LOCUS       AP027238                8744 bp    DNA     circular BCT 14-MAR-2024
DEFINITION  Enterococcus faecium JARB-OU2402 plasmid pJAOU2402_5 DNA, complete
            sequence.
ACCESSION   AP027238
VERSION     AP027238.1
DBLINK      BioProject: PRJDB14951
            BioSample: SAMD00565616
            Sequence Read Archive: DRR428002, DRR428058
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Segawa,T., Masuda,K., Hisatsune,J., Kuroki,K. and Sugai,M.
  TITLE     Complete genome sequence of vanA-positive Enterococcus faecium
            strain JARB-OU2402
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Sugai,M. and Segawa,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Takaya Segawa National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center;
            4-2-1, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Flye v. 2.9.1
            Genome Coverage       :: 49x
            Sequencing Technology :: Illumina MiSeq; Nanopore GridION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JARB-OU2402"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJAOU2402_5"
                     /geo_loc_name="Japan:Hiroshima"
                     /collection_date="2021-03-14"
     gene            945..1913
                     /locus_tag="JARBOU2402_30280"
     CDS             945..1913
                     /locus_tag="JARBOU2402_30280"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDW45790.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVGK
                     LNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQE
                     GKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPV
                     SFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRG
                     KATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLL
                     KRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
                     /name="replication initiation protein"
     gene            1916..2149
                     /locus_tag="JARBOU2402_30290"
     CDS             1916..2149
                     /locus_tag="JARBOU2402_30290"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45791.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            2142..2561
                     /locus_tag="JARBOU2402_30300"
     CDS             2142..2561
                     /locus_tag="JARBOU2402_30300"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45792.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            2570..2878
                     /locus_tag="JARBOU2402_30310"
     CDS             2570..2878
                     /locus_tag="JARBOU2402_30310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45793.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            2882..3244
                     /locus_tag="JARBOU2402_30320"
     CDS             2882..3244
                     /locus_tag="JARBOU2402_30320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45794.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            3467..4873
                     /locus_tag="JARBOU2402_30330"
     CDS             3467..4873
                     /locus_tag="JARBOU2402_30330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45795.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSENEIEW"
                     /name="hypothetical protein"
     gene            5318..6271
