LOCUS       AP026639                4362 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA290 plasmid pAA290_6 DNA, complete sequence.
ACCESSION   AP026639
VERSION     AP026639.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466812
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA290"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA290_6"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
     gene            complement(697..1059)
                     /locus_tag="EfmAA290_34570"
     CDS             complement(697..1059)
                     /locus_tag="EfmAA290_34570"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82781.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(1063..1371)
                     /locus_tag="EfmAA290_34580"
     CDS             complement(1063..1371)
                     /locus_tag="EfmAA290_34580"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82782.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(1380..1799)
                     /locus_tag="EfmAA290_34590"
     CDS             complement(1380..1799)
                     /locus_tag="EfmAA290_34590"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82783.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(1792..2025)
                     /locus_tag="EfmAA290_34600"
     CDS             complement(1792..2025)
                     /locus_tag="EfmAA290_34600"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82784.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(2028..2996)
                     /locus_tag="EfmAA290_34610"
     CDS             complement(2028..2996)
                     /locus_tag="EfmAA290_34610"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP82785.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVGK
                     LNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQE
                     GKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPV
                     SFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRG
                     KATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLL
                     KRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
                     /name="replication initiation protein"
     gene            complement(3441..3998)
                     /locus_tag="EfmAA290_34620"
     CDS             complement(3441..3998)
                     /locus_tag="EfmAA290_34620"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82786.1"
                     /translation="MASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYH
                     LSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQL
                     ADLQVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEK
                     TLFYRQNVSQHKLERSENEIEW"
                     /name="hypothetical protein"
     Typing          complement(2036..2905)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.164"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(3..568)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="6.17e-121"
     Typing          complement(3445..4040)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.985"
                     /coverage="99.0"
                     /evalue="3.18e-152"
ORIGIN
        1 cattatgaat aacagcatta taaacagcca aattagggtt tctctcttga aactccttgt
       61 aatactgaac caaaatttta ttggcaatca ctcgattttg ttcagcttgt tgtttcgata
      121 tgccagtaga agaaccatct gctcgatttg tctggaaatc atctaaaatg cctatttgca
      181 caataaattc tcgttgaggt tccaactttt tatccttgcg cactttcgct aaatagtcct
      241 cgatttttct atcggctctt ttttgtttcg cattatattt ttctaccact tcgccaaaca
      301 attcctcata tttatctgct agattcgtct gctctaaata aatattttca tccgatttcg
      361 tgaagtcaat gtgcttatgc caatcattgg ttttctggcg atcagttaac tcacgattat
      421 tatgagaaat acttgtattc ttcgttcctt ttttaaatga aatactaaac cccaagttaa
      481 aaccctcctt cgcgctgtcg ctaataaaaa gattgtaaac ctttctgtta ctctttgtca
      541 aagagtaaca ttttcctaat tgtgacatgg atgtcacaat tccaaacgct ctccgagggt
      601 tttcgctgtc acaagtgaca acgaaaaaag gagccgaatg cgtatcacgc tatcaactcc
      661 ttttatgctt tattttgctt cacgcaaaat aatcgcttat cgtaaattca cgtcattaaa
      721 tattgacgta aatttactct cactcatctg acttttctta ttcggtatca ctccgaataa
      781 gtcgtcagat tcatcgggat aatctcgttc gcttggcgct gatttttcat cagcaagcca
      841 ctcattatcc actgtttttt cgtgttgctg atcgtcagaa attgacgata accaccgctc
