LOCUS       AP026654                4825 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA818 plasmid pAA818_3 DNA, complete sequence.
ACCESSION   AP026654
VERSION     AP026654.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466814
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA818"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA818_3"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(728..898)
                     /locus_tag="EfmAA818_34190"
     CDS             complement(728..898)
                     /locus_tag="EfmAA818_34190"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP89823.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(914..1447)
                     /locus_tag="EfmAA818_34200"
     CDS             complement(914..1447)
                     /locus_tag="EfmAA818_34200"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDP89824.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(1490..2419)
                     /gene="rep"
                     /locus_tag="EfmAA818_34210"
     CDS             complement(1490..2419)
                     /gene="rep"
                     /locus_tag="EfmAA818_34210"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP89825.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKREKKFII"
                     /name="rep"
     gene            complement(3302..3991)
                     /locus_tag="EfmAA818_34220"
     CDS             complement(3302..3991)
                     /locus_tag="EfmAA818_34220"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP89826.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3998..4288)
                     /locus_tag="EfmAA818_34230"
     CDS             complement(3998..4288)
                     /locus_tag="EfmAA818_34230"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748752.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP89827.1"
                     /translation="MNNTLNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTT
                     MDSQVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     Typing          complement(1469..2418)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.76"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gttacagcaa tatttgtata ttcctttttg ccccatcttg caactgcagc tcttgtcatg
       61 atatctgaag aatttgcaaa atcttccgca ctataaccat cctcatattt agttacgacc
      121 ataccatctt cagtgtactc aataactaca ttttcagtag cattgttttc gtttaatgat
      181 accacttggg aattttctga agcataaact atagtagttg acggtaaaac aactgcccca
      241 atagttaaca aagctacccc taaagtaaat tttttcatag aatttcctcc tgatacagaa
      301 aaatatatag aaaacgtttt ctttgtgaaa atcataaaac aaaaggtata ggcttgtcaa
      361 tattttattt agtaaagata atttatattt ttgataatgt ggtatagtta gaatgcatta
      421 ggaaaatatg ttatattaaa ttcttgttga cataatgtac actatgccaa gtagcccttg
      481 tatcaaccga tacagaggct cactttgtga ttttcgattg gtctaggtca aaagctcttt
      541 ttaagcttga ttttctgcgg ttgcttcttt ccgtttcttt gtgaattttt accagtcatt
      601 ttttactggg ttgttatttt taataaaata tgataaattt ttggtagcgc ttcataagcg
      661 cgttgctttt tcaagaataa acactcatta ttttttaact ggctgctcga aagagcagtc
      721 ttttttatta tttatctgtt tcttttacaa acaattcttc gttaggactg ttgtaataat
      781 ttggattcaa tttcaattca ccatgtttat tccctttatt gatcactgac aataacatcg
      841 gactgaatcc tagaattcct agtaaaatag aaccgaatag aagatttttt cttgtcatga
      901 atgttccctc cagttatttc caaagttgcc accatttttt ttcagtatca atttgtttgt
      961 ctattgtttc tgttgcttca gaaagagtcg ttgagtcatt ctctggagct tcttctgtat
     1021 aagtcaactg tagtttttct tgcagttttt cgatttgttt gttggcttgt agggtgagtt
     1081 gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctctg agggcaagtt
     1141 gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt gtaaccacgt
     1201 ttttgcttgg tatattaaca tttttatcgt tgtcttgttc gtactcgtgc aacctatcta
     1261 gcaacatttg gttgcctaag ttgcgagcaa gttgcaatac tccagaacgc aatacaacct
     1321 tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata tgtttcgata
     1381 ttgcttgcct actcatctta aaaagttctg acaattcctt tattgtaaag gtttcttgtg
     1441 gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat cagataataa
     1501 actttttttc cctttttttc gaaagtgcgg actctgtttg tttttcaaga gcttctttct
