LOCUS       AP026638                6158 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA290 plasmid pAA290_5 DNA, complete sequence.
ACCESSION   AP026638
VERSION     AP026638.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466812
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA290"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA290_5"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
     gene            complement(158..847)
                     /locus_tag="EfmAA290_34490"
     CDS             complement(158..847)
                     /locus_tag="EfmAA290_34490"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82773.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(854..1144)
                     /locus_tag="EfmAA290_34500"
     CDS             complement(854..1144)
                     /locus_tag="EfmAA290_34500"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748752.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82774.1"
                     /translation="MTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTT
                     IDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(1092..2006)
                     /gene="mobA_2"
                     /locus_tag="EfmAA290_34510"
     CDS             complement(1092..2006)
                     /gene="mobA_2"
                     /locus_tag="EfmAA290_34510"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="BDP82775.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="mobA_2"
     gene            complement(1988..2368)
                     /gene="mobC_2"
                     /locus_tag="EfmAA290_34520"
     CDS             complement(1988..2368)
                     /gene="mobC_2"
                     /locus_tag="EfmAA290_34520"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDP82776.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_2"
     gene            complement(2814..3101)
                     /locus_tag="EfmAA290_34530"
     CDS             complement(2814..3101)
                     /locus_tag="EfmAA290_34530"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82777.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     gene            complement(3776..3946)
                     /locus_tag="EfmAA290_34540"
     CDS             complement(3776..3946)
                     /locus_tag="EfmAA290_34540"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82778.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            complement(3961..4494)
                     /locus_tag="EfmAA290_34550"
     CDS             complement(3961..4494)
                     /locus_tag="EfmAA290_34550"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDP82779.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(4515..5459)
                     /gene="rep"
                     /locus_tag="EfmAA290_34560"
     CDS             complement(4515..5459)
                     /gene="rep"
                     /locus_tag="EfmAA290_34560"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP82780.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="rep"
     Typing          complement(4516..5459)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1096..2006)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc gacatcaacc
       61 tgaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtatcta atgtttgatc
      121 atttgcatgt ttagtttaac atgattgccg atcaaattca attttgctgt tgctgatcta
      181 accattcctt gacacttttt agataattta atgccgtttc ttttttcgtt ggattttgta
      241 tgttcgttgc ttgctgaagc agtgctttat aacgttcgta ggtaactgat tgttgccgtt
      301 cttttcgttc ctgttcacta gttgaaaaag ggattgtatc actgaataaa ttataacaag
