LOCUS       AP027226                8744 bp    DNA     circular BCT 14-MAR-2024
DEFINITION  Enterococcus faecium JARB-OU2352 plasmid pJAOU2352_5 DNA, complete
            sequence.
ACCESSION   AP027226
VERSION     AP027226.1
DBLINK      BioProject: PRJDB14951
            BioSample: SAMD00565567
            Sequence Read Archive: DRR427953, DRR428056
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Segawa,T., Masuda,K., Hisatsune,J., Kuroki,K. and Sugai,M.
  TITLE     Complete genome sequence of vanA-positive Enterococcus faecium
            strain JARB-OU2352
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Sugai,M. and Segawa,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Takaya Segawa National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center;
            4-2-1, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Flye v. 2.9.1
            Genome Coverage       :: 75x
            Sequencing Technology :: Illumina MiSeq; Nanopore GridION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JARB-OU2352"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJAOU2352_5"
                     /geo_loc_name="Japan:Hiroshima"
                     /collection_date="2020-11-18"
     gene            complement(726..2132)
                     /locus_tag="JARBOU2352_30070"
     CDS             complement(726..2132)
                     /locus_tag="JARBOU2352_30070"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39696.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSENEIEW"
                     /name="hypothetical protein"
     gene            complement(2354..2716)
                     /locus_tag="JARBOU2352_30080"
     CDS             complement(2354..2716)
                     /locus_tag="JARBOU2352_30080"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39697.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(2720..3028)
                     /locus_tag="JARBOU2352_30090"
     CDS             complement(2720..3028)
                     /locus_tag="JARBOU2352_30090"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39698.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(3037..3456)
                     /locus_tag="JARBOU2352_30100"
     CDS             complement(3037..3456)
                     /locus_tag="JARBOU2352_30100"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39699.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(3449..3682)
                     /locus_tag="JARBOU2352_30110"
     CDS             complement(3449..3682)
                     /locus_tag="JARBOU2352_30110"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39700.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(3685..4653)
                     /locus_tag="JARBOU2352_30120"
     CDS             complement(3685..4653)
                     /locus_tag="JARBOU2352_30120"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDW39701.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVGK
                     LNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQE
                     GKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPV
                     SFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRG
                     KATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLL
                     KRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
                     /name="replication initiation protein"
     gene            complement(5097..6503)
                     /locus_tag="JARBOU2352_30130"
     CDS             complement(5097..6503)
                     /locus_tag="JARBOU2352_30130"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39702.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSENEIEW"
                     /name="hypothetical protein"
     gene            complement(6726..7088)
                     /locus_tag="JARBOU2352_30140"
     CDS             complement(6726..7088)
                     /locus_tag="JARBOU2352_30140"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39703.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSMFNDVSLR"
                     /name="hypothetical protein"