      901 aaaatcagca aaagtttcaa acccaaaacg cttgcgaaga gccttgccca ttgcagcatt
      961 ttctttctca atcaagcgtt ttttctgttg gcgaagtttc gcttgttctt tttttaattt
     1021 ttcttcttgc gctttaatat ctgctaaagt cattcccata aactacatct ccttttcttt
     1081 ggaattaata acggaaattt ggtttaactg cataacatga ctcattaatt tcaaccaaaa
     1141 ttctttttct gattcatcag ttgtttgcgc taaatgctta tgaacaaatt cattagtcaa
     1201 aactgaagat tttgctagat cagcattgat aaccgagttg agaacatcca taattcgttc
     1261 aagcatttca ctataattat cttcatccgg caaattcaaa taatcagcta accgttcata
     1321 catcaattca attctatcga ctgtttcttc tctcaaatca attttatcca tgtcacacct
     1381 cacttccaga attgccacca ttttcgattt tttaattctt gattttctag taacaactga
     1441 tcgttttttt gttgctgaag caaagtcaat ttttgttggt cacgaatcat ttccgttaat
     1501 tcagaaatat gcctatcttt ttccgctaat tgttcacgca aaaattcatt ttctttttct
     1561 aacgggaaat cattgcctgc atcattgacc ggtaaatcac cagtcaattg accacctttt
     1621 gaccgataaa ttgaccgcac aacaccaatc aattccttgt ctaacatgat aaccccatta
     1681 tctttatacc gagcaaggtc ataacctgca ttgaccagtt gattgcgcac tgtattttta
     1741 gatataccca actcatcagc aaggtttttt atggtcattt tttctggcaa atcagtcatt
     1801 atcttctccg ttactttcag ctaccccata aacatattta acagcatgct tagggataat
     1861 gattggggaa tcaattttat tatttttcaa aatcaactca tactgcgtta catcaagtaa
     1921 cttaccggac atttttttgc tatccaaaaa aacaacgaca acgttatcac ctgttaaatt
     1981 caccaaccaa tttttcttac caaaatgctt ttgtttttct gccattacta ttcctcctct
     2041 gtcacatcaa gtccctgtaa ccaagtatcc aattcttttt ttaaatcatc tgctgattct
     2101 tcaaatgttt ctcgtaaatg attcgtcaac tcatcatttt gactttctcg ctttaataaa
     2161 tttcgatatt tatatttcga gtttcttgac aaaatcgccc aattagaatg attagctaac
     2221 aatccatcaa tcataacttt atccgtaggt tttatatcag ttggtaaaaa atgtggactc
     2281 acaaagctat ccaaactttc tttcaccata gcataccatt ccgtagcttt accacgtctt
     2341 aactgcattt ctaaccgcca ccaagtatta atttctgaag gcaccacctt tcttttcatt
     2401 tcttgctcta tttttttatt gtacaaacga atttgccgct cgcttgcccg actaccccaa
     2461 tacaccgttt ccagttttcc tgagcgtcca taaattaatt taaatgaaac tggatcaata
     2521 attcgatatt gcgtgataaa ctcatcaggc acatcaataa gatcgcaggc aacatccgct
     2581 cgtgaaaaat ggggctctaa aaataaatca tgaataaaat ttttcatgga atccgctaaa
     2641 aatcgactga ttttattagg attaaaatca atccgccctt ttccttcttg ccatttagaa
     2701 atttcaatat aggcaatatt ttcgtcatgt tggtctaata gctgccaacc attatttcca
     2761 accgctttta aatagccatt gccttcattt acccgcataa gctgttcaaa atctaatatt
     2821 aatacatcat gttgagtatg ataaaaaata ttttgattca actttccaac aatggtaatt
     2881 cggtcaatcg accaatttaa ttttatagtt tgtaccaaag tttgaacatt taaccgcctg
     2941 ttagacaggg ctgttaattt ttcccactca ttcacgattg ttcttttttt actcatattc
     3001 agttcctcct caaagaaaaa agagattttt ggaaagaatt tccagagttc acgtaactct
     3061 aaaaattcat aaccatcaat ctctagtctt gaagaaacct aaaaatattc gtataatgaa
     3121 tttgagttac ggaactagtg tggttagttt ccgtaaaggt cgagaacgtt ggtagcgttg
     3181 ctcgacttat ttttttgctc tttttccagt tcatttgttt gctaactcaa taatagcact
     3241 tctctttagc tatgcaaata ccaaaaaaat aaaaccgcac cctttgaaaa gggcgccccc
     3301 aaacttcgct aacgctaagt tggccaaaaa atggacttgc cattttttag caaatgtcag
     3361 cttcaggggc tagtggttta gccgtcaact ccttatcctc ggcacaaacc gcctccggta
     3421 ccgttgactg cacatcgtgg ctaccactca atctcattct cagaacgctc taatttatgc
     3481 tgtgagacgt tttgacgata aaataaggtt ttctcctgct ctaatacttc taattcctca
     3541 gaaagcccca tatccttttc tgcaagacca aaacggatag acaaacgagt gatcgatttt
     3601 tgaaatgcgt tttttaatcg ctctaaacgc tggcgtaaag agcgattttc ttctttcagc
     3661 tttttatttt ccacttgtaa atctgctaat tgttttctac cagacaaatg acgctcttct
     3721 tccaatgtag ctacatactc atttaattct gtattttgct caagcatctg taaatattgc
     3781 tcactcatgt tatcagcatt tctcttggca tttgttgctc tcgtagctcg ttgatacaaa
     3841 tcaacaaaat cattcccaga taaatgatac gaaccatcag gaagcctctt gaaactttct
     3901 aaaggaacca tatctttttg gaaaatgacc gccatctctt tttttacatt cagcatattt
     3961 ttcttctctt cttcaatctt cgctctctca gaagccatca gagccttatc acgctcgttt
     4021 tcttctcttt gcctataaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac
     4081 agccgtttgg cgcaattcag aaatttcact catgatttct ttgtattcgt gaatatcttt
     4141 aattttgttg gtaccgacaa tatccctttt ccaaccgtaa tcattcatta atcgttctat
     4201 agagtcaact tcttgatttc taaaattatt gaaaagcact cgaccattat ctttatcagc
     4261 gtgtatccct tgttgaacca gtgctttatt aaagctcggc tgcttttgaa caccccgttt
     4321 ataaccttca gcaaccggca caatattcaa gtgcaggtgc gg
//