                     /locus_tag="JARBOU2402_30340"
     CDS             5318..6271
                     /locus_tag="JARBOU2402_30340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDW45796.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVGK
                     LNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQE
                     GKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPV
                     SFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRG
                     KATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLL
                     KRESQNDELTNHLRETFEESADDLKKNWILGYRDLM"
                     /name="replication initiation protein"
     gene            6380..6520
                     /locus_tag="JARBOU2402_30350"
     CDS             6380..6520
                     /locus_tag="JARBOU2402_30350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45797.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGEDN
                     D"
                     /name="hypothetical protein"
     gene            6513..6932
                     /locus_tag="JARBOU2402_30360"
     CDS             6513..6932
                     /locus_tag="JARBOU2402_30360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45798.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            6941..7249
                     /locus_tag="JARBOU2402_30370"
     CDS             6941..7249
                     /locus_tag="JARBOU2402_30370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45799.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            7253..7615
                     /locus_tag="JARBOU2402_30380"
     CDS             7253..7615
                     /locus_tag="JARBOU2402_30380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45800.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     Typing          1037..1900
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.264"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          5410..6272
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.034"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          4..497
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.788"
                     /coverage="100.0"
                     /evalue="9.09e-89"
     Typing          3468..4870
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.094"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7764..8689
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.764"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aagagatggc ggtcattttc caaaaagata tggttccttt agaaagtttc aagaggcttc
       61 ctgatggttc gtatcattta tctgggaatg attttgttga tttgtatcaa cgagctacga
      121 gagcaacaaa tgccaagaga aatgctgata acatgagtga gcaatattta cagatgcttg
      181 agcaaaatac agaattaaat gagtatgtag ctacattgga agaagagcgt catttgtctg
      241 gtagaaaaca attagcagat ttacaagtgg aaaataaaaa gctgaaagaa gaaaatcgct
      301 ctttacgcca gcgtttagag cgattaaaaa acgcatttca aaaatcgatc actcgtttgt
      361 ctatccgttt tggtcttgca gaaaaggata tggggctttc tgaggaatta gaagtattag
      421 agcaggagaa aaccttattt tatcgtcaaa acgtctcaca gcataaatta gagcgttctg
      481 agaatgagat tgagtggtag ccacgatgtg cagtcaacgg taccggaggc ggtttgtgcc
      541 gaggataagg agttgacggc taaaccacta gcccctgaag ctgacatttg ctaaaaaatg
      601 gcaagtccat tttttggcca acttagcgtt agcgaagttt gggggcgccc ttttcaaagg
      661 gtgcggtttt atttttttgg tatttgcata gctaaagaga agtgctatta ttgagttagc
