LOCUS       AP026749                6715 bp    DNA     circular BCT 06-SEP-2022
DEFINITION  Escherichia coli PV06-71 plasmid pO165_PV06-71_3 O165:H25 DNA,
            complete sequence.
ACCESSION   AP026749
VERSION     AP026749.1
DBLINK      BioProject: PRJDB8147
            BioSample: SAMD00523003
            Sequence Read Archive: DRR401494, DRR401567
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Nakamura,K., Lee,K., Ooka,T., Taniguchi,I., Gotoh,Y., Ogura,Y.,
            Iyoda,S. and Hayashi,T.
  TITLE     Comparative genomic analysis of STEC O165:H25
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Nakamura,K., Taniguchi,I. and Hayashi,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2022) Contact:Keiji Nakamura Kyushu University,
            Faculty of Medical Sciences; Higashi-ku Maidashi 3-1-1, Fukuoka,
            Fukuoka 812-8582, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: unicycler v. 0.4.6
            Genome Coverage       :: 63x
            Sequencing Technology :: Illumina MiSeq; Nanopore MinION
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PV06-71"
                     /serovar="O165:H25"
                     /isolation_source="human feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pO165_PV06-71_3"
                     /geo_loc_name="Japan"
                     /collection_date="2006"
     gene            complement(296..1453)
                     /gene="hindVM"
                     /locus_tag="PV0671_p3-1"
     CDS             complement(296..1453)
                     /gene="hindVM"
                     /locus_tag="PV0671_p3-1"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:UniProtKB:P45000"
                     /codon_start=1
                     /transl_table=11
                     /product="modification methylase HindV"
                     /protein_id="BDQ84934.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="hindVM"
     gene            1541..2602
                     /gene="hindVRP"
                     /locus_tag="PV0671_p3-2"
     CDS             1541..2602
                     /gene="hindVRP"
                     /locus_tag="PV0671_p3-2"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:UniProtKB:P44999"
                     /codon_start=1
                     /transl_table=11
                     /product="putative type-2 restriction enzyme HindVP"
                     /protein_id="BDQ84935.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="hindVRP"
     gene            2993..3376
                     /locus_tag="PV0671_p3-3"
     CDS             2993..3376
                     /locus_tag="PV0671_p3-3"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDQ84936.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            3369..3575
                     /locus_tag="PV0671_p3-4"
     CDS             3369..3575
                     /locus_tag="PV0671_p3-4"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDQ84937.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(3843..5396)
                     /gene="mbeA"
                     /locus_tag="PV0671_p3-5"
     CDS             complement(3843..5396)
                     /gene="mbeA"
                     /locus_tag="PV0671_p3-5"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:BAG80510.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeA"
                     /protein_id="BDQ84938.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMESFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLSDNL
                     PETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKN
                     IRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRVQEARRVCQRGCDLKRDENQRRY
                     SPVHSLDREIAGKTQGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSW
                     REDTGEPGRNQEAGRDIAETEREDMGRGVCAGREKEISGHSARDVSRGDALSGEGLDTT
                     DGVIRNDRAGNTFAERLRAAATGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAV
                     ESAGAALERADRTLEPVIQRELEIREERLIQEREHALSLERERQPEIQERILDGPSLSL
                     "
                     /name="mbeA"
     gene            complement(5386..5742)
                     /gene="mbeC"
                     /locus_tag="PV0671_p3-6"
     CDS             complement(5386..5742)
                     /gene="mbeC"
                     /locus_tag="PV0671_p3-6"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:CBJ04269.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDQ84939.1"
                     /translation="MGVMIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGE
                     PVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRS
                     LRQVVREQGARDDC"
                     /name="mbeC"
     gene            5774..5965
                     /locus_tag="PV0671_p3-7"
     CDS             5774..5965
                     /locus_tag="PV0671_p3-7"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:ACB20375.1"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA I inhibition modulator protein Rom"
                     /protein_id="BDQ84940.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGEDL"
                     /name="RNA I inhibition modulator protein Rom"
     Typing          complement(6044..6128)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          6148..6694
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.455"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3853..5396)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.529"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5393..5742)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.96e-64"
ORIGIN
        1 tggcagcagc cactggtaac tggacaaggt ggattgagat attaagagtt cttgaagtgg
       61 tggcctaact gcggctacac tagaaggaca gtatttggta tctgcgctcc accaagccag
      121 ttaccttcgg aagaaagagt tggtagctct agattccggc aaacaaacca ccgttggtag
      181 cggtggtttt tttgtttaca ggcagcagat tacgcgcaga aaaaaaggat cacaagagga
      241 tcctttgatc tttttatccc ctccccctcc cctgagagac ataaattcgt atttattacc
      301 ttcgacaata ttcgtaatat aatttaaaag cccttcttaa ctgagacttc aggctaaccc
      361 caagcctgac aaacgattca agcctttcca gtttcataat aatttcatct atttcacagt
      421 tataaaaatc caccccctct gaatcaaaaa atgaaacacc tctgcaacat ctagatataa
      481 tatcgctaga ggttcttttt gttagtttct tttcattcag caaccacgcc ctaaagcctt
      541 ctttatcata aatacctttt acttgttttt tttcatactc catgattgtc accgcaacat
      601 attttgctaa ctcaaccgga acagcattac ctatcatttg ctccaaattt gtttttgcac
      661 ctttaaattt aaagtcttca ggaaatgttt gcaatcttgc tctttcaaag gttgttaatg
      721 gccttacatt ctcagaaata ctgacaggat cgcctgcatg ccctaaatat ccatcaggta
      781 tcggtctatt aacccctctt actgttggcg caggttcatc aatagagaaa atagcccttc
      841 tgttataatt tctcggatgt ctgtaataat actcaatccc caatgaatca ccaagataat
      901 ttctaactgt catactatct ttcgatatcc cgcacatcac ttcgttaagg ataaaaccat
      961 ccctcacgtc aagctttcca attacaaata atctttttct tttttgcgga acaccacact
     1021 tactggcatc caaaacaatt tctgttaacc cataccctga ttctttaaag attcctcgag
     1081 cttctaaata ggcaccactt cttaatgccc gatctacatt ttccatcaca aaccactttg
