LOCUS       AP027231               12370 bp    DNA     circular BCT 14-MAR-2024
DEFINITION  Enterococcus faecium JARB-OU2377 plasmid pJAOU2377_4 DNA, complete
            sequence.
ACCESSION   AP027231
VERSION     AP027231.1
DBLINK      BioProject: PRJDB14951
            BioSample: SAMD00565591
            Sequence Read Archive: DRR427977, DRR428057
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Segawa,T., Masuda,K., Hisatsune,J., Kuroki,K. and Sugai,M.
  TITLE     Complete genome sequence of vanA-positive Enterococcus faecium
            strain JARB-OU2377
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   Sugai,M. and Segawa,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Takaya Segawa National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center;
            4-2-1, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Flye v. 2.9.1
            Genome Coverage       :: 72x
            Sequencing Technology :: Illumina MiSeq; Nanopore GridION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..12370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JARB-OU2377"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJAOU2377_4"
                     /geo_loc_name="Japan:Hiroshima"
                     /collection_date="2020-07-09"
     gene            1201..2127
                     /gene="rep_1"
                     /locus_tag="JARBOU2377_30360"
     CDS             1201..2127
                     /gene="rep_1"
                     /locus_tag="JARBOU2377_30360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDW42742.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKLLKNKQSPHFRKKRKKVYYLI"
                     /name="rep_1"
     gene            2165..2698
                     /locus_tag="JARBOU2377_30370"
     CDS             2165..2698
                     /locus_tag="JARBOU2377_30370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDW42743.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            2713..2883
                     /locus_tag="JARBOU2377_30380"
     CDS             2713..2883
                     /locus_tag="JARBOU2377_30380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42744.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            3353..3577
                     /locus_tag="JARBOU2377_30390"
     CDS             3353..3577
                     /locus_tag="JARBOU2377_30390"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42745.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     gene            3597..3884
                     /locus_tag="JARBOU2377_30400"
     CDS             3597..3884
                     /locus_tag="JARBOU2377_30400"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42746.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            4334..4714
                     /gene="mobC"
                     /locus_tag="JARBOU2377_30410"
     CDS             4334..4714
                     /gene="mobC"
                     /locus_tag="JARBOU2377_30410"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDW42747.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="mobC"
     gene            4696..5610
                     /gene="mobA"
                     /locus_tag="JARBOU2377_30420"
     CDS             4696..5610
                     /gene="mobA"
                     /locus_tag="JARBOU2377_30420"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="BDW42748.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="mobA"
     gene            5558..5848
                     /locus_tag="JARBOU2377_30430"
     CDS             5558..5848
                     /locus_tag="JARBOU2377_30430"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748752.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42749.1"
                     /translation="MTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTT
