LOCUS       AP027236               12368 bp    DNA     circular BCT 14-MAR-2024
DEFINITION  Enterococcus faecium JARB-OU2402 plasmid pJAOU2402_3 DNA, complete
            sequence.
ACCESSION   AP027236
VERSION     AP027236.1
DBLINK      BioProject: PRJDB14951
            BioSample: SAMD00565616
            Sequence Read Archive: DRR428002, DRR428058
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Segawa,T., Masuda,K., Hisatsune,J., Kuroki,K. and Sugai,M.
  TITLE     Complete genome sequence of vanA-positive Enterococcus faecium
            strain JARB-OU2402
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12368)
  AUTHORS   Sugai,M. and Segawa,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Takaya Segawa National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center;
            4-2-1, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Flye v. 2.9.1
            Genome Coverage       :: 49x
            Sequencing Technology :: Illumina MiSeq; Nanopore GridION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..12368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JARB-OU2402"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJAOU2402_3"
                     /geo_loc_name="Japan:Hiroshima"
                     /collection_date="2021-03-14"
     gene            complement(194..364)
                     /locus_tag="JARBOU2402_30010"
     CDS             complement(194..364)
                     /locus_tag="JARBOU2402_30010"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45764.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            complement(379..912)
                     /locus_tag="JARBOU2402_30020"
     CDS             complement(379..912)
                     /locus_tag="JARBOU2402_30020"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDW45765.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(933..1877)
                     /gene="rep_1"
                     /locus_tag="JARBOU2402_30030"
     CDS             complement(933..1877)
                     /gene="rep_1"
                     /locus_tag="JARBOU2402_30030"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDW45766.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="rep_1"
     gene            complement(2721..3410)
                     /locus_tag="JARBOU2402_30040"
     CDS             complement(2721..3410)
                     /locus_tag="JARBOU2402_30040"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45767.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3417..3707)
                     /locus_tag="JARBOU2402_30050"
     CDS             complement(3417..3707)
                     /locus_tag="JARBOU2402_30050"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748752.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45768.1"
                     /translation="MTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTT
                     IDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(3655..4569)
                     /gene="mobA"
                     /locus_tag="JARBOU2402_30060"
     CDS             complement(3655..4569)
                     /gene="mobA"
                     /locus_tag="JARBOU2402_30060"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="BDW45769.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="mobA"
     gene            complement(4551..4940)
                     /gene="mobC_1"
                     /locus_tag="JARBOU2402_30070"
     CDS             complement(4551..4940)
                     /gene="mobC_1"
                     /locus_tag="JARBOU2402_30070"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDW45770.1"
                     /translation="MGLMSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_1"
     gene            complement(5379..5666)
                     /locus_tag="JARBOU2402_30080"
     CDS             complement(5379..5666)