     gene            complement(7092..7400)
                     /locus_tag="JARBOU2352_30150"
     CDS             complement(7092..7400)
                     /locus_tag="JARBOU2352_30150"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39704.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(7409..7828)
                     /locus_tag="JARBOU2352_30160"
     CDS             complement(7409..7828)
                     /locus_tag="JARBOU2352_30160"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39705.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(7821..8054)
                     /locus_tag="JARBOU2352_30170"
     CDS             complement(7821..8054)
                     /locus_tag="JARBOU2352_30170"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39706.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(8057..>8744)
                     /locus_tag="JARBOU2352_30180"
     misc_feature    complement(8057..>8744)
                     /locus_tag="JARBOU2352_30180"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="partial CDS similar to WP_000277654.1:replication
                     initiation protein"
     Typing          complement(3693..4562)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.164"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(730..2132)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.094"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5101..6503)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.094"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atattttcgt catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag
       61 ccattgcctt catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga
      121 gtatgataaa aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa
      181 tttaatttta tagtttgtac caaagtttga acatttaacc gcctgttaga cagggctgtt
      241 aatttttccc actcattcac gattgttctt tttttactca tattcagttc ctcctcaaag
      301 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa ttcataacca
      361 tcaatctcta gtcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac
      421 tagtgtggtt agtttccgta aaggtcgaga acgttggtag cgttgctcga cttatttttt
      481 tgctcttttt ccagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc
      541 aaataccaaa aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc
      601 taagttggcc aaaaaatgga cttgccattt tttagcaaat gtcagcttca ggggctagtg
      661 gtttagccgt caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat
      721 cgtggctacc actcaatctc attctcagaa cgctctaatt tatgctgtga gacgttttga
      781 cgataaaata aggttttctc ctgctctaat acttctaatt cctcagaaag ccccatatcc
      841 ttttctgcaa gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt
      901 aatcgctcta aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact
      961 tgtaaatctg ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca
     1021 tactcattta attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca
     1081 gcatttctct tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc
     1141 ccagataaat gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aaccatatct
     1201 ttttggaaaa tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca
     1261 atcttcgctc tctcagaagc catcagagcc ttatcacgct cgttttcttc tctttgccta
     1321 taaatatacc tcaactcatc agaaacgctc tcacgctctt ttacagccgt ttggcgcaat
     1381 tcagaaattt cactcatgat ttctttgtat tcgtgaatat ctttaatttt gttggtaccg
     1441 acaatatccc ttttccaacc gtaatcattc attaatcgtt ctatagagtc aacttcttga
     1501 tttctaaaat tattgaaaag cactcgacca ttatctttat cagcgtgtat cccttgttga
     1561 accagtgctt tattaaagct cggctgcttt tgaacacccc gtttataacc ttcagcaacc
     1621 ggcacaatat tcaagtgcag gtgcggactt atttcgtcat tatgaataac agcattataa
     1681 acagccaaat tagggtttct ctcttgaaac tccttgtaat actgaaccaa aattttattg
     1741 gcaatcactc gattttgttc agcttgttgt ttcgatatgc cagtagaaga accatctgct
     1801 cgatttgtct ggaaatcatc taaaatgcct atttgcacaa taaattctcg ttgaggttcc
     1861 aactttttat ccttgcgcac tttcgctaaa tagtcctcga tttttctatc ggctcttttt
     1921 tgtttcgcat tatatttttc taccacttcg ccaaacaatt cctcatattt atctgctaga
     1981 ttcgtctgct ctaaataaat attttcatcc gatttcgtga agtcaatgtg cttatgccaa