                     IDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            5855..6544
                     /locus_tag="JARBOU2377_30440"
     CDS             5855..6544
                     /locus_tag="JARBOU2377_30440"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42750.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            7388..8332
                     /gene="rep_2"
                     /locus_tag="JARBOU2377_30450"
     CDS             7388..8332
                     /gene="rep_2"
                     /locus_tag="JARBOU2377_30450"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDW42751.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="rep_2"
     gene            8353..8886
                     /locus_tag="JARBOU2377_30460"
     CDS             8353..8886
                     /locus_tag="JARBOU2377_30460"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDW42752.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            8901..9071
                     /locus_tag="JARBOU2377_30470"
     CDS             8901..9071
                     /locus_tag="JARBOU2377_30470"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42753.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            9541..9765
                     /locus_tag="JARBOU2377_30480"
     CDS             9541..9765
                     /locus_tag="JARBOU2377_30480"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42754.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     gene            9785..10072
                     /locus_tag="JARBOU2377_30490"
     CDS             9785..10072
                     /locus_tag="JARBOU2377_30490"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42755.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            10657..10896
                     /locus_tag="JARBOU2377_30500"
     CDS             10657..10896
                     /locus_tag="JARBOU2377_30500"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     10656,10586,10646; possible pseudo due to internal stop
                     codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42756.1"
                     /translation="MPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWC
                     NTHQHDVDPGDAQRLSENLKIMQKELQKIWQQLK"
                     /name="hypothetical protein"
     gene            10878..11417
                     /locus_tag="JARBOU2377_30510"
     CDS             10878..11417
                     /locus_tag="JARBOU2377_30510"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 11363"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42757.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     MNLFGKTKFGLRLIKQ"
                     /name="hypothetical protein"
     gene            11583..12029
                     /locus_tag="JARBOU2377_30520"
     CDS             11583..12029
                     /locus_tag="JARBOU2377_30520"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW42758.1"
                     /translation="MSWTDENEPTINNEPTSYEELELAFAKLQKKYEENNELLRLNNRK
                     LDRLEETMTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQVKK
                     FHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     BGC             1..12370
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1202..2144
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.894"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7389..8332
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4697..5607
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10879..11465
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.673"
                     /coverage="100.0"
                     /evalue="1.59e-103"
ORIGIN
        1 gaatcaagaa aaacgtttga cggataaaaa catggaaaaa ctcactcgtc tttatcaaaa
       61 cttgccagaa aaacagcaaa aagaggttca agacaactat gagcgaacca aaaaagacgt
