LOCUS       AP027224               12309 bp    DNA     circular BCT 14-MAR-2024
DEFINITION  Enterococcus faecium JARB-OU2352 plasmid pJAOU2352_3 DNA, complete
            sequence.
ACCESSION   AP027224
VERSION     AP027224.1
DBLINK      BioProject: PRJDB14951
            BioSample: SAMD00565567
            Sequence Read Archive: DRR427953, DRR428056
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Segawa,T., Masuda,K., Hisatsune,J., Kuroki,K. and Sugai,M.
  TITLE     Complete genome sequence of vanA-positive Enterococcus faecium
            strain JARB-OU2352
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12309)
  AUTHORS   Sugai,M. and Segawa,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Takaya Segawa National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center;
            4-2-1, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Flye v. 2.9.1
            Genome Coverage       :: 75x
            Sequencing Technology :: Illumina MiSeq; Nanopore GridION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JARB-OU2352"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJAOU2352_3"
                     /geo_loc_name="Japan:Hiroshima"
                     /collection_date="2020-11-18"
     gene            288..536
                     /locus_tag="JARBOU2352_29820"
     CDS             288..536
                     /locus_tag="JARBOU2352_29820"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     728,533,773"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39671.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKK"
                     /name="hypothetical protein"
     gene            991..1437
                     /locus_tag="JARBOU2352_29830"
     CDS             991..1437
                     /locus_tag="JARBOU2352_29830"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39672.1"
                     /translation="MSWTDENEPTINNEPTSYEELELAFAKLQKKYEENNELLRLNNRK
                     LDRLEETMTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQVKK
                     FHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            1444..2133
                     /locus_tag="JARBOU2352_29840"
     CDS             1444..2133
                     /locus_tag="JARBOU2352_29840"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39673.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            2976..3920
                     /gene="rep_1"
                     /locus_tag="JARBOU2352_29850"
     CDS             2976..3920
                     /gene="rep_1"
                     /locus_tag="JARBOU2352_29850"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDW39674.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="rep_1"
     gene            3941..4474
                     /locus_tag="JARBOU2352_29860"
     CDS             3941..4474
                     /locus_tag="JARBOU2352_29860"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDW39675.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            4489..4659
                     /locus_tag="JARBOU2352_29870"
     CDS             4489..4659
                     /locus_tag="JARBOU2352_29870"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39676.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            5129..5353