     1561 ctttttctag ttgtttgatt tgttggagga gttcaagttc tcgtttcttc tgtttgattt
     1621 tttcgttaat tgttgtagca ggttcttcgt attctttttg tttatcagct aaagccaatg
     1681 ctttattttg ttcttctttg gttggtgcat gtttattaaa atcatctgct tttttgattt
     1741 ctggtttaaa cgtaaattga tagccaacga caggttttcc tcgccctttt ccgtatttct
     1801 ttttgattga taatcctttg aagattgcgg ttaattcctg tcgtataggc tttataacac
     1861 gattatcaac gtttgcttgg ttgttccagt aactttttgg aatatctaat agttcatgaa
     1921 aatcctcttt agaaaaataa gcaaaacctt tagttcgata ttgttttagt aatcgaaaca
     1981 tagtctttgc atagctactt tgtaattcgt taaattgttg gagtgaatag cgaacccact
     2041 catctaaatt gtttagtaaa ggtaaagctt tttcgtaaac ttgtatgtct acatatggat
     2101 ctttagcagt tccacgtatt ttaaactcac taaacataac gaatctatct atatcgagcc
     2161 cattagcact tcttttacca aaacgtaaat ccattaattt atcataagtc atttctaaat
     2221 cattaatgaa acgattatta gcagttgctt tataattact taaatctttt agttggtcaa
     2281 aagtaagacg gactgtttta tcgccttttt cgcgcattct agacacaata gagaaaaata
     2341 gattcatttc aactggtgta aatttccgca acggaatggt atttaattca tgatggtatt
     2401 tcacgatttc atttgccaaa gatattcctc cttactcctt caatggatac agacgttata
     2461 aacaacgttt tgtctgtgat aatgcatatt ataacatacg tgagtgcaaa aaaagagttt
     2521 aaaagcagac aaaatgtagt ttaaaagcag acaaaatgta gtttaaaagc agacaaaatg
     2581 tagtttaaaa gcagacaaaa tgtagtttat aaaacgctgt atccattgaa ggagtaagga
     2641 cacagccgac ggtaaagagg tttaaaaaaa ggtttaaaaa gagattataa aagagggtga
     2701 caaaatttcc cgcctaaaaa ccacttcttt tttctaccaa caggttcgcg catttttcaa
     2761 aactgccaat tcaaaacctt gctccctacg gtcgctaaaa aagtgtgatc gctatgctct
     2821 cacattgtac tgcgcagtcg cctaaaggct cctttgctaa aaacaagtca gtaacggagg
     2881 tgggggaagc ctctgatagc tcgtctaagt cgttttaaga cagtcttggg taaaaaacct
     2941 tatcttgttc ttaaagcgcc ttacaggcgg tcagggcaag ccctaacaac ccgaaaataa
     3001 gggatactta aaaacaccac acagatttta ttttgtaagg tataataact tgtcgcctat
     3061 ccgacagtct tgcgactgga aggaggtgta tctaatgttt gaacggttca tttgccccct
     3121 tcttgtagaa gtttgtgtct ctttattcag ttattggttg aataaccgaa acaagaagta
     3181 aaatcaataa aggcgacatc aacctgaaaa aaagccccag agaatccgac ctctctgggg
     3241 ctttcgtgta tctaatgttt gatcatttgc aggttcagtt taacatgatt gctaatcaaa
     3301 ttcaattttg ttgttgctga tccaaccatt ccttgacact ttctagataa tttaatgccg
     3361 tttctttttt cgttggattt tgtatgtttg ttgcttgctg aagcagtgtt tcataacgtt
     3421 cgtatgtgac cgattgttgc cgttcttttc gttcttgttc actggttgaa aaagggattg
     3481 tatcgctaaa taagttacaa caagcttctt ctgtctgcgt atctttaaaa tattgcacgt
     3541 ctttttttac ttgctcatat ttttcttgta cctctttttg ctgtttttct ggtaagtttt
     3601 gataaaaacg agtgagtttt ttcatgtttt tatctgttaa acgtttctct tgatccacaa
     3661 aaatattgtt catgtcctca taacaagcgg taaattcctc cattttttgt gctaatgctt
     3721 gctctttttt ctttttatca catgctgctt catacttttg agtgatatcc cctaataaaa
     3781 tgcttcttga aacacgttta ttaccctcta aaacgccgaa taaaccactt aaaacgattg
     3841 ataaggcaaa tataaccccg accaataaaa atcgcttaaa tcgaatttta gagcctttta
     3901 atgaatatct tgtttgataa gccttatagc taagccaaat aattcctaaa atactggcag
     3961 aaatgagtag atagggtaaa tagaatgcca tgtctgttta ttctccccat accaaccaaa
     4021 aggctaataa tgttagtact aagcaacata agggggcaag aatatccatc gatcgttgaa
     4081 ttaaagcctt tttttgttct tggtttcgct ggtctaatag cgcttggtgg agttttttga
     4141 cttgtgaatc catcgtagtt tgtatccttt cttgggtata aggcgcaaga aaggcacgtt
     4201 gtctctttat ttcgttttcg atgtctttta acgttttaga gcgataattc ttgttggtgg
     4261 tctgttcctc gttcaacgtg ttgttcatgg tttcttcgag tcggtctaat ttccgattgt
     4321 tcaggcgtaa aagttcgttg ttctcttcgt acttcttctg cagtttcgcg aatgctagct
     4381 cgagctcttc gtagctcgtt ggttcgttgt tgattgtagg ttcgttctcg tctgtccagc
     4441 tcatgtaaaa tcgtctcctt ttcgtaatcc tttcctagtt tgttggatcg tacttttttc
     4501 tttttgtgga ccaattcgta agtaaaattt tttccgcgat tcaagcaatg aatgccgaat
     4561 gttttaagtg aatcacaaaa ttgggtatac gttcgagttt ttttgttttt cattgtttga
     4621 tcaatcgcaa accgaatttc gtctttccaa accggttcat ttttttcttt taatttaatt
     4681 tcagacatgg ttcgtttttc ggttttcttt cctcgttccg tgattgttaa gtcatgttca
     4741 agacaaatct cgtcatttag gtcataacat ttgtttaaaa atgcttgccc atgggcttga
     4801 aattttttcc cagtttcaag attaa
//