      721 aaacaaatga actggaaaaa gagcaaaaaa ataagtcgag caacgctacc aacgttctcg
      781 acctttacgg aaactaacca cactagttcc gtaactcaaa ttcattatac gaatattttt
      841 aggtttcttc aagactagag attgatggtt atgaattttt agagttacgt gaactctgga
      901 aattctttcc aaaaatctct tttttctttg aggaggaact gaatatgagt aaaaaaagaa
      961 caatcgtgaa tgagtgggaa aaattaacag ccctgtctaa caggcggtta aatgttcaaa
     1021 ctttggtaca aactataaaa ttaaattggt cgattgaccg aattaccatt gttggaaagt
     1081 tgaatcaaaa tattttttat catactcaac atgatgtatt aatattagat tttgaacagc
     1141 ttatgcgggt aaatgaaggc aatggctatt taaaagcggt tggaaataat ggttggcagc
     1201 tattagacca acatgacgaa aatattgcct atattgaaat ttctaaatgg caagaaggaa
     1261 aagggcggat tgattttaat cctaataaaa tcagtcgatt tttagcggat tccatgaaaa
     1321 attttattca tgatttattt ttagagcccc atttttcacg agcggatgtt gcctgcgatc
     1381 ttattgatgt gcctgatgag tttatcacgc aatatcgaat tattgatcca gtttcattta
     1441 aattaattta tggacgctca ggaaaactgg aaacggtgta ttggggtagt cgggcaagcg
     1501 agcggcaaat tcgtttgtac aataaaaaaa tagagcaaga aatgaaaaga aaggtggtgc
     1561 cttcagaaat taatacttgg tggcggttag aaatgcagtt aagacgtggt aaagctacgg
     1621 aatggtatgc tatggtgaaa gaaagtttgg atagctttgt gagtccacat tttttaccaa
     1681 ctgatataaa acctacggat aaagttatga ttgatggatt gttagctaat cattctaatt
     1741 gggcgatttt gtcaagaaac tcgaaatata aatatcgaaa tttattaaag cgagaaagtc
     1801 aaaatgatga gttgacgaat catttacgag aaacatttga agaatcagca gatgatttaa
     1861 aaaaagaatt ggatacttgg ttacagggac ttgatgtgac agaggaggaa tagtaatggc
     1921 agaaaaacaa aagcattttg gtaagaaaaa ttggttggtg aatttaacag gtgataacgt
     1981 tgtcgttgtt tttttggata gcaaaaaaat gtccggtaag ttacttgatg taacgcagta
     2041 tgagttgatt ttgaaaaata ataaaattga ttccccaatc attatcccta agcatgctgt
     2101 taaatatgtt tatggggtag ctgaaagtaa cggagaagat aatgactgat ttgccagaaa
     2161 aaatgaccat aaaaaacctt gctgatgagt tgggtatatc taaaaataca gtgcgcaatc
     2221 aactggtcaa tgcaggttat gaccttgctc ggtataaaga taatggggtt atcatgttag
     2281 acaaggaatt gattggtgtt gtgcggtcaa tttatcggtc aaaaggtggt caattgactg
     2341 gtgatttacc ggtcaatgat gcaggcaatg atttcccgtt agaaaaagaa aatgaatttt
     2401 tgcgtgaaca attagcggaa aaagataggc atatttctga attaacggaa atgattcgtg
     2461 accaacaaaa attgactttg cttcagcaac aaaaaaacga tcagttgtta ctagaaaatc
     2521 aagaattaaa aaatcgaaaa tggtggcaat tctggaagtg aggtgtgaca tggataaaat
     2581 tgatttgaga gaagaaacag tcgatagaat tgaattgatg tatgaacggt tagctgatta
     2641 tttgaatttg ccggatgaag ataattatag tgaaatgctt gaacgaatta tggatgttct
     2701 caactcggtt atcaatgctg atctagcaaa atcttcagtt ttgactaatg aatttgttca
     2761 taagcattta gcgcaaacaa ctgatgaatc agaaaaagaa ttttggttga aattaatgag
     2821 tcatgttatg cagttaaacc aaatttccgt tattaattcc aaagaaaagg agatgtagtt
     2881 tatgggaatg actttagcag atattaaagc gcaagaagaa aaattaaaaa aagaacaagc
     2941 gaaacttcgc caacagaaaa aacgcttgat tgagaaagaa aatgctgcaa tgggcaaggc
     3001 tcttcgcaag cgttttgggt ttgaaacttt tgctgatttt gagcggtggt tatcgtcaat
     3061 ttctgacgat cagcaacacg aaaaaacagt ggataatgag tggcttgctg atgaaaaatc
     3121 agcgccaagc gaacgagatt atcccgatga atctgacgac ttattcggag tgataccgaa
     3181 taagaaaagt cagatgagtg agagtaaatt tacgtcaata tttaatgacg tgaatttacg
     3241 ataagcgatt attttgcgtg aagcaaaata aagcataaaa ggagttgata gcgtgatacg
     3301 ctttcggctc cttttttcgt tgtcacttgt gacagcgaaa accctcggag agcgtttgga
