LOCUS       AP026648                4369 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA610 plasmid pAA610_6 DNA, complete sequence.
ACCESSION   AP026648
VERSION     AP026648.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466813
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA610"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA610_6"
                     /geo_loc_name="Japan"
                     /collection_date="2017"
     gene            complement(104..523)
                     /locus_tag="EfmAA610_36070"
     CDS             complement(104..523)
                     /locus_tag="EfmAA610_36070"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86397.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(516..749)
                     /locus_tag="EfmAA610_36080"
     CDS             complement(516..749)
                     /locus_tag="EfmAA610_36080"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86398.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(752..1720)
                     /locus_tag="EfmAA610_36090"
     CDS             complement(752..1720)
                     /locus_tag="EfmAA610_36090"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP86399.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVGK
                     LNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQE
                     GKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPV
                     SFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRG
                     KATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLL
                     KRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
                     /name="replication initiation protein"
     gene            complement(2165..3571)
                     /locus_tag="EfmAA610_36100"
     CDS             complement(2165..3571)
                     /locus_tag="EfmAA610_36100"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86400.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSENEIEW"
                     /name="hypothetical protein"
     gene            complement(3794..4156)
                     /locus_tag="EfmAA610_36110"
     CDS             complement(3794..4156)
                     /locus_tag="EfmAA610_36110"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86401.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     Typing          complement(760..1629)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.164"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(2169..3571)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.094"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
       61 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
      121 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
      181 gaagcaaagt caatttttgt tggtcacgaa tcatttccgt taattcagaa atatgcctat
      241 ctttttccgc taattgttca cgcaaaaatt cattttcttt ttctaacggg aaatcattgc
      301 ctgcatcatt gaccggtaaa tcaccagtca attgaccacc ttttgaccga taaattgacc
      361 gcacaacacc aatcaattcc ttgtctaaca tgataacccc attatcttta taccgagcaa
      421 ggtcataacc tgcattgacc agttgattgc gcactgtatt tttagatata cccaactcat
      481 cagcaaggtt ttttatggtc attttttctg gcaaatcagt cattatcttc tccgttactt
      541 tcagctaccc cataaacata tttaacagca tgcttaggga taatgattgg ggaatcaatt
      601 ttattatttt tcaaaatcaa ctcatactgc gttacatcaa gtaacttacc ggacattttt
      661 ttgctatcca aaaaaacaac gacaacgtta tcacctgtta aattcaccaa ccaatttttc
      721 ttaccaaaat gcttttgttt ttctgccatt actattcctc ctctgtcaca tcaagtccct
      781 gtaaccaagt atccaattct ttttttaaat catctgctga ttcttcaaat gtttctcgta
      841 aatgattcgt caactcatca ttttgacttt ctcgctttaa taaatttcga tatttatatt
      901 tcgagtttct tgacaaaatc gcccaattag aatgattagc taacaatcca tcaatcataa
      961 ctttatccgt aggttttata tcagttggta aaaaatgtgg actcacaaag ctatccaaac
     1021 tttctttcac catagcatac cattccgtag ctttaccacg tcttaactgc atttctaacc
     1081 gccaccaagt attaatttct gaaggcacca cctttctttt catttcttgc tctatttttt
     1141 tattgtacaa acgaatttgc cgctcgcttg cccgactacc ccaatacacc gtttccagtt
