LOCUS       AP026750                6673 bp    DNA     circular BCT 06-SEP-2022
DEFINITION  Escherichia coli PV06-71 plasmid pO165_PV06-71_4 O165:H25 DNA,
            complete sequence.
ACCESSION   AP026750
VERSION     AP026750.1
DBLINK      BioProject: PRJDB8147
            BioSample: SAMD00523003
            Sequence Read Archive: DRR401494, DRR401567
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Nakamura,K., Lee,K., Ooka,T., Taniguchi,I., Gotoh,Y., Ogura,Y.,
            Iyoda,S. and Hayashi,T.
  TITLE     Comparative genomic analysis of STEC O165:H25
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Nakamura,K., Taniguchi,I. and Hayashi,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2022) Contact:Keiji Nakamura Kyushu University,
            Faculty of Medical Sciences; Higashi-ku Maidashi 3-1-1, Fukuoka,
            Fukuoka 812-8582, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: unicycler v. 0.4.6
            Genome Coverage       :: 63x
            Sequencing Technology :: Illumina MiSeq; Nanopore MinION
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PV06-71"
                     /serovar="O165:H25"
                     /isolation_source="human feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pO165_PV06-71_4"
                     /geo_loc_name="Japan"
                     /collection_date="2006"
     gene            1497..1760
                     /locus_tag="PV0671_p4-1"
     CDS             1497..1760
                     /locus_tag="PV0671_p4-1"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDQ84941.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="hypothetical protein"
     gene            1830..1976
                     /locus_tag="PV0671_p4-2"
     CDS             1830..1976
                     /locus_tag="PV0671_p4-2"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:BAI39410.1"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin lysis protein"
                     /protein_id="BDQ84942.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin lysis protein"
     gene            complement(3021..3212)
                     /locus_tag="PV0671_p4-3"
     CDS             complement(3021..3212)
                     /locus_tag="PV0671_p4-3"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:ACB20375.1"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA I inhibition modulator protein Rom"
                     /protein_id="BDQ84943.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="RNA I inhibition modulator protein Rom"
     gene            3256..3603
                     /gene="mbeC"
                     /locus_tag="PV0671_p4-4"
     CDS             3256..3603
                     /gene="mbeC"
                     /locus_tag="PV0671_p4-4"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:BAI39404.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDQ84944.1"
                     /translation="MIPVKRERMLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPS
                     KTSGLPTLAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRD
                     EVRKQGERDDS"
                     /name="mbeC"
     gene            3593..5191
                     /gene="mbeA"
                     /locus_tag="PV0671_p4-5"
     CDS             3593..5191
                     /gene="mbeA"
                     /locus_tag="PV0671_p4-5"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:BAI39405.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeA"
