LOCUS       AP027225                9370 bp    DNA     circular BCT 14-MAR-2024
DEFINITION  Enterococcus faecium JARB-OU2352 plasmid pJAOU2352_4 DNA, complete
            sequence.
ACCESSION   AP027225
VERSION     AP027225.1
DBLINK      BioProject: PRJDB14951
            BioSample: SAMD00565567
            Sequence Read Archive: DRR427953, DRR428056
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Segawa,T., Masuda,K., Hisatsune,J., Kuroki,K. and Sugai,M.
  TITLE     Complete genome sequence of vanA-positive Enterococcus faecium
            strain JARB-OU2352
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Sugai,M. and Segawa,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Takaya Segawa National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center;
            4-2-1, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Flye v. 2.9.1
            Genome Coverage       :: 75x
            Sequencing Technology :: Illumina MiSeq; Nanopore GridION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JARB-OU2352"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJAOU2352_4"
                     /geo_loc_name="Japan:Hiroshima"
                     /collection_date="2020-11-18"
     gene            414..611
                     /locus_tag="JARBOU2352_29980"
     CDS             414..611
                     /locus_tag="JARBOU2352_29980"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39687.1"
                     /translation="MISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQLYK
                     SGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            1761..2684
                     /locus_tag="JARBOU2352_29990"
     CDS             1761..2684
                     /locus_tag="JARBOU2352_29990"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39688.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="hypothetical protein"
     gene            2700..3239
                     /locus_tag="JARBOU2352_30000"
     CDS             2700..3239
                     /locus_tag="JARBOU2352_30000"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDW39689.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            3924..4097
                     /locus_tag="JARBOU2352_30010"
     CDS             3924..4097
                     /locus_tag="JARBOU2352_30010"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39690.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            4548..5081
                     /locus_tag="JARBOU2352_30020"
     CDS             4548..5081
                     /locus_tag="JARBOU2352_30020"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39691.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            5099..5296
                     /locus_tag="JARBOU2352_30030"
     CDS             5099..5296
                     /locus_tag="JARBOU2352_30030"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39692.1"
                     /translation="MISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQLYK
                     SGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            6446..7369
                     /locus_tag="JARBOU2352_30040"
     CDS             6446..7369