      361 cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc tcatagttgt
      421 cttgaacctc tttttgctgt ttttctggca agttttgata aagacgagtg agtttttcca
      481 tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt tcctcataac
      541 aagtggtaaa ctcttccatt ttttgtgtta acacttgctc ctttttcttt ttatcacatg
      601 ctgcttcata cttttgagtg atatccccta ataaaatact tcttgaaata cgtttatttc
      661 cctctaaaac gccgaataaa ccacttaaaa cgattgataa tgtaaatata acccctggca
      721 ataaaaacct cttaaatcga attttagagc cttttaatga atatcttgtt tgataagcct
      781 tatagctaag ccaaataatt cctaaaatac tggcaaaaat gagtagatag ggtaaataga
      841 atgccatttc tatttattct ccccatacca gccaaaaggc taacagtgtc atgattaagc
      901 aacataaggg ggcaagaata tccattgatc gctgcataat tacttttttc tgttctttat
      961 ttcgctggtt taatagcgca tggtggaatt ttttgacttg tgaatcaatt gtagtttgta
     1021 ttctttcttt ggtataaggt gcaagaaaag cacgttgttt ctttatttca ttttctatgt
     1081 cttgtaacgt tttagaacga taattcttgt tggtagtctg ctcctctttc aacgtgttgg
     1141 tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag tcgtaagagt tcgttgttct
     1201 cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag ctcttcgtag ctcgttggtt
     1261 cgttgttgat tgtaggctcg ttctcgtctg tccagctcat gtaaaatcgt ctccttttcg
     1321 taatcctttc ctagtttgtt ggatcgtact tttttctttt tgtggatcaa ttcgtaagta
     1381 aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt taagagaatc acaaaattgg
     1441 ttatacgttc gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg aatttcgtct
     1501 ttccaaacag gttcattttt ttcttttaat ttgatttcag acatggttcg tttttcgggt
     1561 ttcttccctc gttcagtgat tgttaagtca tgttcaagac aaatctcgtc atttaggtca
     1621 taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt tgttcccagt ttccagattg
     1681 acggagttaa tgacaatgtg gttatgaata tggtctttgt ctgtatgtgt gtaaatcgcg
     1741 atttggtggt caggggctat ttttttcgca tactctgccc ctagttgatt ggctttttca
     1801 gcggtaattt ctcctggtcg aaaagactgg atcaataagt gtgcttgtac acgatcgtct
     1861 tttccataca gcatgcgaat ttgtttcatt tcgcttttgg cataatcaat gtcacagttt
     1921 atccctgttt tgacggttgc tcgtggttcg gcgtaattga tcgctcggct acaagaagtg
     1981 gttcggctta ctttaactgt tgccatatct tttgtagctc cttttgcatg atttttaggt
     2041 tttcggataa gcgttgggcg tcgccaggat caacgtcgtg ttggtgtgtg ttgcaccact
     2101 tggcaatttg attgatattc ccgccagctt tggctaaatc gcgcgcgatt gcctgagcaa
     2161 tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct ttcacgaaag
     2221 ccggcacact catttgcaaa gcttcagctg actgcttcag ctttgcaaat tcggattcgc
     2281 tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta gaatgattgc
     2341 tagccaaata ttgattttgt tcgctcataa gtcccaccct ttcagtacgt cctacggacg
     2401 tatcgttcgc aataccattg tacctaaaaa ggtacaataa gtaaagctca ctaaagctca
     2461 ctaaagggct tggcaagctc tatatttgct agccaaatac ctacgtattt ggtgcaaata
     2521 accgcttgtc aaaggggcgc aagctccccc ttgaatcccc ctccccccca ctctccacaa
     2581 tacaaaaaag actagcaaat gctagccact ttttgtaaag tttcgcttgt ttcaccttcg
     2641 gtgaaattgt tgcaaatcaa taattaggta gagctacatt tttttcgttg ttcaaactta
     2701 tgccatttac tattaataaa tctataaata tgataatttt tcggctctat aatttatggg
     2761 actgatgaat cagaattgat tcttcagtcc cattttcatt ttattccaaa gaattaactc
     2821 caaataccaa tagaagccca tcctctatat acttgagatt ccttactaac aatttcttgt
     2881 aattctatta aagatttagg taatccaaat ttatgtttta aaatatatct tccaagaaaa
     2941 ctacttaatt tcatactaac ttcaccaaca ctttttccct cactgatttc tttagaataa
     3001 agaaagagct tttcttttaa gtcaggatag tcatctatag ttgcttcatt gtatacttta
     3061 ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca ttataataac ctccctacca
     3121 ctaactacct tctaggtgcc catggaccat gattaaccca accattaaca ataatttttc
     3181 caatttctcc tttagcttga ttccaatcta cccaacattt ttctttgtta caataaagac