     2041 tcattggttt tctggcgatc agttaactca cgattattat gagaaatact tgtattcttc
     2101 gttccttttt taaatgaaat actaaacccc aagttaaaac cctccttcgc gctgtcgcta
     2161 ataaaaagat tgtaaacctt tctgttactc tttgtcaaag agtaacattt tcctaattgt
     2221 gacatggatg tcacaattcc aaacgctctc cgagggtttt cgctgtcaca agtgacaacg
     2281 aaaaaaggag ccgaaagcgt atcacgctat caactccttt tatgctttat tttgcttcac
     2341 gcaaaataat cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac
     2401 tcatctgact tttcttattc ggtatcactc cgaataagtc gtcagattca tcgggataat
     2461 ctcgttcgct tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt
     2521 gttgctgatc gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gtttcaaacc
     2581 caaaacgctt gcgaagagcc ttgcccattg cagcattttc tttctcaatc aagcgttttt
     2641 tctgttggcg aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg
     2701 ctaaagtcat tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt
     2761 ttaactgcat aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg
     2821 tttgcgctaa atgcttatga acaaattcat tagtcaaaac tgaagatttt gctagatcag
     2881 cattgataac cgagttgaga acatccataa ttcgttcaag catttcacta taattatctt
     2941 catccggcaa attcaaataa tcagctaacc gttcatacat caattcaatt ctatcgactg
     3001 tttcttctct caaatcaatt ttatccatgt cacacctcac ttccagaatt gccaccattt
     3061 tcgatttttt aattcttgat tttctagtaa caactgatcg tttttttgtt gctgaagcaa
     3121 agtcaatttt tgttggtcac gaatcatttc cgttaattca gaaatatgcc tatctttttc
     3181 cgctaattgt tcacgcaaaa attcattttc tttttctaac gggaaatcat tgcctgcatc
     3241 attgaccggt aaatcaccag tcaattgacc accttttgac cgataaattg accgcacaac
     3301 accaatcaat tccttgtcta acatgataac cccattatct ttataccgag caaggtcata
     3361 acctgcattg accagttgat tgcgcactgt atttttagat atacccaact catcagcaag
     3421 gttttttatg gtcatttttt ctggcaaatc agtcattatc ttctccgtta ctttcagcta
     3481 ccccataaac atatttaaca gcatgcttag ggataatgat tggggaatca attttattat
     3541 ttttcaaaat caactcatac tgcgttacat caagtaactt accggacatt tttttgctat
     3601 ccaaaaaaac aacgacaacg ttatcacctg ttaaattcac caaccaattt ttcttaccaa
     3661 aatgcttttg tttttctgcc attactattc ctcctctgtc acatcaagtc cctgtaacca
     3721 agtatccaat tcttttttta aatcatctgc tgattcttca aatgtttctc gtaaatgatt
     3781 cgtcaactca tcattttgac tttctcgctt taataaattt cgatatttat atttcgagtt
     3841 tcttgacaaa atcgcccaat tagaatgatt agctaacaat ccatcaatca taactttatc
     3901 cgtaggtttt atatcagttg gtaaaaaatg tggactcaca aagctatcca aactttcttt
     3961 caccatagca taccattccg tagctttacc acgtcttaac tgcatttcta accgccacca
     4021 agtattaatt tctgaaggca ccacctttct tttcatttct tgctctattt ttttattgta
     4081 caaacgaatt tgccgctcgc ttgcccgact accccaatac accgtttcca gttttcctga
     4141 gcgtccataa attaatttaa atgaaactgg atcaataatt cgatattgcg tgataaactc
     4201 atcaggcaca tcaataagat cgcaggcaac atccgctcgt gaaaaatggg gctctaaaaa
     4261 taaatcatga ataaaatttt tcatggaatc cgctaaaaat cgactgattt tattaggatt
     4321 aaaatcaatc cgcccttttc cttcttgcca tttagaaatt tcaatatagg caatattttc
     4381 gtcatgttgg tctaatagct gccaaccatt atttccaacc gcttttaaat agccattgcc
     4441 ttcatttacc cgcataagct gttcaaaatc taatattaat acatcatgtt gagtatgata
     4501 aaaaatattt tgattcaact ttccaacaat ggtaattcgg tcaatcgacc aatttaattt
     4561 tatagtttgt accaaagttt gaacatttaa ccgcctgtta gacagggctg ttaatttttc
     4621 ccactcattc acgattgttc tttttttact catattcagt tcctcctcaa agaaaaaaga
     4681 gatttttgga aagaatttcc agagttcacg taactctaaa aattcataac catcaatctc
     4741 tagtcttgaa gaaacctaaa aatattcgta taatgaattt gagttacgga actagtgtgg
     4801 ttagtttccg taaaggtcga gaacgttggt agcgttgctc gacttatttt tttgctcttt
     4861 ttccagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatacca
     4921 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaagttgg
     4981 ccaaaaatgg acttgccatt ttttagcaaa tgtcagcttc aggggctagt ggtttagccg
     5041 tcaactcctt atcctcggca caaaccgcct ccggtaccgt tgactgcaca tcgtggctac
     5101 cactcaatct cattctcaga acgctctaat ttatgctgtg agacgttttg acgataaaat
     5161 aaggttttct cctgctctaa tacttctaat tcctcagaaa gccccatatc cttttctgca
     5221 agaccaaaac ggatagacaa acgagtgatc gatttttgaa atgcgttttt taatcgctct
