LOCUS       AP027232                4372 bp    DNA     circular BCT 14-MAR-2024
DEFINITION  Enterococcus faecium JARB-OU2377 plasmid pJAOU2377_5 DNA, complete
            sequence.
ACCESSION   AP027232
VERSION     AP027232.1
DBLINK      BioProject: PRJDB14951
            BioSample: SAMD00565591
            Sequence Read Archive: DRR427977, DRR428057
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Segawa,T., Masuda,K., Hisatsune,J., Kuroki,K. and Sugai,M.
  TITLE     Complete genome sequence of vanA-positive Enterococcus faecium
            strain JARB-OU2377
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Sugai,M. and Segawa,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Takaya Segawa National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center;
            4-2-1, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Flye v. 2.9.1
            Genome Coverage       :: 72x
            Sequencing Technology :: Illumina MiSeq; Nanopore GridION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JARB-OU2377"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJAOU2377_5"
                     /geo_loc_name="Japan:Hiroshima"
                     /collection_date="2020-07-09"
     gene            complement(746..2152)
                     /locus_tag="JARBOU2377_30530"
     CDS             complement(746..2152)
                     /locus_tag="JARBOU2377_30530"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42759.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSENEIEW"
                     /name="hypothetical protein"
     gene            complement(2374..2736)
                     /locus_tag="JARBOU2377_30540"
     CDS             complement(2374..2736)
                     /locus_tag="JARBOU2377_30540"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42760.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(2740..3048)
                     /locus_tag="JARBOU2377_30550"
     CDS             complement(2740..3048)
                     /locus_tag="JARBOU2377_30550"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42761.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(3057..3476)
                     /locus_tag="JARBOU2377_30560"
     CDS             complement(3057..3476)
                     /locus_tag="JARBOU2377_30560"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42762.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(3469..3702)
                     /locus_tag="JARBOU2377_30570"
     CDS             complement(3469..3702)
                     /locus_tag="JARBOU2377_30570"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42763.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(3705..>4372)
                     /locus_tag="JARBOU2377_30580"
     misc_feature    complement(3705..>4372)
                     /locus_tag="JARBOU2377_30580"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="partial CDS similar to WP_000277654.1:replication
                     initiation protein"
     Typing          complement(750..2152)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.094"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagaaatttc aatataggca atattttcgt catgttggtc taatagctgc caaccattat
       61 ttccaaccgc ttttaaatag ccattgcctt catttacccg cataagctgt tcaaaatcta
      121 atattaatac atcatgttga gtatgataaa aaatattttg attcaacttt ccaacaatgg
      181 taattcggtc aatcgaccaa tttaatttta tagtttgtac caaagtttga acatttaacc
      241 gcctgttaga cagggctgtt aatttttccc actcattcac gattgttctt tttttactca
      301 tattcagttc ctcctcaaag aaaaaagaga tttttggaaa gaatttccag agttcacgta
      361 actctaaaaa ttcataacca tcaatctcta gtcttgaaga aacctaaaaa tattcgtata
      421 atgaatttga gttacggaac tagtgtggtt agtttccgta aaggtcgaga acgttggtag
      481 cgttgctcga cttatttttt tgctcttttt ccagttcatt tgtttgctaa ctcaataata
      541 gcacttctct ttagctatgc aaataccaaa aaaataaaac cgcacccttt gaaaagggcg
      601 cccccaaact tcgctaacgc taagttggcc aaaaaatgga cttgccattt tttagcaaat
      661 gtcagcttca ggggctagtg gtttagccgt caactcctta tcctcggcac aaaccgcctc
      721 cggtaccgtt gactgcacat cgtggctacc actcaatctc attctcagaa cgctctaatt
      781 tatgctgtga gacgttttga cgataaaata aggttttctc ctgctctaat acttctaatt
      841 cctcagaaag ccccatatcc ttttctgcaa gaccaaaacg gatagacaaa cgagtgatcg
      901 atttttgaaa tgcgtttttt aatcgctcta aacgctggcg taaagagcga ttttcttctt