                     /locus_tag="JARBOU2352_29880"
     CDS             5129..5353
                     /locus_tag="JARBOU2352_29880"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39677.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     gene            5372..5659
                     /locus_tag="JARBOU2352_29890"
     CDS             5372..5659
                     /locus_tag="JARBOU2352_29890"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39678.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            6100..6489
                     /gene="mobC"
                     /locus_tag="JARBOU2352_29900"
     CDS             6100..6489
                     /gene="mobC"
                     /locus_tag="JARBOU2352_29900"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDW39679.1"
                     /translation="MGLMSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC"
     gene            6471..7385
                     /gene="mobA"
                     /locus_tag="JARBOU2352_29910"
     CDS             6471..7385
                     /gene="mobA"
                     /locus_tag="JARBOU2352_29910"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="BDW39680.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="mobA"
     gene            7333..7623
                     /locus_tag="JARBOU2352_29920"
     CDS             7333..7623
                     /locus_tag="JARBOU2352_29920"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748752.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39681.1"
                     /translation="MTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTT
                     IDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            7630..8319
                     /locus_tag="JARBOU2352_29930"
     CDS             7630..8319
                     /locus_tag="JARBOU2352_29930"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39682.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            9163..10107
                     /gene="rep_2"
                     /locus_tag="JARBOU2352_29940"
     CDS             9163..10107
                     /gene="rep_2"
                     /locus_tag="JARBOU2352_29940"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDW39683.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="rep_2"
     gene            10178..10660
                     /locus_tag="JARBOU2352_29950"
     CDS             10178..10660
                     /locus_tag="JARBOU2352_29950"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDW39684.1"
                     /translation="MSRQAISKHIQKLDSSMIAKNERGYKVVLRSGVLQLARNLDNQRL
                     LDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQLALKEQNNSHIQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMDEQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            11314..11538
                     /locus_tag="JARBOU2352_29960"
     CDS             11314..11538
                     /locus_tag="JARBOU2352_29960"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39685.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     gene            11558..11845
