LOCUS       AP026640                2058 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA290 plasmid pAA290_7 DNA, complete sequence.
ACCESSION   AP026640
VERSION     AP026640.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466812
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA290"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA290_7"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
     gene            239..460
                     /locus_tag="EfmAA290_34630"
     CDS             239..460
                     /locus_tag="EfmAA290_34630"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82787.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            461..718
                     /locus_tag="EfmAA290_34640"
     CDS             461..718
                     /locus_tag="EfmAA290_34640"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82788.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(854..1456)
                     /locus_tag="EfmAA290_34650"
     CDS             complement(854..1456)
                     /locus_tag="EfmAA290_34650"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002919940.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="BDP82789.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="plasmid replication protein"
     gene            complement(1523..1678)
                     /locus_tag="EfmAA290_34660"
     CDS             complement(1523..1678)
                     /locus_tag="EfmAA290_34660"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82790.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="hypothetical protein"
     Typing          621..796
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(927..1456)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ttacaggctc cattctcgct tctcttacgt gttttaacga ttgcgccgat aactaaacga
       61 aatagacgct aaatcgctta aaacgcaaaa tagacccttt ttagagcatt gattttttct
      121 actagtcatt tcgataatat aaaattgacc acaacacaga aaacttatat tataagctta
      181 tgtcgtaagt ccgacgtact cacgtaatat agacggacgt taaatggaag gagcataaat
      241 gagagtgata aaatcagaaa aattcacttt ggccgaaatt atgacgacca gcgaacttgc
      301 acaagaactt gattacaacc aagcttacgt tttgcgctta gcgaaagaac atctgactga
      361 aggaaaagaa tatcgctctg ccggtagaag aaactatctt ttcagcccag aagcccttaa
      421 aaaactagaa aaagtcataa ccaccaaaga aggtgagtaa atgcctaaag aaacaactat
      481 caacgttgaa caactagaaa aattgctaga atcatattcc aatgatttta tcgaattgac
      541 cggcggaaaa gtcgaagaaa ccaccgacct ctttgaccta cttctcacaa atattctgga
      601 attaaactct gaagaacaaa aagagcataa gggaaaactg atagcacctt cctacaggaa
      661 ggtggctaag tcggctgtgc caactggttt tcttacaaaa caaccttata ttttttgata
      721 tcacaaaaaa atccattttg gacctatttt cggtcataat atagtaccta cttttggtca
      781 tagtttcgtc cgtagttttg gtcatagtga ctaagacttt cacgcacaaa aaaagagctg
      841 aaattaacag ctcttatcct ttatttattt ttgaatttac acttccacca tgacgattcg
      901 attttatata ttgcccgata atataagctg aattatcaca aagaagcgga aaccaatcat
      961 caaatctttc tgacattgca tagtcaatta aatctttgat ttcagtgata ttcttagaat
     1021 caacaaacga catcatctct ctaatcaact catatcgttc tgcactcgtt actgacaaat
     1081 aactagcaat ttctgcacca ccatgagcga taatatcact tttagcatat tgaaattttt
     1141 ctggattatc catatgagca aaatatcgca ccataccttt tgcattagca catttttgcg
     1201 ggtttggaga ccgaagcttt tgagttatct cacgtatttg atcatacgtc tttttaccgc
     1261 taaacattat caaaacatgc caatgaggct ttttcaattc accgtcagga ttcacatctt
     1321 tatcatgaag tggactctca acccaaggaa catgtaactc atctaaaaac tccctccaat
     1381 tttcaggagc cgattcagga tagactacaa acgtccaatt ccttgtgcgt tcatctttag
     1441 tttttctact cgtcatgcta taattacctt aattgaacaa cagtctaaca gcaaaagcag
     1501 tgcgccaaca ctaatgcttt tgctattttt tttgctcatt ttcctttccg ttagcattaa
     1561 cccaaattgt aataagacca gacttactaa gacccatttg tttcgcaatt ttatcaagat
     1621 tatctgccac ttccttagta agcgttacaa cgactctctt tttttcatta ctgatcataa
     1681 ttcgtatcct ccaacgactt tttcttaaaa accatagtaa caaaaaagta actaaggtgc
     1741 aacagtttag atactaatta gcgaataata ggccttaaac ccttggcatt actgattttg
     1801 tcaaaaaaaa acgctgaaac ccttgggaga gtaaggctaa acagctatcg accaatgacc
     1861 aatgaccaat aaggggggtg tcgtagtccc cccctcctcc gcccaattca taaacaccaa
     1921 aaccaaaaaa caaacctcag ggctccgccc cgaaccccga cctccttcta ccgaaatata
     1981 tgcaggcgcc acgcttacta tttcgttcag ctatcggctt ttgcaatcta acacgaccgt
     2041 ttcgctacca gtcgcctt
//