                     /locus_tag="JARBOU2352_30040"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39693.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="hypothetical protein"
     gene            7385..7924
                     /locus_tag="JARBOU2352_30050"
     CDS             7385..7924
                     /locus_tag="JARBOU2352_30050"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDW39694.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            8609..8782
                     /locus_tag="JARBOU2352_30060"
     CDS             8609..8782
                     /locus_tag="JARBOU2352_30060"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39695.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     Typing          1762..2684
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6447..7369
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagtacactg aagatggtat ggtcgtaact aaatatgagg atggttatag tgcggaagat
       61 tttgcaaatt cttcagatat catgacaaga gctgcagttg caagatgggg caaaaaggaa
      121 tatacaaata ttgctgtaac cactggagta gctgctaatg ctattaatgc tgccttttgg
      181 gcaggaggga aagctagcac ttctatgttt ggtatcccag gttgggcaat tggtgctttg
      241 ttaacaggtg caagttggac taaaaaaggt gcttctccag ggaatgcagt tgcaaaagaa
      301 tgggacaaaa atggtaatgg ctggattgga ttttattacc aaaggggata tgatgcagct
      361 ggaagagttg tagctactag atattcaacg ttataagtta aggagtgatg tttttgatat
      421 ctaaatcatt aaaatcatta aaaacattta ctaaaaagaa tccgcaaata gctactattc
      481 gtatttttat atttgctata gctgttgcta taattgttaa tatgctaagc ggagggcaat
      541 tatacaaatc tggtttattt ttagtgttaa tcttagttgt cttagacata ataaagtcta
      601 aaaaaaaata aatcgataca ttttagaaga atagggttgt ttgaatatta caaagcaaca
      661 atttcacctt cggtgaaata agcgaaactt tacaaaaagt ggctagcaaa tgctagcctt
      721 tttttgtatt gtggagcgtg gattttgagg gggattcaag ggggagcttg cgctcctttg
      781 acaagcggtt atttgcacca aatacggagg tatttggcta gcaaatatag agcttgccaa
      841 acccttttag tgagttttgt agaaagtacc tttaaaggta ctttcacatt acgaacgata
      901 cgtccgtagg acgtactgaa agggtgggac ttatgagcaa attaatgcaa aatttggcta
      961 gcaaaaaagc taaaaaatat atgctaataa tcgagcgaaa taaacacttt ttcaagtgag
     1021 cgaatccgcc atgacaaagc tgaagcagtt tatttcagct ttgtcatggc gtgtgccgac
     1081 ttttttaaaa gtctaggcac acgagcaaat atctttatca agctctaatt tgcgttttaa
     1141 gacgtttgaa acgcctaagt atataaatat ataagaatag gtttaagagt gcttaaatcg
     1201 aattttagag ggcatttaga caattttagg gttgttaggg cttgccctga ccgcctgtaa
     1261 ggcgttttaa gaacaagata agggatttat actaggactg tcttaaaatg tcttaaacga
     1321 acgctcagag cttctcaaag ggttccttat gaacggcttt tagcaaagga gccggaggcg
     1381 actgcgcagt ataatgtgag agcagagcga tcacaccttt ttagcgaacc gtagggagca
     1441 aggtttggaa ttggcagttt tgataaattc gcgaacctgt ttagagaaaa aaggagtgat
     1501 tttttaggcg ggaaaattag cctccctctt tttatatctc tttttatatc tctttttata
     1561 tctcttttat gtcggctgtg tccttactct cccaagcaat tcagagtttt ttcaagtagg
     1621 ttttctacgc ttttcaagta ggttttctac gcttttcaag taggttttct acgcttttca
     1681 agtaggtttt ccgacattat tgagtatttt tataaagtat gttactttta tttcatactt
     1741 tataaaagta ggagaagctt atggcaaatg aaattgtaaa atatgaaaat agattaaatc