      121 gcaatatttt aaagatacgc agacagaaga agcttgttat aatttattca gtgatacaat
      181 ccctttttca actagtgaac aggaacgaaa agaacggcaa caatcagtta cctacgaacg
      241 ttataaagca ctgcttcagc aagcaacgaa catacaaaat ccaacgaaaa aagaaacggc
      301 attaaattat ctaaaaagtg tcaaggaatg gttagatcag caacagcaaa attgaatttg
      361 atcggcaatc atgttaaact aaacatgcaa atgatcaaac attagataca cgaaagcccc
      421 agagaggtcg gattctctgg ggcttttttt caggttgatg tcgcctttat tgattttact
      481 tcttgtttcg gttattcaac caataactga ataaagagac acaaacttct ataagaaggg
      541 ggcaaatgaa caattcaaac attagataca cctccttcca gtcgcaagac tgtcggatag
      601 gcgacaagtt attatatctt aaaaaattaa acttgtgtgg tgtttttaag tatcgcttat
      661 tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ttttaagaac aagataaggg
      721 atttatacta ggactgtctt aaaatgtctt aaacgaacgc tcagagcttc tcaaagggtt
      781 ccttatgaac ggcttttagc aaaggagccg gaggcgactg cgcagtataa tgtgagagca
      841 gagcgatcac acctttttag cgaaccgtag ggagcaaggt ttggaattgg cagttttgat
      901 aaattcgcga acctgtttag agaaaaaagg agtgattttt taggcgggaa aattagcctc
      961 ccctcttttt atatctcttt ttatatctct ttttaaacct ctttaccgta agctgtgtcc
     1021 ttactctacc aatggataca gaatgttatg aaggacattt tgtctgttta tgaaggacat
     1081 tttgtctgtt tatgaaggac attttgtctg tttatgaagg acattttgtc tgtttgaacg
     1141 tcaaatatga atgtcttgac atttaaaaaa tgtcattcat aattggattg aggtgattat
     1201 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
     1261 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
     1321 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
     1381 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
     1441 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
     1501 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
     1561 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
     1621 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
     1681 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
     1741 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
     1801 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
     1861 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     1921 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
     1981 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
     2041 caactagaaa aagagaaaaa gctcttgaaa aacaaacaga gtccgcactt tcggaaaaag
     2101 agaaaaaaag tttattatct gatttagata atttattcca gtagcaacct aggaggcaac
     2161 cagtatgcaa ccacaagaaa cctttacaat aaaggaattg tcagatcttt ttaagatgag
     2221 taggcaagca atatcgaaac atatccaaaa attagattct tccatgatag ctaaaaatga
     2281 gcgtggttat aaggttgtat tgcgttctgg agtattgcaa cttgctcgca acttagacaa
     2341 tcaaaggttg ctagataagt tgcataaaca tgaacaaaac aacgataaaa atgcttatat
     2401 accaaacaaa aacgtggttg caaacaagtt gctagatcag ttgcgtgaag aaaatgaatt
     2461 tttacgagaa caacttgccc tcaaagagca aaataattct catatacaaa aactactcga
     2521 ccagcaacaa caactcaccc tgcaagccaa ccaacaaatc gaaaaacttc aggagcaact
     2581 gcagttgact tatacggaag agtcttcaga gaatagttca acgactcttt ctgaagcaac
     2641 agaaacaatg gacgaacaaa caaagaattg gaaaaaatgg tggcagctat ggaaatgaag
     2701 aaggaaatac ttatgacaaa aaaacacttg ctattcggtt ctatcctact aggaatatta
     2761 agctttagcc caatgttatt atcagtggcc aacaaaggaa ataaaaaagg ggaactaaaa
     2821 ctcaatccta attactacaa cagtcaaaat gaggaagtat ttgtagaaaa acttgataaa
     2881 taagaaaaga ctgctcgtac gagcagtctt tctgaaagag attatcctat tattaacctt
     2941 agaaaagaat aataaatgaa tatgagatat gtaaaaagca taacaaacac aaacaaaaat
     3001 aacaagtatt taaaaaatga ccgatcaata ttcacaaaga attagaaaga aacaaccgca
     3061 gaaaaccaag ccttaaaaag agcttttaac ctagaccaat aaaaattcac aaaatcagcg
     3121 cctggatccc ttggtacagg cgttacttgg tagtatacac attatgtcta ctagctgttt
     3181 aaataactga tcttataatt ttatttaaac gattatgaat tcaaaactac tttttatgac
     3241 gttaatcatt gttatataat aaattgttat ataataaaat gttatataat aaaattacta