     3241 catttccata gtaagtagct gcatcaactt ttattcctgt accgatgcca gctagctaga
     3301 ataacacaat gttttaatac tttctttttc atgattgttt ttccttttcg tttttttaaa
     3361 attgcttgtt atattaaata atcttagtaa ttttattata taacatttta ttatataaca
     3421 atgattaacg tcataaaaag tagttttgaa ttcataatcg tttaaataaa attataagat
     3481 cagttattta aacagctagt agacataatg tgtatactac taccaagtaa cgcctgtacc
     3541 aagggatcca ggcgctgatt ttgtgaattt ttattggtct aggttaaaag ctctttttaa
     3601 ggcttggttt tctgcggttg tttctttcta attctttgtg aatattgatc gtacttgtta
     3661 tttttgtttg tgtttgttat gctttttaca tatctcatat tcattttatc ttttctaagg
     3721 ttaataatag aggataatct ctttcagaaa gactgctcgt acgagcagtc ttttcttatt
     3781 tatcaagttt ttctacaaat acttcctcat tttgactgtt gtagtaatta ggattgagtt
     3841 ttagttcccc ttttttattt cctttgttgg ccactgataa taacattggg ctaaagctta
     3901 atattcctag taggatagaa ccgaatagca agtgtttttt tgtcataagt atttccttct
     3961 tcatttccat agctgccacc attttttcca attctttgtt tgttcgtcca ttgtttctgt
     4021 tgcttcagaa agagtcgttg aactattctc tgaagactct tccgtataag tcaactgcag
     4081 ttgctcctga agtttttcga tttgttggtt ggcttgcagg gtgagttgtt gttgctggtc
     4141 gagtagtttt tgtatatgag aattattttg ctctttgagg gcaagttgtt ctcgtaaaaa
     4201 ttcattttct tcacgcaact gatctagcaa cttgtttgca accacgtttt tgtttggtat
     4261 ataagcattt ttatcgttgt tttgttcatg tttatgcaac ttatctagca acctttgatt
     4321 gtctaagttg cgagcaagtt gcaatactcc agaacgcaat acaaccttat aaccacgctc
     4381 atttttagct atcatggaag aatctaattt ttggatatgt ttcgatattg cttgcctact
     4441 catcttaaaa agatctgaca attcctttat tgtaaaggtt tcttgtggtt gcatactggt
     4501 tgcctcctag gttgctactg gaataaatta tctaaatcag ataataaact ttttttctct
     4561 ttttccgaaa gtgcggactc tgtttgtttt tcaagagctt ttttctcttt ttctagttgt
     4621 ttgatttgtt ggaggagttc aagttctcgt ttcttctgtt tgattttttc gttaattgtt
     4681 gtagcgggtt cttcgtattc tttttgttta tcagctaaag ccaatgcttt attttggtct
     4741 tctttggttg gtgcatgttt gttaaagtcg tctgcttttt tgatctcagg tttgaacgta
     4801 aattgatagc caataacagg ttttccccgt ccttttccgt atttcttttt gattgttagt
     4861 cctttgaaga ttgcagttag ttcttgacgt ataggtttta taactcgttt atctatatca
     4921 ggttgcttat aacttttagg aatatctaat aattcaagaa agtcttcttt ggagaaatat
     4981 gcgaaacctt tggttctgta ttgctttaac agacgaaaca tagtctttgc atagccactt
     5041 tgtagctcgt tgaattgttg taaagaatag cgtacccact cgtctaaatt atttaacaaa
     5101 ggcagtgctt tttcgtaaat tcgtatatct acgtaaggat tttctacgtc cccgttgatt
     5161 ttaaattctg taaacattac aaacatatcg taagaaagcc cagactcact tctacgacca
     5221 aagcgtaaag ataagatttt ttcataagtt gcttttaaat cttctacaaa tcgtacattt
     5281 gcagttgctt tgtagttact taaatctttt agttggtcaa acgtaaaacg aactgtctta
     5341 tccccttttt cacgcattct agacacgatg gaaaaaaaca aattcatttc aaccggtgta
     5401 aattttctta aaggaattgt atttaattca tggtggtatt ttacaatctc attagccata
     5461 taatcacctc aatccaatta tgaatgacat tttttaaatg tcaagacatt catatttgac
     5521 gttcaaacag acaaaatgtc cttcataaac agacaaaatg tccttcataa acagacaaaa
     5581 tgtccttcat aaacagacaa aatgtccttc ataacattct gtatccattg gtagagtaag
     5641 gacacagctt acggtaaaga ggtttaaaaa gaggtttaaa aagagatata aaaagagggg
     5701 ggctaatttt cccgcctaaa aaccacttct tttttcttta aacaggttcg cgcatttttc
     5761 aaaactgcca atccaaaacc ttgcttccta cggtcgcaaa aaaggtgtga tcgctacgct
     5821 ctcacattgt actgcgcagt cgcctaaagg ctcctttgct aaaaacatgt tggtaaagag
     5881 gcctttgagc ggttctgaat gcttatctaa gccgttttaa gacagtcttg gataaaaatc
     5941 cttatctagc tttaaagcgc cttacaggcg gtcagggcaa gccctaacaa ccctaaaata
     6001 agcgatactt aaaaacacca cacaagttta attttttaag atataataac ttgtcgccta
     6061 tccgacagtc ttgcgactgg aaggaggtgt atctaatgtt tgaattgttc atttgccatt
     6121 gttcataatt gttcatttgc ccccttctta tagaagtt
//