     1801 atattcctct gagaaaattt aatagtcgtg aaatgaactt atttttttca attgcttcac
     1861 gtgtacgtga aaaaggtgta gatgagatta ttttttcatt tcacgatttg aaaaaactaa
     1921 gcggttatac agaacgtggg gaacaattta taaaagatct tagtagcacg tatgacaaat
     1981 tattaagtat taatgcttgg aatgatgatg gaaatacttt aactaaattt gttgctttca
     2041 caaaatatag cattatacga gataaacaac ttgttataat ttcagttaat ccagaattta
     2101 aaggactttt taatcaatta aagaactgga caagatttaa tctagagcaa tttactaatt
     2161 tggatagcac atattcaaaa actatgtttc gattattaaa acaatatcga actcaaggct
     2221 ggactgaatt ttccaaagaa aaattttttg atttactaga tattcctaaa agttattgga
     2281 atagtccgtc aaatattgac aaatttatct taaggccaat taaagaagaa ctcactccct
     2341 tatttaaagg attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa cctgttatcg
     2401 gttatcgttt tacttggaaa gctgaaatta accatgctga cgatttttcc aaagggaaac
     2461 aagaagattt acgtataaaa ttatttaata ttgatcacaa tggagaatta acacgagaag
     2521 aaaaatggcg cgctaaagat cgaatactaa atcttccctt aggcactcat gaagctgatt
     2581 ttaaacaaca acaacaaact gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac
     2641 tcaaacaaga tttactagga aatttacaaa acttattcga ttaagcttaa ggagtattta
     2701 tggcagataa aacaatcaaa gaacttgcgg aagaattagg agttagtaaa acagcaatta
     2761 acaaaaaagt ttctgacaaa aatcgaaaac tttggtttgc aaaaaacgga aacaagtttg
     2821 tgattaacga aaccggtcaa aaagctataa aatcaatgtt tttagtcgat aacgcaaact
     2881 taaatcgaaa actagttggc gaaaaatcaa aaaccaaaaa ccataaaaac accaaccaag
     2941 tttccgaatt aaaactcctt tctttcctag aaaaacagtt aaatcgaaaa gatatacagc
     3001 tacaagaaaa agataatcaa ataaaacaaa tgcaaaaatt actcgaccag caacaacaac
     3061 tcactctaca agcgaaccaa caaattgaga acctacaaga acagctccat ttaacttata
     3121 cagaagaaac tccaacaacc gaatcaactg ccttttctga aaaaaaagca gacaatacac
     3181 aaccacttat tgaaaaaaag tggtggcaaa tttggaaatc taatagaacc aatctctaaa
     3241 attttctaga agttagttcg gatagtagag gaaaaagttg tactacctga tggggagatc
     3301 actcaaattt ttatgagaaa gaaaaaatag tttacaactg taaacaatca atgtttatag
     3361 ttgtaaacta tcaaactata tggtgactta ttgcttaaaa taggttgaaa tctcactcaa
     3421 tttttggtgg aaaaacaaaa taaattctaa tcgattttgt tgaaaaagtc ataaaaaaac
     3481 aaatgtgatt ttaacaaaat ttcaaaaaaa tcgtttgaaa tttcgtttat tttttttgag
     3541 aattttgatc atcgtagggt cattgtagaa tcattatgag atcatcgtgg gatcatcgaa
     3601 taaagtatgg gaaaggcttg cggtgcttgg tattaagggc attctccgcc tcccaggttc
     3661 ttgaccattt tctctcaacg tggtaaaaat cccacttata agagaaaatg gtcaagaacc
     3721 taaacggtcg gcgaagaaaa cataccaagc accacaagca aaaaaagtta gagtaacttc
     3781 caaaaaagaa aaatttttta gaacaacttt tgaaaattta attttgattt atatccgttc
     3841 aatttaaaca tttgataaac tgaaggctag tatttaaaat gtgtatacaa gtggtagcgt
     3901 taaaaaaagt ggaggtataa cctatgttaa aaaaaactgt gttggtactg ggttctattt
     3961 ctctaggaat attaggtttt agccccatgc tattatccgt aattggaaaa aacaattcca
     4021 acggagaact taaattaaat tcaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     4081 aagagacgga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     4141 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     4201 atgaaaaata acaccccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     4261 gcaactgcag aaaatcaagc cttaaaaaga gcttttgact tagaccaatc gaaaatcaca