     3361 attgtgacat ccatgtcaca attaggaaaa tgtttactct ttgacaaaga gtaacagaaa
     3421 ggtttacaat ctttttatta gcgacagcgc gaaggagggt tttaacttgg ggtttagtat
     3481 ttcatttaaa aaaggaacga agaatacaag tatttctcat aataatcgtg agttaactga
     3541 tcgccagaaa accaatgatt ggcataagca cattgacttc acgaaatcgg atgaaaatat
     3601 ttatttagag cagacgaatc tagcagataa atatgaggaa ttgtttggcg aagtggtaga
     3661 aaaatataat gcgaaacaaa aaagagccga tagaaaaatc gaggactatt tagcgaaagt
     3721 gcgcaaggat aaaaagttgg aacctcaacg agaatttatt gtgcaaatag gcattttaga
     3781 tgatttccag acaaatcgag cagatggttc ttctactggc atatcgaaac aacaagctga
     3841 acaaaatcga gtgattgcca ataaaatttt ggttcagtat tacaaggagt ttcaagagag
     3901 aaaccctaat ttggctgttt ataatgctgt tattcataat gacgaaataa gtccgcacct
     3961 gcacttgaat attgtgccgg ttgctgaagg ttataaacgg ggtgttcaaa agcagccgag
     4021 ctttaataaa gcactggttc aacaagggat acacgctgat aaagataatg gtcgagtgct
     4081 tttcaataat tttagaaatc aagaagttga ctctatagaa cgattaatga atgattacgg
     4141 ttggaaaagg gatattgtcg gtaccaacaa aattaaagat attcacgaat acaaagaaat
     4201 catgagtgaa atttctgaat tgcgccaaac ggctgtaaaa gagcgtgaga gcgtttctga
     4261 tgagttgagg tatatttata ggcaaagaga agaaaacgag cgtgataagg ctctgatggc
     4321 ttctgagaga gcgaagattg aagaagagaa gaaaaatatg ctgaatgtaa aaaaagagat
     4381 ggcggtcatt ttccaaaaag atatggttcc tttagaaagt ttcaagaggc ttcctgatgg
     4441 ttcgtatcat ttatctggga atgattttgt tgatttgtat caacgagcta cgagagcaac
     4501 aaatgccaag agaaatgctg ataacatgag tgagcaatat ttacagatgc ttgagcaaaa
     4561 tacagaatta aatgagtatg tagctacatt ggaagaagag cgtcatttgt ctggtagaaa
     4621 acaattagca gatttacaag tggaaaataa aaagctgaaa gaagaaaatc gctctttacg
     4681 ccagcgttta gagcgattaa aaaacgcatt tcaaaaatcg atcactcgtt tgtctatccg
     4741 ttttggtctt gcagaaaagg atatggggct ttctgaggaa ttagaagtat tagagcagga
     4801 gaaaacctta ttttatcgtc aaaacgtctc acagcataaa ttagagcgtt ctgagaatga
     4861 gattgagtgg tagccacgat gtgcagtcaa cggtaccgga ggcggtttgt gccgaggata
     4921 aggagttgac ggctaaacca ctagcccctg aagctgacat ttgctaaaaa atggcaagtc
     4981 cattttttgg ccaacttagc gttagcgaag tttgggggcg cccttttcaa agggtgcggt
     5041 tttatttttt tggtatttgc atagctaaag agaagtgcta ttattgagtt agcaaacaaa
     5101 tgaactggaa aaagagcaaa aaaaataagt cgagcaacgc taccaacgtt ctcgaccttt
     5161 acggaaacta accacactag ttccgtaact caggttcatt atacgaatat ttttaggttt
     5221 cttcaagcta gagatttttg gatatgaatt tttagggtta cgtgaactct ggaaattctt
     5281 tccaaaagtc tcttttttct ttgaggagga actgaatatg agtaaaaaaa gaacaatcgt
     5341 gaatgagtgg gaaaaattaa cagccctgtc taacaggcgg ttaaatgttc aaactttggt
     5401 acaaactata aaattaaatt ggtcgattga ccgaattacc attgttggaa agttgaatca
     5461 aaatattttt tatcatactc aacatgatgt attaatatta gattttgaac agcttatgcg
     5521 ggtaaatgaa ggcaatggct atttaaaagc ggttggaaat aatggttggc agctattaga
     5581 ccaacatgac gaaaatattg cctatattga aatttctaaa tggcaagaag gaaaagggcg
     5641 gattgatttt aatcctaata aaatcagtcg atttttagcg gattccatga aaaattttat
     5701 tcatgattta tttttagagc cccatttttc acgagcggat gttgcctgcg atcttattga
     5761 tgtgcctgat gagtttatca cgcaatatcg aattattgat ccagtttcat ttaaattaat
     5821 ttatggacgc tcaggaaaac tggaaacggt gtattggggt agtcgggcaa gcgagcggca
     5881 aattcgtttg tacaataaaa aaatagagca agaaatgaaa agaaaggtgg tgccttcaga
     5941 aattaatact tggtggcggt tagaaatgca gttaagacgt ggtaaagcta cggaatggta
     6001 tgctatggtg aaagaaagtt tggatagctt tgtgagtcca cattttttac caactgatat