                     /protein_id="BDQ84945.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERSREGARVLRGAPEEVRELID
                     ATPFAKKYTSGVLSFAEQTLPPGERERVMESFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELASGKRLQPYYDRADRPRINAWQTLVNHHYGLHDPNAPENRRTLVTPNNL
                     PKAKQEAAEAITRGVEALYHAGALKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGRN
                     LRLKGALYEQSFTNGDRLREETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRRY
                     QRTHSSGERTTGDAEQCRERAVQRHRERTCPAAGRAALEAGQRADTVPVADGALRHGRD
                     FSGVMGVDSVAGKPYCGEHPGYQGPGRDAGETPGKNMGCDVSRGQEREISGVAVRGEGG
                     KQLDGGEEERSKTGQGVSEYDRAGNALTERLRAIAAGLYAAAERLGERLHGFAAYVRAD
                     TTGERATEGTVRRLESAGAEISRAGGPLESVIYHEQQIRDEQERLARLERERARSWHEE
                     QEREVRVWRGPSMGM"
                     /name="mbeA"
     gene            5219..5440
                     /locus_tag="PV0671_p4-6"
     CDS             5219..5440
                     /locus_tag="PV0671_p4-6"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDQ84946.1"
                     /translation="MVPERVKNESGRDSGVSFLLLCFASLPDRAHSSASSRDVCVCHQL
                     KRWKKNSVMRFTQAKPALFTWLNSYAYG"
                     /name="hypothetical protein"
     gene            complement(5571..5816)
                     /locus_tag="PV0671_p4-8"
     CDS             complement(5571..5816)
                     /locus_tag="PV0671_p4-8"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDQ84947.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     BGC             1..6673
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2852..2936
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          2206..2390
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          3594..5188
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.872"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccagttccgg accggagtcg tccgggaaca aatattaaag atgtttacag tgcccctgta
       61 tcaccaaatc taccggacct ggtattaagt gtgggtcaga tgaatactcc ggttctgtct
      121 aatcccgaaa ttcaggaaga aggagttatt gctgagacag gtaattatgt tgaggctggt
      181 tatacgatgt ccagtaataa tcatgatgtc attgttcgtt ttcctgaagg cagtggtgtt
      241 tctccgttat atatttcaac tgtagagatt ctggacagta atggtctgag tcagcgtcag
      301 gaagccgaaa ataaagcaaa ggatgatttc agagtcaaga aagaagaagc ggtagcccgg
      361 gctgaagctg aaaaagcaaa agccgagtta tttagtaaag caggtgtgaa ccagcctcct
      421 gtatatacac aggaaatgat ggaaagggct aattcagtaa tgaatgagca gggagctctt
      481 gttctgaata acactgccag ttctgtacaa ctggcgatga ccggaactgg tgtctggact
      541 gctgctggtg atattgcagg caacataagc aagtttttca gtaatgctct ggagaaagta
      601 accatacccg aagtgagtcc cctgcttatg cggatttctc ttggtgctct gtggtttcat
      661 tcagaagaag ctggagcagg aagtgatatc gtgccggggc gaaatctgga ggcgatgttt
      721 tcgctgagtg ctcagatgtt ggctggacag ggcgtggtca ttgaacctgg tgcgacgagt
      781 gtaaatctgc ctgttcgtgg acaattgata aacagtaacg ggcaattagc tctggattta
      841 ctgaaaacag ggaatgaaag tatcccagct gcggttcctg ttcttaatgc tgtccgtgat
      901 acagcaacag gactggataa aatcacgtta ccagcagtag taggcgcacc ttcccggacg
      961 attctggtta atccggtacc acaaccttcg gtgccaacag atacaggtaa tcatcaaccc
     1021 gttccggtta caccagtgca tacaggaacg gaagtaaaac cggtcgaaat gccagtaacg
     1081 acgattactc ctgtttctga tgttggtgga ctacgagatt ttatttactg gcgtcctgat
     1141 gctgctggga ctggtgttga agctgtttat gtgatgctta atgaacctct ggattcaggg