     4321 aagtgagccc ctgtatccct tggtagagag gctacttggc atagtacaca ttatgtcaac
     4381 aagaatttaa tataacatat tttcctaatg cattctaact ataccacatt atcaaaaata
     4441 taaattatct ttactaaata aaatattgac aagcctatac cttttgtttt atgattttca
     4501 caaagaaaac gttttctata tatttttctg tatcaggagg aaattctatg aaaaaattta
     4561 ctttaggggt agctttgtta actattgggg cagttgtttt accgtcaact actatagttt
     4621 atgcttcaga aaattcccaa gtggtatcat taaacgaaaa caatgctact gaaaatgtag
     4681 ttattgagta cactgaagat ggtatggtcg taactaaata tgaggatggt tatagtgcgg
     4741 aagattttgc aaattcttca gatatcatga caagagctgc agttgcaaga tggggcaaaa
     4801 aggaatatac aaatattgct gtaaccactg gagtagctgc taatgctatt aatgctgcct
     4861 tttgggcagg agggaaagct agcacttcta tgtttggtat cccaggttgg gcaattggtg
     4921 ctttgttaac aggtgcaagt tggactaaaa aaggtgcttc tccagggaat gcagttgcaa
     4981 aagaatggga caaaaatggt aatggctgga ttggatttta ttaccaaagg ggatatgatg
     5041 cagctggaag agttgtagct actagatatt caacgttata agttaaggag tgatgttttt
     5101 gatatctaaa tcattaaaat cattaaaaac atttactaaa aagaatccgc aaatagctac
     5161 tattcgtatt tttatatttg ctatagctgt tgctataatt gttaatatgc taagcggagg
     5221 gcaattatac aaatctggtt tatttttagt gttaatctta gttgtcttag acataataaa
     5281 gtctaaaaaa aaataaatcg atacatttta gaagaatagg gttgtttgaa tattacaaag
     5341 caacaatttc accttcggtg aaataagcga aactttacaa aaagtggcta gcaaatgcta
     5401 gccttttttt gtattgtgga gcgtggattt tgagggggat tcaaggggga gcttgcgctc
     5461 ctttgacaag cggttatttg caccaaatac ggaggtattt ggctagcaaa tatagagctt
     5521 gccaaaccct tttagtgagt tttgtagaaa gtacctttaa aggtactttc acattacgaa
     5581 cgatacgtcc gtaggacgta ctgaaagggt gggacttatg agcaaattaa tgcaaaattt
     5641 ggctagcaaa aaagctaaaa aatatatgct aataatcgag cgaaataaac actttttcaa
     5701 gtgagcgaat ccgccatgac aaagctgaag cagtttattt cagctttgtc atggcgtgtg
     5761 ccgacttttt aaaagtctag gcacacgagc aaatatcttt atcaagctct aatttgcgtt
     5821 ttaagacgtt tgaaacgcct aagtatataa atatataaga ataggtttaa gagtgcttaa
     5881 atcgaatttt agagggcatt tagacaattt tagggttgtt agggcttgcc ctgaccgcct
     5941 gtaaggcgtt ttaagaacaa gataagggat ttatactagg actgtcttaa aatgtcttaa
     6001 acgaacgctc agagcttctc aaagggttcc ttatgaacgg cttttagcaa aggagccgga
     6061 ggcgactgcg cagtataatg tgagagcaga gcgatcacac ctttttagcg aaccgtaggg
     6121 agcaaggttt ggaattggca gttttgataa attcgcgaac ctgtttagag aaaaaaggag
     6181 tgatttttta ggcgggaaaa ttagcctccc ctctttttat atctcttttt atatctcttt
     6241 ttatatctct tttatgtcgg ctgtgtcctt actctcccaa gcaattcaga gttttttcaa
     6301 gtaggttttc tacgcttttc aagtaggttt tctacgcttt tcaagtaggt tttctacgct
     6361 tttcaagtag gttttccgac attattgagt atttttataa agtatgttac ttttatttca
     6421 tactttataa aagtaggaga agcttatggc aaatgaaatt gtaaaatatg aaaatagatt
     6481 aaatcatatt cctctgagaa aatttaatag tcgtgaaatg aacttatttt tttcaattgc
     6541 ttcacgtgta cgtgaaaaag gtgtagatga gattattttt tcatttcacg atttgaaaaa
     6601 actaagcggt tatacagaac gtggggaaca atttataaaa gatcttagta gcacgtatga
     6661 caaattatta agtattaatg cttggaatga tgatggaaat actttaacta aatttgttgc
     6721 tttcacaaaa tatagcatta tacgagataa acaacttgtt ataatttcag ttaatccaga
     6781 atttaaagga ctttttaatc aattaaagaa ctggacaaga tttaatctag agcaatttac
