LOCUS       AP026646                6165 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA610 plasmid pAA610_4 DNA, complete sequence.
ACCESSION   AP026646
VERSION     AP026646.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466813
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA610"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA610_4"
                     /geo_loc_name="Japan"
                     /collection_date="2017"
     gene            39..326
                     /locus_tag="EfmAA610_35930"
     CDS             39..326
                     /locus_tag="EfmAA610_35930"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86383.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            767..1156
                     /gene="mobC"
                     /locus_tag="EfmAA610_35940"
     CDS             767..1156
                     /gene="mobC"
                     /locus_tag="EfmAA610_35940"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDP86384.1"
                     /translation="MGLMSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC"
     gene            1138..2052
                     /gene="mobA"
                     /locus_tag="EfmAA610_35950"
     CDS             1138..2052
                     /gene="mobA"
                     /locus_tag="EfmAA610_35950"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="BDP86385.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="mobA"
     gene            2000..2290
                     /locus_tag="EfmAA610_35960"
     CDS             2000..2290
                     /locus_tag="EfmAA610_35960"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748752.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86386.1"
                     /translation="MTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTT
                     IDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            2297..2986
                     /locus_tag="EfmAA610_35970"
     CDS             2297..2986
                     /locus_tag="EfmAA610_35970"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86387.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            3830..4774
                     /gene="rep"
                     /locus_tag="EfmAA610_35980"
     CDS             3830..4774
                     /gene="rep"
                     /locus_tag="EfmAA610_35980"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP86388.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEAQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="rep"
     gene            4795..5328
                     /locus_tag="EfmAA610_35990"
     CDS             4795..5328
                     /locus_tag="EfmAA610_35990"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDP86389.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            5343..5513
                     /locus_tag="EfmAA610_36000"
     CDS             5343..5513
                     /locus_tag="EfmAA610_36000"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP86390.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     BGC             968..6165
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3831..4774
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.894"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1139..2049
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgggcacct agaaggtagt ggtagggagg ttattataat ggattttact aaagaagaaa
       61 aacttttaaa tgcaattagt aaagtataca atgaagcaac tatagatgac tatcctgact
      121 taaaagaaaa gctctttctt tattctaaag aaatcagtga gggaaaaagt gttggtgaag
      181 ttagtatgaa attaagtagt tttcttggaa gatatatttt aaaacataaa tttggattac
      241 ctaaatcttt aatagaatta caagaaattg ttagtaagga atctcaagta tatagaggat
