LOCUS       AP026647                4470 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA610 plasmid pAA610_5 DNA, complete sequence.
ACCESSION   AP026647
VERSION     AP026647.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466813
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA610"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA610_5"
                     /geo_loc_name="Japan"
                     /collection_date="2017"
     gene            complement(314..487)
                     /locus_tag="EfmAA610_36010"
     CDS             complement(314..487)
                     /locus_tag="EfmAA610_36010"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86391.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(564..731)
                     /locus_tag="EfmAA610_36020"
     CDS             complement(564..731)
                     /locus_tag="EfmAA610_36020"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86392.1"
                     /translation="MVKIPLIRENGQEPKRSAKKTYQAPQAKKVRVTSKKEKFFRTTFE
                     NLILIYIRSI"
                     /name="hypothetical protein"
     gene            complement(1172..1711)
                     /locus_tag="EfmAA610_36030"
     CDS             complement(1172..1711)
                     /locus_tag="EfmAA610_36030"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDP86393.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(1727..2149)
                     /locus_tag="EfmAA610_36040"
     CDS             complement(1727..2149)
                     /locus_tag="EfmAA610_36040"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86394.1"
                     /translation="MIFLYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPV
                     IGYRFTWKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHE
                     ADFKQQQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="hypothetical protein"
     gene            complement(2384..2578)
                     /locus_tag="EfmAA610_36050"
     CDS             complement(2384..2578)
                     /locus_tag="EfmAA610_36050"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86395.1"
                     /translation="MNLFFSIASRVREKGVDEIIFSFHDLKKLSGYTERGEQFIKDLSS
                     TYDKLLSINAWNDYGNTLT"
                     /name="hypothetical protein"
     gene            complement(3941..4327)
                     /locus_tag="EfmAA610_36060"
     CDS             complement(3941..4327)
                     /locus_tag="EfmAA610_36060"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86396.1"
                     /translation="MIVGASTPLNLFPFSARGIKVEAASSRYNHNHRGFTCYKMNYYVT
                     NKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVDAMNVFVRAANQGKGVQ
                     LTYNAHFSNTTSYQYNDYARYVIK"
                     /name="hypothetical protein"
     Typing          complement(1728..2661)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.085"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attttataaa acgtgttatt ttaaaggtta gtagacataa tgcatagtat accaagtagc
       61 ccttgtatca accgatacag gagctcactt tgtgattttt aattggtcta ggtcaaaagc
      121 tctttttaag gcttgatttt ctgcagttgc ttctttccgt ttctttgtga atttttacca
      181 gtcatttttt actggggtgt tatttttcat aaaatatgat aaatttttgg tagcgcttca
      241 taagcgcgtt gctttttcaa gaataaacac tcattatttt ttaactggct gctcgaaaga
      301 gcagtctttt ttattattta tccgtctctt ttacaaacaa ttcttcgtta ggactgttgt
      361 aataatttga atttaattta agttctccgt tggaattgtt ttttccaatt acggataata
      421 gcatggggct aaaacctaat attcctagag aaatagaacc cagtaccaac acagtttttt
      481 ttaacatagg ttatacctcc acttttttta acgctaccac ttgtatacac attttaaata
      541 ctagccttca gtttatcaaa tgtttaaatt gaacggatat aaatcaaaat taaattttca
      601 aaagttgttc taaaaaattt ttcttttttg gaagttactc taactttttt tgcttgtggt
      661 gcttggtatg ttttcttcgc cgaccgttta ggttcttgac cattttctct tataagtggg
      721 atttttacca cgttgagaga aaatggtcaa gaacctggga ggcggagaat gcccttaata
      781 ccaagcacca caagcctttc ccatacttta ttcgatgatc ccacgatgat ctcataatga
      841 ttctacaatg accctacgat gatcaaaatt ctcaaaaaaa ataaacgaaa tttcaaacga
      901 tttttttgaa attttgttaa aatcacattt gtttttttat gactttttca acaaaatcga
      961 ttagaattta ttttgttttt ccaccaaaaa ttgagtgaga tttcaaccta ttttaagcaa
     1021 taagtcacca tatagtttga tagtttacaa ctataaacat tgattgttta cagttgtaaa