                     /locus_tag="JARBOU2352_29970"
     CDS             11558..11845
                     /locus_tag="JARBOU2352_29970"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDW39686.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     BGC             129..12309
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2977..3920
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9164..10107
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6472..7382
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agttttcgag tgagcgaatc cgaatttgca aagctgaagc agtcagctga agctttgcaa
       61 atgagtgtgc cggctttcgt gaaagccaag gcacaaaacg cgcgtctggt gacaccgaaa
      121 gtggcgccag acattgctca ggcaatcgcg cgcgatttag ccaaagctgg cgggaatatc
      181 aatcaaattg ccaagtggtg caacacacac caacacgacg ttgatcctgg cgacgcccaa
      241 cgcttatccg aaaacctaaa aatcatgcaa aaggagctac aaaagatatg gcaacagtta
      301 aagtaagccg aaccacttct tgtagccgag cgatcaatta cgccgaacca cgagcaaccg
      361 tcaaaacagg gataaactgt gacattgatt atgccaaaag cgaaatgaaa caaattcgca
      421 tgctgtatgg aaaagacgat cgtgtacaag cacacttatt gatccagtct tttcgaccag
      481 gagaaattac cgctgaaaaa gccaatcaac taggggcaga gtatgcgaaa aaatagcccc
      541 tgaccaccaa atcgcgattt acacacatac agacaaagac catattcata accacattgt
      601 cattaactcc gtcaatctgg aaactgggaa caaatttcaa gcccatggtc aagcattttt
      661 aaacaaatgt tatgacctaa atgacgagat ttgtcttgaa catgacttaa caatcactga
      721 acgaggaaga aacccgaaaa acgaaccatg tctgaaatca aattaaaaga aaaaatgaac
      781 ctgtttggaa agacgaaatt cggtttgcga ttgatcaaac aatgaaaaac aaaaaaactc
      841 gaacgtataa ccaattttgt gattctctta aaacattcgg cattcattgc ttgaatcgtg
      901 ggaaaaattt tacttacgaa ttgatccaca aaaagaaaaa agtacgatcc aacaaactag
      961 gaaaggatta cgaaaaggag acgattttac atgagctgga cagacgagaa cgagcctaca
     1021 atcaacaacg aaccaacgag ctacgaagag ctcgagctag cattcgcgaa actgcaaaag
     1081 aagtacgaag agaacaacga actcttacga ctgaacaacc ggaaattaga ccgactcgaa
     1141 gaaaccatga ccaacacgtt gaaagaggag cagactacca acaagaatta tcgttctaaa
     1201 acgttacaag acatagaaaa tgaaataaag aaacaacgtg cttttcttgc accttatacc
     1261 aaagaaagaa tacaaactac aattgattca caagtcaaaa aattccacca tgcgctatta
     1321 aaccagcgaa ataaagaaca gaaaaaagta attatgcagc gatcaatgga tattcttgcc
     1381 cccttatgtt gcttaatcat gacactgtta gccttttggc tggtatgggg agaataaata
     1441 gaaatggcat tctatttacc ctatctactc atttttgcca gtattttagg aattatttgg
     1501 cttagctata aggcttatca aacaagatat tcattaaaag gctctaaaat tcgatttaag
     1561 aggtttttat tgccaggggt tatatttaca ttatcaatcg ttttaagtgg tttattcggc
     1621 gttttagagg gaaataaacg tatttcaaga agtattttat taggggatat cactcaaaag
     1681 tatgaagcag catgtgataa aaagaaaaag gagcaagtgt taacacaaaa aatggaagag
     1741 tttaccactt gttatgagga aatgaacgat atttttgtga atcaagaaaa acgtttgacg
     1801 gataaaaaca tggaaaaact cactcgtctt tatcaaaact tgccagaaaa acagcaaaaa
     1861 gaggttcaag acaactatga gcgaaccaaa aaagacgtgc aatattttaa agatacgcag
     1921 acagaagaag cttgttataa tttattcagt gatacaatcc ctttttcaac tagtgaacag
     1981 gaacgaaaag aacggcaaca atcagttacc tacgaacgtt ataaagcact gcttcagcaa
     2041 gcaacgaaca tacaaaatcc aacgaaaaaa gaaacggcat taaattatct aaaaagtgtc
     2101 aaggaatggt tagatcagca acagcaaaat tgaatttgat cggcaatcat gttaaactaa