                     /locus_tag="JARBOU2402_30080"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45771.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            complement(5686..5910)
                     /locus_tag="JARBOU2402_30090"
     CDS             complement(5686..5910)
                     /locus_tag="JARBOU2402_30090"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45772.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     gene            complement(6564..7046)
                     /locus_tag="JARBOU2402_30100"
     CDS             complement(6564..7046)
                     /locus_tag="JARBOU2402_30100"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDW45773.1"
                     /translation="MSRQAISKHIQKLDSSMIAKNERGYKVVLRSGVLQLARNLDNQRL
                     LDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQLALKEQNNSHIQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMDEQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(7139..8059)
                     /gene="rep_2"
                     /locus_tag="JARBOU2402_30110"
     CDS             complement(7139..8059)
                     /gene="rep_2"
                     /locus_tag="JARBOU2402_30110"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDW45774.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKLLKNKQSPHFRKKRKKFII"
                     /name="rep_2"
     gene            complement(8903..9592)
                     /locus_tag="JARBOU2402_30120"
     CDS             complement(8903..9592)
                     /locus_tag="JARBOU2402_30120"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45775.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(9599..10045)
                     /locus_tag="JARBOU2402_30130"
     CDS             complement(9599..10045)
                     /locus_tag="JARBOU2402_30130"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45776.1"
                     /translation="MSWTDENEPTINNEPTSYEELELAFAKLQKKYEENNELLRLNNRK
                     LDRLEETMTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQVKK
                     FHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(10142..10750)
                     /locus_tag="JARBOU2402_30140"
     CDS             complement(10142..10750)
                     /locus_tag="JARBOU2402_30140"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 10201"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45777.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKLERITNFVILLKHSAFIA"
                     /name="hypothetical protein"
     gene            complement(10732..11121)
                     /gene="mobC_2"
                     /locus_tag="JARBOU2402_30150"
     CDS             complement(10732..11121)
                     /gene="mobC_2"
                     /locus_tag="JARBOU2402_30150"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDW45778.1"
                     /translation="MGLMSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_2"
     gene            complement(11562..11849)
                     /locus_tag="JARBOU2402_30160"
     CDS             complement(11562..11849)
                     /locus_tag="JARBOU2402_30160"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45779.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            complement(11869..12093)
                     /locus_tag="JARBOU2402_30170"
     CDS             complement(11869..12093)
                     /locus_tag="JARBOU2402_30170"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW45780.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     BGC             686..12368
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(934..1877)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7118..8059)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.788"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3659..4569)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10164..10750)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.837"