      301 gggcttctat tggtatttgg agttaattct ttggaataaa atgaaatggg actgaagaat
      361 caattctgat tcatcagtcc cataaattat agagccgaaa aattatcata tttatagatt
      421 tattaatagt aaatggcata agaatctttg aacaacgaaa aaaatgtagc tctacctaat
      481 tattgatttg caacaatttc accgaaggtg aaacaagcga aactttacaa aaagtggcta
      541 gcatttgcta gtcttttttg tattgtggag agtggggggg gagggggatt caagggggag
      601 cttgcgcccc tttgacaagc ggttatttgc accaaatacg taggtatttg gctagcaaat
      661 atagagcttg ccaagccctt tagtaagctt tagtgagctt tacttattgt acctttttag
      721 gtacaatggt attgcgaacg atacgtccgt aggacgtact gaaagggtgg gacttatgag
      781 cgaacaaaat caatatttgg ctagcaatca ttctaaaaaa tatacctatc gatccgagcc
      841 aaaacaaatc agttttcgag tgagcgaatc cgaatttgca aagctgaagc agtcagctga
      901 agctttgcaa atgagtgtgc cggctttcgt gaaagccaag gcacaaaacg cgcgtctggt
      961 gacaccgaaa gtggcgccag acattgctca ggcaatcgcg cgcgatttag ccaaagctgg
     1021 cgggaatatc aatcaaattg ccaagtggtg caacacacac caacacgacg ttgatcctgg
     1081 cgacgcccaa cgcttatccg aaaacctaaa aatcatgcaa aaggagctac aaaagatatg
     1141 gcaacagtta aagtaagccg aaccacttct tgtagccgag cgatcaatta cgccgaacca
     1201 cgagcaaccg tcaaaacagg gataaactgt gacattgatt atgccaaaag cgaaatgaaa
     1261 caaattcgca tgctgtatgg aaaagacgat cgtgtacaag cacacttatt gatccagtct
     1321 tttcgaccag gagaaattac cgctgaaaaa gccaatcaac taggggcaga gtatgcgaaa
     1381 aaaatagccc ctgaccacca aatcgcgatt tacacacata cagacaaaga ccatattcat
     1441 aaccacattg tcattaactc cgtcaatctg gaaactggga acaaatttca agcccatggt
     1501 caagcatttt taaacaaatg ttatgaccta aatgacgaga tttgtcttga acatgactta
     1561 acaatcactg aacgagggaa gaaacccgaa aaacgaacca tgtctgaaat caaattaaaa
     1621 gaaaaaaatg aacctgtttg gaaagacgaa attcggtttg cgattgatca aacaatgaaa
     1681 aacaaaaaaa ctcgaacgta taaccaattt tgtgattctc ttaaaacatt cggcattcat
     1741 tgcttgaatc gtgggaaaaa ttttacttac gaattgatcc acaaaaagaa aaaagtacga
     1801 tccaacaaac taggaaagga ttacgaaaag gagacgattt tacatgagct ggacagacga
     1861 gaacgagcct acaatcaaca acgaaccaac gagctacgaa gagctcgagc tagcattcgc
     1921 gaaactgcaa aagaagtacg aagagaacaa cgaactctta cgactgaaca accggaaatt
     1981 agaccgactc gaagaaacca tgaccaacac gttgaaagag gagcagacta ccaacaagaa
     2041 ttatcgttct aaaacgttac aagacataga aaatgaaata aagaaacaac gtgcttttct
     2101 tgcaccttat accaaagaaa gaatacaaac tacaattgat tcacaagtca aaaaattcca
     2161 ccatgcgcta ttaaaccagc gaaataaaga acagaaaaaa gtaattatgc agcgatcaat
     2221 ggatattctt gcccccttat gttgcttaat catgacactg ttagcctttt ggctggtatg
     2281 gggagaataa atagaaatgg cattctattt accctatcta ctcatttttg ccagtatttt
     2341 aggaattatt tggcttagct ataaggctta tcaaacaaga tattcattaa aaggctctaa
     2401 aattcgattt aagaggtttt tattgccagg ggttatattt acattatcaa tcgttttaag
     2461 tggtttattc ggcgttttag agggaaataa acgtatttca agaagtattt tattagggga
     2521 tatcactcaa aagtatgaag cagcatgtga taaaaagaaa aaggagcaag tgttaacaca
     2581 aaaaatggaa gagtttacca cttgttatga ggaaatgaac gatatttttg tgaatcaaga
     2641 aaaacgtttg acggataaaa acatggaaaa actcactcgt ctttatcaaa acttgccaga
     2701 aaaacagcaa aaagaggttc aagacaacta tgagcgaacc aaaaaagacg tgcaatattt
     2761 taaagatacg cagacagaag aagcttgtta taatttattc agtgatacaa tccctttttc
     2821 aactagtgaa caggaacgaa aagaacggca acaatcagtt acctacgaac gttataaagc
     2881 actgcttcag caagcaacga acatacaaaa tccaacgaaa aaagaaacgg cattaaatta
     2941 tctaaaaagt gtcaaggaat ggttagatca gcaacagcaa aattgaattt gatcggcaat
     3001 catgttaaac taaacatgca aatgatcaaa cattagatac acgaaagccc cagagaggtc
     3061 ggattctctg gggctttttt tcaggttgat gtcgccttta ttgattttac ttcttgtttc
     3121 ggttattcaa ccaataactg aataaagaga cacaaacttc tataagaagg gggcaaatga
     3181 acaattcaaa cattagatac acctccttcc agtcgcaaga ctgtcggata ggcgacaagt