      961 tcagcttttt attttccact tgtaaatctg ctaattgttt tctaccagac aaatgacgct
     1021 cttcttccaa tgtagctaca tactcattta attctgtatt ttgctcaagc atctgtaaat
     1081 attgctcact catgttatca gcatttctct tggcatttgt tgctctcgta gctcgttgat
     1141 acaaatcaac aaaatcattc ccagataaat gatacgaacc atcaggaagc ctcttgaaac
     1201 tttctaaagg aaccatatct ttttggaaaa tgaccgccat ctcttttttt acattcagca
     1261 tatttttctt ctcttcttca atcttcgctc tctcagaagc catcagagcc ttatcacgct
     1321 cgttttcttc tctttgccta taaatatacc tcaactcatc agaaacgctc tcacgctctt
     1381 ttacagccgt ttggcgcaat tcagaaattt cactcatgat ttctttgtat tcgtgaatat
     1441 ctttaatttt gttggtaccg acaatatccc ttttccaacc gtaatcattc attaatcgtt
     1501 ctatagagtc aacttcttga tttctaaaat tattgaaaag cactcgacca ttatctttat
     1561 cagcgtgtat cccttgttga accagtgctt tattaaagct cggctgcttt tgaacacccc
     1621 gtttataacc ttcagcaacc ggcacaatat tcaagtgcag gtgcggactt atttcgtcat
     1681 tatgaataac agcattataa acagccaaat tagggtttct ctcttgaaac tccttgtaat
     1741 actgaaccaa aattttattg gcaatcactc gattttgttc agcttgttgt ttcgatatgc
     1801 cagtagaaga accatctgct cgatttgtct ggaaatcatc taaaatgcct atttgcacaa
     1861 taaattctcg ttgaggttcc aactttttat ccttgcgcac tttcgctaaa tagtcctcga
     1921 tttttctatc ggctcttttt tgtttcgcat tatatttttc taccacttcg ccaaacaatt
     1981 cctcatattt atctgctaga ttcgtctgct ctaaataaat attttcatcc gatttcgtga
     2041 agtcaatgtg cttatgccaa tcattggttt tctggcgatc agttaactca cgattattat
     2101 gagaaatact tgtattcttc gttccttttt taaatgaaat actaaacccc aagttaaaac
     2161 cctccttcgc gctgtcgcta ataaaaagat tgtaaacctt tctgttactc tttgtcaaag
     2221 agtaacattt tcctaattgt gacatggatg tcacaattcc aaacgctctc cgagggtttt
     2281 cgctgtcaca agtgacaacg aaaaaaggag ccgaaagcgt atcacgctat caactccttt
     2341 tatgctttat tttgcttcac gcaaaataat cgcttatcgt aaattcacgt cattaaatat
     2401 tgacgtaaat ttactctcac tcatctgact tttcttattc ggtatcactc cgaataagtc
     2461 gtcagattca tcgggataat ctcgttcgct tggcgctgat ttttcatcag caagccactc
     2521 attatccact gttttttcgt gttgctgatc gtcagaaatt gacgataacc accgctcaaa
     2581 atcagcaaaa gtttcaaacc caaaacgctt gcgaagagcc ttgcccattg cagcattttc
     2641 tttctcaatc aagcgttttt tctgttggcg aagtttcgct tgttcttttt ttaatttttc
     2701 ttcttgcgct ttaatatctg ctaaagtcat tcccataaac tacatctcct tttctttgga
     2761 attaataacg gaaatttggt ttaactgcat aacatgactc attaatttca accaaaattc
     2821 tttttctgat tcatcagttg tttgcgctaa atgcttatga acaaattcat tagtcaaaac
     2881 tgaagatttt gctagatcag cattgataac cgagttgaga acatccataa ttcgttcaag
     2941 catttcacta taattatctt catccggcaa attcaaataa tcagctaacc gttcatacat
     3001 caattcaatt ctatcgactg tttcttctct caaatcaatt ttatccatgt cacacctcac
     3061 ttccagaatt gccaccattt tcgatttttt aattcttgat tttctagtaa caactgatcg
     3121 tttttttgtt gctgaagcaa agtcaatttt tgttggtcac gaatcatttc cgttaattca
     3181 gaaatatgcc tatctttttc cgctaattgt tcacgcaaaa attcattttc tttttctaac
     3241 gggaaatcat tgcctgcatc attgaccggt aaatcaccag tcaattgacc accttttgac
     3301 cgataaattg accgcacaac accaatcaat tccttgtcta acatgataac cccattatct
     3361 ttataccgag caaggtcata acctgcattg accagttgat tgcgcactgt atttttagat
     3421 atacccaact catcagcaag gttttttatg gtcatttttt ctggcaaatc agtcattatc
     3481 ttctccgtta ctttcagcta ccccataaac atatttaaca gcatgcttag ggataatgat
     3541 tggggaatca attttattat ttttcaaaat caactcatac tgcgttacat caagtaactt
     3601 accggacatt tttttgctat ccaaaaaaac aacgacaacg ttatcacctg ttaaattcac
     3661 caaccaattt ttcttaccaa aatgcttttg tttttctgcc attactattc ctcctctgtc
     3721 acatcaagtc cctgtaacca agtatccaat tcttttttta aatcatctgc tgattcttca
     3781 aatgtttctc gtaaatgatt cgtcaactca tcattttgac tttctcgctt taataaattt
     3841 cgatatttat atttcgagtt tcttgacaaa atcgcccaat tagaatgatt agctaacaat
     3901 ccatcaatca taactttatc cgtaggtttt atatcagttg gtaaaaaatg tggactcaca
     3961 aagctatcca aactttcttt caccatagca taccattccg tagctttacc acgtcttaac
     4021 tgcatttcta accgccacca agtattaatt tctgaaggca ccacctttct tttcatttct
     4081 tgctctattt ttttattgta caaacgaatt tgccgctcgc ttgcccgact accccaatac
     4141 accgtttcca gttttcctga gcgtccataa attaatttaa atgaaactgg atcaataatt
     4201 cgatattgcg tgataaactc atcaggcaca tcaataagat cgcaggcaac atccgctcgt
     4261 gaaaaatggg gctctaaaaa taaatcatga ataaaatttt tcatggaatc cgctaaaaat
     4321 cgactgattt tattaggatt aaaatcaatc cgcccttttc cttcttgcca tt
//