     2161 acatgcaaat gatcaaacat tagatacacg aaagccccag agaggtcgga ttctctgggg
     2221 ctttttttca ggttgatgtc gcctttattg attttacttc ttgtttcggt tattcaacca
     2281 ataactgaat aaagagacac aaacttctat aagaaggggg caaatgaaca attcaaacat
     2341 tagatacacc tccttccagt cgcaagactg tcggataggc gacaagttat tatatcttaa
     2401 aaaattaaac ttgtgtggtg tttttaagta tcgcttattt tagggttgtt agggcttgcc
     2461 ctgaccgcct gtaaggcgct ttaaagctag ataaggattt ttatccaaga ctgtcttaaa
     2521 acggcttaga taagcattca gaaccgctca aaggcctctt taccaacatg tttttagcaa
     2581 aggagccttt aggcgactgc gcagtacaat gtgagagcgt agcgatcaca cctttttggc
     2641 gaccgtagga agcaaggttt tggattggca gttttgaaaa atgcgcgaac ctgtttaaag
     2701 aaaaaagaag tggtttttag gcgggaaaat tagccccctc tttttatatc tctttttaaa
     2761 cctcttttta aacctcttta ccgtaagctg tgtccttact ctaccaatgg atacagaatg
     2821 ttatgaagga cattttgtct gtttatgaag gacattttgt ctgtttatga aggacatttt
     2881 gtctgtttat gaaggacatt ttgtctgttt gaacgtcaaa tatgaatgtc ttgacattta
     2941 aaaaatgtca ttcataattg gattgaggtg attatatggc taatgagatt gtaaaatacc
     3001 accatgaatt aaatacaatt cctttaagaa aatttacacc ggttgaaatg aatttgtttt
     3061 tttccatcgt gtctagaatg cgtgaaaaag gggataagac agttcgtttt acgtttgacc
     3121 aactaaaaga tttaagtaac tacaaagcaa ctgcaaatgt acgatttgta gaagatttaa
     3181 aagcaactta tgaaaaaatc ttatctttac gctttggtcg tagaagtgag tctgggcttt
     3241 cttacgatat gtttgtaatg tttacagaat ttaaaatcaa cggggacgta gaaaatcctt
     3301 acgtagatat acgaatttac gaaaaagcac tgcctttgtt aaataattta gacgagtggg
     3361 tacgctattc tttacaacaa ttcaacgagc tacaaagtgg ctatgcaaag actatgtttc
     3421 gtctgttaaa gcaatacaga accaaaggtt tcgcatattt ctccaaagaa gactttcttg
     3481 aattattaga tattcctaaa agttataagc aacctgatat agataaacga gttataaaac
     3541 ctatacgtca agaactaact gcaatcttca aaggactaac aatcaaaaag aaatacggaa
     3601 aaggacgggg aaaacctgtt attggctatc aatttacgtt caaacctgag atcaaaaaag
     3661 cagacgactt taacaaacat gcaccaacca aagaagacca aaataaagca ttggctttag
     3721 ctgataaaca aaaagaatac gaagaacccg ctacaacaat taacgaaaaa atcaaacaga
     3781 agaaacgaga acttgaactc ctccaacaaa tcaaacaact agaaaaagag aaaaaagctc
     3841 ttgaaaaaca aacagagtcc gcactttcgg aaaaagagaa aaaaagttta ttatctgatt
     3901 tagataattt attccagtag caacctagga ggcaaccagt atgcaaccac aagaaacctt
     3961 tacaataaag gaattgtcag atctttttaa gatgagtagg caagcaatat cgaaacatat
     4021 ccaaaaatta gattcttcca tgatagctaa aaatgagcgt ggttataagg ttgtattgcg
     4081 ttctggagta ttgcaacttg ctcgcaactt agacaatcaa aggttgctag ataagttgca
     4141 taaacatgaa caaaacaacg ataaaaatgc ttatatacca aacaaaaacg tggttgcaaa
     4201 caagttgcta gatcagttgc gtgaagaaaa tgaattttta cgagaacaac ttgccctcaa
     4261 agagcaaaat aattctcata tacaaaaact actcgaccag caacaacaac tcaccctgca
     4321 agccaaccaa caaatcgaaa aacttcagga gcaactgcag ttgacttata cggaagagtc
     4381 ttcagagaat agttcaacga ctctttctga agcaacagaa acaatggacg aacaaacaaa
     4441 gaattggaaa aaatggtggc agctatggaa atgaagaagg aaatacttat gacaaaaaaa
     4501 cacttgctat tcggttctat cctactagga atattaagct ttagcccaat gttattatca
     4561 gtggccaaca aaggaaataa aaaaggggaa ctaaaactca atcctaatta ctacaacagt
     4621 caaaatgagg aagtatttgt agaaaaactt gataaataag aaaagactgc tcgtacgagc