     1141 gctttattct ttttataatt tctgaaaatg agcgagttaa gttagctcta gcaccttcaa
     1201 ttcttaaacc tgcatgactg aagtcttggc aaggaggccc tccaataatc atatcgaagt
     1261 ctatatcttt aagctccctc accacatcat caacattgct caaatctagt ttttttatcg
     1321 gatggctaaa attatctctg taacattcaa tagcaggatc ccagaaatca taagcaccta
     1381 caacatcaaa cccccccttc aaaaagccta gggacaaccc cccacaccca ctaaaaagat
     1441 caattattct cataacataa aaaactcatt atcatatcac tccacatcat gatatagaaa
     1501 cacaccatag acgtcaataa attgacgtca ggagcaaaaa gtgagcaaaa tagaatctct
     1561 ttatggttta aaaaacacca acaggaaaga gagcgatctg tggggaaaga accaatttaa
     1621 cagttctttt cccacctctc tagcatgtta tatgagagac aacaaaatga atccgattta
     1681 tctttattta gataaggatt taaaagtgca tgccaaagaa gtatcttttg acttcgtttt
     1741 taacacaaca ttaaaaaata gtgatcttga atttcaattt gagaaaaaat atgaaccata
     1801 ccaacagtat gcttatgatg acataaaagg aatagatcta gttataaaag gaaacaatag
     1861 ttttttaaaa gcactagaaa taaaattaac cgtacttcca gatagcacga catataaaga
     1921 aaacgagagc gaatggagtt cagagattgt aataagacct gcaagcactt cttattgtgc
     1981 ccttggcata atgcactctt gcagcgataa attccatgaa ataagaaaaa tatttgaatc
     2041 cacatgcgaa agcatacagc actgggggaa tgaaactgaa gttctttcaa agaaagaaaa
     2101 tatattaaat tgcctaaaca ctttccaaga aaattttctt gattaccaga aacctttttt
     2161 aatgcaacca ttatggaaaa caaaaggcaa gagcccaata ttagatcaaa atgcctttga
     2221 catattcatc tggagtgatt ttgctttgtg tcgaaccttt attgatagat cctcatataa
     2281 caattcatct gccagcagac taatgagatc atcagccaga ctcgccagaa tggtgtatga
     2341 aattgcgagc aaaaacaaga caaacatcaa tagaatttat actgagatga actttggata
     2401 tcaaagtgat aaagagttct cgctaaacgg aaaaataaca aaagcttata tggatcaccc
     2461 aagaagaaca aaacccatcc tcccaccatc agtattaaaa gaaataattt taaatggcgg
     2521 gcaaaataaa ttaagcccag agagacgctt tgatcaaagc gtttattata ctgcatctca
     2581 cctgtttgat gaaataaagt aatttttctt ggtggctcgc cagccctgaa ttcgcccatc
     2641 aaaaatcagt agcggaacag gcatactaga catgctcgct tttacggcga gcatcttgac
     2701 ggagtttgag acgcaatgga acagcaacca actctatgca tcacaagctc aaaaatgatt
     2761 accatgcatt gaactctagt tacctttgcc aatgattgct cctggttgct tcatgcccgt
     2821 aaaggcaatt tcgacggtca ttacattgaa cataacatct cgaccgcgta ctgaacggtc
     2881 tcagccggat cttcgaaccc atctatcgta gaattgatac tctacctctg tgactaacaa
     2941 gacagttccg ctactcttct gagtattcat tcatcaaaaa gcaggatatc cagtgtcaaa
     3001 cattgttcat ctatctgata aaaaacaaaa aaacaaacca gttgatattt cttttgatga
     3061 ttacacagac aatctcagca aagggttcgt caaaaatctc agcaataaac aaaaaaaaga
     3121 attaattagt aaaatcaaag cgatacttaa atcgaggaat gtagcgttcc gttcgacgcc
     3181 gccctctgat tggaaagaga aaagtagaac ctttgcaatt ttctttcctg aaaaaaagat
     3241 tgtttttaca ttaggagtag tgaacaaatt aaagatagag gacggttgga taaatcataa
     3301 aatcagcgac acagaagatg ccgtcttatc tgttggaaaa ataatcaacc ttatagagga
     3361 ttcacagcat ggctaaatta cctcaagaaa agccgaaaga agtcatccaa gccggatttg
     3421 atgatgttaa aaaggcctat aaaaaaggcg ctaagactgt aaacgatacc tatgctgagc
     3481 aagcaggtcg tgacttacaa tcaggcgatg gtaaactaat ggctacctat cggcgcaata
     3541 aaaaagcgat agacaagtta gatgctgaag gttgatctga aaagtcagtc ttctgggcgg
     3601 acttttcgtc tctaagactg gctgcatatt gtatttacca taacatccgt tacgcacacc
     3661 gctaaaacgc gttcggtgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc
     3721 gaaaaactgc cgcgatcgcg cctgataaat tttaaccgca tgaataccta tgcaaccaga
     3781 tggtgcagac tacattagtc cgtcaaaatc cactgaacct gccatttttt atagtttcgc
     3841 catcacagcg acaacgaagg accatccagt atgcgttcct gtatttccgg ctgacgctcc