                     /coverage="100.0"
                     /evalue="2.37e-118"
ORIGIN
        1 ttaaggcttg gttttctgcg gttgtttctt tctaattctt tgtgaatatt gatcggtcat
       61 tttttaaata cttgttattt ttgtttgtgt ttgttatgct ttttacatat ctcatattca
      121 tttattattc ttttctaagg ttaataatag gataatctct ttcagaaaga ctgctcgtac
      181 gagcagtctt ttcttattta tcaagttttt ctacaaatac ttcctcattt tgactgttgt
      241 agtaattagg attgagtttt agttcccctt ttttatttcc tttgttggcc actgataata
      301 acattgggct aaagcttaat attcctagta ggatagaacc gaatagcaag tgtttttttg
      361 tcataagtat ttccttcttc atttccatag ctgccaccat tttttccaat tctttgtttg
      421 ttcgtccatt gtttctgttg cttcagaaag agtcgttgaa ctattctctg aagactcttc
      481 cgtataagtc aactgcagtt gctcctgaag tttttcgatt tgttggttgg cttgcagggt
      541 gagttgttgt tgctggtcga gtagtttttg tatatgagaa ttattttgct ctttgagggc
      601 aagttgttct cgtaaaaatt cattttcttc acgcaactga tctagcaact tgtttgcaac
      661 cacgtttttg tttggtatat aagcattttt atcgttgttt tgttcatgtt tatgcaactt
      721 atctagcaac ctttgattgt ctaagttgcg agcaagttgc aatactccag aacgcaatac
      781 aaccttataa ccacgctcat ttttagctat catggaagaa tctaattttt ggatatgttt
      841 cgatattgct tgcctactca tcttaaaaag atctgacaat tcctttattg taaaggtttc
      901 ttgtggttgc atactggttg cctcctaggt tgctactgga ataaattatc taaatcagat
      961 aataaacttt ttttctcttt ttccgaaagt gcggactctg tttgtttttc aagagctttt
     1021 ttctcttttt ctagttgttt gatttgttgg aggagttcaa gttctcgttt cttctgtttg
     1081 attttttcgt taattgttgt agcgggttct tcgtattctt tttgtttatc agctaaagcc
     1141 aatgctttat tttggtcttc tttggttggt gcatgtttgt taaagtcgtc tgcttttttg
     1201 atctcaggtt tgaacgtaaa ttgatagcca ataacaggtt ttccccgtcc ttttccgtat
     1261 ttctttttga ttgttagtcc tttgaagatt gcagttagtt cttgacgtat aggttttata
     1321 actcgtttat ctatatcagg ttgcttataa cttttaggaa tatctaataa ttcaagaaag
     1381 tcttctttgg agaaatatgc gaaacctttg gttctgtatt gctttaacag acgaaacata
     1441 gtctttgcat agccactttg tagctcgttg aattgttgta aagaatagcg tacccactcg
     1501 tctaaattat ttaacaaagg cagtgctttt tcgtaaattc gtatatctac gtaaggattt
     1561 tctacgtccc cgttgatttt aaattctgta aacattacaa acatatcgta agaaagccca
     1621 gactcacttc tacgaccaaa gcgtaaagat aagatttttt cataagttgc ttttaaatct
     1681 tctacaaatc gtacatttgc agttgctttg tagttactta aatcttttag ttggtcaaac
     1741 gtaaaacgaa ctgtcttatc ccctttttca cgcattctag acacgatgga aaaaaacaaa
     1801 ttcatttcaa ccggtgtaaa ttttcttaaa ggaattgtat ttaattcatg gtggtatttt
     1861 acaatctcat tagccatata atcacctcaa tccaattatg aatgacattt tttaaatgtc
     1921 aagacattca tatttgacgt tcaaacagac aaaatgtcct tcataaacag acaaaatgtc
     1981 cttcataaac agacaaaatg tccttcataa acagacaaaa tgtccttcat aacattctgt
     2041 atccattggt agagtaagga cacagcttac ggtaaagagg tttaaaaaga ggtttaaaaa
     2101 gagatataaa aagagggggg ctaattttcc cgcctaaaaa ccacttcttt tttctttaaa
     2161 caggttcgcg catttttcaa aactgccaat ccaaaacctt gcttcctacg gtcgccaaaa
     2221 aggtgtgatc gctacgctct cacattgtac tgcgcagtcg cctaaaggct cctttgctaa
     2281 aaacatgttg gtaaagaggc ctttgagcgg ttctgaatgc ttatctaagc cgttttaaga
     2341 cagtcttgga taaaaatcct tatctagctt taaagcgcct tacaggcggt cagggcaagc
     2401 cctaacaacc ctaaaataag cgatacttaa aaacaccaca caagtttaat tttttaagat
     2461 ataataactt gtcgcctatc cgacagtctt gcgactggaa ggaggtgtat ctaatgtttg
     2521 aattgttcat ttgccccctt cttatagaag tttgtgtctc tttattcagt tattggttga
     2581 ataaccgaaa caagaagtaa aatcaataaa ggcgacatca acctgaaaaa aagccccaga
     2641 gaatccgacc tctctggggc tttcgtgtat ctaatgtttg atcatttgca tgtttagttt
     2701 aacatgattg ccgatcaaat tcaattttgc tgttgctgat ctaaccattc cttgacactt
     2761 tttagataat ttaatgccgt ttcttttttc gttggatttt gtatgttcgt tgcttgctga
     2821 agcagtgctt tataacgttc gtaggtaact gattgttgcc gttcttttcg ttcctgttca
     2881 ctagttgaaa aagggattgt atcactgaat aaattataac aagcttcttc tgtctgcgta
     2941 tctttaaaat attgcacgtc ttttttggtt cgctcatagt tgtcttgaac ctctttttgc