     3301 agattattta atataacaag caattttaaa aaaacgaaaa ggaaaaacaa tcatgaaaaa
     3361 gaaagtatta aaacattgtg ttattctagg aatattagga acttgtctag ctggcatcgg
     3421 tacaggaata aaagttgatg cagctactta ctatggaaat ggtctttatt gtaacaaaga
     3481 aaaatgttgg gtagattgga atcaagctaa aggagaaatt ggaaaaatta ttgttaatgg
     3541 ttgggttaat catggtccat gggcacctag aaggtagtgg tagggaggtt attataatgg
     3601 attttactaa agaagaaaaa cttttaaatg caattagtaa agtatacaat gaagcaacta
     3661 tagatgacta tcctgactta aaagaaaagc tctttcttta ttctaaagaa atcagtgagg
     3721 gaaaaagtgt tggtgaagtt agtatgaaat taagtagttt tcttggaaga tatattttaa
     3781 aacataaatt tggattacct aaatctttaa tagaattaca agaaattgtt agtaaggaat
     3841 ctcaagtata tagaggatgg gcttctattg gtatttggag ttaattcttt ggaataaaat
     3901 gaaaatggga ctgaagaatc aattctgatt catcagtccc ataaattata gagccgaaaa
     3961 attatcatat ttatagattt attaatagta aatggcataa gaatctttga acaacgaaaa
     4021 aaatgtagct ctacctaatt attgatttgc aacaatttca ccgaaggtga aacaagcgaa
     4081 actttacaaa aagtggctag catttgctag tcttttttgt attgtggaga gtggggggga
     4141 gggggattca agggggagct tgcgcccctt tgacaagcgg ttatttgcac caaatacgta
     4201 ggtatttggc tagcaaatat agagcttgcc aagcccttta gtgagcttta gtgagcttta
     4261 cttattgtac ctttttaggt acaatggtat tgcgaacgat acgtccgtag gacgtactga
     4321 aagggtggga cttatgagcg aacaaaatca atatttggct agcaatcatt ctaaaaaata
     4381 tacctatcga tccgagccaa aacaaatcag ttttcgagtg agcgaatccg aatttgcaaa
     4441 gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg gctttcgtga aagccaaggc
     4501 acaaaacgcg cgtctggtga caccgaaagt ggcgccagac attgctcagg caatcgcgcg
     4561 cgatttagcc aaagctggcg ggaatatcaa tcaaattgcc aagtggtgca acacacacca
     4621 acacgacgtt gatcctggcg acgcccaacg cttatccgaa aacctaaaaa tcatgcaaaa
     4681 ggagctacaa aagatatggc aacagttaaa gtaagccgaa ccacttcttg tagccgagcg
     4741 atcaattacg ccgaaccacg agcaaccgtc aaaacaggga taaactgtga cattgattat
     4801 gccaaaagcg aaatgaaaca aattcgcatg ctgtatggaa aagacgatcg tgtacaagca
     4861 cacttattga tccagtcttt tcgaccagga gaaattaccg ctgaaaaagc caatcaacta
     4921 ggggcagagt atgcgaaaaa aatagcccct gaccaccaaa tcgcgattta cacacataca
     4981 gacaaagacc atattcataa ccacattgtc attaactccg tcaatctgga aactgggaac
     5041 aaatttcaag cccatggtca agcattttta aacaaatgtt atgacctaaa tgacgagatt
     5101 tgtcttgaac atgacttaac aatcactgaa cgagggaaga aacccgaaaa acgaaccatg
     5161 tctgaaatca aattaaaaga aaaaaatgaa cctgtttgga aagacgaaat tcggtttgcg
     5221 attgatcaaa caatgaaaaa caaaaaaact cgaacgtata accaattttg tgattctctt
     5281 aaaacattcg gcattcattg cttgaatcgt gggaaaaatt ttacttacga attgatccac
     5341 aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt acgaaaagga gacgatttta
     5401 catgagctgg acagacgaga acgagcctac aatcaacaac gaaccaacga gctacgaaga
     5461 gctcgagcta gcattcgcga aactgcaaaa gaagtacgaa gagaacaacg aactcttacg
     5521 actgaacaac cggaaattag accgactcga agaaaccatg accaacacgt tgaaagagga
     5581 gcagactacc aacaagaatt atcgttctaa aacgttacaa gacatagaaa atgaaataaa
     5641 gaaacaacgt gcttttcttg caccttatac caaagaaaga atacaaacta caattgattc
     5701 acaagtcaaa aaattccacc atgcgctatt aaaccagcga aataaagaac agaaaaaagt
     5761 aattatgcag cgatcaatgg atattcttgc ccccttatgt tgcttaatca tgacactgtt
     5821 agccttttgg ctggtatggg gagaataaat agaaatggca ttctatttac cctatctact
     5881 catttttgcc agtattttag gaattatttg gcttagctat aaggcttatc aaacaagata
     5941 ttcattaaaa ggctctaaaa ttcgatttaa gaggttttta ttgccagggg ttatatttac
     6001 attatcaatc gttttaagtg gtttattcgg cgttttagag ggaaataaac gtatttcaag
     6061 aagtatttta ttaggggata tcactcaaaa gtatgaagca gcatgtgata aaaagaaaaa
     6121 ggagcaagtg ttaacacaaa aaatggaaga gtttaccact tgttatgagg aaatgaacga
     6181 tatttttgtg aatcaagaaa aacgtttgac ggataaaaac atggaaaaac tcactcgtct
     6241 ttatcaaaac ttgccagaaa aacagcaaaa agaggttcaa gacaactatg agcgaaccaa