     3901 cgttccaggg ataacgcatg ttcgcgctcc tgtatcagcc gttcctctct tatctccagt
     3961 tctcgctgta taactggctc aagcgttctg tccgctcgct caagtgctgc acctgctgac
     4021 tcaactgcat gacccgctcg ttcagcatcg cgttgtcccg ttgcgtaagc gaaaacatct
     4081 tctgcaattc cacgaaggcg ctctcccatt cgttcagccg ctgcatatag tcctgttgca
     4141 gctgctctaa ggcgttcagc aaatgtgttt ccagctctgt cattccgtat cactccgtca
     4201 gttgtatcca gtccttcccc tgatagtgcg tcacctctgc tgacatccct tgcggaatga
     4261 ccagaaattt ccttctcccg tcctgcacaa actccacgcc ccatgtcttc gcgttcagtt
     4321 tctgcaatgt ctcttcctgc ttcctgattt cttccaggtt cgcctgtatc ctcccgccaa
     4381 gataccagag cgtcccgcca ctcgcggtaa acaggagaaa gactatcccc agtaacatca
     4441 tgcccgtatt ccctgccagc tttaacacgt ccttcctgtg ctgcatcatc gcctctttca
     4501 ccccttccct gtgtttttcc agcgatttct ctgtcgaggc tgtgaacagg gctatagcgt
     4561 ctctgatttt cgtctcgttt gaggtcacag cctcgctgac agactcgccg agcttcctga
     4621 actcgtcgtt cagcattttc tctgtagatt cggctctctc tttcagcttt ttctcgaact
     4681 ccgcgcccgt ctgtaaaaga ttgctcataa aacgctcctt tcagcctgat attcttcccg
     4741 ccgttcgggt ctgcaatgct gatgctgctt cgcgtcaccc tgaccacttc aagccccgcc
     4801 tctgtgagcg cctgaatcac atcctgacgg ccttttatct cccctacatg ataaagagcg
     4861 tctatcccgc gtgtgacgct ctcagcaagc gcctgtttcg tttccggcag gttatcagag
     4921 agagtcagta tccggcggtt ctccggggcg ttggggtcat gcagcccgta atggtgattc
     4981 accagtgtct gccaggcatc aattctcggc ctgtctgctc ggtcgtagta cggctggagg
     5041 cgttttccgg tctgtagctc catgttcgga atgacaaaat tcagctcaag acgccccttg
     5101 tcctggtgct ccacccacag aatgctgtac tgattttttt cgagaccggg catcagtacg
     5161 cgctcaaagc tctccatcac tttttcacgt cctcccggcg gcagttcctt ctccgcgaac
     5221 gacagaacac cggacgtgta tttcttcgca aatggcgtgg catcgatgag ttccctgact
     5281 tcttccggat taccctgaag caccgttgcc ccttcccggt tacgctccct tcccagcagg
     5341 taatcaaccg gaccactgcc accgcctttt cccctggcat gaaatttaac aatcatcccg
     5401 cgctccctgc tccctgacga cctgccgtaa gctgcgcaac tctccctcga tggccatcag
     5461 tgcggccacc acctgaaccc ggtcaccgga agaccactgc ccgctgttta ccttcctcgc
     5521 tgtctgattc aggttattcc cgatggcggc cagctgacgc agtaacggcg gtgccagtgt
     5581 cggcagcttc ccggaacggg caaccggctc acccaggcag acccgccgca tccataccgc
     5641 cagttgttta ccctcacagc gttccagtaa ccgggcatgt tcatcatcag taacccgtat
     5701 cgtgagcatc ctctcgcgtt tcatcggtat cattaccccc atgaacagaa atccccctta
     5761 cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag
     5821 aagccagaca ttaacgctgc tggagaaact caacgaactg gacgcagatg aacaggccga
     5881 tatttgtgaa tctcttcacg accacgccga tgagctttac cgcagctgcc tcgcgcgttt
     5941 cggtgatgac ggtgaagacc tctgacacat gcagctcccg gagacggtca cagcttgcct
     6001 gtgagcggat gccgggagca gacaagcccg tcagggcgcg tcagggggtt tcggggcgaa
     6061 gccctgaacc agtcacgtag cgatagcgga gtgtatactg gcttaactat gtggcatcag
     6121 tgcggattat aagaaaagag caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6181 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg
     6241 ttcggctgcg gcgagcggag tcggctcact caaaggcggt aatacggtta tccacagaat
     6301 caggggataa ggccggaaag aacatgtgag caaaaaggca aaggacaaca aaaggccgcg
     6361 ccgctggcgt ttttccatag gctccgcccc cctgacgagc atcacagaaa tcgacgctca
     6421 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6481 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6541 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag
     6601 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6661 ttatccggta actatcgtct tgagtccaac ccggaaagac acgacttaac gccac
//