     3001 tgtttttctg gcaagttttg ataaagacga gtgagttttt ccatgttttt atccgtcaaa
     3061 cgtttttctt gattcacaaa aatatcgttc atttcctcat aacaagtggt aaactcttcc
     3121 attttttgtg ttaacacttg ctcctttttc tttttatcac atgctgcttc atacttttga
     3181 gtgatatccc ctaataaaat acttcttgaa atacgtttat ttccctctaa aacgccgaat
     3241 aaaccactta aaacgattga taatgtaaat ataacccctg gcaataaaaa cctcttaaat
     3301 cgaattttag agccttttaa tgaatatctt gtttgataag ccttatagct aagccaaata
     3361 attcctaaaa tactggcaaa aatgagtaga tagggtaaat agaatgccat ttctatttat
     3421 tctccccata ccagccaaaa ggctaacagt gtcatgatta agcaacataa gggggcaaga
     3481 atatccattg atcgctgcat aattactttt ttctgttctt tatttcgctg gtttaatagc
     3541 gcatggtgga attttttgac ttgtgaatca attgtagttt gtattctttc tttggtataa
     3601 ggtgcaagaa aagcacgttg tttctttatt tcattttcta tgtcttgtaa cgttttagaa
     3661 cgataattct tgttggtagt ctgctcctct ttcaacgtgt tggtcatggt ttcttcgagt
     3721 cggtctaatt tccggttgtt cagtcgtaag agttcgttgt tctcttcgta cttcttttgc
     3781 agtttcgcga atgctagctc gagctcttcg tagctcgttg gttcgttgtt gattgtaggc
     3841 tcgttctcgt ctgtccagct catgtaaaat cgtctccttt tcgtaatcct ttcctagttt
     3901 gttggatcgt acttttttct ttttgtggat caattcgtaa gtaaaatttt tcccacgatt
     3961 caagcaatga atgccgaatg ttttaagaga atcacaaaat tggttatacg ttcgagtttt
     4021 tttgtttttc attgtttgat caatcgcaaa ccgaatttcg tctttccaaa caggttcatt
     4081 tttttctttt aatttgattt cagacatggt tcgtttttcg ggtttcttcc ctcgttcagt
     4141 gattgttaag tcatgttcaa gacaaatctc gtcatttagg tcataacatt tgtttaaaaa
     4201 tgcttgacca tgggcttgaa atttgttccc agtttccaga ttgacggagt taatgacaat
     4261 gtggttatga atatggtctt tgtctgtatg tgtgtaaatc gcgatttggt ggtcaggggc
     4321 tatttttttc gcatactctg cccctagttg attggctttt tcagcggtaa tttctcctgg
     4381 tcgaaaagac tggatcaata agtgtgcttg tacacgatcg tcttttccat acagcatgcg
     4441 aatttgtttc atttcgcttt tggcataatc aatgtcacag tttatccctg ttttgacggt
     4501 tgctcgtggt tcggcgtaat tgatcgctcg gctacaagaa gtggttcggc ttactttaac
     4561 tgttgccata tcttttgtag ctccttttgc atgattttta ggttttcgga taagcgttgg
     4621 gcgtcgccag gatcaacgtc gtgttggtgt gtgttgcacc acttggcaat ttgattgata
     4681 ttcccgccag ctttggctaa atcgcgcgcg attgcctgag caatgtctgg cgccactttc
     4741 ggtgtcacca gacgcgcgtt ttgtgccttg gctttcacga aagccggcac actcatttgc
     4801 aaagcttcag ctgactgctt cagctttgca aattcggatt cgctcactcg aaaactgatt
     4861 tgttttggct cggatcgata ggtatatttt ttagaatgat tgctagccaa atattgattt
     4921 tgttcgctca taagtcccac cctttcagta cgtcctacgg acgtatcgtt cgcaatacca
     4981 ttgtacctaa aaaggtacaa taagtaaagc tcactaaagc tcactaaagg gcttggcaag
     5041 ctctatattt gctagccaaa tacctacgta tttggtgcaa ataaccgctt gtcaaagggg
     5101 cgcaagctcc ccttgaatcc ccctcccccc actctccaca atacaaaaaa gactagcaaa
     5161 tgctagccac tttttgtaaa gtttcgcttg tttcaccttc ggtgaaattg ttgcaaatca
     5221 ataattaggt agagctacat ttttttcgtt gttcaaagat tcttatgcca tttactatta
     5281 ataaatctat aaatatgata atttttcggc tctataattt atgggactga tgaatcagaa
     5341 ttgattcttc agtcccattt tcattttatt ccaaagaatt aactccaaat accaatagaa
     5401 gcccatcctc tatatacttg agattcctta ctaacaattt cttgtaattc tattaaagat
     5461 ttaggtaatc caaatttatg ttttaaaata tatcttccaa gaaaactact taatttcata
     5521 ctaacttcac caacactttt tccctcactg atttctttag aataaagaaa gagcttttct
     5581 tttaagtcag gatagtcatc tatagttgct tcattgtata ctttactaat tgcatttaaa
     5641 agtttttctt ctttagtaaa atccattata ataacctccc taccactacc ttctaggtgc
     5701 ccatggacca tgattaaccc aaccattaac aataattttt ccaatttctc ctttagcttg
     5761 attccaatct acccaacatt tttctttgtt acaataaaga ccatttccat agtaagtagc
     5821 tgcatcaact tttattcctg taccgatgcc agctagacaa gttcctaata ttcctagaat
     5881 aacacaatgt tttaatactt tctttttcat gattgttttt ccttttcgtt tttttaaaat
     5941 tgcttgttat attaaataat cttagtaatt ttattatata acattttatt atataacaat
     6001 ttattatata acaatgatta acgtcataaa aagtagtttt gaattcataa tcgtttaaat
     6061 aaaattataa gatcagttat ttaaacagct agtagacata atgtgtatac taccaagtaa