     1201 cgattttcgc gtaaacaact tgataaaaaa tataaacatg ctggtgattt tggtattagt
     1261 gatacaaaaa agaatcgtga aactcttact aaattcaggg atgctattga ggagcattta
     1321 tcggataagg atacagtaga gaaaggaaca taccgaagag aaaaaggttc aaaagtttat
     1381 tttaatccta atacgatgaa tgtggttata attaagtcaa atggtgagtt cttatctggg
     1441 tggaaaataa atccagatgc ggataatggt cgaatttatt tagagacagg tgaactatga
     1501 acaagatggc aatgatcgat ttggcgaaat tatttttagc ttcgaaaatt acagcaattg
     1561 agttttcaga gcgaatttgt gttgaacgga gaagattgta tggtgttaag gatttgtctc
     1621 cgaatatatt aaattgtggg gaagagttgt ttatggctgc tgagcgattt gagcctgatg
     1681 cagatagggc tgattatgaa attgatgata atggacttaa ggttgaggtc cgatctatct
     1741 tggaaaaatt taaattataa tcatgagtga ttttaatatc tggcatttaa gtataaatgt
     1801 cagatattca ctcatgacaa ggagatgtca tgaaaaaaaa gataggggga tctatgattc
     1861 tcgttcttgc tgttttgtgt ctgactgctt gccaagctaa ctatgtaaga gatgttcagg
     1921 gtggaacaat tgctccatca tcatcttcga aactaatcgg ggtggcggtt cagtgaaaaa
     1981 tattaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgcc tgtaaacgaa
     2041 aaaacccgcc ttgtggcggg tccttccgtc cgatcagaag tcttcgacct cttctaatcg
     2101 gcggtggtaa ctggcttgga ggagcacagt caccaaaact gtcttttcag tttagcctta
     2161 actggttcat aacttcaaga gcaactcctc taaatcagtt accaatggct gctgccagtg
     2221 gcgttaagtc gtgtcttttc cgggttggcc ccaagagaac agttaccgga gaaggtgcag
     2281 cagtcgggct gaacgggggg ttcctgcata cagcccagcc cggagcgaac tgcctaagtc
     2341 gccccgagat accagaagac gtgagctatg agaaagcgcc acgtgtcccg caagggagaa
     2401 aggcggaaaa ggtatccggt aatcgatatg gcgggaacgt gggggcgcag gagggagcga
     2461 ttcaccggaa acggtgatga tctttaagcc acgtcgggtt tcgccaccgc tggcggattt
     2521 catggttgag cctcatggct cccacagatg cacagaaaaa gcgtctgttt tgtgaagtcc
     2581 gtcagggggg cggagcctat ggaaaaacgc cagcggcgcg actttttttg ccctttgcct
     2641 ttttgctcac atgttctttc cggccttatc ccctgattct gtggataaca ctatcaccgc
     2701 ttttgagtgg gcagataccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag
     2761 cgaggaagcg gaaaagcgcc tggacgagca ttttctcctt acgcatctgt gcggcatttc
     2821 acacccgcca tggcgcactt ttcatacaat tctcactgat gccatatgat taagccagta
     2881 tacactccgc tatcgctatg tgactgggtc atggctgcgc cccgacaccc gctaaaaccc
     2941 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg ctcacaggcc agctgtgacc
     3001 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat ccccgaagcg tgcgaggcag
     3061 ctgcggtaaa gctcgtcagc gtggtcgtga agcgcttcgc agatgtcagc ctggtcgtcg
     3121 gcgtccagtt cattcagttt ttccagcagg gtcagcgtct ggcttttgat gaagcgggcc
     3181 atgttaaggg cggttttttc ctgtttagtc acagttctgt ttctccatgt gtggggagtc
     3241 tgtcggaggt ttttaatgat accggtgaaa cgcgaaagga tgctcacgat acgggtcacg
     3301 gatgaggaac atgcgcggct gctggcacgt tgtgagggaa aacaacttgc gtcatggatg
     3361 cgtaaggtct gcctgggggc accgccatcg aaaacgtcag gtcttccgac actggcaccg
     3421 ccgctgttgc gccagttcgc ttcggtcggc aataacctga atcagatagc ccggaagata
     3481 aacagcggac agtggtcggg gcatgaccgg gttcatgtgg tggctgcact gatggccatc
     3541 ggacgtgaac tgagtgaact gagggatgag gtccgtaaac agggagaacg ggatgatagt
     3601 taagtttcat gccaggggaa aaggcggtgg cagtggtccg gttgattacc tgctgggccg
     3661 ggagcgtagc cgggaagggg cgagagttct gcggggtgct cccgaagagg tgcgggaact
     3721 catcgatgcc acgccgtttg cgaaaaaata cacctcgggg gttctgtctt ttgcagagca
     3781 gacactgcca ccgggagagc gtgaaagggt gatggagagc tttgagcggg tactgatgcc
     3841 cggtcttgaa aaaaatcagt acagcatcct gtgggtggag caccaggaca aggggcgtct
     3901 tgagctgaat tttgtcatcc cgaatatgga gctggcgagc ggaaaacggc tccagcccta