     6061 aaaacctacg gataaagtta tgattgatgg attgttagct aatcattcta attgggcgat
     6121 tttgtcaaga aactcgaaat ataaatatcg aaatttatta aagcgagaaa gccaaaacga
     6181 tgagttgacg aatcatttac gagaaacatt tgaagaatca gcagatgatt taaaaaagaa
     6241 ttggatactt ggttacaggg acttgatgtg acagaggagg aatagtaatg gcagaaaaac
     6301 aaaagcattt tggtaagaaa aattggttgg tgaatttaac aggtgataac gttgtcgttg
     6361 ttttttggat agcaaaaaaa tgtccggtaa gttacttgat gtaacgcagt atgagttgat
     6421 tttgaaaaat aataaaattg attccccaat cattatccct aagcatgctg ttaaatatgt
     6481 ttatggggta gctgaaagta acggagaaga taatgactga tttgccagaa aaaatgacca
     6541 taaaaaacct tgctgatgag ttgggtatat ctaaaaatac agtgcgcaat caactggtca
     6601 atgcaggtta tgaccttgct cggtataaag ataatggggt tatcatgtta gacaaggaat
     6661 tgattggtgt tgtgcggtca atttatcggt caaaaggtgg tcaattgact ggtgatttac
     6721 cggtcaatga tgcaggcaat gatttcccgt tagaaaaaga aaatgaattt ttgcgtgaac
     6781 aattagcgga aaaagatagg catatttctg aattaacgga aatgattcgt gaccaacaaa
     6841 aattgacttt gcttcagcaa caaaaaaacg atcagttgtt actagaaaat caagaattaa
     6901 aaaatcgaaa atggtggcaa ttctggaagt gaggtgtgac atggataaaa ttgatttgag
     6961 agaagaaaca gtcgatagaa ttgaattgat gtatgaacgg ttagctgatt atttgaattt
     7021 gccggatgaa gataattata gtgaaatgct tgaacgaatt atggatgttc tcaactcggt
     7081 tatcaatgct gatctagcaa aatcttcagt tttgactaat gaatttgttc ataagcattt
     7141 agcgcaaaca actgatgaat cagaaaaaga attttggttg aaattaatga gtcatgttat
     7201 gcagttaaac caaatttccg ttattaattc caaagaaaag gagatgtagt ttatgggaat
     7261 gactttagca gatattaaag cgcaagaaga aaaattaaaa aaagaacaag cgaaacttcg
     7321 ccaacagaaa aaacgcttga ttgagaaaga aaatgctgca atgggcaagg ctcttcgcaa
     7381 gcgttttggg tttgaaactt ttgctgattt tgagcggtgg ttatcgtcaa tttctgacga
     7441 tcagcaacac gaaaaaacag tggataatga gtggcttgct gatgaaaaat cagcgccaag
     7501 cgaacgagat tatcccgatg aatctgacga cttattcgga gtgataccga ataagaaaag
     7561 tcagatgagt gagagtaaat ttacgtcaat atttaatgac gtgaatttac gataagcgat
     7621 tattttgcgt gaagcaaaat aaagcataaa aggagttgat agcgtgatac gctttcggct
     7681 ccttttttcg ttgtcacttg tgacagcgaa aaccctcgga gagcgtttgg aattgtgaca
     7741 tccatgtcac aattaggaaa atgtttactc tttgacaaag agtaacagaa aggtttacaa
     7801 tctttttatt agcgacagcg cgaaggaggg ttttaacttg gggtttagta tttcatttaa
     7861 aaaaggaacg aagaatacaa gtatttctca taataatcgt gagttaactg atcgccagaa
     7921 aaccaatgat tggcataagc acattgactt cacgaaatcg gatgaaaata tttatttaga
     7981 gcagacgaat ctagcagata aatatgagga attgtttggc gaagtggtag aaaaatataa
     8041 tgcgaaacaa aaaagagccg atagaaaaat cgaggactat ttagcgaaag tgcgcaagga
     8101 taaaaagttg gaacctcaac gagaatttat tgtgcaaata ggcattttag atgatttcca
     8161 gacaaatcga gcagatggtt cttctactgg catatcgaaa caacaagctg aacaaaatcg
     8221 agtgattgcc aataaaattt tggttcagta ttacaaggag tttcaagaga gaaaccctaa
     8281 tttggctgtt tataatgctg ttattcataa tgacgaaata agtccgcacc tgcacttgaa
     8341 tattgtgccg gttgctgaag gttataaacg gggtgttcaa aagcagccga gctttaataa
     8401 agcactggtt caacaaggga tacacgctga taaagataat ggtcgagtgc ttttcaataa
     8461 ttttagaaat caagaagttg actctataga acgattaatg aatgattacg gttggaaaag
     8521 ggatattgtc ggtaccaaca aaattaaaga tattcacgaa tacaaagaaa tcatgagtga
     8581 aatttctgaa ttgcgccaaa cggctgtaaa agagcgtgag agcgtttctg atgagttgag
     8641 gtatatttat aggcaaagag aagaaaacga gcgtgataag gctctgatgg cttctgagag
     8701 agcgaagatt gaagaagaga agaaaaatat gctgaatgta aaaa
//