     1081 ctattttttc tttctcataa aaatttgagt gatctcccca tcaggtagta caactttttc
     1141 ctctactatc cgaactaact tctagaaaat tttagagatt ggttctatta gatttccaaa
     1201 tttgccacca ctttttttca ataagtggtt gtgtattgtc tgcttttttt tcagaaaagg
     1261 cagttgattc ggttgttgga gtttcttctg tataagttaa atggagctgt tcttgtaggt
     1321 tctcaatttg ttggttcgct tgtagagtga gttgttgttg ctggtcgagt aatttttgca
     1381 tttgttttat ttgattatct ttttcttgta gctgtatatc ttttcgattt aactgttttt
     1441 ctaggaaaga aaggagtttt aattcggaaa cttggttggt gtttttatgg tttttggttt
     1501 ttgatttttc gccaactagt tttcgattta agtttgcgtt atcgactaaa aacattgatt
     1561 ttatagcttt ttgaccggtt tcgttaatca caaacttgtt tccgtttttt gcaaaccaaa
     1621 gttttcgatt tttgtcagaa acttttttgt taattgctgt tttactaact cctaattctt
     1681 ccgcaagttc tttgattgtt ttatctgcca taaatactcc ttaagcttaa tcgaataagt
     1741 tttgtaaatt tcctagtaaa tcttgtttga gtttgtttga aagttctcgt ttttctgcct
     1801 gtttttcttc agtttgttgt tgttgtttaa aatcagcttc atgagtgcct aagggaagat
     1861 ttagtattcg atctttagcg cgccattttt cttctcgtgt taattctcca ttgtgatcaa
     1921 tattaaataa ttttatacgt aaatcttctt gtttcccttt ggaaaaatcg tcagcatggt
     1981 taatttcagc tttccaagta aaacgataac cgataacagg tttacctcga cctttaccat
     2041 attttttacg aatagttaat cctttaaata agggagtgag ttcttcttta attggcctta
     2101 agataaattt gtcaatattt gacggactat tccaatatag gaatatcaag taaatcaaaa
     2161 aaaaaaattt ttctttggaa aattcagcct tgagttcgat attgttttaa taatcgaaac
     2221 atagtttttg aatatgtgct atccaaatta gtaaattgct ctagattaaa tcttgtccag
     2281 ttctttaatt gattaaaaag tcctttaaat tctggattaa ctgaaattat aacaagttgt
     2341 ttatctcgta taatgctata ttttgtgaag tgaaaaaatt tagttaagtt aaagtatttc
     2401 cataatcatt ccaagcatta atacttaata atttgtcata cgtgctacta agatctttta
     2461 taaattgttc cccacgttct gtataaccgc ttagtttttt caaatcgtga aatgaaaaaa
     2521 taatctcatc tacacctttt tcacgtacac gtgaagcaat tgaaaaaaat aagttcattt
     2581 cacgactatt aaatttttat taaattttct cagaggaata tgatttaatc tattttcata
     2641 ttttacaatt tcatttgcca taagcttctc ctacttttat aaagtatgaa atactttata
     2701 aaaatactca ataatgtcgg aaaacctact tgaaaagcgt agaaaaccta cttgaaaagc
     2761 gtagaaaacc tacttgaaaa gcgtagaaaa cctacttgaa aaaactctga attgcttggg
     2821 agagtaagga cacagccgac ataaaagaga tataaaaaga gatataaaaa gggatataaa
     2881 aaagagggga ggctaatttt cccgcctaaa aaatcactcc ttttttctct aaacaggttc
     2941 gcgaatttat caaaactgcc aattccaaac cttgctccct acggttcgct aaaaaggtgt
     3001 gatcgctctg ctctcacatt atactgcgca gtcgcctccg gctcctttgc taaaagccgt
     3061 tcataaggaa ccctttgaga agctctgagc gttcgtttaa gacattttaa gacagtccta
     3121 gtataaatcc cttatcttgt tcttaaaacg ccttacaggc ggtcagggca agccctaaca
     3181 accctaaaat tgtctaaatg ccctctaaaa ttcgatttaa gcactcttaa acctattctt
     3241 atatatttat atacttaggc gtttcaaacg tcttaaaacg caaattagag cttgataaag
     3301 atatttgctc gtgtgcctag acttttaaaa aagtcggcac acgccatgac aaagctgaaa
     3361 taaactgctt cagctttgtc atggcggatt cgctcacttg aaaaagtgtt tatttcgctc
     3421 gattattagc atatattttt tagctttttt gctagccaaa ttttgcatta atttgctcat
     3481 aagtcccacc ctttcagtac gtcctacgga cgtatcgttc gtaatgtgaa agtaccttta
     3541 aaggtacttt ctacaaaact cactaaaagg gtttggcaag ctctatattt gctagccaaa
     3601 tacctccgta tttggtgcaa ataaccgctt gtcaaagggg cgcaagctcc cccttgaatc
     3661 cccctcaaaa cccacgctcc acaatacaaa aagtggctag aaaatgctag ccactttttg
     3721 taaaatttag tatgatttat ttttagctat ttagagtttt tatttccgta aatacttcta
     3781 aaaattgcta agataaatgc tataggaatt agataaagaa tacttttttg taagcttata
     3841 ttgaaaatta gcctaaatat gaagaacata gtgattaaaa ctattattga aataattaca
     3901 gctaaaatct ttttattcat gagatagttt cactttctga ttattttatt acataacgag
     3961 cgtaatcatt gtattgatat gatgttgtat tgctaaagtg agcattatat gttaattgta
     4021 caccttttcc ttgatttgca gctcttacaa agacattcat tgcatcaaca gcactactaa
     4081 aagcatttgt aataacccat gttccaactc caccaatagg aataaaagat gcaatttgag
     4141 caggagtctt tagcttatta taattttttt tcaaatctct acacatttta ttagtaacat
     4201 aatagttcat tttatagcaa gtaaatccac gatgattatg attatatcgt gaacttgcag
     4261 cttccacttt tatacctcta gcactaaaag gaaacagatt taacggagta cttgctccaa
     4321 caatcatacc gaacaaaaat aaatagacaa gtaataattt taataatttt atttttcatt
     4381 gttacttcct ccttagatat aaaatcctag gaaagcgctt tctgtatata tttagaataa
     4441 tcagaaaatt aaatattgtc aagagggttt
//