     4681 agtctttctg aaagagatta tcctattatt aaccttagaa aagaataata aatgaatatg
     4741 agatatgtaa aaagcataac aaacacaaac aaaaataaca agtatttaaa aaatgaccga
     4801 tcaatattca caaagaatta gaaagaaaca accgcagaaa accaagcctt aaaaagagct
     4861 tttaacctag accaataaaa attcacaaaa tcagcgcctg gatcccttgg tacaggcgtt
     4921 acttggtagt atacacatta tgtctactag ctgtttaaat aactgatctt ataattttat
     4981 ttaaacgatt atgaattcaa aactactttt tatgacgtta atcattgtta tataataaat
     5041 tgttatataa taaaatgtta tataataaaa ttactaagat tatttaatat aacaagcaat
     5101 tttaaaaaaa cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac attgtgttat
     5161 tctaggaata ttaggaactt gtctagctgg catcggtaca ggaataaaag ttgatgcagc
     5221 tacttactat ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag attggaatca
     5281 agctaaagga gaaattggaa aaattattgt taatggttgg gttaatcatg gtccatgggc
     5341 acctagaagg tagtggtagg aggttattat aatggatttt actaaagaag aaaaactttt
     5401 aaatgcaatt agtaaagtat acaatgaagc aactatagat gactatcctg acttaaaaga
     5461 aaagctcttt ctttattcta aagaaatcag tgagggaaaa agtgttggtg aagttagtat
     5521 gaaattaagt agttttcttg gaagatatat tttaaaacat aaatttggat tacctaaatc
     5581 tttaatagaa ttacaagaaa ttgttagtaa ggaatctcaa gtatatagag gatgggcttc
     5641 tattggtatt tggagttaat tctttggaat aaaatgaaaa tgggactgaa gaatcaattc
     5701 tgattcatca gtcccataaa ttatagagcc gaaaaattat catatttata gatttattaa
     5761 tagtaaatgg cataagaatc tttgaacaac gaaaaaaatg tagctctacc taattattga
     5821 tttgcaacaa tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt
     5881 gctagtcttt tttgtattgt ggagagtggg ggggaggggg attcaagggg gagcttgcgc
     5941 ccctttgaca agcggttatt tgcaccaaat acgtaggtat ttggctagca aatatagagc
     6001 ttgccaagcc ctttagtgag ctttagtgag ctttacttat tgtacctttt taggtacaat
     6061 ggtattgcga acgatacgtc cgtaggacgt actgaaaggg tgggacttat gagcgaacaa
     6121 aatcaatatt tggctagcaa tcattctaaa aaatatacct atcgatccga gccaaaacaa
     6181 atcagttttc gagtgagcga atccgaattt gcaaagctga agcagtcagc tgaagctttg
     6241 caaatgagtg tgccggcttt cgtgaaagcc aaggcacaaa acgcgcgtct ggtgacaccg
     6301 aaagtggcgc cagacattgc tcaggcaatc gcgcgcgatt tagccaaagc tggcgggaat
     6361 atcaatcaaa ttgccaagtg gtgcaacaca caccaacacg acgttgatcc tggcgacgcc
     6421 caacgcttat ccgaaaacct aaaaatcatg caaaaggagc tacaaaagat atggcaacag
     6481 ttaaagtaag ccgaaccact tcttgtagcc gagcgatcaa ttacgccgaa ccacgagcaa
     6541 ccgtcaaaac agggataaac tgtgacattg attatgccaa aagcgaaatg aaacaaattc
     6601 gcatgctgta tggaaaagac gatcgtgtac aagcacactt attgatccag tcttttcgac
     6661 caggagaaat taccgctgaa aaagccaatc aactaggggc agagtatgcg aaaaaaatag
     6721 cccctgacca ccaaatcgcg atttacacac atacagacaa agaccatatt cataaccaca
     6781 ttgtcattaa ctccgtcaat ctggaaactg ggaacaaatt tcaagcccat ggtcaagcat
     6841 ttttaaacaa atgttatgac ctaaatgacg agatttgtct tgaacatgac ttaacaatca
     6901 ctgaacgagg gaagaaaccc gaaaaacgaa ccatgtctga aatcaaatta aaagaaaaaa
     6961 atgaacctgt ttggaaagac gaaattcggt ttgcgattga tcaaacaatg aaaaacaaaa
     7021 aaactcgaac gtataaccaa ttttgtgatt ctcttaaaac attcggcatt cattgcttga
     7081 atcgtgggaa aaattttact tacgaattga tccacaaaaa gaaaaaagta cgatccaaca
     7141 aactaggaaa ggattacgaa aaggagacga ttttacatga gctggacaga cgagaacgag