     6121 cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt ttattggtct aggttaaaag
     6181 ctctttttaa ggcttggttt tctgcggttg tttctttcta attctttgtg aatattgatc
     6241 ggtcattttt taaatacttg ttatttttgt ttgtgtttgt tatgcttttt acatatctca
     6301 tattcattta ttattctttt ctaaggttaa taataggata atctctttca gaaagactgc
     6361 tcgtacgagc agtcttttct tatttatcaa gtttttctac aaatacttcc tcattttgac
     6421 tgttgtagta attaggattg agttttagtt ccccttttta tttcctttgt tggccactga
     6481 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     6541 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     6601 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     6661 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     6721 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     6781 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     6841 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     6901 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     6961 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     7021 tgtttcgata ttgcttgcct actcatctta aaagatctga caattccttt attgtaaagg
     7081 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     7141 agataataaa cttttttctc tttttccgaa agtgcggact ctgtttgttt ttcaagagct
     7201 ttttctcttt ttctagttgt ttgatttgtt ggaggagttc aagttctcgt ttcttctgtt
     7261 tgattttttc gttaattgtt gtagcgggtt cttcgtattc tttttgttta tcagctaaag
     7321 ccaatgcttt attttggtct tctttggttg gtgcatgttt gttaaagtcg tctgcttttt
     7381 tgatctcagg tttgaacgta aattgatagc caataacagg ttttccccgt ccttttccgt
     7441 atttcttttt gattgttagt cctttgaaga ttgcagttag ttcttgacgt ataggtttta
     7501 taactcgttt atctatatca ggttgcttat aacttttagg aatatctaat aattcaagaa
     7561 agtcttcttt ggagaaatat gcgaaacctt tggttctgta ttgctttaac agacgaaaca
     7621 tagtctttgc atagccactt tgtagctcgt tgaattgttg taaagaatag cgtacccact
     7681 cgtctaaatt atttaacaaa ggcagtgctt tttcgtaaat tcgtatatct acgtaaggat
     7741 tttctacgtc cccgttgatt ttaaattctg taaacattac aaacatatcg taagaaagcc
     7801 cagactcact tctacgacca aagcgtaaag ataagatttt ttcataagtt gcttttaaat
     7861 cttctacaaa tcgtacattt gcagttgctt tgtagttact taaatctttt agttggtcaa
     7921 acgtaaaacg aactgtctta tccccttttt cacgcattct agacacgatg gaaaaaaaca
     7981 aattcatttc aaccggtgta aattttctta aaggaattgt atttaattca tggtggtatt
     8041 ttacaatctc attagccata taatcacctc aatccaatta tgaatgacat tttttaaatg
     8101 tcaagacatt catatttgac gttcaaacag acaaaatgtc cttcataaac agacaaaatg
     8161 tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataacattct
     8221 gtatccattg gtagagtaag gacacagctt acggtaaaga ggtttaaaaa gaggtttaaa
     8281 aagagatata aaaagagggg ggctaatttt cccgcctaaa aaccacttct tttttcttta
     8341 aacaggttcg cgcatttttc aaaactgcca atccaaaacc ttgcttccta cggtcgccaa
     8401 aaaggtgtga tcgctacgct ctcacattgt actgcgcagt cgcctaaagg ctcctttgct
     8461 aaaaacatgt tggtaaagag gcctttgagc ggttctgaat gcttatctaa gccgttttaa
     8521 gacagtcttg gataaaaatc cttatctagc tttaaagcgc cttacaggcg gtcagggcaa
     8581 gccctaacaa ccctaaaata agcgatactt aaaaacacca cacaagttta attttttaag
     8641 atataataac ttgtcgccta tccgacagtc ttgcgactgg aaggaggtgt atctaatgtt
     8701 tgaattgttc atttgccccc ttcttataga agtttgtgtc tctttattca gttattggtt
     8761 gaataaccga aacaagaagt aaaatcaata aaggcgacat caacctgaaa aaaagcccca
     8821 gagaatccga cctctctggg gctttcgtgt atctaatgtt tgatcatttg catgtttagt
     8881 ttaacatgat tgccgatcaa attcaatttt gctgttgctg atctaaccat tccttgacac
     8941 tttttagata atttaatgcc gtttcttttt tcgttggatt ttgtatgttc gttgcttgct
     9001 gaagcagtgc tttataacgt tcgtaggtaa ctgattgttg ccgttctttt cgttcctgtt
     9061 cactagttga aaaagggatt gtatcactga ataaattata acaagcttct tctgtctgcg
     9121 tatctttaaa atattgcacg tcttttttgg ttcgctcata gttgtcttga acctcttttt
     9181 gctgtttttc tggcaagttt tgataaagac gagtgagttt ttccatgttt ttatccgtca
     9241 aacgtttttc ttgattcaca aaaatatcgt tcatttcctc ataacaagtg gtaaactctt