     1201 ttcctgagcg tccataaatt aatttaaatg aaactggatc aataattcga tattgcgtga
     1261 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     1321 ctaaaaataa atcatgaata aaatttttca tggaatccgc taaaaatcga ctgattttat
     1381 taggattaaa atcaatccgc ccttttcctt cttgccattt agaaatttca atataggcaa
     1441 tattttcgtc atgttggtct aatagctgcc aaccattatt tccaaccgct tttaaatagc
     1501 cattgccttc atttacccgc ataagctgtt caaaatctaa tattaataca tcatgttgag
     1561 tatgataaaa aatattttga ttcaactttc caacaatggt aattcggtca atcgaccaat
     1621 ttaattttat agtttgtacc aaagtttgaa catttaaccg cctgttagac agggctgtta
     1681 atttttccca ctcattcacg attgttcttt ttttactcat attcagttcc tcctcaaaga
     1741 aaaaagagat ttttggaaag aatttccaga gttcacgtaa ctctaaaaat tcataaccat
     1801 caatctctag tcttgaagaa acctaaaaat attcgtataa tgaatttgag ttacggaact
     1861 agtgtggtta gtttccgtaa aggtcgagaa cgttggtagc gttgctcgac ttattttttt
     1921 gctctttttc cagttcattt gtttgctaac tcaataatag cacttctctt tagctatgca
     1981 aataccaaaa aaataaaacc gcaccctttg aaaagggcgc ccccaaactt cgctaacgct
     2041 aagttggcca aaaaatggac ttgccatttt ttagcaaatg tcagcttcag gggctagtgg
     2101 tttagccgtc aactccttat cctcggcaca aaccgcctcc ggtaccgttg actgcacatc
     2161 gtggctacca ctcaatctca ttctcagaac gctctaattt atgctgtgag acgttttgac
     2221 gataaaataa ggttttctcc tgctctaata cttctaattc ctcagaaagc cccatatcct
     2281 tttctgcaag accaaaacgg atagacaaac gagtgatcga tttttgaaat gcgtttttta
     2341 atcgctctaa acgctggcgt aaagagcgat tttcttcttt cagcttttta ttttccactt
     2401 gtaaatctgc taattgtttt ctaccagaca aatgacgctc ttcttccaat gtagctacat
     2461 actcatttaa ttctgtattt tgctcaagca tctgtaaata ttgctcactc atgttatcag
     2521 catttctctt ggcatttgtt gctctcgtag ctcgttgata caaatcaaca aaatcattcc
     2581 cagataaatg atacgaacca tcaggaagcc tcttgaaact ttctaaagga accatatctt
     2641 tttggaaaat gaccgccatc tcttttttta cattcagcat atttttcttc tcttcttcaa
     2701 tcttcgctct ctcagaagcc atcagagcct tatcacgctc gttttcttct ctttgcctat
     2761 aaatatacct caactcatca gaaacgctct cacgctcttt tacagccgtt tggcgcaatt
     2821 cagaaatttc actcatgatt tctttgtatt cgtgaatatc tttaattttg ttggtaccga
     2881 caatatccct tttccaaccg taatcattca ttaatcgttc tatagagtca acttcttgat
     2941 ttctaaaatt attgaaaagc actcgaccat tatctttatc agcgtgtatc ccttgttgaa
     3001 ccagtgcttt attaaagctc ggctgctttt gaacaccccg tttataacct tcagcaaccg
     3061 gcacaatatt caagtgcagg tgcggactta tttcgtcatt atgaataaca gcattataaa
     3121 cagccaaatt agggtttctc tcttgaaact ccttgtaata ctgaaccaaa attttattgg
     3181 caatcactcg attttgttca gcttgttgtt tcgatatgcc agtagaagaa ccatctgctc
     3241 gatttgtctg gaaatcatct aaaatgccta tttgcacaat aaattctcgt tgaggttcca
     3301 actttttatc cttgcgcact ttcgctaaat agtcctcgat ttttctatcg gctctttttt
     3361 gtttcgcatt atatttttct accacttcgc caaacaattc ctcatattta tctgctagat
     3421 tcgtctgctc taaataaata ttttcatccg atttcgtgaa gtcaatgtgc ttatgccaat
     3481 cattggtttt ctggcgatca gttaactcac gattattatg agaaatactt gtattcttcg
     3541 ttcctttttt aaatgaaata ctaaacccca agttaaaacc ctccttcgcg ctgtcgctaa
     3601 taaaaagatt gtaaaccttt ctgttactct ttgtcaaaga gtaaacattt tcctaattgt
     3661 gacatggatg tcacaattcc aaacgctctc cgagggtttt cgctgtcaca agtgacaacg
     3721 aaaaaaggag ccgaaagcgt atcacgctat caactccttt tatgctttat tttgcttcac
     3781 gcaaaataat cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac
     3841 tcatctgact tttcttattc ggtatcactc cgaataagtc gtcagattca tcgggataat
     3901 ctcgttcgct tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt
     3961 gttgctgatc gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gtttcaaacc
     4021 caaaacgctt gcgaagagcc ttgcccattg cagcattttc tttctcaatc aagcgttttt
     4081 tctgttggcg aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg
     4141 ctaaagtcat tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt
     4201 ttaactgcat aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg
     4261 tttgcgctaa atgcttatga acaaattcat tagtcaaaac tgaagatttt gctagatcag
     4321 cattgataac cgagttgaga acatccataa ttcgttcaag catttcact
//