     7201 cctacaatca acaacgaacc aacgagctac gaagagctcg agctagcatt cgcgaaactg
     7261 caaaagaagt acgaagagaa caacgaactc ttacgactga acaaccggaa attagaccga
     7321 ctcgaagaaa ccatgaccaa cacgttgaaa gaggagcaga ctaccaacaa gaattatcgt
     7381 tctaaaacgt tacaagacat agaaaatgaa ataaagaaac aacgtgcttt tcttgcacct
     7441 tataccaaag aaagaataca aactacaatt gattcacaag tcaaaaaatt ccaccatgcg
     7501 ctattaaacc agcgaaataa agaacagaaa aaagtaatta tgcagcgatc aatggatatt
     7561 cttgccccct tatgttgctt aatcatgaca ctgttagcct tttggctggt atggggagaa
     7621 taaatagaaa tggcattcta tttaccctat ctactcattt ttgccagtat tttaggaatt
     7681 atttggctta gctataaggc ttatcaaaca agatattcat taaaaggctc taaaattcga
     7741 tttaagaggt ttttattgcc aggggttata tttacattat caatcgtttt aagtggttta
     7801 ttcggcgttt tagagggaaa taaacgtatt tcaagaagta ttttattagg ggatatcact
     7861 caaaagtatg aagcagcatg tgataaaaag aaaaaggagc aagtgttaac acaaaaaatg
     7921 gaagagttta ccacttgtta tgaggaaatg aacgatattt ttgtgaatca agaaaaacgt
     7981 ttgacggata aaaacatgga aaaactcact cgtctttatc aaaacttgcc agaaaaacag
     8041 caaaaagagg ttcaagacaa ctatgagcga accaaaaaag acgtgcaata ttttaaagat
     8101 acgcagacag aagaagcttg ttataattta ttcagtgata caatcccttt ttcaactagt
     8161 gaacaggaac gaaaagaacg gcaacaatca gttacctacg aacgttataa agcactgctt
     8221 cagcaagcaa cgaacataca aaatccaacg aaaaaagaaa cggcattaaa ttatctaaaa
     8281 agtgtcaagg aatggttaga tcagcaacag caaaattgaa tttgatcggc aatcatgtta
     8341 aactaaacat gcaaatgatc aaacattaga tacacgaaag ccccagagag gtcggattct
     8401 ctggggcttt ttttcaggtt gatgtcgcct ttattgattt tacttcttgt ttcggttatt
     8461 caaccaataa ctgaataaag agacacaaac ttctataaga agggggcaaa tgaacaattc
     8521 aaacattaga tacacctcct tccagtcgca agactgtcgg ataggcgaca agttattata
     8581 tcttaaaaaa ttaaacttgt gtggtgtttt taagtatcgc ttattttagg gttgttaggg
     8641 cttgccctga ccgcctgtaa ggcgctttaa agctagataa ggatttttat ccaagactgt
     8701 cttaaaacgg cttagataag cattcagaac cgctcaaagg cctctttacc aacatgtttt
     8761 tagcaaagga gcctttaggc gactgcgcag tacaatgtga gagcgtagcg atcacacctt
     8821 tttggcgacc gtaggaagca aggttttgga ttggcagttt tgaaaaatgc gcgaacctgt
     8881 ttaaagaaaa aagaagtggt ttttaggcgg gaaaattagc ccccctcttt ttatatctct
     8941 ttttaaacct ctttttaaac ctctttaccg taagctgtgt ccttactcta ccaatggata
     9001 cagaatgtta tgaaggacat tttgtctgtt tatgaaggac attttgtctg tttatgaagg
     9061 acattttgtc tgtttatgaa ggacattttg tctgtttgaa cgtcaaatat gaatgtcttg
     9121 acatttaaaa aatgtcattc ataattggat tgaggtgatt atatggctaa tgagattgta
     9181 aaataccacc atgaattaaa tacaattcct ttaagaaaat ttacaccggt tgaaatgaat
     9241 ttgttttttt ccatcgtgtc tagaatgcgt gaaaaagggg ataagacagt tcgttttacg
     9301 tttgaccaac taaaagattt aagtaactac aaagcaactg caaatgtacg atttgtagaa
     9361 gatttaaaag caacttatga aaaaatctta tctttacgct ttggtcgtag aagtgagtct
     9421 gggctttctt acgatatgtt tgtaatgttt acagaattta aaatcaacgg ggacgtagaa
     9481 aatccttacg tagatatacg aatttacgaa aaagcactgc ctttgttaaa taatttagac
     9541 gagtgggtac gctattcttt acaacaattc aacgagctac aaagtggcta tgcaaagact
     9601 atgtttcgtc tgttaaagca atacagaacc aaaggtttcg catatttctc caaagaagac
     9661 tttcttgaat tattagatat tcctaaaagt tataagcaac ctgatataga taaacgagtt
     9721 ataaaaccta tacgtcaaga actaactgca atcttcaaag gactaacaat caaaaagaaa
