaaacagg tattacgggt caagcatgta gagatggcgg gtaaaaaaaa
     1801 accgcgcttg gcggggaaaa tacgttacag aggaatatct ctgtaacgta ttggacaaca
     1861 taataatatt ttagttcaaa gagcccatag ttacgtttca aggtaactat tcagctacgt
     1921 ccgccctgaa tcacgctcag gccatttgtc agggcctttt tgtggttcct tacagtgcct
     1981 ttccatccaa actgaggatt caggcggtaa ctataactgc gccctacttt agggccctga
     2041 aggatcacag acaggtcgat gagagctttc actgaacggc tcacattggt tttctgcatg
     2101 cctaaagctt cggcaatatc agcttgggca atttgaatgt aattttcata atcgagatca
     2161 gctaacagca tcatcaagac tttgaattgc tcccctgtaa gggttttagc aaggatcttt
     2221 aacgcgtctt ggttcatagt gaaatgcctc tcaaaagctg acttctgttt gggtcggata
     2281 actgcgacaa aactacctac ctcttcccct gtagcagtgt ctatctgagt aattctttta
     2341 ctcatttaag aggctctccc aaaaaatagt tatcaataat gataacaacg ttatcaataa
     2401 tgataactat cgtcaagcga gaaccgcgcc acacaagggc tgagagaagc cctccttctg
     2461 tatcttatag cgagaggctt agatggtcgg gtagaccccc tgttttcatc gaatagtgca
     2521 taagcatttc tatgcccgtt caggtgatat tccgcttcct cgttccgact gttcgttcct
     2581 cgctacgctg cggtacgcct cagccggaac ttgcggtgag cggttactgt aattaaatcg
     2641 ttgatgtatc tgatggcttt tttaactgga gagaccaaat gaaacttctg gataggcagt
     2701 gtgagcagct tggaatagct ttgtttgata tatgttttcc ctactctgga aagccagaat
     2761 tggctctgct ttcaaaatat agaaatgatg gttttttagg ggcctcttgc gaaggtggag
     2821 caatcttaac tcttataaaa tcatgcatgt tagacggctt agccagatac aactcctttg
     2881 gtgatcggga tgacgcagta aatcgctatt tagaagcaca gatgacaatt ttatctgata
     2941 aaaaagaaga gcttatagac ataattagtc atgttcctga aaatacgatt tttgataatt
     3001 acgatgaaat aatttctagc cctttcatac aaagcattta tcccactctc tccagagaac
     3061 taggcatggc aatgttctat gccattggcc gtgaaaaaat atcagaaata gctgctgcat
     3121 tttctgcaga cccttaccaa tatcgtagtg gatggccaga tctcacttta gtctatgaaa
     3181 attgtattga gtttgtagaa gtaaagacta ctgataatct tcataaaacc cagttaacaa
     3241 ccatcccaat gattctacaa attctcccag gtacggtttc tgtttgccgt ctaaaaaatc
     3301 gtactgccgc tgaaatcgtc aaacagtaaa tcgtgggggc ccctttgggg ttccgattta
     3361 ctgtttgatg acacccccat agatatcctt accagaggtg gatggtttca cgaagtgagg
     3421 ccatccacct gatagacgcc gttgccctgg gcgagggagc aaagcgaccg ctgcaaggga
     3481 ggggtcaagg ggaggcgaag tctcccattg caaggcccgg tgtgcgacat tgagcgtagc
     3541 gaaatgtagc aacatcgggt agccttgttt aaagtgcaat atgcgacgat ggaggtggaa
     3601 tggaaaagcg cgataacatg ctgcgggtca ggttttctga cgcggaattt gaggcgctga
     3661 agcagctggc cgaagatgca ggctgcacca tg
//