     9301 ccattttttg tgttaacact tgctcctttt tctttttatc acatgctgct tcatactttt
     9361 gagtgatatc ccctaataaa atacttcttg aaatacgttt atttccctct aaaacgccga
     9421 ataaaccact taaaacgatt gataatgtaa atataacccc tggcaataaa aacctcttaa
     9481 atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag ctaagccaaa
     9541 taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc atttctattt
     9601 attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat aagggggcaa
     9661 gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc tggtttaata
     9721 gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt tctttggtat
     9781 aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt aacgttttag
     9841 aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg gtttcttcga
     9901 gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg tacttctttt
     9961 gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg ttgattgtag
    10021 gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc ctttcctagt
    10081 ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt tttcccacga
    10141 ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata cgttcgagtt
    10201 ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa acaggttcat
    10261 ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc cctcgttcag
    10321 tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat ttgtttaaaa
    10381 atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag ttaatgacaa
    10441 tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg tggtcagggg
    10501 ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta atttctcctg
    10561 gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca tacagcatgc
    10621 gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct gttttgacgg
    10681 ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg cttactttaa
    10741 ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg ataagcgttg
    10801 ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa tttgattgat
    10861 attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg gcgccacttt
    10921 cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca cactcatttg
    10981 caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc gaaaactgat
    11041 ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca aatattgatt
    11101 ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt tcgcaatacc
    11161 attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag ggcttggcaa
    11221 gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct tgtcaaaggg
    11281 gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa aaagactagc
    11341 aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa ttgttgcaaa
    11401 tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg ccatttacta
    11461 ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac tgatgaatca
    11521 gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca aataccaata
    11581 gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa ttctattaaa
    11641 gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact acttaatttc
    11701 atactaactt caccaacact ttttccctca ctgatttctt tagaataaag aaagagcttt
    11761 tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact aattgcattt
    11821 aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact accttctagg
    11881 tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt ctcctttagc
    11941 ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc catagtaagt
    12001 agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta atattcctag
    12061 aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc gtttttttaa
    12121 aattgcttgt tatattaaat aatcttagta attttattat ataacatttt attatataac
    12181 aatttattat ataacaatga ttaacgtcat aaaaagtagt tttgaattca taatcgttta
    12241 aataaaatta taagatcagt tatttaaaca gctagtagac ataatgtgta tactaccaag
    12301 taacgcctgt accaagggat ccaggcgctg attttgtgaa tttttattgg tctaggttaa
    12361 aagctctt
//