     3241 tattatatct taaaaaatta aacttgtgtg gtgtttttaa gtatcgctta ttttagggtt
     3301 gttagggctt gccctgaccg cctgtaaggc gctttaaagc tagataagga tttttatcca
     3361 agactgtctt aaaacggctt agataagcat tcagaaccgc tcaaaggcct ctttaccaac
     3421 atgtttttag caaaggagcc tttaggcgac tgcgcagtac aatgtgagag cgtagcgatc
     3481 acaccttttt ggcgaccgta ggaagcaagg ttttggattg gcagttttga aaaatgcgcg
     3541 aacctgttta aagaaaaaag aagtggtttt taggcgggaa aattagcccc cctcttttta
     3601 tatctctttt taaacctctt tttaaacctc tttaccgtaa gctgtgtcct tactctacca
     3661 atggatacag aatgttatga aggacatttt gtctgtttat gaatgacatt ttgtctgttt
     3721 atgaaggaca ttttgtctgt ttatgaagga cattttgtct gtttgaacgt caaatatgaa
     3781 tgtcttgaca tttaaaaaat gtcattcata attggattga ggtgattata tggctaatga
     3841 gattgtaaaa taccaccatg aattaaatac aattccttta agaaaattta caccggttga
     3901 aatgaatttg tttttttcca tcgtgtctag aatgcgtgaa aaaggggata agacagttcg
     3961 ttttacgttt gaccaactaa aagatttaag taactacaaa gcaactgcaa atgtacgatt
     4021 tgtagaagat ttaaaagcaa cttatgaaaa aatcttatct ttacgctttg gtcgtagaag
     4081 tgagtctggg ctttcttacg atatgtttgt aatgtttaca gaatttaaaa tcaacgggga
     4141 cgtagaaaat ccttacgtag atatacgaat ttacgaaaaa gcactgcctt tgttaaataa
     4201 tttagacgag tgggtacgct attctttaca acaattcaac gagctacaaa gtggctatgc
     4261 aaagactatg tttcgtctgt taaagcaata cagaaccaaa ggtttcgcat atttctccaa
     4321 agaagacttt cttgaattat tagatattcc taaaagttat aagcaacctg atatagataa
     4381 acgagttata aaacctatac gtcaagaact aactgcaatc ttcaaaggac taacaatcaa
     4441 aaagaaatac ggaaaaggac ggggaaaacc tgttattggc tatcaattta cgttcaaacc
     4501 tgagatcaaa aaagcagacg actttaacaa acatgcacca accaaagaag cccaaaataa
     4561 agcattggct ttagctgata aacaaaaaga atacgaagaa cccgctacaa caattaacga
     4621 aaaaatcaaa cagaagaaac gagaacttga actcctccaa caaatcaaac aactagaaaa
     4681 agagaaaaaa gctcttgaaa aacaaacaga gtccgcactt tcggaaaaag agaaaaaaag
     4741 tttattatct gatttagata atttattcca gtagcaacct aggaggcaac cagtatgcaa
     4801 ccacaagaaa cctttacaat aaaggaattg tcagatcttt ttaagatgag taggcaagca
     4861 atatcgaaac atatccaaaa attagattct tccatgatag ctaaaaatga gcgtggttat
     4921 aaggttgtat tgcgttctgg agtattgcaa cttgctcgca acttagacaa tcaaaggttg
     4981 ctagataagt tgcataaaca tgaacaaaac aacgataaaa atgcttatat accaaacaaa
     5041 aacgtggttg caaacaagtt gctagatcag ttgcgtgaag aaaatgaatt tttacgagaa
     5101 caacttgccc tcaaagagca aaataattct catatacaaa aactactcga ccagcaacaa
     5161 caactcaccc tgcaagccaa ccaacaaatc gaaaaacttc aggagcaact gcagttgact
     5221 tatacggaag agtcttcaga gaatagttca acgactcttt ctgaagcaac agaaacaatg
     5281 gacgaacaaa caaagaattg gaaaaaatgg tggcagctat ggaaatgaag aaggaaatac
     5341 ttatgacaaa aaaacacttg ctattcggtt ctatcctact aggaatatta agctttagcc
     5401 caatgttatt atcagtggcc aacaaaggaa ataaaaaagg ggaactaaaa ctcaatccta
     5461 attactacaa cagtcaaaat gaggaagtat ttgtagaaaa acttgataaa taagaaaaga
     5521 ctgctcgtac gagcagtctt tctgaaagag attatcctat tattaacctt agaaaagaat
     5581 aataaatgaa tatgagatat gtaaaaagca taacaaacac aaacaaaaat aacaagtatt
     5641 taaaaaatga ccgatcaata ttcacaaaga attagaaaga aacaaccgca gaaaaccaag
     5701 ccttaaaaag agcttttaac ctagaccaat aaaaattcac aaaatcagcg cctggatccc
     5761 ttggtacagg cgttacttgg tagtatcgac attatgtcta ctagctgttt aaataactga
     5821 tcttataatt ttatttaaac gattatgaat tcaaaactac tttttatgac gttaatcatt
     5881 gttatataat aaaatgttat ataataaaat tactaagatt atttaatata acaagcaatt
     5941 ttaaaaaaac gaaaaggaaa aacaatcatg aaaaagaaag tattaaaaca ttgtgttatt
     6001 ctaggaatat taggaacttg tctagctggc atcggtacag gaataaaagt tgatgcagct
     6061 acttactatg gaaatggtct ttattgtaac aaagaaaaat gttgggtaga ttggaatcaa
     6121 gctaaaggag aaattggaaa aattattgtt aatggttggg ttaat
//