     5281 aaacgctggc gtaaagagcg attttcttct ttcagctttt tattttccac ttgtaaatct
     5341 gctaattgtt ttctaccaga caaatgacgc tcttcttcca atgtagctac atactcattt
     5401 aattctgtat tttgctcaag catctgtaaa tattgctcac tcatgttatc agcatttctc
     5461 ttggcatttg ttgctctcgt agctcgttga tacaaatcaa caaaatcatt cccagataaa
     5521 tgatacgaac catcaggaag cctcttgaaa ctttctaaag gaaccatatc tttttggaaa
     5581 atgaccgcca tctctttttt tacattcagc atatttttct tctcttcttc aatcttcgct
     5641 ctctcagaag ccatcagagc cttatcacgc tcgttttctt ctctttgcct ataaatatac
     5701 ctcaactcat cagaaacgct ctcacgctct tttacagccg tttggcgcaa ttcagaaatt
     5761 tcactcatga tttctttgta ttcgtgaata tctttaattt tgttggtacc gacaatatcc
     5821 cttttccaac cgtaatcatt cattaatcgt tctatagagt caacttcttg atttctaaaa
     5881 ttattgaaaa gcactcgacc attatcttta tcagcgtgta tcccttgttg aaccagtgct
     5941 ttattaaagc tcggctgctt ttgaacaccc cgtttataac cttcagcaac cggcacaata
     6001 ttcaagtgca ggtgcggact tatttcgtca ttatgaataa cagcattata aacagccaaa
     6061 ttagggtttc tctcttgaaa ctccttgtaa tactgaacca aaattttatt ggcaatcact
     6121 cgattttgtt cagcttgttg tttcgatatg ccagtagaag aaccatctgc tcgatttgtc
     6181 tggaaatcat ctaaaatgcc tatttgcaca ataaattctc gttgaggttc caacttttta
     6241 tccttgcgca ctttcgctaa atagtcctcg atttttctat cggctctttt ttgtttcgca
     6301 ttatattttt ctaccacttc gccaaacaat tcctcatatt tatctgctag attcgtctgc
     6361 tctaaataaa tattttcatc cgatttcgtg aagtcaatgt gcttatgcca atcattggtt
     6421 ttctggcgat cagttaactc acgattatta tgagaaatac ttgtattctt cgttcctttt
     6481 ttaaatgaaa tactaaaccc caagttaaac ccctccttcg cgctgtcgct aataaaaaga
     6541 gtgtaaacct ttctgttact ctttgacaaa gagtaaacat tttcctaatt gtgacatggc
     6601 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     6661 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     6721 atcgcttatc gtaaactcac gtcattaaac attgacgtaa atttactctc actcatctga
     6781 cttttcttat tcggtatcac tccgaataag tcgtcagatt catcgggata atctcgttcg
     6841 cttggcgctg atttttcatc agcaagccac tcattatcca ctgttttttc gtgttgctga
     6901 tcgtcagaaa ttgacgataa ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     6961 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     7021 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     7081 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     7141 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     7201 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     7261 accgagttga gaacatccat aattcgttca agcatttcac tataattatc ttcatccggc
     7321 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     7381 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     7441 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     7501 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     7561 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     7621 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     7681 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     7741 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     7801 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     7861 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     7921 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atccaaaaaa
     7981 acaacgacaa cgttatcacc tgttaaattc accaaccaat ttttcttacc aaaatgcttt
     8041 tgtttttctg ccattactat tcctcctctg tcacatcaag tccctgtaac caagtatcca
     8101 attctttttt taaatcatct gctgattctt caaatgtttc tcgtaaatga ttcgtcaact
     8161 catcattttg actttctcgc tttaataaat ttcgatattt atatttcgag tttcttgaca
     8221 aaatcgccca attagaatga ttagctaaca atccatcaat cataacttta tccgtaggtt
     8281 ttatatcagt tggtaaaaaa tgtggactca caaagctatc caaactttct ttcaccatag
     8341 cataccattc cgtagcttta ccacgtctta actgcatttc taaccgccac caagtattaa
     8401 tttctgaagg caccaccttt cttttcattt cttgctctat ttttttattg tacaaacgaa
     8461 tttgccgctc gcttgcccga ctaccccaat acaccgtttc cagttttcct gagcgtccat
     8521 aaattaattt aaatgaaact ggatcaataa ttcgatattg cgtgataaac tcatcaggca
     8581 catcaataag atcgcaggca acatccgctc gtgaaaaatg gggctctaaa aataaatcat
     8641 gaataaaatt tttcatggaa tccgctaaaa atcgactgat tttattagga ttaaaatcaa
     8701 tccgcccttt tccttcttgc catttagaaa tttcaatata ggca
//