     6841 taatttggat agcacatatt caaaaactat gtttcgatta ttaaaacaat atcgaactca
     6901 aggctggact gaattttcca aagaaaaatt ttttgattta ctagatattc ctaaaagtta
     6961 ttggaatagt ccgtcaaata ttgacaaatt tatcttaagg ccaattaaag aagaactcac
     7021 tcccttattt aaaggattaa ctattcgtaa aaaatatggt aaaggtcgag gtaaacctgt
     7081 tatcggttat cgttttactt ggaaagctga aattaaccat gctgacgatt tttccaaagg
     7141 gaaacaagaa gatttacgta taaaattatt taatattgat cacaatggag aattaacacg
     7201 agaagaaaaa tggcgcgcta aagatcgaat actaaatctt cccttaggca ctcatgaagc
     7261 tgattttaaa caacaacaac aaactgaaga aaaacaggca gaaaaacgag aactttcaaa
     7321 caaactcaaa caagatttac taggaaattt acaaaactta ttcgattaag cttaaggagt
     7381 atttatggca gataaaacaa tcaaagaact tgcggaagaa ttaggagtta gtaaaacagc
     7441 aattaacaaa aaagtttctg acaaaaatcg aaaactttgg tttgcaaaaa acggaaacaa
     7501 gtttgtgatt aacgaaaccg gtcaaaaagc tataaaatca atgtttttag tcgataacgc
     7561 aaacttaaat cgaaaactag ttggcgaaaa atcaaaaacc aaaaaccata aaaacaccaa
     7621 ccaagtttcc gaattaaaac tcctttcttt cctagaaaaa cagttaaatc gaaaagatat
     7681 acagctacaa gaaaaagata atcaaataaa acaaatgcaa aaattactcg accagcaaca
     7741 acaactcact ctacaagcga accaacaaat tgagaaccta caagaacagc tccatttaac
     7801 ttatacagaa gaaactccaa caaccgaatc aactgccttt tctgaaaaaa aagcagacaa
     7861 tacacaacca cttattgaaa aaaagtggtg gcaaatttgg aaatctaata gaaccaatct
     7921 ctaaaatttt ctagaagtta gttcggatag tagaggaaaa agttgtacta cctgatgggg
     7981 agatcactca aatttttatg agaaagaaaa aatagtttac aactgtaaac aatcaatgtt
     8041 tatagttgta aactatcaaa ctatatggtg acttattgct taaaataggt tgaaatctca
     8101 ctcaattttt ggtggaaaaa caaaataaat tctaatcgat tttgttgaaa aagtcataaa
     8161 aaaacaaatg tgattttaac aaaatttcaa aaaaatcgtt tgaaatttcg tttatttttt
     8221 ttgagaattt tgatcatcgt agggtcattg tagaatcatt atgagatcat cgtgggatca
     8281 tcgaataaag tatgggaaag gcttgcggtg cttggtatta agggcattct ccgcctccca
     8341 ggttcttgac cattttctct caacgtggta aaaatcccac ttataagaga aaatggtcaa
     8401 gaacctaaac ggtcggcgaa gaaaacatac caagcaccac aagcaaaaaa agttagagta
     8461 acttccaaaa aagaaaaatt ttttagaaca acttttgaaa atttaatttt gatttatatc
     8521 cgttcaattt aaacatttga taaactgaag gctagtattt aaaatgtgta tacaagtggt
     8581 agcgttaaaa aaagtggagg tataacctat gttaaaaaaa actgtgttgg tactgggttc
     8641 tatttctcta ggaatattag gttttagccc catgctatta tccgtaattg gaaaaaacaa
     8701 ttccaacgga gaacttaaat taaattcaaa ttattacaac agtcctaacg aagaattgtt
     8761 tgtaaaagag acggataaat aataaaaaag actgctcttt cgagcagcca gttaaaaaat
     8821 aatgagtgtt tattcttgaa aaagcaacgc gcttatgaag cgctaccaaa aatttatcat
     8881 attttatgaa aaataacacc ccagtaaaaa atgactggta aaaattcaca aagaaacgga
     8941 aagaagcaac tgcagaaaat caagccttaa aaagagcttt tgacttagac caatcgaaaa
     9001 tcacaaagtg agcccctgta tcccttggta gagaggctac ttggcatagt acacattatg
     9061 tcaacaagaa tttaatataa catattttcc taatgcattc taactatacc acattatcaa
     9121 aaatataaat tatctttact aaataaaata ttgacaagcc tatacctttt gttttatgat
     9181 tttcacaaag aaaacgtttt ctatatattt ttctgtatca ggaggaaatt ctatgaaaaa
     9241 atttacttta ggggtagctt tgttaactat tggggcagtt gttttaccgt caactactat
     9301 agtttatgct tcagaaaatt cccaagtggt atcattaaac gaaaacaatg ctactgaaaa
     9361 tgtagttatt
//