     9781 tacggaaaag gacggggaaa acctgttatt ggctatcaat ttacgttcaa acctgagatc
     9841 aaaaaagcag acgactttaa caaacatgca ccaaccaaag aagaccaaaa taaagcattg
     9901 gctttagctg ataaacaaaa agaatacgaa gaacccgcta caacaattaa cgaaaaaatc
     9961 aaacagaaga aacgagaact tgaactcctc caacaaatca aacaactaga aaaagagaaa
    10021 aaagctcttg aaaaacaaac agagtccgca ctttcggaaa aagagaaaaa aagtttatta
    10081 tctgatttag ataatttatt ccagtagcaa cctaggaggc aaccagtatg caaccacaag
    10141 aaacctttac aataaaggaa ttgtcagatc ttttaagatg agtaggcaag caatatcgaa
    10201 acatatccaa aaattagatt cttccatgat agctaaaaat gagcgtggtt ataaggttgt
    10261 attgcgttct ggagtattgc aacttgctcg caacttagac aatcaaaggt tgctagataa
    10321 gttgcataaa catgaacaaa acaacgataa aaatgcttat ataccaaaca aaaacgtggt
    10381 tgcaaacaag ttgctagatc agttgcgtga agaaaatgaa tttttacgag aacaacttgc
    10441 cctcaaagag caaaataatt ctcatataca aaaactactc gaccagcaac aacaactcac
    10501 cctgcaagcc aaccaacaaa tcgaaaaact tcaggagcaa ctgcagttga cttatacgga
    10561 agagtcttca gagaatagtt caacgactct ttctgaagca acagaaacaa tggacgaaca
    10621 aacaaagaat tggaaaaaat ggtggcagct atggaaatga agaaggaaat acttatgaca
    10681 aaaaaacact tgctattcgg ttctatccta ctaggaatat taagctttag cccaatgtta
    10741 ttatcagtgg ccaacaaagg aaataaaaag gggaactaaa actcaatcct aattactaca
    10801 acagtcaaaa tgaggaagta tttgtagaaa aacttgataa ataagaaaag actgctcgta
    10861 cgagcagtct ttctgaaaga gattatccta ttattaacct tagaaaagaa taataaatga
    10921 atatgagata tgtaaaaagc ataacaaaca caaacaaaaa taacaagtat ttaaaaaatg
    10981 accgatcaat attcacaaag aattagaaag aaacaaccgc agaaaaccaa gccttaaaaa
    11041 gagcttttaa cctagaccaa taaaaattca caaaatcagc gcctggatcc cttggtacag
    11101 gcgttacttg gtagtataca cattatgtct actagctgtt taaataactg atcttataat
    11161 tttatttaaa cgattatgaa ttcaaaacta ctttttatga cgttaatcat tgttatataa
    11221 taaattgtta tataataaaa tgttatataa taaaattact aagattattt aatataacaa
    11281 gcaattttaa aaaaacgaaa aggaaaaaca atcatgaaaa agaaagtatt aaaacattgt
    11341 gttattctag gaatattagg aacttgtcta gctggcatcg gtacaggaat aaaagttgat
    11401 gcagctactt actatggaaa tggtctttat tgtaacaaag aaaaatgttg ggtagattgg
    11461 aatcaagcta aaggagaaat tggaaaaatt attgttaatg gttgggttaa tcatggtcca
    11521 tgggcaccta gaaggtagtg gtagggaggt tattataatg gattttacta aagaagaaaa
    11581 acttttaaat gcaattagta aagtatacaa tgaagcaact atagatgact atcctgactt
    11641 aaaagaaaag ctctttcttt attctaaaga aatcagtgag ggaaaaagtg ttggtgaagt
    11701 tagtatgaaa ttaagtagtt ttcttggaag atatatttta aaacataaat ttggattacc
    11761 taaatcttta atagaattac aagaaattgt tagtaaggaa tctcaagtat atagaggatg
    11821 ggcttctatt ggtatttgga gttaattctt tggaataaaa tgaaaatggg actgaagaat
    11881 caattctgat tcatcagtcc cataaattat agagccgaaa aattatcata tttatagatt
    11941 tattaatagt aaatggcata agaatctttg aacaacgaaa aaaatgtagc tctacctaat
    12001 tattgatttg caacaatttc accgaaggtg aaacaagcga aactttacaa aaagtggcta
    12061 gcatttgcta gtcttttttg tattgtggag agtgggggga gggggattca aggggagctt
    12121 gcgccccttt gacaagcggt tatttgcacc aaatacgtag gtatttggct agcaaatata
    12181 gagcttgcca agccctttag tgagctttag tgagctttac ttattgtacc tttttaggta
    12241 caatggtatt gcgaacgata cgtccgtagg acgtactgaa agggtgggac ttatgagcga
    12301 acaaaatca
//