     6301 aaaagacgtg caatatttta aagatacgca gacagaagaa gcttgttata atttattcag
     6361 tgatacaatc cctttttcaa ctagtgaaca ggaacgaaaa gaacggcaac aatcagttac
     6421 ctacgaacgt tataaagcac tgcttcagca agcaacgaac atacaaaatc caacgaaaaa
     6481 agaaacggca ttaaattatc taaaaagtgt caaggaatgg ttagatcagc aacagcaaaa
     6541 ttgaatttga tcggcaatca tgttaaacta aacatgcaaa tgatcaaaca ttagatacac
     6601 gaaagcccca gagaggtcgg attctctggg gctttttttc aggttgatgt cgcctttatt
     6661 gattttactt cttgtttcgg ttattcaacc aataactgaa taaagagaca caaacttcta
     6721 taagaagggg gcaaatgaac aattcaaaca ttagatacac ctccttccag tcgcaagact
     6781 gtcggatagg cgacaagtta ttatatctta aaaaattaaa cttgtgtggt gtttttaagt
     6841 atcgcttatt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaaagcta
     6901 gataaggatt tttatccaag actgtcttaa aacggcttag ataagcattc agaaccgctc
     6961 aaaggcctct ttaccaacat gtttttagca aaggagcctt taggcgactg cgcagtacaa
     7021 tgtgagagcg tagcgatcac acctttttgg cgaccgtagg aagcaaggtt ttggattggc
     7081 agttttgaaa aatgcgcgaa cctgtttaaa gaaaaaagaa gtggttttta ggcgggaaaa
     7141 ttagcccccc tctttttata tctcttttta aacctctttt taaacctctt taccgtaagc
     7201 tgtgtcctta ctctaccaat ggatacagaa tgttatgaag gacattttgt ctgtttatga
     7261 aggacatttt gtctgtttat gaaggacatt ttgtctgttt atgaaggaca ttttgtctgt
     7321 ttgaacgtca aatatgaatg tcttgacatt taaaaaatgt cattcataat tggattgagg
     7381 tgattatatg gctaatgaga ttgtaaaata ccaccatgaa ttaaatacaa ttcctttaag
     7441 aaaatttaca ccggttgaaa tgaatttgtt tttttccatc gtgtctagaa tgcgtgaaaa
     7501 aggggataag acagttcgtt ttacgtttga ccaactaaaa gatttaagta actacaaagc
     7561 aactgcaaat gtacgatttg tagaagattt aaaagcaact tatgaaaaaa tcttatcttt
     7621 acgctttggt cgtagaagtg agtctgggct ttcttacgat atgtttgtaa tgtttacaga
     7681 atttaaaatc aacggggacg tagaaaatcc ttacgtagat atacgaattt acgaaaaagc
     7741 actgcctttg ttaaataatt tagacgagtg ggtacgctat tctttacaac aattcaacga
     7801 gctacaaagt ggctatgcaa agactatgtt tcgtctgtta aagcaataca gaaccaaagg
     7861 tttcgcatat ttctccaaag aagactttct tgaattatta gatattccta aaagttataa
     7921 gcaacctgat atagataaac gagttataaa acctatacgt caagaactaa ctgcaatctt
     7981 caaaggacta acaatcaaaa agaaatacgg aaaaggacgg ggaaaacctg ttattggcta
     8041 tcaatttacg ttcaaacctg agatcaaaaa agcagacgac tttaacaaac atgcaccaac
     8101 caaagaagac caaaataaag cattggcttt agctgataaa caaaaagaat acgaagaacc
     8161 cgctacaaca attaacgaaa aaatcaaaca gaagaaacga gaacttgaac tcctccaaca
     8221 aatcaaacaa ctagaaaaag agaaaaaagc tcttgaaaaa caaacagagt ccgcactttc
     8281 ggaaaaagag aaaaaaagtt tattatctga tttagataat ttattccagt agcaacctag
     8341 gaggcaacca gtatgcaacc acaagaaacc tttacaataa aggaattgtc agatcttttt
     8401 aagatgagta ggcaagcaat atcgaaacat atccaaaaat tagattcttc catgatagct
     8461 aaaaatgagc gtggttataa ggttgtattg cgttctggag tattgcaact tgctcgcaac
     8521 ttagacaatc aaaggttgct agataagttg cataaacatg aacaaaacaa cgataaaaat
     8581 gcttatatac caaacaaaaa cgtggttgca aacaagttgc tagatcagtt gcgtgaagaa
     8641 aatgaatttt tacgagaaca acttgccctc aaagagcaaa ataattctca tatacaaaaa
     8701 ctactcgacc agcaacaaca actcaccctg caagccaacc aacaaatcga aaaacttcag
     8761 gagcaactgc agttgactta tacggaagag tcttcagaga atagttcaac gactctttct
     8821 gaagcaacag aaacaatgga cgaacaaaca aagaattgga aaaaatggtg gcagctatgg
     8881 aaatgaagaa ggaaatactt atgacaaaaa aacacttgct attcggttct atcctactag
     8941 gaatattaag ctttagccca atgttattat cagtggccaa caaaggaaat aaaaaagggg
     9001 aactaaaact caatcctaat tactacaaca gtcaaaatga ggaagtattt gtagaaaaac
     9061 ttgataaata agaaaagact gctcgtacga gcagtctttc tgaaagagat tatcctatta
     9121 ttaaccttag aaaagaataa taaatgaata tgagatatgt aaaaagcata acaaacacaa
     9181 acaaaaataa caagtattta aaaaatgacc gatcaatatt cacaaagaat tagaaagaaa
     9241 caaccgcaga aaaccaagcc ttaaaaagag cttttaacct agaccaataa aaattcacaa
     9301 aatcagcgcc tggatccctt ggtacaggcg ttacttggta gtatacacat tatgtctact