     3961 ttacgaccgg gcggaccgtc cgcgaatcaa tgcctggcag acactggtga atcaccatta
     4021 cgggctgcac gacccgaacg caccggagaa caggcggaca cttgtcaccc cgaataatct
     4081 gccgaaagcg aaacaggagg ccgcagaagc gattacgagg ggagtggagg ctctttacca
     4141 tgccggagcg ctaaaaacgc gtcaggatgt cactgaggcg cttacagcgg caggttttga
     4201 ggtggtgagg acaaccagga gcagtatcag tatcgccgac ccggagggcg ggcgtaatct
     4261 caggctgaaa ggggcgctgt atgagcaatc ttttacaaat ggggacagac ttcgagaaga
     4321 aactgaaaga gcgtgccgtc tctacagaga acatgctgaa cagcgagttc aggaagctcg
     4381 aagcgtctgt agacagggaa ctgaacttaa acgggcagaa aatcagagac gctatcagcg
     4441 cacacacagc agcggtgaaa gaacaactgg agacgctgaa cagtgccgtg aacgcgcagt
     4501 tcagcgccac agagaacgaa cttgcccggc ggcaggaaga gctgctctgg aagctggtca
     4561 aagggcggat actgtacccg tcgctgacgg cgctttgcgt cacggtcggg atttttctgg
     4621 cgtcatgggg gttgattcag tggcaggaaa gccgtattgc ggcgaacatc ctggctatca
     4681 gggaccagga agagacgctg gcgaaactcc gggaaaaaac atggggtgtg acgtatcacg
     4741 agggcaggaa cgggaaattt ctggtgttgc cgtccggggt gaagggggaa aacaactgga
     4801 cggtggagaa gaagaacgca gtaagactgg tcagggagta agtgagtatg acagagctgg
     4861 aaatgcactt actgaacgcc ttagagcaat tgcagcagga ctatacgcag cggctgaaag
     4921 actgggagag cgccttcacg gatttgcagc atatgttcga gctgacacga caggagaacg
     4981 ggcaactgag ggaacagtgc gacggcttga gtcagcaggt gcagagatta gccgggcagg
     5041 tggaccgctt gagtcggtta tttatcacga acagcagata agggatgaac aggaaaggct
     5101 ggcacgcctg gaacgggagc gtgcgcggtc ctggcatgaa gagcaggaac gggaagtacg
     5161 ggtatggcgt ggcccgtcaa tggggatgtg acggtgagtt gttccgggag gttgccagat
     5221 ggtaccggaa cgcgttaaaa acgaatccgg gcgcgattct ggcgtgtcgt ttttgttgct
     5281 gtgttttgcc tctttgccag atcgcgctca ttcctcagcc tcatcccgcg atgtctgcgt
     5341 atgtcatcaa ctgaaaagat ggaaaaaaaa cagtgtcatg aggtttacgc aggcaaaacc
     5401 agcgttattc acatggctga atagctatgc atacggttaa aatttatcag gtgcgatcgc
     5461 ggcagctttt cgggtggttt gttgccattt ttacctgtca gcagccgtga tcgcgctgaa
     5521 cgcatttcag cggtgcgtac aataaggggt tatgttaaat aacccttagc ttagcggcgg
     5581 cgcagtggtt gccaggtgaa ggtgtcatcc gggtcctgtc cggcagtcag tgtggcttct
     5641 cgatttttgc tggccgttcg ttcatcaaga atggctttaa gtatccgtgc ccggaggcgt
     5701 cgctttgttt cctgcggcat tttgtccttt ttcgtcagcc ccatggcacg agcttcagcg
     5761 gcaatggctt ctgccagtcg gcgctcttcg gcttcggtca gtctatggcg tttcatggtg
     5821 gtctccgggt gtttctctgg tgtcataaca gtttaactgt tggctctctc cttatgctaa
     5881 tcagccttct ttcatgcctg ttgccattga gtttaagaat gattggttgt ttttcttgag
     5941 cgttttcaca aaaacgtaaa tccagtgggt tgacaggatt tacgaggtga cgtaactttt
     6001 tactgtacat aaaaccagtg gttttatgta cagtttgttg tttttaacgc attgttttaa
     6061 aagtcaaaga ggtgttttta tgagtgatta cgaaggtagt ggtccgacag aaggtattga
     6121 ttatgggcac tcgatggtcg tgtggccgtc aacaggactg atttcgggtg gtgatgtgaa
     6181 accaggaggc tcatcaggta tcgctccatc catgcctccg ggatgggggg attacagccc
     6241 acagggtatc gcacttgtac aaagtgttct ttttccggga attattcgcc ggattattct
     6301 ggataaggaa cttgaagagg gagactggtc gggatggtct gtcagtgtgc atagcccttg
     6361 gggaaacgag aaagtttccg ctgcacgaac agttcttgag aatggtttac gtggtggttt
     6421 gccagaaccg tctcgcccgg ctgctgtttc ttttgcccgt ctggagcctg cttccggaaa
     6481 tgagcaaaaa attattcgtc ttatggttac acagcaactg gaacaggtaa cggatattcc
     6541 ggccagccag ttaccagcag cgggtaataa tgtaccggtg aaatatcgtc tgatggacct
     6601 tatgcagaac ggtacgcaat atatggctat tatcggaggt attccgatga cagtgccggt
     6661 agttgatgcc gtt
//