     9361 agctgtttaa ataactgatc ttataatttt atttaaacga ttatgaattc aaaactactt
     9421 tttatgacgt taatcattgt tatataataa attgttatat aataaaatgt tatataataa
     9481 aattactaag attatttaat ataacaagca attttaaaaa aacgaaaagg aaaaacaatc
     9541 atgaaaaaga aagtattaaa acattgtgtt attctaggaa tattaggaac ttgtctagct
     9601 ggcatcggta caggaataaa agttgatgca gctacttact atggaaatgg tctttattgt
     9661 aacaaagaaa aatgttgggt agattggaat caagctaaag gagaaattgg aaaaattatt
     9721 gttaatggtt gggttaatca tggtccatgg gcacctagaa ggtagtggta gggaggttat
     9781 tataatggat tttactaaag aagaaaaact tttaaatgca attagtaaag tatacaatga
     9841 agcaactata gatgactatc ctgacttaaa agaaaagctc tttctttatt ctaaagaaat
     9901 cagtgaggga aaaagtgttg gtgaagttag tatgaaatta agtagttttc ttggaagata
     9961 tattttaaaa cataaatttg gattacctaa atctttaata gaattacaag aaattgttag
    10021 taaggaatct caagtatata gaggatgggc ttctattggt atttggagtt aattctttgg
    10081 aataaaatga aaatgggact gaagaatcaa ttctgattca tcagtcccat aaattataga
    10141 gccgaaaaat tatcatattt atagatttat taatagtaaa tggcataaga atctttgaac
    10201 aacgaaaaaa atgtagctct acctaattat tgatttgcaa caatttcacc ttcggtgaaa
    10261 taagcgaaac tttacaaaaa gtggctagca aatgctagcc tttttttgta ttgtggagcg
    10321 tggattttga gggggattca agggggagct tgcgctcctt tgacaagcgg ttatttgcac
    10381 caaatacgga ggtatttggc tagcaaatat agagcttgcc aaaccctttt agtgagtttt
    10441 gtagaaagta cctttaaagg tactttcaca ttacgaacga tacgtccgta ggacgtactg
    10501 aaagggtggg acttatgagc aaattaatgc aaaatttggc tagcaaaaaa gctaaaaaat
    10561 atatgctaat aatcgagcga aataaacact ttttcaagtg agcgaatccg ccatgacaaa
    10621 gctgaagcag tcagctgaag ctttgtcaaa tggcgtgtgc cggctttcgt gaaagccaag
    10681 gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag acattgctca ggcaatcgcg
    10741 cgcgatttag ccaaagctgg cgggaatatc aatcaaattg ccaagtggtg caacacacac
    10801 caacacgacg ttgatcctgg cgacgcccaa cgcttatccg aaaacctaaa aatcatgcaa
    10861 aaggagctac aaaagatatg gcaacagtta aagtaagccg aaccacttct tgtagccgag
    10921 cgatcaatta cgccgaacca cgagcaaccg tcaaaacagg gataaactgt gacattgatt
    10981 atgccaaaag cgaaatgaaa caaattcgca tgctgtatgg aaaagacgat cgtgtacaag
    11041 cacacttatt gatccagtct tttcgaccag gagaaattac cgctgaaaaa gccaatcaac
    11101 taggggcaga gtatgcgaaa aaaatagccc ctgaccacca aatcgcgatt tacacacata
    11161 cagacaaaga ccatattcat aaccacattg tcattaactc cgtcaatctg gaaactggga
    11221 acaaatttca agcccatggt caagcatttt taaacaaatg ttatgaccta aatgacgaga
    11281 tttgtcttga acatgactta acaatcactg aacgagggaa gaaacccgaa aaacgaacca
    11341 tgtctgaaat caaattaaaa gaaaaaatga acctgtttgg aaagacgaaa ttcggtttgc
    11401 gattgatcaa acaatgaaaa acaaaaaaac tcgaacgtat aaccaatttt gtgattctct
    11461 taaaacattc ggcattcatt gcttgaatcg tgggaaaaat tttacttacg aattgatcca
    11521 caaaaagaaa aaagtacgat ccaacaaact aggaaaggat tacgaaaagg agacgatttt
    11581 acatgagctg gacagacgag aacgagccta caatcaacaa cgaaccaacg agctacgaag
    11641 agctcgagct agcattcgcg aaactgcaaa agaagtacga agagaacaac gaactcttac
    11701 gactgaacaa ccggaaatta gaccgactcg aagaaaccat gaccaacacg ttgaaagagg
    11761 agcagactac caacaagaat tatcgttcta aaacgttaca agacatagaa aatgaaataa
    11821 agaaacaacg tgcttttctt gcaccttata ccaaagaaag aatacaaact acaattgatt
    11881 cacaagtcaa aaaattccac catgcgctat taaaccagcg aaataaagaa cagaaaaaag
    11941 taattatgca gcgatcaatg gatattcttg cccccttatg ttgcttaatc atgacactgt
    12001 tagccttttg gctggtatgg ggagaataaa tagaaatggc attctattta ccctatctac
    12061 tcatttttgc cagtatttta ggaattattt ggcttagcta taaggcttat caaacaagat
    12121 attcattaaa aggctctaaa attcgattta agaggttttt attgccaggg gttatattta
    12181 cattatcaat cgttttaagt ggtttattcg gcgttttaga gggaaataaa cgtatttcaa
    12241 gaagtatttt attaggggat atcactcaaa agtatgaagc agcatgtgat aaaaagaaaa
    12301 aggagcaagt gttaacacaa aaaatggaag agtttaccac ttgttatgag gaaatgaacg
    12361 atatttttgt
//
